Abstract：
:The mitochondrial DNA (mtDNA) depletion status of rho(0) cell lines is typically assessed by hybridization or polymerase chain reaction (PCR) experiments, in which the failure to hybridize mtDNA or amplify mtDNA using mtDNA-directed primers suggests thorough mitochondrial genome removal. Here, we report the use of an mtDNA pseudogene ratioing technique for the additional confirmation of rho0 status. Total genomic DNA from a U251 human glioma cell line treated with ethidium bromide was amplified using primers designed to anneal either mtDNA or a previously described nuclear DNA-embedded mtDNA pseudogene (mtDNApsi). The resultant PCR product was used to generate plasmid clones. Sixty-two plasmid clones were genotyped, and all arose from mtDNApsi template. These data allowed us to determine with 95% confidence that the resultant mtDNA-depleted cell line contains less than one copy of mtDNA per 10 cells. Unlike previous hybridization or PCR-based analyses of mtDNA depletion, this mtDNApsi ratioing technique does not rely on interpretation of a negative result, and may prove useful as an adjunct for the determination of rho0 status or mtDNA copy number.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Swerdlow RH,Redpath GT,Binder DR,Davis JN 2nd,VandenBerg SRdoi
10.1016/j.jneumeth.2005.06.023subject
Has Abstractpub_date
2006-01-30 00:00:00pages
265-71issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(05)00221-9journal_volume
150pub_type
杂志文章abstract：:A new technique is described for accurate, reliable measurement of eyeball retraction in the rabbit. A narrow film strip, on which a linear light intensity grating has been exposed, is attached to a contact lens which is placed on the animal's cornea. The other end of the intensity grating slides between a photodiode ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90077-3
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND:Mixed frequency and phase coding (FPC) can achieve the significant increase of the number of commands in steady-state visual evoked potential-based brain-computer interface (SSVEP-BCI). However, the inconsistent phases of the SSVEP over channels in a trial and the existence of non-contributing channels due t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.04.001
更新日期：2018-07-01 00:00:00
abstract：UNLABELLED:Lobeline is a potential smoking cessation drug with affinity for the α4β2 nicotinic acetylcholine receptor and may inhibit the blood-brain barrier (BBB) amine transporter. The goal of this work was to use PET imaging to evaluate the effects of lobeline on the kinetic properties of [(18)F]nifene in the rat br...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.01.018
更新日期：2013-04-15 00:00:00
abstract：BACKGROUND:Mild traumatic brain injury (mTBI) strongly associates with chronic neurodegenerative impairments such as post-traumatic stress disorder (PTSD) and mild cognitive impairment. Early detection of concussive events would significantly enhance the understanding of head injuries and provide better guidance for ur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2021.109073
更新日期：2021-01-10 00:00:00
abstract：BACKGROUND:Machine learning models have been successfully applied to neuroimaging data to make predictions about behavioral and cognitive states of interest. While these multivariate methods have greatly advanced the field of neuroimaging, their application to electrophysiological data has been less common especially i...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.11.028
更新日期：2016-03-01 00:00:00
abstract：BACKGROUND:Supra-threshold galvanic vestibular stimulation (GVS) can be used to challenge the balance control system by disrupting vestibular inputs. The goal of this study was to propose an objective method to assess variability across subjects in the minimum safe GVS level that causes maximum balance degradation. New...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.10.012
更新日期：2019-01-01 00:00:00
abstract：:The Barnes maze is a visuo-spatial learning and memory test originally designed for use with rats, and later adapted for use with mice. The Barnes maze design and test procedure vary across studies using mice, but the effects of variation in Barnes maze design and test procedure on learning and memory in mice have not...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.09.027
更新日期：2012-01-30 00:00:00
abstract：:Antibody microprobes are devices which have been used to determine the release sites of several neuropeptides. The production of microprobes using the previous methodology is a time-consuming procedure requiring a high level of skill. The aim of the study was to investigate alternative methods for the production of mi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)90038-8
更新日期：1994-11-01 00:00:00
abstract：:Selective and simultaneous voltammetric analysis of catechols and indoles in vivo and in vitro has until now been feasible only by means of 'slow' scanning methods (scan speed in tens of seconds) such as differential pulse (DPV) and differential normal pulse voltammetry in conjunction with electrically and/or chemical...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00043-g
更新日期：1995-09-01 00:00:00
