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Critical differences between two classical surgical approaches for middle cerebral artery occlusion-induced stroke in mice.

Abstract：

BACKGROUND:Stroke is the third leading cause of death and the leading cause of long-term disability in North America. On average, someone in the US has a stroke every 45 s, and worldwide, stroke claims 15 million lives each year. Therefore, reliable stroke models are vital to the production of effective new therapies for the treatment of this devastating cerebral vascular accident. NEW METHOD:Middle cerebral artery occlusion (MCAo) is considered to be the most clinically relevant surgical model of ischemic stroke, in which a variety of methods may be employed to block the MCA (the most common being through insertion of a monofilament). In this study, we have compared two different approaches that are currently used arbitrarily in various laboratories worldwide: one involving insertion of a monofilament via the common carotid artery (Koizumi et al.) and one via the external carotid artery (Longa et al.). RESULTS AND COMPARISONS WITH EXISTING METHODS:We assessed various parameters, including: mortality rates, neurological scores, inflammation levels, cellular trafficking (using intravital microscopy) and infarct volumes in mice after using each of the two approaches. We found that the Longa method produced a greater, and robust, inflammatory response, versus the Koizumi method. CONCLUSIONS:In conclusion, we suggest that the Longa method is superior for the study of both short and long-term outcomes of ischemic stroke. These results have considerable implications on stroke model selection for researchers.

journal_name

J Neurosci Methods

journal_title

Journal of neuroscience methods

authors

Smith HK,Russell JM,Granger DN,Gavins FN

doi

10.1016/j.jneumeth.2015.04.008

subject

Has Abstract

pub_date

2015-07-15 00:00:00

pages

99-105

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(15)00161-2

journal_volume

249

pub_type

杂志文章

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