Abstract：
:The thalamocortical slice is widely employed for in vitro studies of cortical circuits. This preparation was developed in order to preserve anatomical and functional connectivity between the ventrobasal thalamus and somatosensory (whisker/barrel) cortex of young mice, and thalamocortical slice experiments have contributed significantly to our understanding of the thalamocortical synapse. Cortical somatotopy within thalamocortical slices, however, has not been characterized, and this greatly limits their use in studies that require identification of cortical areas associated with particular regions of the sensory periphery. To address this shortcoming we used electrophysiological recording and neuroanatomical labeling techniques in rats to mark the position of functionally defined whisker barrels, in vivo. We subsequently processed the brains in a plane appropriate for TC slices and characterized the location of somatotopically identified barrels in relation to other aspects of slice topology. We found that barrels associated with the large mobile whiskers occupy a particular location in TC slices, but that there are certain constraints to studying this portion of the barrelfield in vitro.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Land PW,Kandler Kdoi
10.1016/s0165-0270(02)00150-4subject
Has Abstractpub_date
2002-09-15 00:00:00pages
15-21issue
1eissn
0165-0270issn
1872-678Xpii
S0165027002001504journal_volume
119pub_type
杂志文章abstract：:Transcranial Electrical Stimulation (tES) encompasses all methods of non-invasive current application to the brain used in research and clinical practice. We present the first comprehensive and technical review, explaining the evolution of tES in both terminology and dosage over the past 100 years of research to prese...
journal_title：Journal of neuroscience methods
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.jneumeth.2013.07.016
更新日期：2013-10-15 00:00:00
abstract：:The present work introduces an electrode microdrive system small enough to be placed into two distinct brain areas in head-fixed mice. To meet the space constraint imposed by the size of mice and the additional presence of a head post, the size and weight of the components were minimized. In the current version, an in...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.12.015
更新日期：2010-03-15 00:00:00
abstract：:A new method to study the reactions to unexpected mechanical perturbations during human walking on a treadmill is presented. Perturbations consisted of an obstruction of the forward swinging foot during the early swing phase. These were caused by obstacles which were dropped on the treadmill in front of the subject. T...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00149-2
更新日期：1996-07-01 00:00:00
abstract：:Stress has long been known to affect grooming in rodent species, altering both its activity measures and behavioural microstructure. Since stress disturbs a general pattern of self-grooming uninterrupted cephalocaudal progression, the grooming analysis algorithm (Kalueff and Tuohimaa, Brain Res. Protocols, 2004; 15: 1...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.10.001
更新日期：2005-04-30 00:00:00
abstract：:The objective of visual systems neuroscience has shifted over the past few years from determining the receptive fields of cells towards the understanding of higher level cognition in awake animals viewing natural stimuli. In experiments with awake animals it is important to control the relevant aspects of behavior. Mo...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00423-x
更新日期：2001-09-30 00:00:00
abstract：:Implantable microfabricated microelectrode arrays represent a versatile and powerful tool to record electrophysiological activity across multiple spatial locations in the brain. Spikes and field potentials, however, correspond to only a fraction of the physiological information available at the neural interface. In ur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.06.036
更新日期：2008-09-15 00:00:00
abstract：:Experimental animal epilepsy research got a big boost since the discovery that daily mild and short (seconds) tetanic stimulations in selected brain regions led to seizures with increasing duration and severity. This model that was developed by Goddard (1967) became known as the kindling model for epileptogenesis and ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.03.025
更新日期：2016-02-15 00:00:00
abstract：:The amplitude of the maximal direct motor response (Mmax) elicited by supramaximal peripheral nerve stimulation can vary with time and with changes in muscle length. We sought to investigate the variability in the amplitude of Mmax in the human wrist flexors (flexor carpi radialis, FCR) at a constant joint position du...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.03.016
更新日期：2005-12-15 00:00:00
abstract：BACKGROUND:Zebrafish larvae have a high potential as model system to replace rodents, especially in screening and drug discovery applications. However, an experimental setup to deliver mild electrical stimuli with simultaneous high throughput behavioural tracking has not yet been described. NEW METHOD:A new tool was d...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.03.002
更新日期：2018-05-01 00:00:00
abstract：:Extracellular electrical stimulation is increasingly used for in vitro neural experimentation, including brain slices and cultured cells. Although it is desirable to record directly from the stimulating electrode, relatively high stimulation levels make it extremely difficult to record immediately after the stimulatio...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.11.017
更新日期：2009-03-30 00:00:00
abstract：:We have developed a labeling procedure which accurately and consistently labels the original sensory pools projecting to their respective nerve branches as a model to quantify the accuracy of nerve regeneration at the single neuron level. Adult and juvenile rats had the saphenous branch of the femoral nerve transected...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90078-e
更新日期：1991-09-01 00:00:00
abstract：:Griffanti et al. (2017) provide a rich set of expert-consensus guidelines for how to label components derived from ICA as either belonging to signal of interest or noise to be filtered from fMRI data. These general hypotheses concerning procedures for "hand" classification of components are a good starting point that ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.07.007
更新日期：2017-10-01 00:00:00
abstract：BACKGROUND:Functional Magnetic Resonance Imaging (fMRI) is used for exploring brain functionality, and recently it was applied for mapping the brain connection patterns. To give a meaningful neurobiological interpretation to the connectivity network, it is fundamental to properly define the network framework. In partic...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.03.004
