Determination of amino acids associated with cerebral ischaemia in rat brain microdialysates using narrowbore liquid chromatography and fluorescence detection.

abstract：:Since the advent of optogenetics, the technology development has focused on new methods to optically interact with single nerve cells. This gave rise to the field of photonic neural interfaces, intended as the set of technologies that can modify light radiation in either a linear or non-linear fashion to control and/o...

journal_title：Journal of neuroscience methods

authors： Pisano F,Pisanello M,De Vittorio M,Pisanello F

更新日期：2019-09-01 00:00:00

abstract：:Mutations in the human prion protein gene (PRNP) are responsible for hereditary diseases called transmissible spongiform encephalopathies (TSE) and a polymorphic site at codon 129 determines sensitivity to infectious forms of these maladies. More recently, codon 129 has been related to cognition performance in the eld...

journal_title：Journal of neuroscience methods

authors： Castro RM,Landemberger MC,Walz R,Carlotti CG Jr,Huang N,Cunha DR,Moura R,Caballero OL,Sakamoto AC,Nitrini R,Brentani RR,Martins VR

更新日期：2004-10-30 00:00:00

abstract：:The image-combining computer microscope is a new configuration of light microscope and computer graphics instrumentation which provides remarkable morphometric capabilities over a wide range of applications. The key component is the well known camera lucida, an image-combining device that is used to superimpose the co...

journal_title：Journal of neuroscience methods

authors： Glaser EM,Tagamets M,McMullen NT,Van der Loos H

更新日期：1983-05-01 00:00:00

abstract：:Both extracellular and intracellular stimulation of single motoneurons were shown to be similarly effective and consistent in eliciting contractile responses in single lateral rectus muscle motor units. The whole muscle was activated by stimulating the sixth nerve in the brain stem. Both whole muscle and motor unit co...

journal_title：Journal of neuroscience methods

authors： Goldberg SJ,Shall MS

更新日期：1997-12-30 00:00:00

abstract：:Technical challenges to record cortical unit spikes in conscious behavior monkeys have been presenting constraints in the investigation of cortical control of lower limb. Here we report a novel experimental system for investigation of correlation between cortical neuronal signals and standing and squatting movement. T...

journal_title：Journal of neuroscience methods

authors： Ma C,He J

更新日期：2010-09-30 00:00:00

abstract：:Presented in this short communication are design principles for the construction of a positioning apparatus for biological specimens in magnetically sensitive environments. An apparatus with 3 degrees of freedom was built and found to provide position accuracy to within +/- 0.25 mm throughout a cubic volume measuring ...

journal_title：Journal of neuroscience methods

authors： MacHattie DB,Pengelly LD

更新日期：1992-08-01 00:00:00

abstract：BACKGROUND:Sympathetic nerve wiring in the mammalian heart has remained largely unexplored. Resolving the wiring diagram of the cardiac sympathetic network would help establish the structural underpinnings of neurocardiac coupling. NEW METHOD:We used two-photon excitation fluorescence microscopy, combined with a compu...

journal_title：Journal of neuroscience methods

authors： Freeman K,Tao W,Sun H,Soonpaa MH,Rubart M

更新日期：2014-01-15 00:00:00

abstract：:Parkinson's disease (PD) exhibits symptoms of motor dysfunction such as tremor, akinesia and rigidity. Agents that selectively disrupt or destroy catecholaminergic systems, such as reserpine, methamphetamine, 6-hydroxydopamine and 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine, have been used to develop PD models and to...

journal_title：Journal of neuroscience methods

authors： Luthra PM,Barodia SK,Raghubir R

更新日期：2009-04-15 00:00:00

abstract：:Genetic intervention is a powerful means for answering basic biological questions about molecular events taking place in brain cells underlying complex behaviors. Traditional transfection method has proved difficult to apply to neuronal cells. We therefore described an alternative procedure for introducing DNA into cu...

journal_title：Journal of neuroscience methods

authors： Li H,Chan ST,Tang F

更新日期：1997-07-18 00:00:00

abstract：:We describe an immunocytochemical method for marking microelectrode tracks made during single-unit recordings in long surviving, awake monkeys. This procedure detects the increase in glial fibrillary acidic protein in the glial cells along a microelectrode track using commercially available antibodies. We have success...

