Abstract:
:Imaging technologies, such as intrinsic optical imaging (IOI), functional magnetic resonance imaging (fMRI) or multiphoton microscopy provide excellent opportunities to study the relationship between functional signals recorded from a cortical area and the underlying anatomical structure. This, in turn, requires accurate alignment of the recorded functional imaging data with histological datasets from the imaged tissue obtained after the functional experiment. This alignment is complicated by distortions of the tissue which naturally occur during histological treatment, and is particularly difficult to achieve over large cortical areas, such as primate visual areas. We present here a method that uses IOI vessel maps revealed in the time course of the intrinsic signal, in combination with vascular casts and vascular lumen labeling techniques together with a pseudo three dimensional (p3D) reconstruction of the tissue architecture in order to facilitate alignment of IOI data with posthoc histological datasets. We demonstrate that by such a multimodal vessel mapping approach, we are able to constitute a hook in anatomical-functional data alignment that enables the accurate assignment of functional signals over large cortical regions. As an example, we present precise alignments of IOI responses showing orientation selectivity of primate V1 with anatomical sections stained for cytochrome-oxidase-reactivity.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Valverde Salzmann MF,Wallace DJ,Logothetis NK,Schüz Adoi
10.1016/j.jneumeth.2011.07.029subject
Has Abstractpub_date
2011-09-30 00:00:00pages
159-72issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(11)00458-4journal_volume
201pub_type
杂志文章abstract::A system for autoregulation of body temperature of large and small animals is described. The device uses the IC AD590 as a temperature transducer. It operates on the basis of continuous regulation of the heating current, and does not emit transients which interfere with electrical recordings. A panel meter shows eithe...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90128-3
更新日期:1989-12-01 00:00:00
abstract::The assumptions upon which a correct interpretation of the t-test depends are rarely fulfilled by data from the neurosciences. This applies to both independent and correlated samples. The Mann-Whitney U-test is suggested as an alternative for the t-test for independent samples. The way in which significant results fro...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/0165-0270(83)90058-4
更新日期:1983-12-01 00:00:00
abstract::A procedure is described by which the solid silver chromate precipitate present in Golgi-impregnated neurons is reduced by a photographic developer into metallic silver grains or particles. The remaining silver chromate is subsequently removed, and the fine structure and synaptic relationships of the impregnated neuro...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(83)90023-7
更新日期:1983-04-01 00:00:00
abstract:BACKGROUND:Over 90 years after its first recording, scalp electroencephalography (EEG) remains one of the most widely used techniques in human neuroscience research, in particular for the study of event-related potentials (ERPs). However, because of its low signal-to-noise ratio, extracting useful information from thes...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.12.014
更新日期:2018-02-15 00:00:00
abstract::We have developed and characterized a method for the rapid autoradiographic determination of receptor sites for the non-competitive NMDA receptor antagonist, MK-801, using an iodinated form of the compound, (+)-3-[125I]-iodo-MK-801. The binding site was shown to exhibit those criteria necessary for its definition as a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90137-g
更新日期:1993-01-01 00:00:00
abstract::The mitochondrial DNA (mtDNA) depletion status of rho(0) cell lines is typically assessed by hybridization or polymerase chain reaction (PCR) experiments, in which the failure to hybridize mtDNA or amplify mtDNA using mtDNA-directed primers suggests thorough mitochondrial genome removal. Here, we report the use of an ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.06.023
更新日期:2006-01-30 00:00:00
abstract::The use of c-fos protein (Fos) immunocytochemistry as a metabolic marker for tracing neuroanatomical connections, seizure pathways and sites of action of neuroactive drugs is discussed in this report. Fos immunocytochemistry will be very useful for these purposes providing that a number of potential problems are recog...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/0165-0270(89)90150-7
更新日期:1989-09-01 00:00:00
abstract::Synthesis of melatonin in the mammalian pineal gland is regulated by the rhythmic expression of acetyl-CoA: serotonin N-acetyltransferase (SNAT) and other unknown factors. To screen for pineal-specific mRNAs potentially involved in melatonin synthesis and/or regulation, differential display PCR (DDPCR) was employed. W...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)02237-1
更新日期:1997-05-16 00:00:00
abstract::Precisely localized lesions in cuticular structures of insects can be produced by fine glass rods, vibrating transversally at frequencies above 100 kHz. The oscillating edge of the glass rod scrapes off the chitinous material at minimal elastic deformation during treatment. The achieved roughness height is in the 0.1 ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90111-8
更新日期:1989-07-01 00:00:00
abstract::Transplantation of human fetal ventral mesencephalon (VM) to Parkinsonian patients has shown beneficial effects in several clinical trials. However, further improvements in the transplantation technique are needed. Delayed surgery, i.e., the in vitro maintenance of the tissue prior to transplantation would present sev...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(94)90160-0
更新日期:1994-09-01 00:00:00
abstract::A biotinylated lectin from Ricinus communis (RCA-I) and avidin-biotin-horseradish peroxidase complex (ABC) were used to identify microglial cells in 3-microns-thick sections of formalin-fixed paraffin-embedded brains of adult mice required for quantitative cell kinetic studies. In 3-microns-thick sections of the mouse...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90034-o
更新日期:1993-12-01 00:00:00
abstract:BACKGROUND:Dilated brain perivascular spaces (PVSs) are found to be associated with many conditions, including aging, dementia, and Alzheimer's disease (AD). Conventionally, PVS assessment is mainly based on subjective observations of the number, size and shape of PVSs in MR images collected at clinical field strengths...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.09.001
更新日期:2015-12-30 00:00:00
abstract::Fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) provides exquisite temporal and spatial resolution for monitoring brain chemistry. The utility of this approach has recently been demonstrated by measuring sub-second dopamine changes associated with behavior. However, one drawback is the cable...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.04.043
