Abstract：
:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear differential equations. Parameters of the model are optimized with respect to diverse experimental data. These data include steady state passage of radioactivity, decline in total dopamine after synthesis inhibition, and change in extracellular dopamine concentration during electrical stimulation.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Justice JB Jr,Nicolaysen LC,Michael ACdoi
10.1016/0165-0270(88)90045-3subject
Has Abstractpub_date
1988-01-01 00:00:00pages
239-52issue
3eissn
0165-0270issn
1872-678Xpii
0165-0270(88)90045-3journal_volume
22pub_type
杂志文章abstract：:The immunotoxin 192 IgG-saporin has a high degree of selectivity for cholinergic neurons within the basal forebrain (CBF). Intracerebroventricular delivery of 192 IgG-saporin results in a diffuse and massive depletion of choline acetyltransferase (ChAT) activity in projections of the CBF, and non-selective loss of Pur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00057-6
更新日期：1999-09-15 00:00:00
abstract：:The quantification of synapses in neuronal cultures is essential in studies of the molecular mechanisms underlying synaptogenesis and synaptic plasticity. Conventional counting of synapses based on morphological or immunocytochemical criteria is extremely work-intensive. We developed a fully automated method which qua...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.11.010
更新日期：2012-02-15 00:00:00
abstract：BACKGROUND:Image segmentation is often imperfect, particularly in complex image sets such z-stack micrographs of slice cultures and there is a need for sufficient details of parameters used in quantitative image analysis to allow independent repeatability and appraisal. NEW METHOD:For the first time, we have criticall...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.12.002
更新日期：2018-02-01 00:00:00
abstract：:Synaptic connections between retinal cells were studied by recording simultaneously from pre- and postsynaptic cells in the retinal slice preparation. The time course and waveform of the pre- and postsynaptic light responses were monitored, and the postsynaptic voltage responses to presynaptic current injection were m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90046-x
更新日期：1987-06-01 00:00:00
abstract：:Characterizing the structure and electrophysiological properties of single neurons is essential for understanding how individual cells contribute to the function of neuronal networks. Following intra-cellular recording from neurons in acute brain slices, the structure of the recorded cell has typically been examined b...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00341-2
更新日期：2003-02-15 00:00:00
abstract：:Exponential decay of the power spectrum is typically observed in turbulent flow patterns, as evidenced both in experiments and in the Navier-Stokes equations describing fluid dynamics. In this study, we present evidence on the exponential decay of power spectra belonging to interspike interval time series, registered ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.01.022
更新日期：2011-04-15 00:00:00
abstract：BACKGROUND:Previous studies have measured whisker movements and locomotion to characterise mouse models of neurodegenerative disease. However, these studies have always been completed in isolation, and do not involve standardized procedures for comparisons across multiple mouse models and background strains. NEW METHO...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2019.108532
更新日期：2020-02-01 00:00:00
abstract：:Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.09.034
更新日期：2009-02-15 00:00:00
abstract：:Electrophysiological recording provides important evidence for positive identification of many neurons in gastropods. We describe a technique which combines intracellular recording and injection of a persistent, non-fluorescent dye (Fast Green) with subsequent histofluorescence treatment using a modification of the wh...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90036-6
更新日期：1985-08-01 00:00:00
abstract：:This research evaluated changes in surface electromyographic (SEMG) spike shape across different levels of isometric force. Ninety-six subjects generated three 5-s isometric step contractions of the elbow flexors at 40, 60, 80, and 100% of maximal voluntary contraction (MVC). Force and bipolar SEMG activity were monit...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.07.004
更新日期：2007-01-15 00:00:00
abstract：:We present a new analytical method for characterizing the directional tuning of neural data. The method is based on computing parameters associated with the geometric properties of solids, and provides an estimate of preferred direction in the context of non-uniform sampling of directions. Unlike optimization methods ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00492-7
更新日期：2002-01-30 00:00:00
abstract：:We assessed the potential of using multielectrode arrays (MEAs) to investigate several physiological properties of the calyx of Held synapse in the medial nucleus of the trapezoid body of gerbil. Due to the large size of the synapse, it became widely employed in studies on synaptic mechanisms. Electrical stimulation a...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.07.015
更新日期：2008-09-30 00:00:00
abstract：:The rates of deterioration of immunocytochemical staining for 4 peptide and 2 neuronal antigens were studied on human, guinea pig and rat colon and urinary bladder. It was found that if the fixation started within 10 h after surgery or death, little change was seen in the pattern and intensity of the immunostains. Whe...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90014-7
更新日期：1985-02-01 00:00:00
abstract：:Two-photon laser scan microscopy carries many advantages for work on brain slices and bulk tissue. However, it has very low signal levels compared to conventional fluorescence microscopy. This is disadvantageous in fast imaging applications when photon shot noise is limiting. Working on brain slices with excitation po...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00103-x
更新日期：1999-10-15 00:00:00
abstract：:Proteomic studies offer enormous potential for gaining insight into cellular dynamics and disease processes. An immediate challenge for enhancing the utility of proteomics in translational research lies in methods of handling and interpreting the large datasets generated. Publications rarely extend beyond lists of pro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.03.013
