Abstract：
:Synthesis of melatonin in the mammalian pineal gland is regulated by the rhythmic expression of acetyl-CoA: serotonin N-acetyltransferase (SNAT) and other unknown factors. To screen for pineal-specific mRNAs potentially involved in melatonin synthesis and/or regulation, differential display PCR (DDPCR) was employed. We used 80 primer pairs and examined 40 bands of interest. One of the pineal specific clones (relative to brain and eye), PG25, was studied further. Hybridization histochemical and Northern analyses confirmed its tissue specificity. The size of the corresponding mRNA is 2.4 kb. A cDNA (2 kb) containing the coding region was obtained using a long-template PCR-based RACE technique. A data base search indicates that PG25 is highly homologous to a recently identified human lung endothelial cell-specific gene, ESM-1. Interestingly, not only the amino acid sequences but also the cDNA sequences, including the long 3' untranslated regions, are highly similar. This suggests that the conserved 3' untranslated region may carry information to regulate its own expression. Northern analysis revealed that PG25 is also expressed in the rat lung, but at a much lower (10%) level compared to the pineal. Finally, our work shows the feasibility of a fast, integrated PCR-based cloning method for obtaining long, potentially full-length cDNAs with restricted expression in anatomically complex regions of the brain. This protocol combining several existing methodologies is suitable for use with limited tissue sources and uses minimal amounts of isotopes.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Wang X,Brownstein MJ,Young WSdoi
10.1016/s0165-0270(97)02237-1subject
Has Abstractpub_date
1997-05-16 00:00:00pages
187-91issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(97)02237-1journal_volume
73pub_type
杂志文章abstract：BACKGROUND:Zebrafish (Danio rerio) are growing in popularity as a vertebrate model organism for the study of spinal neurocircuitry and locomotion. While many studies have used the zebrafish model system for electrophysiological analyses in embryonic and larval stages, there is a growing interest in studying spinal circ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.04.011
更新日期：2019-07-01 00:00:00
abstract：:We examined topographic variations in nonlinear measures based on scalp voltages, which were generated by two simulated current dipoles each placed in a different hemisphere of a spherical volume conductor (three-shell model). Dipole dynamics were that of a three-torus and the x-component of the Lorenz-system and scal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00188-6
更新日期：2000-05-15 00:00:00
abstract：:Many behavioural tests in rodents are based on the premise that basal locomotor activity of the animals is similar between the tested groups. The measurement of basal home cage activity is therefore an essential parameter, that should be included in all studies which employ tests of anxiety or cognition. Currently ava...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.01.008
更新日期：2007-05-15 00:00:00
abstract：:Electrophysiological recording provides important evidence for positive identification of many neurons in gastropods. We describe a technique which combines intracellular recording and injection of a persistent, non-fluorescent dye (Fast Green) with subsequent histofluorescence treatment using a modification of the wh...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90036-6
更新日期：1985-08-01 00:00:00
abstract：:Experimental recordings from neurons are generally difficult to quantify. Spike patterns and membrane potential cycles are often compared qualitatively and are hence limited by human resolution and judgement. This paper describes a statistical method for quantifying individual spike patterns and membrane potential flu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90053-t
更新日期：1993-10-01 00:00:00
abstract：:Skeletal muscle is a convenient model system for studying basic questions on the neural regulation of synaptogenesis and of many properties of sarcolemma and contractile apparatus. The study of the neural signals involved in a particular regulation and of the mediating intracellular pathways, requires the chronic appl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00352-6
更新日期：2001-04-30 00:00:00
abstract：:We have developed a novel, low-cost device designed to monitor and modulate locomotor activity in murine subjects. This technology has immediate application to the study of effects of physical exercise on various neurobiological endpoints, and will also likely be useful in the study of psychomotor sensitization and dr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.10.020
更新日期：2013-01-30 00:00:00
abstract：:The human movement analysis panel (HMAP) measures separable components of arm motion and simple and complex finger coordination. HMAP testing takes 30min to administer. In separate experiments we have validated the HMAP against the standard grooved pegboard and measures of gait speed, and demonstrated important learni...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.06.015
更新日期：2007-09-30 00:00:00
abstract：:In spinal cord injury (SCI) research, axon regeneration across spinal lesions is most often assessed using anatomical methods. It would be extremely advantageous, however, to examine the functional synaptic connectivity of regenerating fibres, using high-resolution electrophysiological methods. We have therefore devel...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.06.017
更新日期：2011-09-15 00:00:00
abstract：:Immunohistochemistry using antiserum against the basement membrane glycoprotein laminin, was shown to be an excellent marker for brain blood vessels. Throughout the brain of mice, rats, guinea pigs, monkeys and humans, the basement membrane of the vascular structures were strongly laminin-positive. The neuropil itself...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90128-7
更新日期：1986-09-01 00:00:00
abstract：:The aim of this simulation study was to investigate the influence of local tissue in-homogeneities on the estimates of muscle fiber conduction velocity (CV) from surface EMG signals. A recently developed analytical surface EMG model was used to generate simulated surface EMG signals from a planar layered volume conduc...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.07.011
更新日期：2005-03-15 00:00:00
abstract：BACKGROUND:The accuracy of source reconstruction depends on the spatial configuration of the neural sources underlying encephalographic signals, the temporal distance of the source activity, the level and structure of noise in the recordings, and - of course - on the employed inverse method. This plenitude of factors r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.02.016
更新日期：2018-05-01 00:00:00
abstract：:Recently, resting state functional connectivity (RSFC) studies based on fMRI and EEG/MEG have provided valuable insight into the intrinsic functional architecture of the human brain. However, whether functional near infrared spectroscopy (fNIRS), a suitable imaging method for infant and patient populations, can be use...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.11.010