更新日期:2004-12-30 00:00:00
abstract::Microglia and astrocytes play vital roles in normal human brain function and in neurological disorders. To study their physiological and pathological roles it is desirable to establish in vitro systems that are derived from the adult human brain. Although several groups have successfully cultured cells from the human ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.07.005
更新日期:2007-10-15 00:00:00
abstract::Xenopus laevis oocytes were injected with synthetic mRNA coding for a rat VMAT2 mutant (rVMAT2-I483A/L484A) shown previously to be retained on the plasma membrane as a result of a presumed reduction of endocytosis. Binding of the specific VMAT inhibitor [3H]dihydrotetrabenazine indicated that expression did occur at a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2003.10.010
更新日期:2004-02-15 00:00:00
abstract::A protocol has been established for the staining of myelin in frozen sections. While the new method is relatively fast and simple, it eliminates the problems routinely encountered with myelin stains such as blotchiness and uneven staining. Modifications were introduced into the procedures in order to obtain excellent ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(83)90012-2
更新日期:1983-03-01 00:00:00
abstract::Cytochrome c oxidase or mitochondrial respiratory chain complex IV is where over 90% of oxygen is consumed. The relationship between complex IV activity and mitochondrial proteins, which provides a guide to understanding the mechanisms in primary mitochondrial disorders, has been determined by histochemistry (activity...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.08.020
更新日期:2009-11-15 00:00:00
abstract:BACKGROUND:Intracerebral hemorrhage (ICH) is a stroke subtype with the highest mortality rate. Hematoma expansion and re-bleeding post-ICH are common and exacerbate the initial cerebral insult. There is a need for continuous monitoring of the neurologic status of patients with an ICH injury. NEW METHOD:A prototype dev...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.05.025
更新日期:2014-09-30 00:00:00
abstract::Chimera construction between different proteins is a useful method for investigating protein structure and function relationships. However, this technique, by its traditional application, has been daunting because of its complex procedure and low success rate. Here we describe a protocol for constructing chimeras betw...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.08.033
更新日期:2010-10-30 00:00:00
abstract::Facial expressions of emotions are important in nonverbal communication. Although numerous neural structures have been identified to be involved in emotional face processing, the amygdala is thought to be a core moderator. While previous studies have relied on facial images of humans, the present study is concerned wi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.10.016
更新日期:2007-03-30 00:00:00
abstract:BACKGROUND:The local field potential (LFP) is usually calculated from current sources arising from transmembrane currents, in particular in asymmetric cellular morphologies such as pyramidal neurons. NEW METHOD:Here, we adopt a different point of view and relate the spiking of neurons to the LFP through efferent synap...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108871
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND:Demyelination is the end product of numerous pathological processes, and also is one of the main causes of neurological disability in Multiple sclerosis (MS). Research into the pathogenesis of MS is hampered by the conventional rodent models' inability to produce stable demyelination. NEW METHOD:Focal demye...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.05.024
更新日期:2018-09-01 00:00:00
abstract::We investigated whether conscious rats exhibit hyperalgesia to noxious mechanical stimuli during tail reperfusion following transient tail ischaemia. Mechanical stimuli were delivered by three mechanical algometers, differing in contact area, geometry and rate of application. Tail ischaemia was induced by applying a p...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00313-7
更新日期:2000-11-30 00:00:00
abstract::NREM phases of sleep in vertebrates are characterized by slow waves. Crayfish also sleeps while lying on one side on the surface of the water. At this time the numerous spikes on an almost flat base line generated by the brain when alert are replaced by slow waves of 15-20 Hz. In this work, we conducted experiments to...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.01.025
更新日期:2007-05-15 00:00:00
abstract::Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common genetic cause of Parkinson's disease. LRRK2 kinase activity is required for toxicity in neuronal cell cultures suggesting that selective kinase inhibitors may prevent neurodegeneration in patients. Directly monitoring LRRK2 activity in cells would b...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.12.015
更新日期:2013-03-30 00:00:00
abstract::The development and effective use of robust high-electrode-count microelectrode arrays for neuronal recording and stimulation depends on effective monitoring of electrode impedances and how these change over time. In multielectrode arrays, conventional electrode impedance measurements may be confounded by possible shu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.12.020
更新日期:2009-04-15 00:00:00
abstract::This paper describes a technique that has been developed to assess the in vivo morphology of central nervous system (CNS) tissue by immunofluorescence. This technique permits the study of tissue that is mainly just a monolayer of cells. Unlike routine cryosections that are much thicker (10-15 microm), imprinting does ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00237-5
更新日期:2000-07-31 00:00:00
abstract::Electrical stimulation of the rat incisor pulp has been frequently used as a method to study the mechanisms of pain. However, several of the techniques used to stimulate nociceptive nerves in these teeth also tend to excite non-nociceptive nerves in adjacent tissues. This report deals with the development and verifica...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(87)90092-6
更新日期:1987-11-01 00:00:00
abstract::Brain activity often consists of interactions between internal-or on-going-and external-or sensory-activity streams, resulting in complex, distributed patterns of neural activity. Investigation of such interactions could benefit from closed-loop experimental protocols in which one stream can be controlled depending on...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.02.020
更新日期:2013-04-30 00:00:00
abstract::Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.09.034
更新日期:2009-02-15 00:00:00