Abstract：
:In spinal cord injury (SCI) research, axon regeneration across spinal lesions is most often assessed using anatomical methods. It would be extremely advantageous, however, to examine the functional synaptic connectivity of regenerating fibres, using high-resolution electrophysiological methods. We have therefore developed a mouse horizontal spinal cord slice preparation that permits detailed analysis of evoked dorsal column (DCol) synaptic inputs on spinal neurons, using whole-cell patch clamp electrophysiology. This preparation allows us to characterise postsynaptic currents and potentials in response to electrical stimulation of DCol fibres, along with the intrinsic properties of spinal neurons. In addition, we demonstrate that low magnification calcium imaging can be used effectively to survey the spread of excitation from DCol stimulation in horizontal slices. This preparation is a potentially valuable tool for SCI research where confirmation of regenerated, functional synapses across a spinal lesion is critical.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Flynn JR,Brichta AM,Galea MP,Callister RJ,Graham BAdoi
10.1016/j.jneumeth.2011.06.017subject
Has Abstractpub_date
2011-09-15 00:00:00pages
113-20issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(11)00352-9journal_volume
200pub_type
杂志文章abstract：:The feasibility of spindle afferent recording during naturally or electrically induced hindlimb movements is demonstrated in the case of spinalized, decapitated turtles. Since these recordings are obtained from fine filaments of exposed dorsal roots, it is possible for the experimenter to select the afferents of inter...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90144-5
更新日期：1986-02-01 00:00:00
abstract：:This paper describes a computer program, named ASAP (audio sequencing and presentation), that runs on IBM PC compatible hardware with a Microsoft Windows operating system and has been specially designed for facilitating auditory neuroimaging studies. The program's main characteristics are: (1) the ability to generate ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00249-1
更新日期：2000-08-15 00:00:00
abstract：BACKGROUND:Anatomically and physiologically, there is strong relationship between the brain and body. A new MRI platform covering both the brain and the limb would be beneficial for a more thorough understanding of the brain-body interactions. NEW METHOD:A new arm-over-head (AOH) position was developed to collect MRI ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108829
更新日期：2020-10-01 00:00:00
abstract：:A method for quantifying nociceptive withdrawal reflex receptive fields in human volunteers and patients is described. The reflex receptive field (RRF) for a specific muscle denotes the cutaneous area from which a muscle contraction can be evoked by a nociceptive stimulus. The method is based on random stimulations pr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.11.009
更新日期：2009-03-30 00:00:00
abstract：:The most commonly used method for specifying the locations of functional areas in the human cerebral cortex is the coordinate system of Talairach and Tournoux (Co-planar Stereotaxic Altas of The Human Brain (1988) Georg Thieme Verlag, Stuttgart). It was designed to locate subcortical nuclei by reference to an axis joi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00046-3
更新日期：2003-05-30 00:00:00
abstract：:Two-photon laser scan microscopy carries many advantages for work on brain slices and bulk tissue. However, it has very low signal levels compared to conventional fluorescence microscopy. This is disadvantageous in fast imaging applications when photon shot noise is limiting. Working on brain slices with excitation po...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00103-x
更新日期：1999-10-15 00:00:00
abstract：:We have designed a device for long-term head fixation for use in behaving nonhuman primates that is robust yet minimally invasive and simple to use. This device is a modified version of the halo system that is used in humans for cervical traction and stabilization after spinal column injuries. This device consists of ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.04.012
更新日期：2009-06-30 00:00:00
abstract：BACKGROUND:There is a growing need for analyzing medical data such as brain connectomes. However, the unavailability of large-scale training samples increases risks of model over-fitting. Recently, deep learning (DL) architectures quickly gained momentum in synthesizing medical data. However, such frameworks are primar...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108988
更新日期：2020-11-04 00:00:00
abstract：:The most direct evaluation of human brain activity has been obtained from intracranial electrodes placed either on the surface of the brain or inserted into the brain to record from deep brain structures. Currently, the placement of intracranial electrodes implies transcranial surgery, either through a burr hole or a ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.12.027
更新日期：2013-03-30 00:00:00
abstract：:The use of c-fos protein (Fos) immunocytochemistry as a metabolic marker for tracing neuroanatomical connections, seizure pathways and sites of action of neuroactive drugs is discussed in this report. Fos immunocytochemistry will be very useful for these purposes providing that a number of potential problems are recog...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/0165-0270(89)90150-7
更新日期：1989-09-01 00:00:00
abstract：:We have developed a novel, low-cost device designed to monitor and modulate locomotor activity in murine subjects. This technology has immediate application to the study of effects of physical exercise on various neurobiological endpoints, and will also likely be useful in the study of psychomotor sensitization and dr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.10.020
更新日期：2013-01-30 00:00:00
abstract：:We describe an immunocytochemical method for marking microelectrode tracks made during single-unit recordings in long surviving, awake monkeys. This procedure detects the increase in glial fibrillary acidic protein in the glial cells along a microelectrode track using commercially available antibodies. We have success...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90085-r
更新日期：1992-03-01 00:00:00
abstract：:According to the amyloid hypothesis, Abeta peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Abeta clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Abeta peptide levels. The insulin-degrad...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.11.010