journal_title：Journal of neuroscience methods

authors： Benevento LA,McCleary LB

更新日期：1992-03-01 00:00:00

abstract：BACKGROUND:Intracranial electrical recordings (iEEG) and brain stimulation (iEBS) are invaluable human neuroscience methodologies. However, the value of such data is often unrealized as many laboratories lack tools for localizing electrodes relative to anatomy. To remedy this, we have developed a MATLAB toolbox for int...

journal_title：Journal of neuroscience methods

authors： Groppe DM,Bickel S,Dykstra AR,Wang X,Mégevand P,Mercier MR,Lado FA,Mehta AD,Honey CJ

更新日期：2017-04-01 00:00:00

abstract：:In deeply anesthetized cats, determinations of motor-axonal conduction velocity (CV) were made using extracellular potentials recorded from single, functionally isolated motor axons innervating the muscle tibialis posterior. Axons were activated by suprathreshold electrical stimulation at the ventral-root level. Actio...

journal_title：Journal of neuroscience methods

authors： Gordon DA,Hamm TM,Enoka RM,Reinking RM,Windhorst U,Stuart DG

更新日期：1987-04-01 00:00:00

abstract：:The use of a devitalized skeletal muscle graft and conventional nerve graft to repair a 5mm long segmentary sciatic nerve lesion was studied in rats by means of functional, morphometric and spinal cord motor neuron cell response evaluation. Thirty-four rats were used and divided into four groups according to the proce...

journal_title：Journal of neuroscience methods

authors： Oliveira EF,Mazzer N,Barbieri CH,DelBel EA

更新日期：2004-02-15 00:00:00

abstract：:Morphometric estimation of neuronal processes is currently laborious and time-consuming, since the individual processes (axons and dendrites) have to be traced manually. In order to facilitate the measurement of cellular processes, we have tested a series of parameters derived from the contour and the convex hull of a...

journal_title：Journal of neuroscience methods

authors： Kawa A,Stahlhut M,Berezin A,Bock E,Berezin V

更新日期：1998-01-31 00:00:00

abstract：:A method is described for the isolation of neurons from defined regions of the mammalian central nervous system, by a combination of mechanical and enzymatic means. The procedure liberates neurons free of cellular debris and glial investments, allowing the formation of giga-ohm seals with patch clamp electrodes. The c...

journal_title：Journal of neuroscience methods

authors： Kay AR,Wong RK

更新日期：1986-05-01 00:00:00

abstract：:A new simple technique for the accurate modification of microelectrode and micropipette tips by breaking has been developed. The breaking is done by longitudinally inserting a micropipette into a larger previously broken pipette for stability and by applying a force on the micropipette to cause it to break. Tips will ...

journal_title：Journal of neuroscience methods

authors： Briano RA Jr

更新日期：1983-09-01 00:00:00

abstract：:We have characterized a method of labeling of axons in the post-mortem spinal cord using a silastic disc holding pins coated with DiI and DiO at the rostral and caudal ends of the cord. We optimized the DiI and DiO tracing techniques under different conditions of fixative concentration (1% versus 4% paraformaldehyde, ...

journal_title：Journal of neuroscience methods

authors： Chen BK,Miller SM,Mantilla CB,Gross L,Yaszemski MJ,Windebank AJ

更新日期：2006-06-30 00:00:00

abstract：BACKGROUND:Negative emotional states resulting from concussion are of increasing concern. In the current study, we developed a model to investigate negative affect following concussion in the projectile concussive impact (PCI) model. High frequency ultrasonic vocalizations (22kHz USVs) are associated with negative affe...

journal_title：Journal of neuroscience methods

authors： Browning JR,Whiteman AC,Leung LY,Lu XM,Shear DA

更新日期：2017-01-01 00:00:00

abstract：:The "pipette" or "growth cone turning" assay is widely used for studying how axons respond to diffusible guidance cues in their environment. However, little quantitative analysis has been presented of the gradient shapes produced by this assay, or how they depend on parameters of the assay. Here we used confocal micro...

journal_title：Journal of neuroscience methods

authors： Pujic Z,Giacomantonio CE,Unni D,Rosoff WJ,Goodhill GJ

更新日期：2008-05-30 00:00:00

abstract：:Songbirds are the preeminent animal model for understanding how the brain encodes and produces learned vocalizations. Here, we report a new statistical method, the Kullback-Leibler (K-L) distance, for analyzing vocal change over time. First, we use a computerized recording system to capture all song syllables produced...

