An inexpensive and interactive microcomputer system for codifying Golgi-impregnated neuronal morphology.

Abstract:

:An interactive microcomputer system has been developed for the quantitative analysis of Golgi-impregnated neuronal morphology (GINM). The system uses commercially available hardware: a 48K RAM microcomputer (Apple II), a TV monitor (NEC), an optical microscope (Reichert Biovar) with a camera lucida and a specially developed wheel attached to the focus adjustment knob and to a digital planimeter (Tamaya), allowing one to obtain the value of the z-coordinate. The x- and y-coordinates are obtained using the movement of a point plotted by the computer on the TV monitor. This point is superimposed upon the GINM point to be codified employing the camera lucida. The x- and y-coordinates are acquired automatically while the variations of the z-coordinate are introduced by the computer keyboard. The three-dimensional coordinates of the selected GINM points and different codes are stored by the computer in a six-dimensional array. The software is written in BASIC. The results demonstrate that the digitization and quantitative analysis of GINM can be achieved with reasonably inexpensive equipment.

journal_name

J Neurosci Methods

authors

Freire M

doi

10.1016/0165-0270(86)90043-9

subject

Has Abstract

pub_date

1986-04-01 00:00:00

pages

103-17

issue

2

eissn

0165-0270

issn

1872-678X

pii

0165-0270(86)90043-9

journal_volume

16

pub_type

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