Animal models of temporal lobe epilepsy following systemic chemoconvulsant administration.


:In order to understand the pathophysiology of temporal lobe epilepsy (TLE), and thus to develop new pharmacological treatments, in vivo animal models that present features similar to those seen in TLE patients have been developed during the last four decades. Some of these models are based on the systemic administration of chemoconvulsants to induce an initial precipitating injury (status epilepticus) that is followed by the appearance of recurrent seizures originating from limbic structures. In this paper we will review two chemically-induced TLE models, namely the kainic acid and pilocarpine models, which have been widely employed in basic epilepsy research. Specifically, we will take into consideration their behavioral, electroencephalographic and neuropathologic features. We will also evaluate the response of these models to anti-epileptic drugs and the impact they might have in developing new treatments for TLE.


J Neurosci Methods


Lévesque M,Avoli M,Bernard C




Has Abstract


2016-02-15 00:00:00












  • An automated system for measuring tip impedance and among-electrode shunting in high-electrode count microelectrode arrays.

    abstract::The development and effective use of robust high-electrode-count microelectrode arrays for neuronal recording and stimulation depends on effective monitoring of electrode impedances and how these change over time. In multielectrode arrays, conventional electrode impedance measurements may be confounded by possible shu...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Gunalan K,Warren DJ,Perry JD,Normann RA,Clark GA

    更新日期:2009-04-15 00:00:00

  • Computed detection and quantitative morphometry of Alzheimer senile plaques.

    abstract::Senile plaques (SP) are the most characteristic neuropathologic lesions of Alzheimer's disease (AD) and studies of plaque cortical distribution, density, and morphology may lead to new information about the origin and pathogenesis of this disease. We have developed an automated computer image analysis program to detec...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hibbard LS,Arnicar-Sulze TL,McKeel DW Jr,Burrell LD

    更新日期:1994-06-01 00:00:00

  • A microprocessor-based tissue displacement monitor for in vitro quantification of knee capsule deformation.

    abstract::The advantages of utilizing a microprocessor design kit as a central element in custom laboratory instrumentation are demonstrated in the design of a real-time, multiple point, tissue displacement monitor. The tissue sample to be monitored is submerged in a saline bath, through which perpendicular electric fields are ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Looft FJ,Lyons HD

    更新日期:1984-02-01 00:00:00

  • Accurate quantitative RT-PCR for relative expression of Slo splice variants.

    abstract::Much interest has been shown in the use of multi-template reverse transcription-polymerase chain reaction (RT-PCR) as a quantitative instrument for low-abundance mRNAs. A desire to achieve finely-graded quantification of the stress- and hormone-related regulation of one splicing decision in an ion channel gene motivat...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mahmoud SF,Bezzerides AL,Riba R,Lai GJ,Lovell PV,Hara Y,McCobb DP

    更新日期:2002-04-15 00:00:00

  • Analysis of subcellular [57Co] cobalamin distribution in SH-SY5Y neurons and brain tissue.

    abstract::Cobalamin (Cbl) utilization as a cofactor for methionine synthase and methylmalonyl-CoA mutase is dependent on the transport of Cbl through lysosomes and its subsequent delivery to the cytosol and mitochondria. We speculated that neuropathological conditions that impair lysosomal function (e.g., age-related lipofuscin...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Zhao H,Ruberu K,Li H,Garner B

    更新日期:2013-07-15 00:00:00

  • Recording of spindle afferent discharge during hindlimb movements in spinalized turtles.

    abstract::The feasibility of spindle afferent recording during naturally or electrically induced hindlimb movements is demonstrated in the case of spinalized, decapitated turtles. Since these recordings are obtained from fine filaments of exposed dorsal roots, it is possible for the experimenter to select the afferents of inter...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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    更新日期:1986-02-01 00:00:00

  • Head movements and actographic recordings in free-moving animals, using computer analysis of video images.

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    journal_title:Journal of neuroscience methods

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  • Development of BMP7-producing human cells, using a third generation lentiviral gene delivery system.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Time-frequency component analyser and its application to brain oscillatory activity.

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  • Transfection of rat brain cells by electroporation.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • A simple intrapipette salt bridge.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kleene SJ

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  • A discovery of new features of gastropod local field potentials by application of wavelet tools.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Specificity and efficacy of noradrenaline, serotonin depletion in discrete brain areas of Swiss mice by neurotoxins.

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    pub_type: 杂志文章


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  • Developing a 3-choice serial reaction time task for examining neural and cognitive function in an equine model.

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    pub_type: 杂志文章


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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Functional assessments through novel proteomics approaches: Application to insulin/IGF signaling in neurodegenerative disease'.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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    更新日期:2019-05-01 00:00:00

  • Primary cell culture of adult zebrafish spinal neurons for electrophysiological studies.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Meade ME,Roginsky JE,Schulz JR

    更新日期:2019-07-01 00:00:00

  • Improving performance of motorized slides for micromanipulation.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Aspects of the quantitative analysis of neurons in the cerebral cortex.

    abstract::We address three problems concerning the quantitative analysis of nerve cell distribution in the cerebral cortex: (i) preparatory tissue deformation (shrinkage); (ii) difficulties in differentiating between small neurons and astroglia; and (iii) the bias introduced by the counting method. We found that staining with R...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Feasibility of electrical recordings from unconnected vertebrate CNS neurons cultured in a three-dimensional extracellular matrix.

