Abstract：
:A method is described which allows simultaneous recording of the activity of several neurons in the visual cortex of awake unrestrained kittens for a period sufficiently long to follow experience-dependent changes in receptive field properties. The electrodes consisted of 25 microns thick Teflon-coated platinum-iridium wires whose tips were tapered and coated with platinum black. They were implanted individually into the striate cortex and fixed with tissue glue to the pia mater. The other end of the electrodes was connected to a 21-pin plug and fixed to the skull in a way which allowed the wires to follow freely any movements of the brain. With this arrangement single and multi unit activity could be recorded from up to 10 sites over several weeks. In fortuitous cases the activity of a single cell could be followed over several days. The receptive field properties of these neurons were determined in awake animals by computing response histograms to moving gratings that covered the whole visual field and whose orientation could be changed in small steps. Comparison with receptive field properties that were determined for the same neurons after the kittens had been lightly anesthetized, revealed that this method allowed reliable determination of the eye preference of the neurons as well as of their orientation and direction selectivity. Since this method allowed repeated non-invasive measurements of neuronal response properties in only lightly restrained kittens, it was possible to study, in individual units, the nature and time course of changes in receptive field properties such as result from monocular deprivation and reverse occlusion.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Mioche L,Singer Wdoi
10.1016/0165-0270(88)90131-8subject
Has Abstractpub_date
1988-11-01 00:00:00pages
83-94issue
1eissn
0165-0270issn
1872-678Xpii
0165-0270(88)90131-8journal_volume
26pub_type
杂志文章abstract：:A system for autoregulation of body temperature of large and small animals is described. The device uses the IC AD590 as a temperature transducer. It operates on the basis of continuous regulation of the heating current, and does not emit transients which interfere with electrical recordings. A panel meter shows eithe...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90128-3
更新日期：1989-12-01 00:00:00
abstract：BACKGROUND:Fear conditioning (FC) in rodents is the most used animal model to investigate the neurobiology of posttraumatic stress disorder (PTSD). Although research using FC has generated a better understanding of fear memories, studies often rely on mild or moderate FC training and behavioral analysis generally focus...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.02.004
更新日期：2018-03-15 00:00:00
abstract：:The relationship between pressure, ejection duration and volume ejected was experimentally determined in vitro for micropipettes with different external tip diameters. The relationship between ejection duration and ejected volume is linear in the steady state (i.e. with ejection durations of 1 s or longer) and at suff...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90021-9
更新日期：1984-04-01 00:00:00
abstract：BACKGROUND:Magnetoencephalography (MEG) and Electroencephalography (EEG) are noninvasive techniques to study the electrophysiological activity of the human brain. Thus, they are well suited for real-time monitoring and analysis of neuronal activity. Real-time MEG/EEG data processing allows adjustment of the stimuli to ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.03.020
更新日期：2018-06-01 00:00:00
abstract：:Previous neuroimaging studies of the cortical representation of gustatory and olfactory stimuli have often delivered tastants to the mouth in very small quantities or stimulated olfaction orthonasally. In studies of retro-nasal olfaction, swallowing was generally delayed to reduce head motion artefacts. The present fM...
journal_title：Journal of neuroscience methods
pub_type： 临床试验,杂志文章
doi：10.1016/j.jneumeth.2006.05.035
更新日期：2006-12-15 00:00:00
abstract：:There is increasing evidence that oxidative stress plays an important role in the pathogenesis of many neurodegenerative diseases including Parkinson's disease (PD). In particular there is support for the participation of oxidized catecholamines in PD. Catecholamines are highly reactive and are readily oxidized to ami...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.08.010
更新日期：2005-03-30 00:00:00
abstract：:Modern clearing techniques enable high resolution visualization and 3D reconstruction of cell populations and their structural details throughout large biological samples, including intact organs and even entire organisms. In the past decade, these methods have become more tractable and are now being utilized to provi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2020.108594
更新日期：2020-01-13 00:00:00
abstract：BACKGROUND:Current neuronal monitoring techniques, such as calcium imaging and multi-electrode arrays, enable recordings of spiking activity from hundreds of neurons simultaneously. Of primary importance in systems neuroscience is the identification of cell assemblies: groups of neurons that cooperate in some form with...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.08.011
更新日期：2014-10-30 00:00:00
abstract：BACKGROUND:The input signals of electroencephalography (EEG) based brain computer interfaces (BCI) are extensively acquired from scalp with a multi-channel system. However, multi-channel signals might contain redundant information and increase computational complexity. Furthermore, using only effective channels, rather...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.12.004
更新日期：2019-02-01 00:00:00
abstract：:We describe a novel method for retrogradely labeling specific neuronal populations using voltage-sensitive dyes. Styryl dyes were injected into the ventral roots of the isolated embryonic chick spinal cord. After waiting several hours, the dye labeled motoneurons and autonomic preganglionic neurons. Neuronal cell bodi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)00108-2
更新日期：1996-12-28 00:00:00
abstract：:Single, i.e. unconnected spinal or cerebral neurons from chick embryos rapidly regenerate morphologically identifiable axons and dendrites when cultured in a three-dimensional (3-D) extracellular matrix (ECM) consisting of a hydrated native collagen lattice. We now show that it is possible to study the intrinsic elect...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90052-5
更新日期：1987-07-01 00:00:00
abstract：BACKGROUND:Sholl analysis has been used to analyze neuronal morphometry and dendritic branching and complexity for many years. While the process has become semi-automated in recent years, existing software packages are still dependent on user tracing and hence are subject to observer bias, variability, and increased us...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108529
