Abstract:
:A reliable and economical silver staining method is recommended for demonstration of Lewy bodies (LB) and Lewy neurites (LN) in Parkinson's disease (PD) and of argyrophilic oligodendrocytes in multiple system atrophy (MSA). The technique can be applied to routinely formalin-fixed autopsy material and does not require particular skills. It permits processing of frozen sections and sections from polyethylene glycol or paraffin embedded material. It takes advantage of the physical development of nucleation sites and thereby permits tight control of the entire staining procedure.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Braak E,Braak Hdoi
10.1016/s0165-0270(98)00173-3subject
Has Abstractpub_date
1999-02-01 00:00:00pages
111-5issue
1eissn
0165-0270issn
1872-678Xpii
S0165027098001733journal_volume
87pub_type
杂志文章abstract::This study demonstrated that liposome-mediated transfection - lipofection - is suitable for delivering genes into astrocytes. By repeatedly lipofecting the same astrocyte cultures, a process we call multi-lipofection, the transfection efficiency of the beta-galactosidase (beta-gal) gene was improved from 2.6+/-0.6 to ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00285-5
更新日期:2000-10-30 00:00:00
abstract:BACKGROUND:Windowed Fourier decompositions (WFD) are widely used in measuring stationary and non-stationary spectral phenomena and in describing pairwise relationships among multiple signals. Although a variety of WFDs see frequent application in electrophysiological research, including the short-time Fourier transform...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.12.004
更新日期:2016-03-01 00:00:00
abstract::The Barnes maze is a visuo-spatial learning and memory test originally designed for use with rats, and later adapted for use with mice. The Barnes maze design and test procedure vary across studies using mice, but the effects of variation in Barnes maze design and test procedure on learning and memory in mice have not...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.09.027
更新日期:2012-01-30 00:00:00
abstract::We have attempted to demonstrate the technical requirements and performance of a microscopical assay using a densitometric application of image analysis to measure immunohistochemical stain intensity. Not surprisingly, the techniques required were more demanding than those used for the quantification of field and obje...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(92)90048-i
更新日期:1992-10-01 00:00:00
abstract::Functionally relevant large scale brain dynamics operates within the framework imposed by anatomical connectivity and time delays due to finite transmission speeds. To gain insight on the reliability and comparability of large scale brain network simulations, we investigate the effects of variations in the anatomical ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.07.007
更新日期:2009-09-30 00:00:00
abstract::The most common behavioural disorder seen in children and adolescents born extremely prematurely is attention deficit hyperactivity disorder (ADHD). The hyperactive/impulsive sub-type of ADHD or the inattentive sub-type or the hyperactive/impulsive/inattentive sub-type can be evident. These sub-types of ADHD can persi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.01.010
更新日期:2007-11-30 00:00:00
abstract:BACKGROUND:Recently, hybrid brain-computer interfaces (BCIs) combining more than one modality have been investigated with the aim of boosting the performance of the existing single-modal BCIs in terms of accuracy and information transfer rate (ITR). Previously, we introduced a novel hybrid BCI in which EEG and fTCD mod...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.03.018
更新日期:2019-05-15 00:00:00
abstract::The last decade has seen a surge in the study of nonlinear dynamical behavior in physiologic systems. In this paper, some of the computational techniques most commonly used to investigate the nonlinear dynamics of these systems are described. Applications to eye movement analysis are included, including the validation...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/s0165-0270(98)00062-4
更新日期:1998-08-31 00:00:00
abstract::The brain is a complex neural network with interleaving functional connectivity among anatomical regions. However, current functional parcellation algorithms usually emphasize independence or orthogonality between the spatial components, with the interleaving nature underrepresented. This study investigates a method, ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.05.001
更新日期:2011-07-15 00:00:00
abstract:BACKGROUND:MRI-histology correlation studies of the ex vivo brain mostly employ fresh, extracted (ex situ) specimens, aldehyde fixed by immersion, which has several disadvantages for MRI scanning (e.g. deformation of the organ). A minority of studies are done ex vivo-in situ (unfixed brain), requiring an MRI scanner re...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108903
更新日期:2020-11-01 00:00:00
abstract::Electroneurograms (ENGs) from the vagus, splanchnic and pancreatic nerves innervating the pancreas of a dog, were recorded with chronically implanted silicone multi-electrode circular cuffs in an intact pancreas and in a pancreas partly disabled with alloxan. The cuffs contained 33 platinum electrodes (0.6x1.5 mm) arr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00462-9
更新日期:2001-12-15 00:00:00
abstract:BACKGROUND:Glucose hypermetabolism in denervated skeletal muscle suggests the potential for developing a diagnostic tool for peripheral nerve injuries. Herein, we investigated the characteristics and molecular mechanism of this phenomenon. NEW METHOD:Temporal course of glucose hypermetabolism and development of abnorm...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.01.014
更新日期:2019-04-01 00:00:00
abstract::Functional magnetic resonance imaging allows precise localization of brain regions specialized for different perceptual and higher cognitive functions. However, targeting these deep brain structures for electrophysiology still remains a challenging task. Here, we propose a novel framework for MRI-stereotactic registra...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.11.031
更新日期:2012-03-15 00:00:00
abstract:BACKGROUND:Functional magnetic resonance imaging (fMRI) enables non-invasive examination of both the structure and the function of the human brain. The prevalence of high spatial-resolution (sub-millimeter) fMRI has triggered new research on the intra-cortex, such as cortical columns and cortical layers. At present, ec...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108625
更新日期:2020-04-15 00:00:00
