Abstract：
:An important factor in reducing variability in mouse test outcomes has been to develop assays that can be used for continuous automated home cage assessment. Our experience has shown that this has been most evidenced in long-term assessment of wheel-running activity in mice. Historically, wheel-running in mice and other rodents have been used as a robust assay to determine, with precision, the inherent period of circadian rhythms in mice. Furthermore, this assay has been instrumental in dissecting the molecular genetic basis of mammalian circadian rhythms. In teasing out the elements of this test that have determined its robustness - automated assessment of an unforced behaviour in the home cage over long time intervals - we and others have been investigating whether similar test apparatus could be used to accurately discriminate differences in distinct behavioural parameters in mice. Firstly, using these systems, we explored behaviours in a number of mouse inbred strains to determine whether we could extract biologically meaningful differences. Secondly, we tested a number of relevant mutant lines to determine how discriminative these parameters were. Our findings show that, when compared to conventional out-of-cage phenotyping, a far deeper understanding of mouse mutant phenotype can be established by monitoring behaviour in the home cage over one or more light:dark cycles.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Bains RS,Wells S,Sillito RR,Armstrong JD,Cater HL,Banks G,Nolan PMdoi
10.1016/j.jneumeth.2017.04.014subject
Has Abstractpub_date
2018-04-15 00:00:00pages
37-47eissn
0165-0270issn
1872-678Xpii
S0165-0270(17)30113-9journal_volume
300pub_type
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