更新日期：2020-02-01 00:00:00
abstract：:Frequency analyses of EEG data yield large data sets, which are high-dimensional and have to be evaluated statistically without a large number of false positive statements. There exist several methods to deal with this problem in multiple comparisons. Knowing the number of true hypotheses increases the power of some m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.12.013
更新日期：2008-05-15 00:00:00
abstract：:Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.09.034
更新日期：2009-02-15 00:00:00
abstract：:Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common genetic cause of Parkinson's disease. LRRK2 kinase activity is required for toxicity in neuronal cell cultures suggesting that selective kinase inhibitors may prevent neurodegeneration in patients. Directly monitoring LRRK2 activity in cells would b...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.12.015
更新日期：2013-03-30 00:00:00
abstract：:Isotropic fractionation is a quantitative technique that allows reliable estimates of absolute numbers of neuronal and non-neuronal brain cells. However, being fast for single small brains, it requires a long time for processing large brains or many small ones, if done manually. To solve this problem, we developed a m...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.09.015
更新日期：2013-01-15 00:00:00
abstract：:Isolated turtle brain/eye preparation has recently been used as a bloodless animal model for detecting the magnetic resonance imaging (MRI) signal changes produced by visually evoked neuronal currents. The present work aims to determine whether checkerboard-patterned or full field flash (blank) stimulation should be u...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.12.005
更新日期：2010-03-15 00:00:00
abstract：:The design of a simple and inexpensive battery powered lamp controller for microscope lamps commonly used in patch clamp and other biophysical studies is described. The circuit linearizes the normally exponential voltage-intensity curve of standard 12-V lamps by using an operational amplifier to provide feedback to th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90098-k
更新日期：1991-10-01 00:00:00
abstract：:We present a numerical method which provides the ability to analyze digitized microscope images of retinal explants and quantify neurite outgrowth. Few parameters are required as input and limited user interaction is necessary to process an entire experiment of images. This eliminates fatigue related errors and user-r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00017-7
更新日期：2003-04-15 00:00:00
abstract：:The reproducibility of motor unit twitches obtained using spike-triggered averaging (STA) was examined in the human first dorsal interosseus. For each motor unit (30 total) a series of STA twitches was derived using a 30 s averaging window. Within each averaging window, eight independent measures characterizing motor ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00077-3
更新日期：2003-06-30 00:00:00
abstract：BACKGROUND:This article addresses the automatic classification of reconstructed neurons through their morphological features. The purpose was to extend the capabilities of the L-Measure software. METHODS:New morphological features were developed, based on modifications of the conventional Sholl analysis. The lengths o...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108835
更新日期：2020-09-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90002-x
更新日期：1983-03-01 00:00:00
abstract：BACKGROUND:With advances in cell capture, gene expression can now be studied in neuronal subtypes and single cells; however, studying epigenetic mechanisms that underlie these changes presents challenges. Moreover, chromatin immunoprecipitation (ChIP) protocols optimized for low cell number do not adequately address te...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.02.006
更新日期：2016-04-01 00:00:00
abstract：:Ischemia induces a 'blebbing' of dendrites, a structural alteration where dendrites take on a 'beads on a string' appearance. We developed a toolkit program, BlebQuant, for quantitative automated bleb analysis to chart the morphology of dendrites labeled with GFP/YFP under normal conditions and after ischemia-induced ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.12.018
更新日期：2011-02-15 00:00:00
abstract：BACKGROUND:Accurate and reliable measurement of leukoaraiosis, or MR-detected white, matter hyper-intensity (WMH) burden in subjects with acute ischemic stroke (AIS) is important for, ongoing research studies and future models of risk and outcome prediction, but the presence of a, cerebral infarct may complicate measur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.10.009
更新日期：2014-01-15 00:00:00
abstract：:The coupling of Ca(2+) influx to synaptic vesicle (SV) recycling in nerve terminals is essential for neurotransmitter release and thus neuronal communication. Both of these parameters have been monitored using fluorescent reporter dyes such as fura-2 and FM1-43 in single central nerve terminals. However, their simulta...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.09.012
更新日期：2007-03-15 00:00:00
abstract：BACKGROUND:Planarians, like rodents, instinctively spend more time in dark versus light environments when given a choice. This behavioral phenomenon is called negative phototaxis, which may reflect defensive responding related to an anxiety-like phenotype. NEW METHOD:We propose a planarian light/dark test, designated ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.10.010
更新日期：2018-01-01 00:00:00
abstract：:A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been established for the rapid and reliable determination of gamma-aminobutyric acid (GABA) and glutamate in brain microdialysates. The microdialysis samples were analysed using a HILIC (hydrophilic interaction liquid chromatography) column, which ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.05.018
更新日期：2009-08-30 00:00:00
abstract：:Four different fluorochromes were injected into adjacent cervical spinal cord segments, 1 unique tracer per segment. Each tracer, Fluoro-Gold, Fast Blue, Diamidino Yellow dihydrochloride and Propidium Iodide, was taken up by axonal terminals and transported intra-axonally in a retrograde direction to the cell bodies. ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90039-g
更新日期：1992-10-01 00:00:00
abstract：:Chimera construction between different proteins is a useful method for investigating protein structure and function relationships. However, this technique, by its traditional application, has been daunting because of its complex procedure and low success rate. Here we describe a protocol for constructing chimeras betw...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.08.033
更新日期：2010-10-30 00:00:00
