Abstract:
:We present an effective technique for culture and expansion of Schwann cells (SC) from adult peripheral nerves. Cultures from adult mouse sciatic nerves (one to six nerves per culture) in defined medium showed markedly higher purity and density of SC when the nerve was predegenerated in vivo for 7 days than when it was harvested fresh. SC from degenerated nerves were then cultured in defined media conditioned by primary cultures of adult SC. The best results were obtained with a conditioned medium supplemented with 1% fetal calf serum. In these conditions the purity of SC was about 90% and the density about 190 cell/mm(2) by 7-10 days in vitro. These findings indicate that adult SC can be expanded from small preinjured nerve fragments in a short time period to provide a source of SC for autologous cellular transplants.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Verdú E,Rodríguez FJ,Gudiño-Cabrera G,Nieto-Sampedro M,Navarro Xdoi
10.1016/s0165-0270(00)00221-1subject
Has Abstractpub_date
2000-06-30 00:00:00pages
111-7issue
1-2eissn
0165-0270issn
1872-678Xpii
S0165027000002211journal_volume
99pub_type
杂志文章abstract::Thermal laser stimulation of the skin is an efficient exploratory tool to characterize the nociceptive system. In the present study, finite element simulations are done to calculate the intra-cutaneous spatio-temporal temperature profiles following the delivery of such laser stimuli. The proposed computer-aided modeli...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.11.010
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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