更新日期：2009-03-30 00:00:00
abstract：:This paper describes a simple multichannel hook electrode specially adapted for simultaneous recording from several dorsal root filaments in the cat. The electrode consists of a 3-mm-thick PVC plate and accommodates 12 silver wire hooks placed in notches in the plate. It has a semicircular shape, and the notches are m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)90058-2
更新日期：1994-04-01 00:00:00
abstract：:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90045-3
更新日期：1988-01-01 00:00:00
abstract：:We have adapted techniques for studying the locomotion of tethered insects to analysis of rapid directional movements such as escape behavior. We describe here a computer-based motion tracking system that allows an animal to turn and run as rapidly as it does under free-ranging conditions, and that samples fast enough...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00013-k
更新日期：1995-08-01 00:00:00
abstract：:A critical problem in neuroscience is the lack of database tools for integrating neuronal property data. We report here the development of a combined object oriented-relational database (NeuronDB, http://senselab.med.yale.edu/neurondb) that meets these needs by providing tools for integrating data within neurons and c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00049-1
更新日期：1998-07-01 00:00:00
abstract：:Recently there has been an increase in the use of spectral methods for the analysis of experimental data. These analytical methods allow the study of interactions between simultaneously recorded signals and are particularly suited to the study of systems displaying rhythmic behaviour. A useful parameter in this contex...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)02214-5
更新日期：1997-04-25 00:00:00
abstract：:A microthermistor positioned close to the exposed posterior semicircular canal in isolated labyrinth preparations of the frog was used to stimulate the sensory organ. Our results indicated that, depending on the position of the heater, the induced endolymphatic convection currents may result in either excitatory or in...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00021-7
更新日期：1999-05-01 00:00:00
abstract：:Zebra finches have been widely used to study neurobiology underlying vocal development. Because only male zebra finches learn song, efficient developmental use of these animals requires early determination of sex at ages that precede maturation of secondary sex characteristics. We have developed a sex determination me...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.04.007
更新日期：2007-08-15 00:00:00
abstract：:Many types of mazes have been used in cognitive brain research and data obtained from those experiments, especially those from rodents' studies, support the idea that the hippocampus is related to spatial learning and memory. But the results from non-human primates researches regarding the role of the hippocampus in s...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.12.017
更新日期：2008-05-15 00:00:00
abstract：:A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-β-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.09.016
更新日期：2010-12-15 00:00:00
abstract：BACKGROUND:Extraneural cuffs are among the least invasive peripheral nerve interfaces as they remain outside the nerve. However, compared with more invasive interfaces, these electrodes may suffer from lower selectivity and sensitivity since the targeted nerve fibers are more distanced from the electrodes. NEW METHOD:...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108634
更新日期：2020-04-15 00:00:00
abstract：:We describe a modification of the Harper-McGinty microdrive for use with extracellular, microwire recording in conscious, chronically prepared rabbits. Improvements over existing designs are in ease of assembly and mechanical stability. The microdrive is easily fabricated with hand tools and readily available parts. I...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.10.020
更新日期：2006-06-15 00:00:00
abstract：:The tail-skin temperature is an important factor in determining tail-flick latency to noxious radiant heat in rats and mice. A simple, non-invasive method for recording the tail-skin temperature during conventional tail-flick testing is described. The method is conveniently performed during conventional tail-flick tes...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90124-6
更新日期：1989-01-01 00:00:00
abstract：:Light emitting diodes (LEDs) are extensively used as light sources to investigate visual and visually related function and dysfunction. Here, we describe the design of a compact, low-cost, stand-alone LED-based system that enables the configuration, storage and presentation of elaborate visual stimulation paradigms. T...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.02.004
更新日期：2011-04-15 00:00:00
abstract：:Proteomic studies offer enormous potential for gaining insight into cellular dynamics and disease processes. An immediate challenge for enhancing the utility of proteomics in translational research lies in methods of handling and interpreting the large datasets generated. Publications rarely extend beyond lists of pro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.03.013
更新日期：2009-06-15 00:00:00
abstract：:Facial expressions of emotions are important in nonverbal communication. Although numerous neural structures have been identified to be involved in emotional face processing, the amygdala is thought to be a core moderator. While previous studies have relied on facial images of humans, the present study is concerned wi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.10.016
更新日期：2007-03-30 00:00:00
abstract：:Measurements of neurotransmitters in conscious animals have been restrictive in real-time. The use of specific enzymes within an amperometric probe, based upon a microdialysis membrane, may overcome some of these problems. I report the use of such a probe, with different perfusions of enzymes, to allow real-time measu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)02175-9
更新日期：1997-04-04 00:00:00
abstract：:Triphasic electrical stimulation pulses with an adjustable phase amplitude ratio (PAR) can reduce stimulus artifacts in electrically evoked compound actions potentials (ECAPs) recording in the cochlea (see companion paper Bahmer and Baumann, submitted for publication). The present study describes the application of tr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.12.006
更新日期：2012-03-30 00:00:00
