Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays.


:In order to identify novel genes involved in appetite and body weight regulation we have developed a microarray based method suitable for detecting small changes in gene expression in discrete groups of hypothalamic neurons. The method is based on a combination of stereological sampling, laser capture microdissection (LCM), PCR based amplification (SuperAmp), and one-color cDNA microarray analysis. To validate the method we assessed and compared fasting induced changes in mRNA levels of Neuropeptide Y (NPY) and proopiomelanocortin (POMC) in the hypothalamic arcuate nucleus (ARC) of diet-induced obese rats using cDNA microarrays, quantitative PCR and in situ hybridization. All methods revealed statistically significant fasting-induced changes in NPY and POMC expression. An additional 3480 differentially expressed probes (fold change >1.22, t-test p=0.05) were identified in the microarray analysis. Our findings demonstrate a consistent gene expression pattern across three different gene expression detection methods and strongly suggest that LCM coupled microarray analysis combined with SuperAmp can be used as a semi-quantitative mRNA profiling tool. Importantly, the sensitivity of the method greatly improves the usefulness of the microarray technology for gene expression profiling in non-homogeneous tissues such as the brain.


J Neurosci Methods


Paulsen SJ,Larsen LK,Jelsing J,Janssen U,Gerstmayer B,Vrang N




Has Abstract


2009-02-15 00:00:00














  • A white matter stroke model in the mouse: axonal damage, progenitor responses and MRI correlates.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Sozmen EG,Kolekar A,Havton LA,Carmichael ST

    更新日期:2009-06-15 00:00:00

  • A new reproducible focal cerebral ischemia model by introduction of polyvinylsiloxane into the middle cerebral artery: a comparison study.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Yang Y,Yang T,Li Q,Wang CX,Shuaib A

    更新日期:2002-08-30 00:00:00

  • Finite element analysis of thermal laser skin stimulation for a finer characterization of the nociceptive system.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Marchandise E,Mouraux A,Plaghki L,Henrotte F

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  • Complexity in differentiating the expression of truncated or matured forms of MMP-2 and MMP-9 through zymography in rat brain tissues after acute ischaemic stroke.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Alam M,Shuaib A

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  • Registration of in vivo magnetic resonance T1-weighted brain images to triphenyltetrazolium chloride stained sections in small animals.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Li G,Nikolova S,Bartha R

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  • iELVis: An open source MATLAB toolbox for localizing and visualizing human intracranial electrode data.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Groppe DM,Bickel S,Dykstra AR,Wang X,Mégevand P,Mercier MR,Lado FA,Mehta AD,Honey CJ

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  • Constructing fMRI connectivity networks: a whole brain functional parcellation method for node definition.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Electrokinetic infusions into hydrogels and brain tissue: Control of direction and magnitude of solute delivery.

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    journal_title:Journal of neuroscience methods

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    authors: Shan Q,Lynch JW

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hoshi K,Shingai R

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Eriksdotter-Nilsson M,Björklund H,Olson L

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    journal_title:Journal of neuroscience methods

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Herbst JA,Gammeter S,Ferrero D,Hahnloser RH

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Huang F,Li J,Shi HL,Wang TT,Muhtar W,Du M,Zhang BB,Wu H,Yang L,Hu ZB,Wu XJ

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kliem MA,Wichmann T

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hajosch R,Grupp L,Nichterwitz S,Schlosshauer B

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    authors: Borroto-Escuela DO,Correia PA,Romero-Fernandez W,Narvaez M,Fuxe K,Ciruela F,Garriga P

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    journal_title:Journal of neuroscience methods

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    authors: Ahemaiti A,Ainla A,Jeffries GD,Wigström H,Orwar O,Jesorka A,Jardemark K

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    journal_title:Journal of neuroscience methods

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    journal_title:Journal of neuroscience methods

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    journal_title:Journal of neuroscience methods

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Drago J,Murphy M,Bailey KA,Bartlett PF

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Muller LM,Kennedy JA

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Jaw FS,Tsao HW,Yu HJ,Yen CT

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Edström A,Sjöberg J,Kanje M

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  • Measurements of behavior in the naked mole-rat after intraperitoneal implantation of a radio-telemetry system.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Herold N,Spray S,Horn T,Henriksen SJ

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Gorter JA,van Vliet EA,Lopes da Silva FH

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  • Classification of methods in transcranial electrical stimulation (tES) and evolving strategy from historical approaches to contemporary innovations.

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    pub_type: 历史文章,杂志文章,评审


    authors: Guleyupoglu B,Schestatsky P,Edwards D,Fregni F,Bikson M

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Meier SD,Kovalchuk Y,Rose CR

    更新日期:2006-09-15 00:00:00