当前位置： SCI文献检索 > JOURNAL OF NEUROSCIENCE METHODS期刊下所有文献 > Fast transdifferentiation of human Wharton's jelly mesenchymal stem cells into neurospheres and nerve-like cells.

Fast transdifferentiation of human Wharton's jelly mesenchymal stem cells into neurospheres and nerve-like cells.

Abstract：

BACKGROUND:The human mesenchymal stem cells derived from Wharton's jelly tissue (hWJ-MSCs) represent a tool for cell-based therapies and regenerative medicine. hWJ-MSCs form neurospheres (NSs) within 3-7 days. No data is available to establish the neuro-phenotypic markers and time of formation of nerve-like (NLCs) and glial cells from NSs derived from hWJ-MSCs. NEW METHOD: hWJ-MSCs were incubated with Fast-N-Spheres medium for 24 and 72h. The new formed NSs were in turn incubated with forskolin in neurogenic NeuroForsk medium for 1-7days. RESULTS:hWJ-MSCs cultured with Fast-N-Spheres medium trans-differentiated into NSs in just 24h compared to 72h for hWJ-MSCs cultured with classic growth factor medium. The NSs generated from the Fast-N-Spheres medium expressed reduced levels SOX2, OCT4 and NANOG, as markers of pluripotency compared to undifferentiated hWJ-MSCs. The formed NSs exposed to NeuroForsk medium differentiated into NLCs in 4days as evidenced by high levels of protein expression of the neuronal markers, and no expression of the glial marker GFAP. COMPARISON WITH EXISTING METHOD(S):Currently, the formation and harvest of NSs is expensive and time consuming. Published protocols require 3-7days to form NSs from whole human umbilical cord MSCs. We report for the first time, to our knowledge, the differentiation of NSs-derived from hWJ-MSCs into NLCs. CONCLUSIONS:The fastest method to obtain NSs and NLCs from hWJ-MSCs takes only five days using the two-step incubation media Fast-N-Spheres and NeuroForsk.

journal_name

J Neurosci Methods

journal_title

Journal of neuroscience methods

authors

Bonilla-Porras AR,Velez-Pardo C,Jimenez-Del-Rio M

doi

10.1016/j.jneumeth.2017.03.005

subject

Has Abstract

pub_date

2017-04-15 00:00:00

pages

52-60

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(17)30062-6

journal_volume

282

pub_type

杂志文章

相关文献

JOURNAL OF NEUROSCIENCE METHODS文献大全
  • Threshold depolarization measurements in resting human motoneurones.

    abstract：:Threshold depolarization is defined as the amplitude of the depolarization that will just bring a neurone to its firing threshold. Here, a novel technique is described for the measurement of threshold depolarization in resting human motoneurones. The tibial nerve was stimulated at an intensity that was just sufficient...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(91)90097-j

    authors： Türker KS,Miles TS

    更新日期：1991-08-01 00:00:00

  • Acute in vivo testing of a polymer cuff electrode with integrated microfluidic channels for stimulation, recording, and drug delivery on rat sciatic nerve.

    abstract：BACKGROUND:Extraneural cuffs are among the least invasive peripheral nerve interfaces as they remain outside the nerve. However, compared with more invasive interfaces, these electrodes may suffer from lower selectivity and sensitivity since the targeted nerve fibers are more distanced from the electrodes. NEW METHOD:...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2020.108634

    authors： Elyahoodayan S,Larson C,Cobo AM,Meng E,Song D

    更新日期：2020-04-15 00:00:00

  • Techniques for improving stereotaxic accuracy in Macaca fascicularis.

    abstract：:Sources of stereotaxic variability in long-tailed macaques (Macaca fascicularis) were examined by X-ray techniques. Accuracy in the vertical dimension can be improved significantly by measuring from the top of the brain rather than from the Frankfurt plane established by external bony landmarks. Even greater accuracy ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(85)90029-9

    authors： Dubach MF,Tongen VC,Bowden DM

    更新日期：1985-04-01 00:00:00

  • The use of social interaction as a method for detecting anxiolytic activity of chlordiazepoxide-like drugs.

    abstract：:The social interaction test in rats provides a method for detecting anxiolytic activity that does not use food or water deprivation, or electric shock, and therefore obviates difficulties of interpretation that might arise from drug-induced changes in motivation. Since social interaction is measured under more than on...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(80)90012-6

    authors： File SE

    更新日期：1980-06-01 00:00:00

  • A symmetrical Waxholm canonical mouse brain for NeuroMaps.