    abstract::Single, i.e. unconnected spinal or cerebral neurons from chick embryos rapidly regenerate morphologically identifiable axons and dendrites when cultured in a three-dimensional (3-D) extracellular matrix (ECM) consisting of a hydrated native collagen lattice. We now show that it is possible to study the intrinsic elect...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Coates PW,Nathan RD

    更新日期:1987-07-01 00:00:00

  • Recording eye movements with video-oculography and scleral search coils: a direct comparison of two methods.

    abstract::A video-based 2D eye-tracking system (EyeLink version 2.04, SR Research Ltd/SMI) was compared with the scleral search coil technique for its performance on recording the properties of fixations and saccadic eye movements. Fixation positions and saccadic properties (amplitude, duration, and peak velocity) were calculat...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: van der Geest JN,Frens MA

    更新日期:2002-03-15 00:00:00

  • A nonparametric method for detecting fixations and saccades using cluster analysis: removing the need for arbitrary thresholds.

    abstract:BACKGROUND:Eye tracking is an important component of many human and non-human primate behavioral experiments. As behavioral paradigms have become more complex, including unconstrained viewing of natural images, eye movements measured in these paradigms have become more variable and complex as well. Accordingly, the com...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: König SD,Buffalo EA

    更新日期:2014-04-30 00:00:00

  • Anterograde tracing method using DiI to label vagal innervation of the embryonic and early postnatal mouse gastrointestinal tract.

    abstract::The mouse is an extremely valuable model for studying vagal development in relation to strain differences, genetic variation, gene manipulations or pharmacological manipulations. Therefore, a method using 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was developed for labeling vagal innervat...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Murphy MC,Fox EA

    更新日期:2007-07-30 00:00:00

  • Constructing fMRI connectivity networks: a whole brain functional parcellation method for node definition.

    abstract:BACKGROUND:Functional Magnetic Resonance Imaging (fMRI) is used for exploring brain functionality, and recently it was applied for mapping the brain connection patterns. To give a meaningful neurobiological interpretation to the connectivity network, it is fundamental to properly define the network framework. In partic...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Maggioni E,Tana MG,Arrigoni F,Zucca C,Bianchi AM

    更新日期:2014-05-15 00:00:00

  • Targeted knock-down of neuronal nitric oxide synthase expression in basal forebrain with RNA interference.

    abstract::Nitric oxide (NO) is a gas messenger with diverse physiological roles in the nervous system, from modulation of synaptic plasticity and neurogenesis to the mediation of neuronal death. NO production in the brain is catalyzed by three isoforms of NO synthase (NOS) including neuronal NOS (nNOS), inducible NOS and endoth...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mahairaki V,Xu L,Farah MH,Hatfield G,Kizana E,Marbán E,Koliatsos VE

    更新日期:2009-05-15 00:00:00

  • Stereotaxic 18F-FDG PET and MRI templates with three-dimensional digital atlas for statistical parametric mapping analysis of tree shrew brain.

    abstract:BACKGROUND:Tree shrews are proposed as an alternative animal model to nonhuman primates due to their close affinity to primates. Neuroimaging techniques are widely used to study brain functions and structures of humans and animals. However, tree shrews are rarely applied in neuroimaging field partly due to the lack of ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Huang Q,Nie B,Ma C,Wang J,Zhang T,Duan S,Wu S,Liang S,Li P,Liu H,Sun H,Zhou J,Xu L,Shan B

    更新日期:2018-01-01 00:00:00

  • A simple and inexpensive device for applying a calibration pulse when performing intracellular recording.

    abstract::A simple and low-cost device is described which facilitates the calibration of intracellular DC recordings in amplitude and time. A current source, optically coupled to a conventional stimulator, injects a pulse of current, across a 1-omega resistor connecting the preparation to ground. Amplitude and duration of the c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Godfraind JM,Veraart C,Nicaise M

    更新日期:1984-05-01 00:00:00

  • Death of photoreceptors in organotypic retinal explant cultures: implication of rhodopsin accumulation and endoplasmic reticulum stress.

    abstract::Here we suggest that endoplasmic reticulum (ER)-stress may be induced following aberrant rhodopsin accumulation in photoreceptors in explanted rat retinas. Rhodopsin accumulation was accompanied by increased phosphorylation of pancreatic ER-kinase and eukaryotic initiator factor 2α as well as increased levels of C/EBP...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mohlin C,Johansson K

    更新日期:2011-04-15 00:00:00

  • A microfluidic chip for axonal isolation and electrophysiological measurements.

    abstract::A microfluidic chip for culturing neurons and spatially isolating axons from somas is presented for use with visually guided whole-cell electrophysiological measurements. A modular design consisting of detachable and re-sealable layers is used to satisfy the requirements of both long-term neuron culturing as well as e...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Jokinen V,Sakha P,Suvanto P,Rivera C,Franssila S,Lauri SE,Huttunen HJ

    更新日期:2013-01-30 00:00:00

  • A method for the isolation of purified murine neuroepithelial cells from the developing mouse brain.

    abstract::The adult mammalian central nervous system develops from the pseudostratified neuroepithelium of the neural tube. In order to study, in vitro, the differentiation of the neuroepithelial cells in detail and to identify factors that may influence this process, an uncontaminated, viable population of neuroepithelial cell...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Drago J,Murphy M,Bailey KA,Bartlett PF

    更新日期:1991-05-01 00:00:00