Abstract:
:The present study evaluated high-speed chronoamperometry as a method for measuring the clearance of serotonin (5-HT) from extracellular space in vivo. Male Sprague-Dawley rats were anaesthetized and a Nafion-coated, carbon fiber electrode, attached to a multibarrel pipette, was lowered into the subgranular layer of the dentate gyrus, a region which receives dense serotonergic innervation, or the corpus callosum, a fiber tract relatively devoid of the 5-HT transporter (SERT). Serotonin, pressure ejected into these regions, produced replicable electrochemical signals. The amplitude and time course of the signals were significantly prolonged in the corpus callosum compared to the dentate gyrus. Similarly, signals produced by locally applied 5-HT in the dentate gyrus of rats following destruction of hippocampal serotonergic innervation with 5,7-dihydroxytryptamine (5,7-DHT), were significantly enhanced compared to those observed in control animals. The time course of the 5-HT signal was significantly prolonged by local application of the selective 5-HT reuptake inhibitor, fluvoxamine, into the dentate gyrus. By contrast, fluvoxamine did not modify the clearance of 5-HT when locally applied into the dentate gyrus of 5,7-DHT lesioned rats or into the corpus callosum of intact rats. Taken together, these data demonstrate that in intact rats, the SERT contributes to the clearance of exogenously applied 5-HT from the extracellular space. Under the experimental conditions used in this study, high-speed chronoamperometry proved to be a reliable method for directly measuring extracellular 5-HT and appears to be a valuable tool for the study of 5-HT clearance by the SERT in vivo.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Daws LC,Toney GM,Davis DJ,Gerhardt GA,Frazer Adoi
10.1016/s0165-0270(97)00144-1subject
Has Abstractpub_date
1997-12-30 00:00:00pages
139-50issue
1-2eissn
0165-0270issn
1872-678Xpii
S0165-0270(97)00144-1journal_volume
78pub_type
杂志文章abstract::Cobalamin (Cbl) utilization as a cofactor for methionine synthase and methylmalonyl-CoA mutase is dependent on the transport of Cbl through lysosomes and its subsequent delivery to the cytosol and mitochondria. We speculated that neuropathological conditions that impair lysosomal function (e.g., age-related lipofuscin...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.04.008
更新日期:2013-07-15 00:00:00
abstract::Two-photon laser scan microscopy carries many advantages for work on brain slices and bulk tissue. However, it has very low signal levels compared to conventional fluorescence microscopy. This is disadvantageous in fast imaging applications when photon shot noise is limiting. Working on brain slices with excitation po...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(99)00103-x
更新日期:1999-10-15 00:00:00
abstract::Computer-based sleep scoring systems are often calibrated by reference to a conventional visual analysis of electroencephalographic (EEG) and electromyographic (EMG) traces. However, these types of data place high demands on digital storage capacity which may limit the duration or feasibility of some studies. The pres...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.04.004
更新日期:2005-10-15 00:00:00
abstract::An important issue for neurophysiological studies of the visual system is the definition of the region of the visual field that can modify a neuron's activity (i.e., the neuron's receptive field - RF). Usually a trade-off exists between precision and the time required to map a RF. Manual methods (qualitative) are fast...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.09.012
更新日期:2014-01-15 00:00:00
abstract::The goal of the current study is to find a suitable classifier for electroencephalogram (EEG) data derived from a new learning paradigm which aims at communication in paralysis. A reflexive semantic classical (Pavlovian) conditioning paradigm is explored as an alternative to the operant learning paradigms, currently u...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.09.013
更新日期:2012-01-15 00:00:00
abstract::Electrical stimulation of the rat incisor pulp has been frequently used as a method to study the mechanisms of pain. However, several of the techniques used to stimulate nociceptive nerves in these teeth also tend to excite non-nociceptive nerves in adjacent tissues. This report deals with the development and verifica...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(87)90092-6
更新日期:1987-11-01 00:00:00
abstract:BACKGROUND:MRI-histology correlation studies of the ex vivo brain mostly employ fresh, extracted (ex situ) specimens, aldehyde fixed by immersion, which has several disadvantages for MRI scanning (e.g. deformation of the organ). A minority of studies are done ex vivo-in situ (unfixed brain), requiring an MRI scanner re...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108903
更新日期:2020-11-01 00:00:00
abstract::Three-dimensional (3D) reconstruction from serial sections that are undistorted and true-to-life is only possible when a reference system exists that allows correct matching of the section drawings. This paper describes a simple method for producing reference lines that ensures proper alignment of the drawings. (1) Th...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90162-8
更新日期:1988-12-01 00:00:00
abstract:BACKGROUND:Cytokines are key signaling molecules within the immune system that regulate a host's response to pathogens and neuronal damage. Aberrant cytokine signaling has been implicated in many neurological diseases. Therefore, accurately measuring cytokine concentrations within the brain is crucial. NEW METHOD:This...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108601
更新日期:2020-01-22 00:00:00
abstract::The design of a simple and inexpensive battery powered lamp controller for microscope lamps commonly used in patch clamp and other biophysical studies is described. The circuit linearizes the normally exponential voltage-intensity curve of standard 12-V lamps by using an operational amplifier to provide feedback to th...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(91)90098-k
更新日期:1991-10-01 00:00:00
abstract:BACKGROUND:Normal defaecation involves activation of anorectal mechanoreceptors responsive to pressure and stretch. The aim of this study was to develop selective anal and rectal mucosal light-touch stimulation suitable for measurement of cortical evoked potentials (EPs) in order to explore the sensory arm of these pat...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.09.006
更新日期:2015-12-30 00:00:00
abstract:BACKGROUND:Large animal models of human neurological disorders are advantageous compared to rodent models due to their neuroanatomical complexity, longevity and their ability to be maintained in naturalised environments. Some large animal models spontaneously develop behaviours that closely resemble the symptoms of neu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.01.018
更新日期:2017-12-01 00:00:00
abstract::Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.09.034
更新日期:2009-02-15 00:00:00
