Abstract：
:The permanence of retrograde neuronal labeling by the fluorescent tracers Fast Blue, Fluoro-Gold, Mini-Ruby, Fluoro-Ruby and Fluoro-Emerald was investigated in adult rat spinal motorneurons at 1, 4, 12 and 24 weeks after tracer application to a transected muscle nerve. After 1 week, the largest number of retrogradely labeled motoneurons was found with Mini-Ruby, Fluoro-Gold and Fluoro-Ruby, while Fluoro-Emerald yielded a smaller number of labeled cells. With increasing survival time, all of these tracers exhibited a marked decrease in the number of labeled neurons. Fast Blue also produced very efficient staining after 1 week and, in addition, the number of Fast Blue-labeled cells remained constant over the entire time period studied. Also in embryonic spinal cord tissue exposed to Fast Blue. the label persisted for at least 6 months after transplantation into adult spinal cord. Double-labeling experiments combining Fast Blue with Fluoro-Gold, Mini-Ruby, Fluoro-Ruby or Fluoro-Emerald showed that all these substances were non-toxic and that the time-related decrease in the number of neurons labeled by the latter tracers was due to degradation or leakage of the dyes. Thus, Fast Blue would be the tracer of choice for motoneuronal labeling in long-term experiments, whereas the usage of the other tracers should be restricted to experiments of limited duration.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Novikova L,Novikov L,Kellerth JOdoi
10.1016/s0165-0270(97)02227-9subject
Has Abstractpub_date
1997-06-06 00:00:00pages
9-15issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(97)02227-9journal_volume
74pub_type
杂志文章abstract：:An intra-arterially perfused working heart-brainstem preparation (WH-BP) was developed to allow studies into functionally identified cardiovascular and respiratory neurones in an in vitro milieu. This report provides that first description of this preparation. Evidence is presented indicating that the WH-BP: (1) spont...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00147-6
更新日期：1996-03-01 00:00:00
abstract：BACKGROUND:Saccades are rapid eye movements used to gather information about a scene which requires both action and perception. These are usually studied separately, so that how perception influences action is not well understood. In a dual task, where the subject looks at a target and reports a decision, subtle change...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.05.010
更新日期：2014-07-30 00:00:00
abstract：BACKGROUND:Trk receptor tyrosine kinases regulate multiple important neuronal processes during the development and in the adulthood. Tyrosine phosphorylation of Trk serves as the initial step in the Trk signaling pathway and indicates receptor' autocatalytic activity. However, methods allowing simple and large-scale Tr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.11.001
更新日期：2014-01-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00144-1
更新日期：1997-12-30 00:00:00
abstract：:Experimental recordings from neurons are generally difficult to quantify. Spike patterns and membrane potential cycles are often compared qualitatively and are hence limited by human resolution and judgement. This paper describes a statistical method for quantifying individual spike patterns and membrane potential flu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90053-t
更新日期：1993-10-01 00:00:00
abstract：:The discovery of immediate early genes (IEG) has provided neuroscientists with a new functional mapping technique. Labelling of neural tissue for the protein product of IEG provides an activity map with single-cell resolution. When combined with labelling for the chemical identity of the neuron, this provides a powerf...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90023-k
更新日期：1993-04-01 00:00:00
abstract：:In this study, we developed an immuno-autoradiographic method to obtain quantitative estimates of tyrosine hydroxylase (TH) protein in tissue sections from post-mortem human brain. Protein from tissue sections containing the locus coeruleus (LC) was directly transferred to a polyvinylidene fluoride (PVDF) membrane. Im...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00211-9
更新日期：2000-06-30 00:00:00
abstract：:Analysis of the antigenic components of synaptic membranes presents several problems caused by detergents required to solubilize the proteins. These problems involve detection of the reaction between antibodies and proteins in the detergent extract as well as characterization of the individual antigens after further p...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90058-8
更新日期：1980-04-01 00:00:00
abstract：:It is often helpful to display analogue signals such as electromyograms, electroencephalograms, nerve action potentials, etc., in a regular vertical array. A special case of this display is the widely-used dot raster. This paper describes a simple circuit which enables up to 31 analogue signals to be thus displayed on...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90009-9
更新日期：1986-03-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00412-5
更新日期：2001-09-30 00:00:00
abstract：:A novel version of the 8-arm radial maze task was developed to quantify spatial learning and memory in rats subjected to transient cerebral ischemia (TCI) using the 4-VO model. This maze uses the rat's natural behavior of avoiding open, illuminated areas, and preference for a darkened, enclosed shelter. Ischemic rats ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.08.012
更新日期：2004-01-15 00:00:00
abstract：:Diffusion Magnetic Resonance Imaging (dMRI) is one of the most important contemporary non-invasive modalities for probing tissue structure at the microscopic scale. The majority of dMRI techniques employ standard single diffusion encoding (SDE) measurements, covering different sequence parameter ranges depending on th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108989
更新日期：2021-01-15 00:00:00
abstract：:Recombinant proteins are routinely expressed in heterologous expression systems such as human embryonic kidney 293 (HEK 293) cells. The efficiency of the expression is critical when the expressed protein must be characterized at the single-cell level. Here we describe a simple method by which the protein expression ef...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.09.009
更新日期：2005-03-15 00:00:00
abstract：:Transcranial Electrical Stimulation (tES) encompasses all methods of non-invasive current application to the brain used in research and clinical practice. We present the first comprehensive and technical review, explaining the evolution of tES in both terminology and dosage over the past 100 years of research to prese...
