Abstract：
BACKGROUND:The trigeminal subnucleus caudalis (Vc) plays a critical role in transmission and modulation of nociceptive afferent inputs, and exhibits a similar layer construction to the spinal dorsal horn. However, afferent inputs enter the brainstem and project to a separately located nucleus. It has previously been difficult to record responses of the Vc to afferent fiber activation in a brainstem slice preparation. The aim of the present study was to establish a novel brainstem slice preparation method to study trigeminal nociceptive transmission mechanisms. NEW METHOD:Thirty adult 6-7-week-old C57/BL6J male mice were included in the study. Obliquely sliced brainstem sections at a thickness of 600μm, which included the Vc and the root entry zone to the brainstem, were prepared. The Vc response to electrical stimulation of afferent fibers was observed as a change in intracellular calcium concentration by fluorescence intensity response. RESULTS:Electrical stimulation of afferent inputs to the trigeminal nerve increased fluorescent intensity in the Vc, which was completely diminished by tetrodotoxin and significantly suppressed by the AMPA/kainate antagonist CNQX (paired t-test, P<0.001), although the non-competitive NMDA antagonist (+)-MK801 maleate resulted in no changes. These results suggested a glutamate receptor-mediated response. COMPARISON WITH EXISTING METHODS/CONCLUSION:This brainstem slice preparation will be useful for investigating nociceptive transmission mechanisms of the trigeminal nerve.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Hirahara M,Fujiwara N,Seo Kdoi
10.1016/j.jneumeth.2017.05.019subject
Has Abstractpub_date
2017-07-15 00:00:00pages
6-15eissn
0165-0270issn
1872-678Xpii
S0165-0270(17)30146-2journal_volume
286pub_type
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