    abstract：:NeuroMaps (2010) is a Web-based application that enables investigators to map data from macaque studies to a canonical atlas of the macaque brain. It currently serves as an image processor enabling them to create figures suitable for publication, presentation and archival purposes. Eventually it will enable investigat...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2010.11.028

    authors： Bowden DM,Johnson GA,Zaborsky L,Green WD,Moore E,Badea A,Dubach MF,Bookstein FL

    更新日期：2011-02-15 00:00:00

  • Frequency-based approach to the study of semantic brain networks connectivity.

    abstract：:The interactions among cerebral regions involved in semantic word generations are explored through connectivity analysis based on fMRI data through multivariate autoregressive model (MVAR). Connections among the pars triangularis of the left inferior frontal gyrus (L45), the lateral fusiform girus (LFG) and the left m...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2012.10.005

    authors： Bianchi AM,Marchetta E,Tana MG,Tettamanti M,Rizzo G

    更新日期：2013-01-30 00:00:00

  • Method of analysis of local neuronal circuits in the vertebrate central nervous system.

    abstract：:Although a considerable amount of knowledge has been accumulated about the activity of individual nerve cells in the brain, little is known about their mutual interactions at the local level. The method presented in this paper allows the reconstruction of functional relations within a group of neurons as recorded by a...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章,评审

    doi：10.1016/0165-0270(92)90061-h

    authors： Reinis S,Weiss DS,McGaraughty S,Tsoukatos J

    更新日期：1992-06-01 00:00:00

  • Diffuse sleep spindles show similar frequency in central and frontopolar positions.

    abstract：:The objective of the present work was to examine fronto-central spindle frequency. A previously validated spindle detector, providing an electroencephalographic (EEG) amplitude independent spindle detection, was used to detect bilateral sleep spindles from sleep EEG recordings of ten healthy subjects with a time resol...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2008.03.019

    authors： Huupponen E,Kulkas A,Tenhunen M,Saastamoinen A,Hasan J,Himanen SL

    更新日期：2008-07-15 00:00:00

  • Accurate quantitative RT-PCR for relative expression of Slo splice variants.

    abstract：:Much interest has been shown in the use of multi-template reverse transcription-polymerase chain reaction (RT-PCR) as a quantitative instrument for low-abundance mRNAs. A desire to achieve finely-graded quantification of the stress- and hormone-related regulation of one splicing decision in an ion channel gene motivat...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(02)00015-8

    authors： Mahmoud SF,Bezzerides AL,Riba R,Lai GJ,Lovell PV,Hara Y,McCobb DP

    更新日期：2002-04-15 00:00:00

  • Recording of electroneurograms from the nerves innervating the pancreas of a dog.

    abstract：:Electroneurograms (ENGs) from the vagus, splanchnic and pancreatic nerves innervating the pancreas of a dog, were recorded with chronically implanted silicone multi-electrode circular cuffs in an intact pancreas and in a pancreas partly disabled with alloxan. The cuffs contained 33 platinum electrodes (0.6x1.5 mm) arr...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(01)00462-9

    authors： Rozman J,Zorko B,Bunc M

    更新日期：2001-12-15 00:00:00

  • Estimation of volume fractions in nervous tissue with an image analyzer.

    abstract：:The calculation of volume fractions in nervous tissue is a method often used in neuroanatomy. In ontogenetic studies and problems concerning aging, as well as after experimental procedures, volume fractions are calculated in order to quantify the results. Grey level index (GLI) and grey cell coefficient (GCC) are diff...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(82)90014-0

    authors： Wree A,Schleicher A,Zilles K

    更新日期：1982-07-01 00:00:00

  • Direct interaction with no correlation: an experimental pitfall in neural systems.

    abstract：:Neural systems continuously optimize how organisms process their environment and are highly dynamic. Building predictive models of these systems is challenging due to the large number of their elements. Therefore, in experimental and descriptive neurobiology the researcher typically does not seek to catalogue all vari...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2012.02.012

    authors： Janušonis S

    更新日期：2012-01-01 00:00:00

  • Rapid prototyping for neuroscience and neural engineering.

