Abstract:
:Since no simple method for standardized positioning of very young rats in a stereotaxic device has been described in the literature, a new method for stereotaxic placement in the neonatal rat was developed, which only requires a simple body mould of the rat pup, and a minor adaptation of a rabbit stereotaxic apparatus (Kopf). The costs of the materials needed for construction of the neonatal rat body mould were approximately $1.-. Construction of the body mould took about one hour. Reliability and accuracy of the new method are demonstrated in a series of experiments in which the A10 group of dopaminergic cell bodies in the ventral tegmental area of 1-day-old rats were lesioned bilaterally: 15 out of 20 angular electrode placements resulted in lesions, the centres of which were located within the A10 group of dopaminergic cell bodies in the ventral tegmental area (max. 0.8 mm in diameter).
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Hoorneman EMdoi
10.1016/0165-0270(85)90122-0subject
Has Abstractpub_date
1985-07-01 00:00:00pages
109-16issue
2eissn
0165-0270issn
1872-678Xpii
0165-0270(85)90122-0journal_volume
14pub_type
杂志文章abstract::In spinal cord injury (SCI) research, axon regeneration across spinal lesions is most often assessed using anatomical methods. It would be extremely advantageous, however, to examine the functional synaptic connectivity of regenerating fibres, using high-resolution electrophysiological methods. We have therefore devel...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.06.017
更新日期:2011-09-15 00:00:00
abstract:BACKGROUND:Recently, hybrid brain-computer interfaces (BCIs) combining more than one modality have been investigated with the aim of boosting the performance of the existing single-modal BCIs in terms of accuracy and information transfer rate (ITR). Previously, we introduced a novel hybrid BCI in which EEG and fTCD mod...
journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90081-p
更新日期:1990-07-01 00:00:00
abstract::In this article, we describe an efficient method for testing both auditory receptor and auditory nerve function in a single recording session. Auditory receptor function is tested in response to pure tone, tone burst and click acoustic stimuli (i.e. distortion products of otoacoustic emissions, DPOAE; and auditory-evo...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2003.11.003
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abstract::Unlike certain other brain areas, notably the hippocampal formation, synaptic transmission in the cerebellum, a 3-dimensional anatomical structure, is no ideally studied in a 2-dimensional slice preparation. We describe a method for cutting a cerebellar slice that incorporates two planes of section: one sagittal (in t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00133-6
更新日期:1996-02-01 00:00:00
abstract::Practical aspects on the use of horseradish peroxidase (HRP) and wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) to trace peripheral cutaneous nerve endings have been studied. The parameters studied included application site of the tracer, post-application survival time, tracer concentration and trace...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90149-0
更新日期:1989-09-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.11.019
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abstract::Matrix metalloproteinases (MMPs) are implicated in the pathology of CNS tuberculosis. Whilst investigating the secretion of MMP-2 from human U373-MG astrocytoma cells, we observed elevated MMP-2 secretion in response to Middlebrook 7H9 media but not to Mycobacterium tuberculosis itself. Middlebrook 7H9 media did not s...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.06.007
更新日期:2008-08-30 00:00:00
abstract::We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We corr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90007-3
更新日期:1990-01-01 00:00:00
abstract::This report describes a digitally controlled system for use in auditory physiology in which signal generation and data acquisition are under the control of a single microcomputer. The system is designed to generate the complex waveforms required in neuroethological studies, and is able to generate these signals at fre...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(91)90136-n
更新日期:1991-01-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)00070-8
更新日期:1997-08-22 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.06.041
更新日期:2009-09-30 00:00:00
abstract:BACKGROUND:The neurological disorder known as epilepsy is characterized by involuntary recurrent seizures that diminish a patient's quality of life. Automatic seizure detection can help improve a patient's interaction with her/his environment, and while many approaches have been proposed the problem is still not trivia...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.03.024
更新日期:2016-06-15 00:00:00
abstract:BACKGROUND:The introduction of viral transneuronal tracers in the toolbox of neural tract-tracing methods has been an important addition in the field of connectomics for deciphering circuit-level architecture of the nervous system. One of the added values of viral compared to conventional retrograde tracers, in particu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
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更新日期:2020-10-01 00:00:00
abstract::We present a new analytical method for characterizing the directional tuning of neural data. The method is based on computing parameters associated with the geometric properties of solids, and provides an estimate of preferred direction in the context of non-uniform sampling of directions. Unlike optimization methods ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00492-7
更新日期:2002-01-30 00:00:00
abstract:BACKGROUND:Emotion recognition plays a key role in multimedia. To enhance the sensation of reality, smell has been incorporated into multimedia systems because it can directly stimulate memories and trigger strong emotions. NEW METHOD:For the recognition of olfactory-induced emotions, this study explored a combination...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108599
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abstract::Technical challenges to record cortical unit spikes in conscious behavior monkeys have been presenting constraints in the investigation of cortical control of lower limb. Here we report a novel experimental system for investigation of correlation between cortical neuronal signals and standing and squatting movement. T...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.06.027
更新日期:2010-09-30 00:00:00
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journal_title:Journal of neuroscience methods
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abstract:BACKGROUND:While several novel therapeutic approaches for HD are in development, resources to conduct clinical trials are limited. Large animal models have been proposed to improve assessment of safety, tolerability and especially to increase translational reliability of efficacy signals obtained in preclinical studies...
journal_title:Journal of neuroscience methods
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doi:10.1016/j.jneumeth.2015.11.013
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abstract::Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common genetic cause of Parkinson's disease. LRRK2 kinase activity is required for toxicity in neuronal cell cultures suggesting that selective kinase inhibitors may prevent neurodegeneration in patients. Directly monitoring LRRK2 activity in cells would b...
journal_title:Journal of neuroscience methods
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abstract::N-Acetylaspartate (NAA) is an important marker of neuronal function and viability that can be measured using magnetic resonance spectroscopy (MRS). In this paper, we proposed a method to measure NAA synthesis using proton MRS with infusion of uniformly (13)C-labeled glucose, and demonstrated its feasibility in an in v...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.04.001
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abstract::Monkeys were partially surrounded by opaque screens except for some distant small holes through which they observed various stimuli or actions, or well-defined background patterns. Gaze direction was measured by an infrared cornea reflection method. Without training or reward, the animal's gaze was directed through on...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.02.024
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.12.005
更新日期:2010-03-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)00114-3
更新日期:1997-11-07 00:00:00
abstract::An intra-arterially perfused working heart-brainstem preparation (WH-BP) was developed to allow studies into functionally identified cardiovascular and respiratory neurones in an in vitro milieu. This report provides that first description of this preparation. Evidence is presented indicating that the WH-BP: (1) spont...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00147-6
更新日期:1996-03-01 00:00:00
abstract::The detection and characterization of bursting activity remains a topic where no consensual definition has been reached so far. We compare here three different approaches of spike trains variability: statistical characterization (average frequency, coefficient of variation), burst detection (Poisson and rank surprise)...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:2003-06-30 00:00:00
abstract::Bone morphogenetic protein 7 (BMP7), a member of the transforming growth factor β (TGF-β) superfamily, plays important roles in the development of various tissues and organs in mouse and human. In particular, BMP7 is critical for the formation of the nervous system and it is considered to have therapeutic potential in...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.12.007
更新日期:2012-03-30 00:00:00