Expansion of adult Schwann cells from mouse predegenerated peripheral nerves.

Abstract:

:We present an effective technique for culture and expansion of Schwann cells (SC) from adult peripheral nerves. Cultures from adult mouse sciatic nerves (one to six nerves per culture) in defined medium showed markedly higher purity and density of SC when the nerve was predegenerated in vivo for 7 days than when it was harvested fresh. SC from degenerated nerves were then cultured in defined media conditioned by primary cultures of adult SC. The best results were obtained with a conditioned medium supplemented with 1% fetal calf serum. In these conditions the purity of SC was about 90% and the density about 190 cell/mm(2) by 7-10 days in vitro. These findings indicate that adult SC can be expanded from small preinjured nerve fragments in a short time period to provide a source of SC for autologous cellular transplants.

journal_name

J Neurosci Methods

authors

Verdú E,Rodríguez FJ,Gudiño-Cabrera G,Nieto-Sampedro M,Navarro X

doi

10.1016/s0165-0270(00)00221-1

subject

Has Abstract

pub_date

2000-06-30 00:00:00

pages

111-7

issue

1-2

eissn

0165-0270

issn

1872-678X

pii

S0165027000002211

journal_volume

99

pub_type

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