Abstract：
:The discovery of immediate early genes (IEG) has provided neuroscientists with a new functional mapping technique. Labelling of neural tissue for the protein product of IEG provides an activity map with single-cell resolution. When combined with labelling for the chemical identity of the neuron, this provides a powerful tool for the investigation of specific cell populations along a neuraxis. Here we describe in detail a method which allows simultaneous bright-field visualization of neurochemically identified cells displaying increased IEG expression. This technique is evaluated in tissue from rats subjected to stimuli known to induce the expression of the IEG c-fos in various medullary catecholaminergic and hypothalamic neurosecretory cell groups. A 2-colour immunoperoxidase technique was used to visualize Fos, the nuclear protein product of c-fos, and the cytoplasmic antigens tyrosine hydroxylase (TH), phenylethanolamine N-methyl transferase (PNMT), oxytocin (OT) and vasopressin (VP). This involved simultaneous application of primary antibodies raised in different species followed by sequential application of appropriate biotinylated secondary antibodies and the avidin-biotin-peroxidase technique. Fos was visualized with nickel-intensified diaminobenzidine (Ni-DAB) in the first sequence while TH, PNMT, OT or VP were visualized with DAB alone, resulting in readily distinguishable black and amber reaction products, respectively. This dual immunoperoxidase technique is time saving compared to techniques using sequential application of primary antibodies and avoids the disadvantages associated with fluorescence techniques.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Smith DW,Day TAdoi
10.1016/0165-0270(93)90023-ksubject
Has Abstractpub_date
1993-04-01 00:00:00pages
73-83issue
1-2eissn
0165-0270issn
1872-678Xpii
0165-0270(93)90023-Kjournal_volume
47pub_type
杂志文章abstract：:Threshold depolarization is defined as the amplitude of the depolarization that will just bring a neurone to its firing threshold. Here, a novel technique is described for the measurement of threshold depolarization in resting human motoneurones. The tibial nerve was stimulated at an intensity that was just sufficient...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.09.010
更新日期：2004-02-15 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.12.003
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2014.02.004
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.11.010
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90098-k
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2007.01.008
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2015.09.006
更新日期：2015-12-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.07.005
更新日期：2007-10-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
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更新日期：2019-09-01 00:00:00
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2010-01-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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