abstract：BACKGROUND:Primary astrocyte cultures have been used for decades to study astrocyte functions in health and disease. The current primary astrocyte cultures are mostly maintained in serum-containing medium which produces astrocytes with a reactive phenotype as compared to in vivo quiescent astrocytes. The aim of this st...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.03.013
更新日期：2019-05-15 00:00:00
abstract：BACKGROUND:Providing weight support facilitates locomotion in spinal cord injured animals. To control weight support, robotic systems have been developed for treadmill stepping and more recently for overground walking. NEW METHOD:We developed a novel device, the body weight supported ambulatory rodent trainer (i.e. BA...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.015
更新日期：2015-05-15 00:00:00
abstract：BACKGROUND:Tree shrews are proposed as an alternative animal model to nonhuman primates due to their close affinity to primates. Neuroimaging techniques are widely used to study brain functions and structures of humans and animals. However, tree shrews are rarely applied in neuroimaging field partly due to the lack of ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.09.006
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Contralateral seventh cervical nerve transfer (contralateral C7 transfer) is a novel treatment for patients with spastic paralysis, including stroke and traumatic brain injury. However, little is known on changes in plasticity that occur in the intact hemisphere after C7 transfer. An appropriate surgical mod...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108445
更新日期：2019-12-01 00:00:00
abstract：BACKGROUND:Assessing markers of neural activity in rodent behavioural tasks benefits from meaningful comparison with matched control conditions. The Morris water maze is a widely used learning and memory task, but currently implemented control conditions are not optimal. NEW METHOD:We created a novel control condition...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.02.015
更新日期：2019-04-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.01.009
更新日期：2015-03-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.01.007
更新日期：2009-04-30 00:00:00
abstract：BACKGROUND:Image segmentation is often imperfect, particularly in complex image sets such z-stack micrographs of slice cultures and there is a need for sufficient details of parameters used in quantitative image analysis to allow independent repeatability and appraisal. NEW METHOD:For the first time, we have criticall...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.12.002
更新日期：2018-02-01 00:00:00
abstract：BACKGROUND:Transcranial magnetic stimulation (TMS) can be paired with functional magnetic resonance imaging (fMRI) in concurrent TMS-fMRI experiments. These multimodal experiments enable causal probing of network architecture in the human brain which can complement alternative network mapping approaches. Critically, me...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108400
更新日期：2019-11-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00164-8
更新日期：2000-02-15 00:00:00
abstract：:Using a specific rat monoclonal anti-idiotypic antibody we have examined the subcellular distribution of -opioid receptors in various neuronal subtypes of the rat spinal cord. The immunofluorescence was detected with a confocal microscope and in some cases serial images were processed for a three-dimensional (3-D) rec...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00149-6
更新日期：1999-02-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：1986-09-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.12.018
更新日期：2011-02-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(81)90045-5
更新日期：1981-08-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00068-2
更新日期：2003-06-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90040-x
更新日期：1989-06-01 00:00:00
abstract：BACKGROUND:Given the movement of molecules within tissue that occurs naturally by endogenous electric fields, we examined the possibility of using a low-voltage DC field to move charged substances in rodent peripheral nerve in vitro. NEW METHOD:Labeled sugar- and protein-based markers were applied to a rodent peroneal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.01.018
更新日期：2014-03-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.10.023
更新日期：2012-02-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90056-8
更新日期：1988-06-01 00:00:00
abstract：:The present study demonstrates that bipotential neural precursors isolated from an early developmental stage of the sheep embryo nervous system can be maintained in vitro in an undifferentiated state for a long period. These precursors multiplied under the action of epidermal growth factor and basic fibroblast growth ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00363-6
更新日期：2001-05-30 00:00:00
abstract：:Diffusion-weighted MRI is the forerunner of the rapidly developed microstructure MRI (μMRI) aimed at in vivo evaluation of the cellular tissue architecture. This brief review focuses on the spatiotemporal scales of the microstructure that are accessible using different diffusion MRI techniques and the need to weight t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2020.108910
更新日期：2021-01-01 00:00:00
