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Introducing Amylo-Glo, a novel fluorescent amyloid specific histochemical tracer especially suited for multiple labeling and large scale quantification studies.

Abstract：

:One of the hallmark pathologies associated with Alzheimer's disease (AD) is the conspicuous deposition of extracellular amyloid plaques within the forebrain. These plaques are primarily composed of fibrular aggregates of the A-beta peptide. Traditional methods for the histological localization of these plaques typically rely on the use of the tracers Congo Red or Thioflavin S. This study describes the characterization of a novel fluorescent histochemical probe, Amylo-Glo, for the high resolution and contrast localization of amyloid plaques in brain tissue sections. Potential advantages over conventional amyloid plaque stains such as Congo Red or Thioflavin S can be attributed to its unique chemical and spectral properties. Specifically, it results in a very bright blue UV excitable stain under physiological conditions that will not bleed through when illuminated with other filters. Its brightness makes it ideal for low magnification quantification studies, while its unique excitation/emission profile and mild staining conditions makes it ideal for combination with multiple immunofluorescent labeling studies.

journal_name

J Neurosci Methods

journal_title

Journal of neuroscience methods

authors

Schmued L,Raymick J,Tolleson W,Sarkar S,Zhang YH,Bell-Cohn A

doi

10.1016/j.jneumeth.2012.05.019

subject

Has Abstract

pub_date

2012-07-30 00:00:00

pages

120-6

issue

1

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(12)00188-4

journal_volume

209

pub_type

杂志文章

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