更新日期：2014-05-15 00:00:00
abstract：:Typically small animal radiological images are obtained after placing the animal in the center of the imaging device using beds or platforms, and then adjusting the position after obtaining a scout image. Such a process does not permit the reproducible visualization of the same anatomical plane with repeated examinati...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.01.003
更新日期：2004-06-15 00:00:00
abstract：:We hypothesized that re-entry into the cell cycle may be associated with reactive gliosis surrounding neural prostheses, and that administration of a cell cycle inhibitor (flavopiridol) at the time of surgery would reduce this effect. We investigated the effects of flavopiridol on recording quality and impedance over ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.026
更新日期：2009-10-15 00:00:00
abstract：BACKGROUND:Multielectrode arrays (MEAs) have been used to understand electrophysiological network dynamics by recording real-time activity in groups of cells. The extent to which the collection of such data enables hypothesis testing on the level of circuits and networks depends largely on the sophistication of the ana...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.02.011
更新日期：2016-05-01 00:00:00
abstract：:Measurements of neurotransmitters in conscious animals have been restrictive in real-time. The use of specific enzymes within an amperometric probe, based upon a microdialysis membrane, may overcome some of these problems. I report the use of such a probe, with different perfusions of enzymes, to allow real-time measu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)02175-9
更新日期：1997-04-04 00:00:00
abstract：:Sensory neurons encode external information by a series of times of action potentials, which is called a spike train. However, since it is a point process, it is hard to analyze. Here we propose a method for converting a spike train into a real-valued time series with a fixed sampling interval under the assumption of ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.030
更新日期：2009-10-15 00:00:00
abstract：:Analysis of the antigenic components of synaptic membranes presents several problems caused by detergents required to solubilize the proteins. These problems involve detection of the reaction between antibodies and proteins in the detergent extract as well as characterization of the individual antigens after further p...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90058-8
更新日期：1980-04-01 00:00:00
abstract：BACKGROUND:Transcranial magnetic stimulation (TMS) can evaluate cortical excitability and integrity of motor pathways via TMS-induced responses. The responses are affected by the orientation of the stimulated neurons with respect to the direction of the TMS-induced electric field. Therefore, besides being a functional ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.08.028
更新日期：2015-12-30 00:00:00
abstract：:The chronic intrathecal catheterization of the subarachnoid space in the rat is a very useful model in the research of neuroscience, anesthesiology, neurotoxicity, and etc. To avoid high postoperative mortality and neurological impairment, the catheterization from the lumbar interspace has been widely used in recent y...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.020
更新日期：2009-10-15 00:00:00
abstract：:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90045-3
更新日期：1988-01-01 00:00:00
abstract：:An improved method using HRP for visualizing axons in whole-mount preparations is described. Application of this method to preparations of regenerating frog peripheral nerves maintained in organ culture was used to show that regeneration following nerve crush begins after about 3 days. ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90110-y
更新日期：1992-08-01 00:00:00
abstract：:The interactions among cerebral regions involved in semantic word generations are explored through connectivity analysis based on fMRI data through multivariate autoregressive model (MVAR). Connections among the pars triangularis of the left inferior frontal gyrus (L45), the lateral fusiform girus (LFG) and the left m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.10.005
更新日期：2013-01-30 00:00:00
abstract：:There are many reasons why novel therapeutics fail in clinical trials but these failures are often attributed to lacking quality of preclinical data. These problems are not limited to any specific therapeutic area, academic or industrial research and are due in large part to several generic factors influencing researc...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.10.018
更新日期：2018-04-15 00:00:00
abstract：BACKGROUND:Alzheimer's disease (AD) is the most common cause of neurodegenerative dementia in the elderly population. Scientific research is very active in the challenge of designing automated approaches to achieve an early and certain diagnosis. Recently an international competition among AD predictors has been organi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.11.002
更新日期：2018-05-15 00:00:00
abstract：BACKGROUND:A key point in developmental biology is to understand how gene expression influences the morphological and dynamical patterns that are observed in living beings. NEW METHOD:In this work we propose a methodology capable of addressing this problem that is based on estimating the mutual information and Pearson...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.02.019
更新日期：2015-04-30 00:00:00
abstract：BACKGROUND:Neurite outgrowth is a metric widely used to assess the success of in vitro neural stem cell differentiation or neuron reprogramming protocols and to evaluate high-content screening assays for neural regenerative drug discovery. However, neurite measurements are tedious to perform manually, and there is a pa...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.03.013
更新日期：2017-05-01 00:00:00
abstract：BACKGROUND:Putative treatments derived from in vivo stem cell transplant-derived dopamine (DA) in hemiparkinsonian rats have been assessed via DA-agonist-induced rotations involving imbalanced intra-hemispheric striatal DA receptor stimulation. However, such tests obscure the natural responses of grafts to sensory stim...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.06.005
更新日期：2018-09-01 00:00:00
abstract：:Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of ischaemic stroke. In particular, the mature forms of MMPs 2 and 9 have similar sizes and share gelatine as a common substrate. Both MMPs are upregulated in ischaemic stroke and play detrimental roles during stroke pathogenesis. Throughout t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.03.007
更新日期：2013-05-30 00:00:00