更新日期：2009-06-15 00:00:00
abstract：:One of the hallmark pathologies associated with Alzheimer's disease (AD) is the conspicuous deposition of extracellular amyloid plaques within the forebrain. These plaques are primarily composed of fibrular aggregates of the A-beta peptide. Traditional methods for the histological localization of these plaques typical...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.05.019
更新日期：2012-07-30 00:00:00
abstract：:Since no simple method for standardized positioning of very young rats in a stereotaxic device has been described in the literature, a new method for stereotaxic placement in the neonatal rat was developed, which only requires a simple body mould of the rat pup, and a minor adaptation of a rabbit stereotaxic apparatus...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90122-0
更新日期：1985-07-01 00:00:00
abstract：:Experimental recordings from neurons are generally difficult to quantify. Spike patterns and membrane potential cycles are often compared qualitatively and are hence limited by human resolution and judgement. This paper describes a statistical method for quantifying individual spike patterns and membrane potential flu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90053-t
更新日期：1993-10-01 00:00:00
abstract：:Operant animal behavioral tests require the interaction of the subject with sensors and actuators distributed in the experimental environment of the arena. In order to provide user independent reliable results and versatile control of these devices it is vital to use an automated control system. Commercial systems for...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.06.020
更新日期：2007-10-15 00:00:00
abstract：:Since the advent of optogenetics, the technology development has focused on new methods to optically interact with single nerve cells. This gave rise to the field of photonic neural interfaces, intended as the set of technologies that can modify light radiation in either a linear or non-linear fashion to control and/o...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2019.108355
更新日期：2019-09-01 00:00:00
abstract：:Senile plaques (SP) are the most characteristic neuropathologic lesions of Alzheimer's disease (AD) and studies of plaque cortical distribution, density, and morphology may lead to new information about the origin and pathogenesis of this disease. We have developed an automated computer image analysis program to detec...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)90128-7
更新日期：1994-06-01 00:00:00
abstract：:The "pipette" or "growth cone turning" assay is widely used for studying how axons respond to diffusible guidance cues in their environment. However, little quantitative analysis has been presented of the gradient shapes produced by this assay, or how they depend on parameters of the assay. Here we used confocal micro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.014
更新日期：2008-05-30 00:00:00
abstract：:Classification of spoken word-evoked potentials is useful for both neuroscientific and clinical applications including brain-computer interfaces (BCIs). By evaluating whether adopting a biology-based structure improves a classifier's accuracy, we can investigate the importance of such structure in human brain circuitr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.10.034
更新日期：2019-01-01 00:00:00
abstract：:The FD Rapid GolgiStain Kit (FD NeuroTechnologies, Inc., Ellicott City, MD) was developed using an improved Golgi-Cox impregnation method to provide stable, sensitive, and convenient staining of mature neurons. However, embryonic neurons cannot be clearly visualized using this method. We have further improved the Golg...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.06.007
更新日期：2012-07-30 00:00:00
abstract：:Neuronal activity causes substantial Na+ transients in fine cellular processes such as dendrites and spines. The physiological consequences of such Na+ transients are still largely unknown. High-resolution Na+ imaging is pivotal to study these questions, and, up to now, two-photon imaging with the fluorescent Na+ indi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.01.009
更新日期：2006-09-15 00:00:00
abstract：:The relationship between the levels of cytochrome oxidase and gamma-aminobutyric acid (GABA) was investigated within single neurons by double labeling the 2 markers in the same section. Double staining was equally effective when immunogold-silver staining of GABA was followed by indirect immunoperoxidase labeling of c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90129-5
更新日期：1989-12-01 00:00:00
abstract：:The aim of this work is to define neurotoxins doses to have efficient and specific depletion of noradrenaline (NA), serotonin (5-HT) neurotransmission in cortex, striatum, hippocampus and hypothalamus of Swiss mice after intraperitoneal administration of, respectively, N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.06.008
更新日期：2006-01-15 00:00:00
abstract：:Chronic spinal catheterization via an atlanto-occipital puncture (CAO) has been widely used to study the effects of drugs on spinal nociceptive mechanisms, but this method is associated with spinal cord damage that may change the efficacy of spinally injected analgesics. Using a slight modification of the method of St...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00197-3
更新日期：2003-10-15 00:00:00
abstract：:The permanence of retrograde neuronal labeling by the fluorescent tracers Fast Blue, Fluoro-Gold, Mini-Ruby, Fluoro-Ruby and Fluoro-Emerald was investigated in adult rat spinal motorneurons at 1, 4, 12 and 24 weeks after tracer application to a transected muscle nerve. After 1 week, the largest number of retrogradely ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)02227-9
更新日期：1997-06-06 00:00:00
abstract：BACKGROUND:The ongoing crisis related to non-medical use of opioids makes it of continued importance to understand the risk factors for opioid addiction, the behavioral and neurobiological consequences of opioid exposure and to seek potential avenues for therapy. Pre-clinical rodent models have been critical to advanci...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108993
更新日期：2021-01-15 00:00:00
