Abstract：
BACKGROUND:Putative treatments derived from in vivo stem cell transplant-derived dopamine (DA) in hemiparkinsonian rats have been assessed via DA-agonist-induced rotations involving imbalanced intra-hemispheric striatal DA receptor stimulation. However, such tests obscure the natural responses of grafts to sensory stimuli, and drug-induced plasticity can modify the circuit being tested. Thus, we propose an alternative testing strategy using a novel water tank swimming apparatus. NEW METHOD:Microdialysis was used to compare striatal DA levels when rats were: (1) in a rest-phase within a bowl-shaped apparatus, or (2) in an active forced-swim phase within a specially-equipped water tank. Resting-phase DA release levels were compared with active-phase levels obtained while rats were required to swim in the water-tank task. Behavioral variables such as asymmetric circling while swimming (rotations), front-limb strokes, and front-limb reaches were captured by a camera for analysis. RESULTS AND COMPARISON WITH EXISTING METHODS:Transplanted cells had a very modest effect on percentage of contralateral front-limb strokes, but did not reduce lesion-induced rotational asymmetry in the swim task. Neither striatal DA levels, nor their breakdown products, were significantly different between transplanted and sham-transplanted groups. Our new behavioral test eliminates the need for pharmacological stimulation, enabling simultaneous assessment of DA released in resting and active phases to explore graft control. CONCLUSIONS:Our new method allows for accurate assessments of stem cell therapy for PD as an alternative to "rotation" tests. Use of natural motivations to engage in sensory-driven motor tasks provides more accurate insights into ongoing graft-derived behavioral support.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Bhupal PK,Anderson KA,Shall GP,Lynn JD,Hoolsema KS,Rossignol J,Dunbar GL,Sandstrom MIdoi
10.1016/j.jneumeth.2018.06.005subject
Has Abstractpub_date
2018-09-01 00:00:00pages
149-163eissn
0165-0270issn
1872-678Xpii
S0165-0270(18)30177-8journal_volume
307pub_type
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