Abstract:
:In vitro and in vivo models of experimental glioma are useful tools to gain a better understanding of glioblastoma (GBM) and to investigate novel treatment strategies. However, the majority of preclinical models focus on treating solid intracranial tumours, despite surgical resection being the mainstay in the standard care of patients with GBM today. The lack of resection and recurrence models therefore has undermined efforts in finding a treatment for this disease. Here we present a novel orthotopic tumour resection and recurrence model that has potential for the investigation of local delivery strategies in the treatment of GBM. The model presented is simple to achieve through the use of a biopsy punch, is reproducible, does not require specific or expensive equipment, and results in a resection cavity suitable for local drug delivery systems, such as the implantation or injection of hydrogels. We show that tumour resection is well tolerated, does not induce deleterious neurological deficits, and significantly prolongs survival of mice bearing U-87 MG GBM tumours. In addition, the resulting cavity could accommodate adequate amounts of hydrogels for local delivery of chemotherapeutic agents to eliminate residual tumour cells that can induce tumour recurrence.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Bianco J,Bastiancich C,Joudiou N,Gallez B,des Rieux A,Danhier Fdoi
10.1016/j.jneumeth.2017.04.019subject
Has Abstractpub_date
2017-06-01 00:00:00pages
96-102eissn
0165-0270issn
1872-678Xpii
S0165-0270(17)30118-8journal_volume
284pub_type
杂志文章abstract:BACKGROUND:Artificial neural networks (ANNs) are one of the widely used classifiers in the brain-computer interface (BCI) systems-based on noninvasive electroencephalography (EEG) signals. Among the different ANN architectures, the most commonly applied for BCI classifiers is the multilayer perceptron (MLP). When appro...
journal_title:Journal of neuroscience methods
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更新日期:2018-07-15 00:00:00
abstract::While it has long been recognized that humans recover function after stroke or head injury, the direct impact of drugs on recovery has only recently received attention. The ability of rats to traverse a narrow, elevated beam has proven to be particularly useful when studying the effects of pharmacologic manipulations ...
journal_title:Journal of neuroscience methods
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doi:10.1016/0165-0270(90)90154-8
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abstract::Exponential decay of the power spectrum is typically observed in turbulent flow patterns, as evidenced both in experiments and in the Navier-Stokes equations describing fluid dynamics. In this study, we present evidence on the exponential decay of power spectra belonging to interspike interval time series, registered ...
journal_title:Journal of neuroscience methods
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abstract::Recombinant proteins are routinely expressed in heterologous expression systems such as human embryonic kidney 293 (HEK 293) cells. The efficiency of the expression is critical when the expressed protein must be characterized at the single-cell level. Here we describe a simple method by which the protein expression ef...
journal_title:Journal of neuroscience methods
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abstract::The glycogen-depletion technique has become a well-established method for determining histologically the cross-sectional distribution of a single muscle unit. A major drawback of this method is its low yield of one depleted unit per experiment. Furthermore, this technique is particularly unsuited for determining the l...
journal_title:Journal of neuroscience methods
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abstract:BACKGROUND:The neurological disorder known as epilepsy is characterized by involuntary recurrent seizures that diminish a patient's quality of life. Automatic seizure detection can help improve a patient's interaction with her/his environment, and while many approaches have been proposed the problem is still not trivia...
journal_title:Journal of neuroscience methods
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abstract:BACKGROUND:Over 90 years after its first recording, scalp electroencephalography (EEG) remains one of the most widely used techniques in human neuroscience research, in particular for the study of event-related potentials (ERPs). However, because of its low signal-to-noise ratio, extracting useful information from thes...
journal_title:Journal of neuroscience methods
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doi:10.1016/j.jneumeth.2017.12.014
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abstract::In order to understand the pathophysiology of temporal lobe epilepsy (TLE), and thus to develop new pharmacological treatments, in vivo animal models that present features similar to those seen in TLE patients have been developed during the last four decades. Some of these models are based on the systemic administrati...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2015.03.009
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abstract::The present work introduces an electrode microdrive system small enough to be placed into two distinct brain areas in head-fixed mice. To meet the space constraint imposed by the size of mice and the additional presence of a head post, the size and weight of the components were minimized. In the current version, an in...
journal_title:Journal of neuroscience methods
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doi:10.1016/j.jneumeth.2009.12.015
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abstract::Analysis of cortical patterns requires accurate cortical surface registration. Many researchers map the cortical surface onto a unit sphere and perform registration of two images defined on the unit sphere. Here we have developed a novel registration framework for the cortical surface based on spherical thin-plate spl...
journal_title:Journal of neuroscience methods
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doi:10.1016/j.jneumeth.2012.02.010
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.02.016
更新日期:2006-09-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.12.005
更新日期:2015-02-15 00:00:00
abstract:BACKGROUND:Tree shrews are proposed as an alternative animal model to nonhuman primates due to their close affinity to primates. Neuroimaging techniques are widely used to study brain functions and structures of humans and animals. However, tree shrews are rarely applied in neuroimaging field partly due to the lack of ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.09.006
更新日期:2018-01-01 00:00:00
abstract::Currently there is no reliable retrograde tracing technique for visualization of dendritic morphologies of projection neurons. Here we describe a simple and efficient method that can be used to label neurons in Golgi-like fashion. The approach relies on activity-dependent uptake of tracer. For this purpose we inject t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90055-v
更新日期:1993-10-01 00:00:00
abstract::A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in ...
journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00222-3
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abstract::Microdialysis coupled to liquid chromatography (LC) has proven to be a valuable in vivo sampling technique for studying neurotransmitter changes in normal and ischaemic brain. However, few analytical methods have described the simultaneous determination of amino acids, relevant in stroke research, together with the ni...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.10.013
更新日期:2005-05-15 00:00:00
abstract::The naked mole-rat (Heterocephalus glaber) is a unique fossorial mammal that is eusocial, object blind, and virtually poikilothermic. This animal is an ideal model to examine questions pertaining to the thermoregulatory aspects of sleep/wake cycles and other circadian events. However, the monitoring of mammalian sleep...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.04.014
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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doi:10.1016/j.jneumeth.2015.07.018
更新日期:2015-10-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.12.026
更新日期:2018-03-01 00:00:00
abstract::The quantification of synapses in neuronal cultures is essential in studies of the molecular mechanisms underlying synaptogenesis and synaptic plasticity. Conventional counting of synapses based on morphological or immunocytochemical criteria is extremely work-intensive. We developed a fully automated method which qua...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.11.010
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abstract::Diffusion-weighted MRI is the forerunner of the rapidly developed microstructure MRI (μMRI) aimed at in vivo evaluation of the cellular tissue architecture. This brief review focuses on the spatiotemporal scales of the microstructure that are accessible using different diffusion MRI techniques and the need to weight t...
journal_title:Journal of neuroscience methods
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abstract::Accurate estimates of the total number of neurons located in the wall of the gut are essential for studies of the enteric nervous system (ENS). Though several stains and antibodies are used routinely as pan-neuronal markers, controversies of relative sensitivity and completeness have been difficult to resolve, at leas...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:2004-02-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(86)90043-9
更新日期:1986-04-01 00:00:00