Abstract：
:We describe a novel method for retrogradely labeling specific neuronal populations using voltage-sensitive dyes. Styryl dyes were injected into the ventral roots of the isolated embryonic chick spinal cord. After waiting several hours, the dye labeled motoneurons and autonomic preganglionic neurons. Neuronal cell bodies, dendrites and axons were labeled; we presume that the dye traveled either by retrograde transport or by diffusion within the membrane of the axon to which the dyes were initially applied. Using either a photodiode array or a photomultiplier, fluorescence changes could be recorded from motoneurons following antidromic or synaptic activation. Several characteristics of the fluorescence changes were measured indicating that the signals did indeed reflect changes in the motoneuron membrane potential. The best labeling and optical signals were obtained using the relatively hydrophobic dyes di-8-ANEPPQ and di-12-ANEPEQ. In the great majority of cases these dyes responded with an increase in fluorescence of 1-3% (delta F/F) in response to synaptic or antidromic depolarization of the motoneurons. We anticipate that these techniques should be useful in the mapping of activity patterns and connectivity in neural networks within a defined population of neurons.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Wenner P,Tsau Y,Cohen LB,O'Donovan MJ,Dan Ydoi
10.1016/s0165-0270(96)00108-2subject
Has Abstractpub_date
1996-12-28 00:00:00pages
111-20issue
2eissn
0165-0270issn
1872-678Xpii
S0165027096001082journal_volume
70pub_type
杂志文章abstract：:We present a client-server application for the distributed multivariate analysis of time series using standard PCs. We here concentrate on analyses of multichannel EEG/MEG data, but our method can easily be adapted to other time series. Due to the rapid development of new analysis techniques, the focus in the design o...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.09.002
更新日期：2006-04-15 00:00:00
abstract：:We investigated whether conscious rats exhibit hyperalgesia to noxious mechanical stimuli during tail reperfusion following transient tail ischaemia. Mechanical stimuli were delivered by three mechanical algometers, differing in contact area, geometry and rate of application. Tail ischaemia was induced by applying a p...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00313-7
更新日期：2000-11-30 00:00:00
abstract：:The most direct evaluation of human brain activity has been obtained from intracranial electrodes placed either on the surface of the brain or inserted into the brain to record from deep brain structures. Currently, the placement of intracranial electrodes implies transcranial surgery, either through a burr hole or a ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.12.027
更新日期：2013-03-30 00:00:00
abstract：BACKGROUND:In neuroscience, relating the spiking activity of individual neurons to the local field potential (LFP) of neural ensembles is an increasingly useful approach for studying rhythmic neuronal synchronization. Many methods have been proposed to measure the strength of the association between spikes and rhythms ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.05.004
更新日期：2016-08-30 00:00:00
abstract：:The human movement analysis panel (HMAP) measures separable components of arm motion and simple and complex finger coordination. HMAP testing takes 30min to administer. In separate experiments we have validated the HMAP against the standard grooved pegboard and measures of gait speed, and demonstrated important learni...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.06.015
更新日期：2007-09-30 00:00:00
abstract：:The calculation of volume fractions in nervous tissue is a method often used in neuroanatomy. In ontogenetic studies and problems concerning aging, as well as after experimental procedures, volume fractions are calculated in order to quantify the results. Grey level index (GLI) and grey cell coefficient (GCC) are diff...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(82)90014-0
更新日期：1982-07-01 00:00:00
abstract：BACKGROUND:Electroencephalogram (EEG) signals are often corrupted with unintended artifacts which need to be removed for extracting meaningful clinical information from them. Typically a priori knowledge of the nature of the artifacts is needed for such purpose. Artifact contamination of EEG is even more prominent for ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.04.006
更新日期：2016-07-15 00:00:00
abstract：:A simple method of generating precisely defined pulsed linear acceleration stimuli was developed and used to study vestibular responses to cranial acceleration. Electromechanical shakers were coupled to a stimulus platform. The platform, in turn, was used to couple stimuli to the skull. Movements were controlled by a ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90094-0
更新日期：1989-03-01 00:00:00
abstract：BACKGROUND:No satisfactory neurophysiological test for nociceptive afferents is available to date. Laser stimuli present risks of skin damage, whilst electrical stimulation through specially designed electrodes is not selective enough. NEW METHOD:We present a new electrode designed according to critical issues identif...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.08.032
更新日期：2018-01-01 00:00:00
abstract：:Technical challenges to record cortical unit spikes in conscious behavior monkeys have been presenting constraints in the investigation of cortical control of lower limb. Here we report a novel experimental system for investigation of correlation between cortical neuronal signals and standing and squatting movement. T...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.06.027
更新日期：2010-09-30 00:00:00
abstract：:The analgesic effects of various agents were evaluated in knee joint monoarthritic rats where arthritis was induced by monosodium urate (MSU) crystals injected into the knee joint cavities. The weight supported by each hind limb was measured quantitatively using pressure transducers, which revealed dose-dependent reco...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90026-c
更新日期：1990-08-01 00:00:00
abstract：:Alzheimer's disease (AD) is a progressive degenerative disorder of the brain characterized by a slow, progressive decline in cognitive function and behavior. As the disease advances, persons have a tough time with daily tasks like using the phone, cooking, handling money or driving the car. AD affects 15 million peopl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.10.036
更新日期：2009-03-15 00:00:00
abstract：:We describe a relational database (RDB) structure suitable for trial-based experiments such as human psychophysics and neural recording studies in trained animals. An RDB is a collection of tables, each composed of columns. Some of the tables contain columns that reference specific columns of other tables. This refere...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.05.014
更新日期：2005-01-30 00:00:00
