Abstract：
:According to the amyloid hypothesis, Abeta peptides are neurotoxic and underlie development and progression of Alzheimer's disease (AD). Multiple Abeta clearance mechanisms, including destruction of the peptides by proteolytic enzymes, are hypothesized to regulate physiological Abeta peptide levels. The insulin-degrading enzyme (IDE) is considered one of the predominant enzymes having Abeta degrading activity. Despite its putative role in protecting against AD, relatively few methods exist for studying IDE activity in vitro. We report the application of capillary electrophoresis (CE) as a novel method for evaluating IDE-mediated Abeta 1-40 proteolysis. This method employs chemically unmodified substrates that are readily obtained from commercial sources. It involves minimal sample preparation, and requires no specialized equipment beyond a CE instrument equipped with a standard fused silica capillary. In the present analysis, we demonstrate that this CE-based method is amenable to kinetic analysis, and show that IDE-mediated Abeta proteolysis is significantly and disproportionately inhibited in the presence of insulin, an alternative IDE substrate.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Alper BJ,Schmidt WKdoi
10.1016/j.jneumeth.2008.11.010subject
Has Abstractpub_date
2009-03-30 00:00:00pages
40-5issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(08)00664-Xjournal_volume
178pub_type
杂志文章abstract：BACKGROUND:Frequency domain Granger causality measures have been proposed and widely applied in analyzing rhythmic neurophysiological and biomedical signals. Almost all these measures are based on linear time domain regression models, and therefore can only detect linear causal effects in the frequency domain. NEW MET...
journal_title：Journal of neuroscience methods
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更新日期：2014-03-30 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.030
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.06.011
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.12.006
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.04.019
更新日期：2018-07-01 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.10.023
更新日期：2006-06-15 00:00:00
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journal_title：Journal of neuroscience methods
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更新日期：1994-03-01 00:00:00
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更新日期：2020-01-09 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.04.010
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.07.015
更新日期：2008-09-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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