journal_title：Journal of neuroscience methods

authors： Wu W,Thompson JA,Bertram R,Johnson F

更新日期：2008-09-15 00:00:00

abstract：:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...

journal_title：Journal of neuroscience methods

authors： Justice JB Jr,Nicolaysen LC,Michael AC

更新日期：1988-01-01 00:00:00

abstract：BACKGROUND:Extraneural cuffs are among the least invasive peripheral nerve interfaces as they remain outside the nerve. However, compared with more invasive interfaces, these electrodes may suffer from lower selectivity and sensitivity since the targeted nerve fibers are more distanced from the electrodes. NEW METHOD:...

journal_title：Journal of neuroscience methods

authors： Elyahoodayan S,Larson C,Cobo AM,Meng E,Song D

更新日期：2020-04-15 00:00:00

abstract：:This paper presents an atlas of the rat brain based on horizontal sections stained for acetylcholinesterase (AChE). Fourteen labeled photographs from sections at 0.5 mm intervals are presented. The atlas is intended for use with male rats 250-300 g in weight. It was constructed using the flat-skull position (lambda an...

journal_title：Journal of neuroscience methods

authors： Paxinos G,Watson CR,Emson PC

更新日期：1980-12-01 00:00:00

abstract：:Following bilateral injection of manganese (Mn) into the rat's motor cortex, electrical stimulation of the cortex is shown to increase the transport, uptake and accumulation of Mn in the corticospinal tract (CST), as assessed by manganese-enhanced magnetic resonance imaging (MEI). T(1)-weighted gradient echo images we...

journal_title：Journal of neuroscience methods

authors： Bilgen M,Peng W,Al-Hafez B,Dancause N,He YY,Cheney PD

更新日期：2006-09-30 00:00:00

abstract：:Although dopamine (DA) translocation by the DA transporter (DAT) requires Na+, a role for Na+ in the DA recognition step in the translocation cycle has been questioned. Thus, when binding techniques were used to indirectly measure the affinity of DA for DAT via its potency in inhibiting cocaine analog binding, no stim...

journal_title：Journal of neuroscience methods

authors： Wang LC,Cui XN,Chen N,Reith ME

更新日期：2003-12-30 00:00:00

abstract：BACKGROUND:Tree shrews are proposed as an alternative animal model to nonhuman primates due to their close affinity to primates. Neuroimaging techniques are widely used to study brain functions and structures of humans and animals. However, tree shrews are rarely applied in neuroimaging field partly due to the lack of ...

journal_title：Journal of neuroscience methods

authors： Huang Q,Nie B,Ma C,Wang J,Zhang T,Duan S,Wu S,Liang S,Li P,Liu H,Sun H,Zhou J,Xu L,Shan B

更新日期：2018-01-01 00:00:00

abstract：BACKGROUND:Contralateral seventh cervical nerve transfer (contralateral C7 transfer) is a novel treatment for patients with spastic paralysis, including stroke and traumatic brain injury. However, little is known on changes in plasticity that occur in the intact hemisphere after C7 transfer. An appropriate surgical mod...

journal_title：Journal of neuroscience methods

authors： Ye X,Shen YD,Feng JT,Wang F,Gao ZR,Lei GW,Yu AP,Wang CP,Liang CM,Xu WD

更新日期：2019-12-01 00:00:00

abstract：:The scanning electron microscope (SEM) is a useful tool in the study of the retina of lower vertebrates. SEM analysis (preparation over the critical point) offers a useful addition to the existing histological preparations. It is suitable for a comparative analysis, in which the arrangement of different cellular eleme...

journal_title：Journal of neuroscience methods

authors： Breidbach O

更新日期：1984-11-01 00:00:00

abstract：:An important characterization of neural spiking is the ratio of the variance to the mean of the spike counts in a set of intervals--the Fano factor. For a Poisson process, the theoretical Fano factor is exactly one. For simulated or experimental neural data, the sample Fano factor is never exactly one, but often appea...

journal_title：Journal of neuroscience methods

authors： Eden UT,Kramer MA

更新日期：2010-06-30 00:00:00

abstract：:The effect of acute ethanol on adenosine content in four motor areas of the male Sprague-Dawley rat brain was investigated using HPLC-fluorescence detection. Since basal adenosine levels are difficult to assess due to extremely rapid turnover of adenosine, four different methods of sacrifice were also evaluated for ad...

journal_title：Journal of neuroscience methods

authors： Clark M,Dar MS

更新日期：1988-10-01 00:00:00