更新日期：2010-02-15 00:00:00
abstract：:Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the rapid degradation of fatty acid amides such as the endocannabinoid anandamide. Inhibition of FAAH activity has been suggested as a therapeutic approach for the treatment of chronic pain, depression and anxiety, through local activation of the cannabin...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.10.006
更新日期：2007-03-30 00:00:00
abstract：:We used 24 sacral dorsal roots of the rat to analyze amplitude and area changes of biphasic and monophasic compound action potentials (CAPs) at 4 conduction distances. Both the CAPs of the A-fiber and C-fiber were analyzed. The changes were examined with the paired t test and linear regression. All the variables decre...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90064-x
更新日期：1993-03-01 00:00:00
abstract：:A solution is described for the acquisition on a personal computer of standard pulses derived from neuronal discharge, measurement of neuronal discharge times, real-time control of stimulus delivery based on specified inter-pulse interval conditions in the neuronal spike train, and on-line display and analysis of the ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00061-5
更新日期：1995-11-01 00:00:00
abstract：:A newly designed combination microelectrode-injection system is described which can be used to record electrophysiological responses of individual neurons in the primate brain to local administration of small quantities of drugs or other compounds. The assembly of the system is simple, and the materials used are inexp...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.03.015
更新日期：2004-09-30 00:00:00
abstract：BACKGROUND:Microelectrode arrays have been used successfully for neuronal stimulation both in vivo and in vitro. However, in most instances currents required to activate the neurons have been in un-physiological ranges resulting in neuronal damage and cell death. There is a need to develop electrodes which require less...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.024
更新日期：2015-06-15 00:00:00
abstract：:A new simple technique for the accurate modification of microelectrode and micropipette tips by breaking has been developed. The breaking is done by longitudinally inserting a micropipette into a larger previously broken pipette for stability and by applying a force on the micropipette to cause it to break. Tips will ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90106-1
更新日期：1983-09-01 00:00:00
abstract：BACKGROUND:Currently, behavioral research of aggressiveness is often conducted with intraspecific intermale aggression tests. Intraspecific aggression is not detectable in early stages of psychiatric disorders or in female animals, except during the nursing period. NEW METHOD:We developed a semi-automated apparatus (A...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.02.017
更新日期：2014-05-15 00:00:00
abstract：:The objective of visual systems neuroscience has shifted over the past few years from determining the receptive fields of cells towards the understanding of higher level cognition in awake animals viewing natural stimuli. In experiments with awake animals it is important to control the relevant aspects of behavior. Mo...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00423-x
更新日期：2001-09-30 00:00:00
abstract：:The objective of the present work was to examine fronto-central spindle frequency. A previously validated spindle detector, providing an electroencephalographic (EEG) amplitude independent spindle detection, was used to detect bilateral sleep spindles from sleep EEG recordings of ten healthy subjects with a time resol...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.03.019
更新日期：2008-07-15 00:00:00
abstract：:A method is described which allows simultaneous recording of the activity of several neurons in the visual cortex of awake unrestrained kittens for a period sufficiently long to follow experience-dependent changes in receptive field properties. The electrodes consisted of 25 microns thick Teflon-coated platinum-iridiu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90131-8
更新日期：1988-11-01 00:00:00
abstract：:Songbirds are the preeminent animal model for understanding how the brain encodes and produces learned vocalizations. Here, we report a new statistical method, the Kullback-Leibler (K-L) distance, for analyzing vocal change over time. First, we use a computerized recording system to capture all song syllables produced...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.06.033
更新日期：2008-09-15 00:00:00
abstract：BACKGROUND:Previous studies have measured whisker movements and locomotion to characterise mouse models of neurodegenerative disease. However, these studies have always been completed in isolation, and do not involve standardized procedures for comparisons across multiple mouse models and background strains. NEW METHO...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2019.108532
更新日期：2020-02-01 00:00:00
abstract：:The properties of 4 types of carbon electrodes designed for use as in vivo sensors of easily oxidized species in the mammalian brain have been evaluated in aqueous solutions at physiological pH. The electrodes are formed from a graphite-epoxy mixture, carbon paste, or carbon fibers, and have the geometries of a disk o...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90058-x
更新日期：1984-03-01 00:00:00
abstract：:Typically small animal radiological images are obtained after placing the animal in the center of the imaging device using beds or platforms, and then adjusting the position after obtaining a scout image. Such a process does not permit the reproducible visualization of the same anatomical plane with repeated examinati...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.01.003
更新日期：2004-06-15 00:00:00
abstract：:Practical aspects on the use of horseradish peroxidase (HRP) and wheat germ agglutinin-horseradish peroxidase conjugate (WGA-HRP) to trace peripheral cutaneous nerve endings have been studied. The parameters studied included application site of the tracer, post-application survival time, tracer concentration and trace...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90141-x
更新日期：1986-02-01 00:00:00
abstract：:Several neuronal tracing substances were applied to the cut ends of leech cephalic nerves and the resulting backfills into the subesophageal ganglion (sbEG) were mapped. A 12 h incubation in 3 kDa dextrans conjugated either to a fluorochrome or to biotin (subsequently tagged with peroxidase) was satisfactory. In separ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：BACKGROUND:The sound fed to a loudspeaker may significantly differ from that reaching the ear of the listener. The transformation from one to the other consists of spectral distortions with strong dependence on the relative locations of the speaker and the listener as well as on the geometry of the environment. With th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.08.025
更新日期：2018-11-01 00:00:00