journal_title：Journal of neuroscience methods
pub_type： 历史文章,杂志文章,评审
doi：10.1016/j.jneumeth.2013.07.016
更新日期：2013-10-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.11.016
更新日期：2014-01-30 00:00:00
abstract：BACKGROUND:The application of single-cell RNA sequencing (scRNASeq) represents a unique approach to identify hundreds to millions of cells in mammalian cortical multilayers at different stages of embryogenesis. ScRNASeq technology applied to neurological studies requires the use of fresh starting materials because stan...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108960
更新日期：2021-01-01 00:00:00
abstract：:We demonstrate that high power light-emitting diodes (LED's) exhibit low-frequency noise characteristics that are clearly superior to those of quartz tungsten halogen lamps, the non-coherent light source most commonly employed when freedom from intensity variation is critical. Their extreme stability over tens of seco...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.06.009
更新日期：2005-01-30 00:00:00
abstract：:The event-related potential (ERP) and event-related field (ERF) techniques provide valuable insights into the time course of processes in the brain. Because neural signals are typically weak, researchers commonly filter the data to increase the signal-to-noise ratio. However, filtering may distort the data, leading to...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.06.011
更新日期：2012-07-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.08.010
更新日期：2005-03-30 00:00:00
abstract：:Foraging is a rapid, side-to-side movement of the nose generated by Caenorhabditis elegans as it explores its environment. In this paper, we present an automated method to detect and analyze foraging behavior of C. elegans in a video sequence. Several morphological image-processing methods are used to locate the preci...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.027
更新日期：2008-06-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.10.012
更新日期：2019-01-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.01.020
更新日期：2009-04-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.04.006
更新日期：2016-07-15 00:00:00
abstract：BACKGROUND:Providing weight support facilitates locomotion in spinal cord injured animals. To control weight support, robotic systems have been developed for treadmill stepping and more recently for overground walking. NEW METHOD:We developed a novel device, the body weight supported ambulatory rodent trainer (i.e. BA...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.03.015
更新日期：2015-05-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.11.007
更新日期：2018-01-15 00:00:00
abstract：BACKGROUND:Automated segmentation of brain structures is an important task in structural and functional image analysis. We developed a fast and accurate method for the striatum segmentation using deep convolutional neural networks (CNN). NEW METHOD:T1 magnetic resonance (MR) images were used for our CNN-based segmenta...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.10.007
更新日期：2016-12-01 00:00:00
abstract：:A light microscopical, non-fluorescent, retrograde double-labeling technique is described. Cholera toxin B-subunit (CTb) and a conjugate of wheatgerm agglutinin and bovine serum albumin coupled to 10 nm gold particles (gold-lectin) are both excellent retrograde tracers and, when visualized by means of immunohistochemi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00034-e
更新日期：1995-09-01 00:00:00
abstract：:The objective of visual systems neuroscience has shifted over the past few years from determining the receptive fields of cells towards the understanding of higher level cognition in awake animals viewing natural stimuli. In experiments with awake animals it is important to control the relevant aspects of behavior. Mo...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00423-x
更新日期：2001-09-30 00:00:00
abstract：:A newly designed combination microelectrode-injection system is described which can be used to record electrophysiological responses of individual neurons in the primate brain to local administration of small quantities of drugs or other compounds. The assembly of the system is simple, and the materials used are inexp...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.03.015
更新日期：2004-09-30 00:00:00
abstract：:Rapid prototyping (RP) is a useful method for designing and fabricating a wide variety of devices used for neuroscience research. The present study confirms the utility of using fused deposition modeling, a specific form of RP, to produce three devices commonly used for basic science experimentation. The accuracy and ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.03.011
更新日期：2008-07-30 00:00:00