abstract::Recently, resting state functional connectivity (RSFC) studies based on fMRI and EEG/MEG have provided valuable insight into the intrinsic functional architecture of the human brain. However, whether functional near infrared spectroscopy (fNIRS), a suitable imaging method for infant and patient populations, can be use...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.11.010
更新日期:2010-02-15 00:00:00
abstract:BACKGROUND:Accurate and reliable measurement of leukoaraiosis, or MR-detected white, matter hyper-intensity (WMH) burden in subjects with acute ischemic stroke (AIS) is important for, ongoing research studies and future models of risk and outcome prediction, but the presence of a, cerebral infarct may complicate measur...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.10.009
更新日期:2014-01-15 00:00:00
abstract::Brain dialysis has become an important tool for investigating changes in the extracellular levels of neurotransmitters. This paper reviews the experimental variables and criteria that should be considered when interpreting data obtained with the brain dialysis method. Brain dialysis can provide important direct inform...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/0165-0270(90)90039-i
更新日期:1990-09-01 00:00:00
abstract::The mouse is an extremely valuable model for studying vagal development in relation to strain differences, genetic variation, gene manipulations or pharmacological manipulations. Therefore, a method using 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was developed for labeling vagal innervat...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.03.001
更新日期:2007-07-30 00:00:00
abstract::Intrinsic signal imaging (ISI) measures changes in light reflectance from the illuminated cortex (intrinsic signals or IS) attributed to various vascular and metabolic sources that, when using illumination in the 600 nm range, appear to co-localize with neuronal activity. Given the multiple sources contributing to the...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00180-1
更新日期:2000-04-15 00:00:00
abstract:BACKGROUND:Over the past decade, pattern decoding techniques have granted neuroscientists improved anatomical specificity in mapping neural representations associated with function and cognition. Dynamical patterns are of particular interest, as evidenced by the proliferation and success of frequency domain methods tha...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.06.019
更新日期:2018-10-01 00:00:00
abstract::A method is described for the production of an electrode capable of monitoring and modulating the input-output relationship of thalamic neurons. Tungsten-in-glass electrodes were manufactured with the ability to simultaneously record lateral geniculate nucleus action potentials and associated, retinally evoked S-poten...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90126-c
更新日期:1993-09-01 00:00:00
abstract::Parkinson's disease (PD) exhibits symptoms of motor dysfunction such as tremor, akinesia and rigidity. Agents that selectively disrupt or destroy catecholaminergic systems, such as reserpine, methamphetamine, 6-hydroxydopamine and 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine, have been used to develop PD models and to...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.12.019
更新日期:2009-04-15 00:00:00
abstract::We propose a flexible hierarchical Bayesian nonparametric modeling approach to compare the spiking patterns of neurons recorded under multiple experimental conditions. In particular, we showcase the application of our statistical methodology using neurons recorded from the supplementary eye field region of the brains ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.09.017
更新日期:2012-01-15 00:00:00
abstract::The relationship between the levels of cytochrome oxidase and gamma-aminobutyric acid (GABA) was investigated within single neurons by double labeling the 2 markers in the same section. Double staining was equally effective when immunogold-silver staining of GABA was followed by indirect immunoperoxidase labeling of c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90129-5
更新日期:1989-12-01 00:00:00
abstract:BACKGROUND:Certain inherited progressive neurodegenerative disorders, such as spinocerebellar ataxia (SCA), affect neurons in large areas of the central nervous system (CNS). The selective expression of disease-causing and therapeutic genes in susceptible regions and cell types is critical for the generation of animal ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.12.004
更新日期:2014-02-15 00:00:00
abstract::We present a client-server application for the distributed multivariate analysis of time series using standard PCs. We here concentrate on analyses of multichannel EEG/MEG data, but our method can easily be adapted to other time series. Due to the rapid development of new analysis techniques, the focus in the design o...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.09.002
更新日期:2006-04-15 00:00:00
abstract:BACKGROUND:The light-induced release of neurotransmitters from caging chromophores provides a powerful means to study the underlying receptors in a physiologically relevant context. Surprisingly, most caged neurotransmitters, including the widely used 4-methoxy-7-nitroindolinyl (MNI)-glutamate, show strong antagonism a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.10.013
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Multiscale entropy (MSE) estimates the predictability of a signal over multiple temporal scales. It has been recently applied to study brain signal variability, notably during aging. The grounds of its application and interpretation remain unclear and subject to debate. METHOD:We used both simulated and exp...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.09.004
更新日期:2016-11-01 00:00:00
abstract::We have developed a new method for culturing cells that maintains their health and sterility for many months. Using conventional techniques, primary neuron cultures seldom survive more than 2 months. Increases in the osmotic strength of media due to evaporation are a large and underappreciated contributor to the gradu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00412-5
更新日期:2001-09-30 00:00:00
abstract:BACKGROUND:Evaluation of motor deficits in rodents is mostly restricted to limb motor tests that are often high stressors for the animals. NEW METHOD:To test rodents for orofacial motor impairments in a stress-free environment, we established the pasta gnawing test by measuring the biting noise of mice that eat a piec...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.10.006
更新日期:2016-12-01 00:00:00
abstract::Exceptionally clear Golgi-Nissl sections of 300 micron thickness have been morphometrically studied by light microscopy using oil immersion objectives. The clarity results from a new variation of a staining procedure that combines Golgi and Nissl images in one section. A viewing technique has been developed that permi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(81)90045-5
更新日期:1981-08-01 00:00:00