    abstract：:Rapid prototyping (RP) is a useful method for designing and fabricating a wide variety of devices used for neuroscience research. The present study confirms the utility of using fused deposition modeling, a specific form of RP, to produce three devices commonly used for basic science experimentation. The accuracy and ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2008.03.011

    authors： Tek P,Chiganos TC,Mohammed JS,Eddington DT,Fall CP,Ifft P,Rousche PJ

    更新日期：2008-07-30 00:00:00

  • A perfused in vitro preparation of hypothalamus for electrophysiological studies on neurosecretory neurons.

    abstract：:A technique for the preparation and recording of mammalian supraoptic nucleus neurosecretory neurons maintained in vitro through perfusion of the anterior cerebral artery is described. A slice of basal diencephalon measuring approximately 8 X 8 X 2 mm that includes the pituitary stalk and medial preoptic-anterior peri...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(83)90002-x

    authors： Bourque CW,Renaud LP

    更新日期：1983-03-01 00:00:00

  • l-Glutamate in Middlebrook 7H9 culture medium upregulates matrix metalloproteinase-2 secretion from human astrocytoma cells.

    abstract：:Matrix metalloproteinases (MMPs) are implicated in the pathology of CNS tuberculosis. Whilst investigating the secretion of MMP-2 from human U373-MG astrocytoma cells, we observed elevated MMP-2 secretion in response to Middlebrook 7H9 media but not to Mycobacterium tuberculosis itself. Middlebrook 7H9 media did not s...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2008.06.007

    authors： Harris JE,Friedland JS

    更新日期：2008-08-30 00:00:00

  • An initial validation of the Virtual Environment Grocery Store.

    abstract：BACKGROUND:Virtual reality-based neuropsychological assessments proffer the potential to address the limited ecological validity of pen-and-paper measures of memory. NEW METHOD:To investigate the construct validity of a newly developed virtual reality-based multiple errands task, the Virtual Environment Grocery Store ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2017.07.027

    authors： Parsons TD,McMahan T

    更新日期：2017-11-01 00:00:00

  • A lucite plate method for 3-dimensional reconstruction of neuronal populations.

    abstract：:A Lucite plate reconstruction method is described which, when combined with HRP histochemistry, provides an excellent means of visualizing, in 3-dimensional fashion, the functional organization of neuronal populations. Color photomicrographs of representative serial sections were made through the baboon oculomotor nuc...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(81)90019-4

    authors： Augustine JR

    更新日期：1981-06-01 00:00:00

  • A new approach to neural cell culture for long-term studies.

    abstract：:We have developed a new method for culturing cells that maintains their health and sterility for many months. Using conventional techniques, primary neuron cultures seldom survive more than 2 months. Increases in the osmotic strength of media due to evaporation are a large and underappreciated contributor to the gradu...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(01)00412-5

    authors： Potter SM,DeMarse TB

    更新日期：2001-09-30 00:00:00

  • Demarcation of secondary hyperalgesia zones: Punctate stimulation pressure matters.

    abstract：BACKGROUND:Secondary hyperalgesia is increased sensitivity in normal tissue near an injury, and it is a measure of central sensitization reflecting injury-related effects on the CNS. Secondary hyperalgesia areas (SHAs), usually assessed by polyamide monofilaments, are important outcomes in studies of analgesic drug eff...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章,随机对照试验

    doi：10.1016/j.jneumeth.2015.08.018

    authors： Ringsted TK,Enghuus C,Petersen MA,Werner MU

    更新日期：2015-12-30 00:00:00

  • Collection and handling of ultrathin serial sections for 3-dimensional reconstruction.

    abstract：:Serial sectioning for 3-D reconstruction requires a highly skilled and experienced individual to collect ribbons of ultrathin sections on formvar-coated grids, and to handle the grids after section collection. A simple method is described for placing ribbons in an orderly serial fashion on formvar-coated grids, by a m...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(89)90057-5

    authors： Wali N,Jagadeesh JM

    更新日期：1989-11-01 00:00:00

  • Progressive incorporation of propidium iodide in cultured mouse neurons correlates with declining electrophysiological status: a fluorescence scale of membrane integrity.

    abstract：:We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We corr...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(90)90007-3

    authors： Macklis JD,Madison RD

    更新日期：1990-01-01 00:00:00

  • Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays.

    abstract：:In order to identify novel genes involved in appetite and body weight regulation we have developed a microarray based method suitable for detecting small changes in gene expression in discrete groups of hypothalamic neurons. The method is based on a combination of stereological sampling, laser capture microdissection ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2008.09.024

    authors： Paulsen SJ,Larsen LK,Jelsing J,Janssen U,Gerstmayer B,Vrang N

    更新日期：2009-02-15 00:00:00

  • Purification of peptides derived from proenkephalin A on Bio-Rex 70.