abstract：:Voltage-sensitive dyes (VSDs) and optical imaging are useful for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes we were able to detect neuronal activity in single trials before it could be detected by local field potential (LFP) recordi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00511-8
更新日期：2002-03-30 00:00:00
abstract：:The neocortex is the site of origin of several forms of acquired epilepsy. Here we provide a brief review of experimental models that were recently developed to study neocortical epileptogenesis as well as some major results obtained with these methods. Most of neocortical seizures appear to be nocturnal and it is kno...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.08.030
更新日期：2016-02-15 00:00:00
abstract：BACKGROUND:Evaluation of motor deficits in rodents is mostly restricted to limb motor tests that are often high stressors for the animals. NEW METHOD:To test rodents for orofacial motor impairments in a stress-free environment, we established the pasta gnawing test by measuring the biting noise of mice that eat a piec...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.10.006
更新日期：2016-12-01 00:00:00
abstract：:Currently there is no reliable retrograde tracing technique for visualization of dendritic morphologies of projection neurons. Here we describe a simple and efficient method that can be used to label neurons in Golgi-like fashion. The approach relies on activity-dependent uptake of tracer. For this purpose we inject t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90055-v
更新日期：1993-10-01 00:00:00
abstract：:Previous research has shown that spatio-temporal waves in the EEG are generally of long spatial wavelength and form smooth patterns of phase gradients at particular time-samples. This paper describes a method to measure smooth phase gradients of long spatial wavelength in the EEG. The method depends on the global patt...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.02.016
更新日期：2006-09-30 00:00:00
abstract：:Incubation chambers for surviving brain slice preparations are in most cases designed to be stationary. For investigations on human brain tissue resected for the treatment of brain tumor or epilepsy, portable incubation chambers are needed in addition to stationary ones to allow transport of the slices between laborat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(96)00072-6
更新日期：1996-08-01 00:00:00
abstract：:A method is presented for the ultrastructural demonstration of succinate semialdehyde dehydrogenase (SSADH) activity in cerebellar Purkinje neurons and liver hepatocytes; SSADH is an enzyme involved in the degradation of gamma-aminobutyric acid (GABA). Incubation media originally used for light microscopy were conside...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90032-4
更新日期：1986-07-01 00:00:00
abstract：:Amyloid-beta (Abeta) deposits are one of the hallmarks of the neuropathological degeneration observed in Alzheimer's disease (AD) and Abeta concentrations have been reported to vary in different brain regions of AD patients. Abeta is produced by the sequential cleavage of amyloid precursor protein (APP) by beta-secret...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.05.001
更新日期：2006-10-30 00:00:00
abstract：:The Thy-1.2 expression cassette described in this report drives strong constitutive expression of transgenes specifically in the neurons of adult transgenic mice. Transgene expression begins at P6-10, at a time when it can affect activity-dependent rearrangements of synaptic connections and neuron-glia interactions du...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/s0165-0270(96)00121-5
更新日期：1997-01-01 00:00:00
abstract：:While it has long been recognized that humans recover function after stroke or head injury, the direct impact of drugs on recovery has only recently received attention. The ability of rats to traverse a narrow, elevated beam has proven to be particularly useful when studying the effects of pharmacologic manipulations ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90154-8
更新日期：1990-02-01 00:00:00
abstract：:Previous reports establishing raphe cultures typically yield less than 1% serotonin (5-HT)-positive neurons and are impractical for transcriptional studies. In this study, we have established primary cultures enriched in 5-HT neurons and quantified the proportion of cells expressing serotonergic and non-serotonergic m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.04.002
更新日期：2007-08-15 00:00:00
abstract：:The glycogen-depletion technique has become a well-established method for determining histologically the cross-sectional distribution of a single muscle unit. A major drawback of this method is its low yield of one depleted unit per experiment. Furthermore, this technique is particularly unsuited for determining the l...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90108-7
更新日期：1987-10-01 00:00:00
abstract：:A simple method which allows easy quantification of neuronal and glial sub-populations in primary neuronal tissue culture is described. The method takes advantage of the fact that the staining of neuronal and glia nuclei with Hoechst 33258 is distinctive and characteristic of the cell type. Double-staining experiments...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00207-w
更新日期：1995-07-01 00:00:00
abstract：:In order to understand the pathophysiology of temporal lobe epilepsy (TLE), and thus to develop new pharmacological treatments, in vivo animal models that present features similar to those seen in TLE patients have been developed during the last four decades. Some of these models are based on the systemic administrati...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.03.009
更新日期：2016-02-15 00:00:00
abstract：BACKGROUND:Rodents have been used for decades to probe neural circuits involved in behavior. Increasingly, attempts have been developed to standardize training paradigms across labs; and to use visual/auditory paradigms that can be also tested in humans. Commercially available systems are expensive and thus do not scal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108761
更新日期：2020-08-01 00:00:00
abstract：:Interactions among receptor neurons, glial cells and neurons intrinsic to the antennal lobe of the moth underlie the formation of olfactory glomeruli. To isolate these interactions, as well as to understand the effect of a variety of humoral agents on differentiation of the neurons and glia, we generate primary cultur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/S0165-0270(96)00025-8
更新日期：1996-10-21 00:00:00
abstract：:Background muscle activity (BGR) exerts a strong influence on stretch reflex amplitudes. While it is considered important to correct for this influence, which method best able to eliminate the effects of BGR remains unknown. We have therefore compared one previously described and one novel correction method which resp...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90152-h
更新日期：1993-02-01 00:00:00
