Direct transdifferentiation of human Wharton's jelly mesenchymal stromal cells into cholinergic-like neurons.


:Barckground Alzheimer's disease (AD) is mainly caused by cellular loss and dysfunction of the basal forebrain cholinergic neurons and cholinergic axons in the cortex leading to slowly progressive decline in learning and memory performance. Unfortunately, no definitive treatment to halt neural cell loss exists to date. Therefore, it is necessary to obtain an unlimited source of cholinergic neurons for future pharmacological applications in AD. Human mesenchymal stromal cells (hMSCs) represent a unique source of cholinergic-like neurons (ChLNs). New method hWJ-MSCs were incubated with Cholinergic-N-Run medium for 4 and 7 days. Results hWJ-MSCs cultured with Cholinergic-N-Run medium differentiated into ChLNs in 4 days as evidenced by high levels of protein expression of the neuronal markers ChAT, VAChT, AChE, MAP2, β-Tubulin III, NeuN, TUC-4, NF-L and no expression of the immature marker SOX2, the dopaminergic marker TH, GABAergic marker GAD67 and glial marker GFAP. Comparison with existing method(s) The hWJ-MSCs form ChLNs (e.g., ∼26% IF+) within 20 days by using complex conditioned mediums that are expensive and time-consuming. We report for the first time, to our best knowledge, a direct method of hWJ-MSCs transdifferentiation into ChLNs (∼76% ChAT /VAChT assessed by immunofluorescence microscopy and flow cytometry) in an economic, efficient and timely fashion. Conclusions The fastest method to obtain ChLNs from hWJ-MSCs takes only four days using the one-step incubation medium Cholinergic-N-Run.


J Neurosci Methods


Mendivil-Perez M,Velez-Pardo C,Jimenez-Del-Rio M




Has Abstract


2019-01-15 00:00:00












  • A diffusion-reaction model of nerve regeneration.

    abstract::The process of peripheral nerve regeneration has been modeled using 5 populations of mathematical variables to represent the biological activities of Wallerian degeneration, fibrin matrix development, Schwann cell activity, elongating neurites, and neovascularization. The mathematical model provided simulations of ner...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Podhajsky RJ,Myers RR

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  • The impact of recent stereological advances on quantitative studies of the nervous system.

    abstract::The usefulness of a number of new stereological principles for unbiased estimation of particle number and sizes and sampling of particles is illustrated together with a novel principle for unbiased estimation of anisotropic surfaces. The examples include descriptions of estimators of neurone and synapse number and siz...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Overexpression of growth-associated proteins in the neurons of adult transgenic mice.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


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  • Development of new optical imaging systems of oxygen metabolism and simultaneous measurement in hemodynamic changes using awake mice.

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    journal_title:Journal of neuroscience methods

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  • Comparison of the efficacy of four viral vectors for transducing hypothalamic magnocellular neurosecretory neurons in the rat supraoptic nucleus.

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  • Two variables that can be used as pain indices in experimental animal models of arthritis.

    abstract::Since pain is an important symptom in arthritis, useful behavioral indices for pain in experimental arthritis animal models are important tools for investigative work on arthritis. The purpose of this study was to develop simple and quantifiable behavioral tests, which would represent the level of arthritic pain that ...

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  • Social behavior of zebrafish: from synthetic images to biological mechanisms of shoaling.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


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  • Quantitative analysis of acetylcholine in rat brain microdialysates by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.

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    journal_title:Journal of neuroscience methods

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    pub_type: 杂志文章


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  • Comparison of visually evoked local field potentials in isolated turtle brain: patterned versus blank stimulation.

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  • Visualizing and quantifying evoked cortical activity assessed with intrinsic signal imaging.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Quantitative evaluation of orofacial motor function in mice: The pasta gnawing test, a voluntary and stress-free behavior test.

    abstract:BACKGROUND:Evaluation of motor deficits in rodents is mostly restricted to limb motor tests that are often high stressors for the animals. NEW METHOD:To test rodents for orofacial motor impairments in a stress-free environment, we established the pasta gnawing test by measuring the biting noise of mice that eat a piec...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Neurochemical identification of fos-positive neurons using two-colour immunoperoxidase staining.

    abstract::The discovery of immediate early genes (IEG) has provided neuroscientists with a new functional mapping technique. Labelling of neural tissue for the protein product of IEG provides an activity map with single-cell resolution. When combined with labelling for the chemical identity of the neuron, this provides a powerf...

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  • Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays.

    abstract::In order to identify novel genes involved in appetite and body weight regulation we have developed a microarray based method suitable for detecting small changes in gene expression in discrete groups of hypothalamic neurons. The method is based on a combination of stereological sampling, laser capture microdissection ...

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  • Imprinting as a rapid technique for assessing the morphology of the central nervous system by immunofluorescence.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


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  • The use of whole-mount preparations of nerves labelled with axonally transported radioactive proteins to study regeneration.

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    journal_title:Journal of neuroscience methods

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  • Measurement of axonal conduction velocity in single mammalian motor axons.

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    pub_type: 杂志文章


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