    abstract：:The weakly acidic, cation-exchange resin Bio-Rex 70 was used at a low pH for chromatography of enkephalins and other peptides containing enkephalin sequences. Recoveries of various peptide standards and peptides in adrenal chromaffin cell extracts were greater than 85%. Catecholamines and other substances such as dens...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(85)90052-4

    authors： Wilson SP

    更新日期：1985-10-01 00:00:00

  • Noninvasive in vivo assessment of muscle impairment in the mdx mouse model--a comparison of two common wire hanging methods with two different results.

    abstract：:Duchenne muscular dystrophy (DMD) is an X-chromosome-linked disorder that arises from a mutation in the gene for the cytoskeletal protein dystrophin, normally expressed in the myofibres. The most widely applied animal model in DMD basic research is the C57BL/10ScSn-mdx/J mouse, commonly referred to as the "mdx mouse"....

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2011.10.001

    authors： Klein SM,Vykoukal J,Lechler P,Zeitler K,Gehmert S,Schreml S,Alt E,Bogdahn U,Prantl L

    更新日期：2012-01-30 00:00:00

  • Evaluation of neuronal numerical density by Dirichlet tessellation.

    abstract：:The technique that we describe aims at evaluating the numerical density of cells in highly heterogeneous regions, e.g., nuclei, layers or columns of neurones. Rather than counting the number of neuronal sections ('profiles') in a reference frame, we evaluated the 'free area' which lies around each profile. The X and Y...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(94)90025-6

    authors： Duyckaerts C,Godefroy G,Hauw JJ

    更新日期：1994-01-01 00:00:00

  • A wavelet based algorithm for the identification of oscillatory event-related potential components.

    abstract：:Event related potentials (ERPs) are very feeble alterations in the ongoing electroencephalogram (EEG) and their detection is a challenging problem. Based on the unique time-based parameters derived from wavelet coefficients and the asymmetry property of wavelets a novel algorithm to separate ERP components in single-t...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2014.06.004

    authors： Aniyan AK,Philip NS,Samar VJ,Desjardins JA,Segalowitz SJ

    更新日期：2014-08-15 00:00:00

  • In vivo modeling of prodromal stage of Parkinson's disease.

    abstract：:Parkinson's disease (PD) has a long prodromal period that precedes the appearance of typical motor symptoms. This phase is extremely heterogeneous and is characterized by a wide range of non-motor alterations including REM sleep behavior disorders, constipation, olfactory and urinary dysfunctions. The increasing clini...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章,评审

    doi：10.1016/j.jneumeth.2020.108801

    authors： Cerri S,Blandini F

    更新日期：2020-08-01 00:00:00

  • A microassay for simultaneous measurement of in vivo rates of tryptophan hydroxylation and levels of serotonin in discrete brain nuclei.

    abstract：:A sensitive procedure for simultaneous determination of in vivo rates of tryptophan hydroxylation and levels of serotonin (5-HT) in discrete rat brain nuclei is described. Rates of tryptophan hydroxylation are estimated by 5-hydroxytryptophan (5-HTP) accumulation following l-aromatic amino acid decarboxylase inhibitio...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(82)90015-2

    authors： Long JB,Youngblood WW,Kizer JS

    更新日期：1982-07-01 00:00:00

  • Cleaning MEG artifacts using external cues.

    abstract：:Using EEG, ECoG, MEG, and microelectrodes to record brain activity is prone to multiple artifacts. The main power line (mains line), video equipment, mechanical vibrations and activities outside the brain are the most common sources of artifacts. MEG amplitudes are low, and even small artifacts distort recordings. In ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2013.04.002

    authors： Tal I,Abeles M

    更新日期：2013-07-15 00:00:00

  • Locomotor activity measures in the diagnosis of attention deficit hyperactivity disorder: Meta-analyses and new findings.

    abstract：INTRODUCTION:Our aim was to assess differences in movement measures in attention-deficit/hyperactivity disorder (ADHD) vs. typically developing (TD) controls. METHODS:We performed meta-analyses of published studies on motion measures contrasting ADHD with controls. We also conducted a case-control study with children/...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.jneumeth.2015.03.001

    authors： García Murillo L,Cortese S,Anderson D,Di Martino A,Castellanos FX

    更新日期：2015-08-30 00:00:00