abstract::This review considers the different forms of reversible deactivation and how they differ from each other and from more conventional permanent deactivation methods. The different methods of chemical and cooling reversible inactivation and the limitations of each are discussed. Finally, future directions in the quest to...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/s0165-0270(98)00160-5
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:Although they form a unitary phenomenon, the relationship between extracranial M/EEG and transmembrane ion flows is understood only as a general principle rather than as a well-articulated and quantified causal chain. METHOD:We present an integrated multiscale model, consisting of a neural simulation of tha...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.10.002
更新日期:2019-03-15 00:00:00
abstract::The fluorescent dye Fluorogold has been recently introduced as a retrograde neuronal tracer that can be used in combination with immunohistochemistry. It has, however, lately been reported that immunohistochemical processes induced rapid photobleaching of Fluorogold. In the present study we prove that it is the water ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90109-x
更新日期:1989-07-01 00:00:00
abstract::Microglia and astrocytes play vital roles in normal human brain function and in neurological disorders. To study their physiological and pathological roles it is desirable to establish in vitro systems that are derived from the adult human brain. Although several groups have successfully cultured cells from the human ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.07.005
更新日期:2007-10-15 00:00:00
abstract::The physical basis for electrical stimulation of excitable tissue, as used by electrophysiological researchers and clinicians in functional electrical stimulation, is presented with emphasis on the fundamental mechanisms of charge injection at the electrode/tissue interface. Faradaic and non-Faradaic charge transfer m...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2004.10.020
更新日期:2005-02-15 00:00:00
abstract::We investigated the properties of biotinylated dextran-amine (BDA) as a neuroanatomical tracer at the electron microscopic level and we compared the results with those obtained previously with another tracer, the lectin Phaseolus vulgaris-leucoagglutinin (PHA-L). BDA was injected into various brain areas of rats. Foll...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(05)80009-3
更新日期:1993-06-01 00:00:00
abstract::After injection of 2-deoxyglucose (2-DG) labeled with tritium or carbon-14, autoradiograms were produced by thaw-mounting 4 micron frozen sections of rat brains on nuclear emulsion-coated slides. The results show that the distribution of radioactivity among different brain regions was similar and that the resolution a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(87)90043-4
更新日期:1987-06-01 00:00:00
abstract::The implantation of a foreign object in the brain produces an acute neuroinflammatory state in which glia (astrocytes and microglia) may remain chronically activated in response to the inert foreign object. Activated glia can exhibit a sensitized pro-inflammatory response to immunogenic stimuli. This may be relevant t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.11.014
更新日期:2007-04-15 00:00:00
abstract::The silanization of glass, particularly Pyrex, was studied using reaction conditions that might be applied in the fabrication of ion-sensitive microelectrodes of the liquid-membrane type. The efficacy was tested by measuring the hydrophobicity (contact angle) or electrical resistivity of the treated surface. Aminosila...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(83)90037-7
更新日期:1983-07-01 00:00:00
abstract::A new video path analyzer, described herein, monitors travel distance, rearing, stereotypic movement, rotation, and speed of rats simultaneously. The video path analyzer was equipped with a cage (black background, 50 cm long x 50 cm wide x 50 cm high), camera, television screen, computer, and printer. A detailed descr...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/S0165-0270(96)00101-X
更新日期:1996-12-01 00:00:00
abstract::A low-noise flexible system for the simultaneous recording and analysis of several electrical signals (EEG, ECG, EMG, and diaphragm EMG) from the same rat was constructed for studying changes in physiological functions during the sleep-wake cycle. The hardware in the system includes a multichannel amplifier, a video c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00146-2
更新日期:2002-07-30 00:00:00
abstract::Depression is characterized by a lack of "motivation" rather than a lack of "physical space" to move around. This study was designed to evaluate predictivity of an open space swimming test for antidepressant activity of various antidepressants in rats. Without drug treatment, rats showed a significant reduction in the...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(03)00068-2
更新日期:2003-06-15 00:00:00
abstract::The present study concerns an ELISA for glial fibrillary acidic protein (GFAP) in cerebrospinal fluid (CSF). The application of the method in CSF of children has previously been described in this journal. We have now adapted the technique to allow determination of the much higher GFAP concentrations found in normal an...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)90011-6
更新日期:1994-03-01 00:00:00
abstract::Adult rat spinal cords were perfused intracardially with aldehyde fixatives containing either sodium cacodylate or sodium phosphate buffer for EM studies. The perfusion flow rate of 24 cc/min for the first 3 min and 33 cc/min for completion of prefixation was found to be optimal. Ultrastructural examination of the L-1...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(82)90047-4
更新日期:1982-01-01 00:00:00
abstract:BACKGROUND:Large animal models of human neurological disorders are advantageous compared to rodent models due to their neuroanatomical complexity, longevity and their ability to be maintained in naturalised environments. Some large animal models spontaneously develop behaviours that closely resemble the symptoms of neu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.01.018
更新日期:2017-12-01 00:00:00
abstract::There are many reasons why novel therapeutics fail in clinical trials but these failures are often attributed to lacking quality of preclinical data. These problems are not limited to any specific therapeutic area, academic or industrial research and are due in large part to several generic factors influencing researc...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.10.018
更新日期:2018-04-15 00:00:00