Abstract:
:We investigated the usefulness of the Overall Rotarod Performance (ORP) test for evaluating overall locomotory ability in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-injected-mouse model of Parkinson's disease (PD). For this procedure, the mice are pretrained on the rotarod and then tested at a series of increasing speeds, recording the time that the animal remains on the rod at each speed; the overall rod performance (ORP) of each animal is then calculated as the area under the curve in a plot of time-on-the-rod against rotation speed. At 15-day intervals, C57BL/6 mice were injected (or sham-injected) with MPTP, with ORP testing 7-10 days after each injection. After the fourth injection (day 45), mice in the treated group showed clearly lower ORP than mice in the control group (70-90% reduction in ORP), and were thus considered effectively lesioned. Subsequently, we investigated the short-term effects of apomorphine and L-DOPA on ORP in MPTP-treated mice. Apomorphine (at 0.5 or 2.5 mg/kg) had no significant effect, while L-DOPA (at 80 but not at 40 mg/kg) caused almost complete short-term recovery of pretreatment ORP. By about 100 days after the last MPTP injection, MPTP-treated mice showed partial long-term recovery of ORP; at this stage the mice were killed for tyrosine hydroxylase (TH) immunohistochemistry studies. TH immunoreactivity in the striatum showed a strong positive correlation with ORP as tested on day 100. We conclude that the ORP test is useful for evaluating motor deficit in MPTP-treated mice, and the effects of subsequent treatments.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Rozas G,López-Martín E,Guerra MJ,Labandeira-García JLdoi
10.1016/s0165-0270(98)00078-8subject
Has Abstractpub_date
1998-09-01 00:00:00pages
165-75issue
2eissn
0165-0270issn
1872-678Xpii
S0165027098000788journal_volume
83pub_type
杂志文章abstract::Diffusion Magnetic Resonance Imaging (dMRI) is one of the most important contemporary non-invasive modalities for probing tissue structure at the microscopic scale. The majority of dMRI techniques employ standard single diffusion encoding (SDE) measurements, covering different sequence parameter ranges depending on th...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108989
更新日期:2021-01-15 00:00:00
abstract::Morphometric estimation of neuronal processes is currently laborious and time-consuming, since the individual processes (axons and dendrites) have to be traced manually. In order to facilitate the measurement of cellular processes, we have tested a series of parameters derived from the contour and the convex hull of a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)00165-9
更新日期:1998-01-31 00:00:00
abstract::Functionally relevant large scale brain dynamics operates within the framework imposed by anatomical connectivity and time delays due to finite transmission speeds. To gain insight on the reliability and comparability of large scale brain network simulations, we investigate the effects of variations in the anatomical ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.07.007
更新日期:2009-09-30 00:00:00
abstract:BACKGROUND:The input signals of electroencephalography (EEG) based brain computer interfaces (BCI) are extensively acquired from scalp with a multi-channel system. However, multi-channel signals might contain redundant information and increase computational complexity. Furthermore, using only effective channels, rather...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.12.004
更新日期:2019-02-01 00:00:00
abstract::We describe a family of 6 computer programs that measure, analyse and create graphic displays of complex motor patterns. The programs create lists of times at which successive bursts of impulses in different nerves started and stopped, and use these lists to calculate the periods and durations of these bursts and to c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(87)90020-3
更新日期:1987-01-01 00:00:00
abstract::The Porsolt forced swim test (FST) is one of the most widely used behavioral tests in the evaluation of the antidepressant effects of drugs. It is based on the fact that these drugs reduce the depression-related behaviors of learned helplessness. The model has been modified for use in mice. In contrast to rats, mice a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.04.010
更新日期:2005-11-30 00:00:00
abstract::A novel test for the selective identification of anxiolytic and anxiogenic drug effects in the rat is described, using an elevated + -maze consisting of two open arms and two enclosed arms. The use of this test for detecting such drug effects was validated behaviourally, physiologically, and pharmacologically. Rats ma...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(85)90031-7
更新日期:1985-08-01 00:00:00
abstract::The detection of extracellular potentials by means of multi-electrode arrays (MEA) is a useful technique for multi-site long-term monitoring of cultured neuronal activity with single-cell resolution. To optimize the geometry of the MEA it is advantageous to localize the cellular compartments that constitute the genera...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(02)00043-2
更新日期:2002-05-30 00:00:00
abstract::A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90136-f
更新日期:1993-01-01 00:00:00
abstract:BACKGROUND:Multiscale entropy (MSE) estimates the predictability of a signal over multiple temporal scales. It has been recently applied to study brain signal variability, notably during aging. The grounds of its application and interpretation remain unclear and subject to debate. METHOD:We used both simulated and exp...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.09.004
更新日期:2016-11-01 00:00:00
abstract:BACKGROUND:Functional Magnetic Resonance Imaging (fMRI) is used for exploring brain functionality, and recently it was applied for mapping the brain connection patterns. To give a meaningful neurobiological interpretation to the connectivity network, it is fundamental to properly define the network framework. In partic...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.03.004
更新日期:2014-05-15 00:00:00
abstract:BACKGROUND:Controlled cortical impact (CCI) has emerged as one of the most flexible and clinically applicable approaches for the induction of traumatic brain injury (TBI) in rodents and other species. Although this approach has been shown to model cognitive and functional outcomes associated with TBI in humans, recent ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.10.027
更新日期:2019-01-15 00:00:00
abstract::Intrathecal (IT) administration is an important route of drug delivery, and its modelling in a large animal species is of critical value. Although domestic swine is the preferred species for preclinical pharmacology, no minimally invasive method has been established to deliver agents into the IT space. While a "blind"...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.03.006
更新日期:2013-05-30 00:00:00
abstract::Event related potentials (ERPs) are very feeble alterations in the ongoing electroencephalogram (EEG) and their detection is a challenging problem. Based on the unique time-based parameters derived from wavelet coefficients and the asymmetry property of wavelets a novel algorithm to separate ERP components in single-t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.06.004
更新日期:2014-08-15 00:00:00
abstract::We used 24 sacral dorsal roots of the rat to analyze amplitude and area changes of biphasic and monophasic compound action potentials (CAPs) at 4 conduction distances. Both the CAPs of the A-fiber and C-fiber were analyzed. The changes were examined with the paired t test and linear regression. All the variables decre...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(93)90064-x
更新日期:1993-03-01 00:00:00
abstract:BACKGROUND:Flow cytometry is an efficient and powerful technique to characterize and quantify numerous cells. However, the strengths of this technique have not been widely harnessed in neurosciences due to the critical step of CNS tissue preparation into a single cell suspension. Previous reports assessed either neural...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.03.021
更新日期:2015-05-30 00:00:00
abstract::Voltage-sensitive dyes (VSDs) and optical imaging are useful for studying spatiotemporal patterns of population neuronal activity in cortical tissue. Using a photodiode array and absorption dyes we were able to detect neuronal activity in single trials before it could be detected by local field potential (LFP) recordi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00511-8
更新日期:2002-03-30 00:00:00
abstract::This research evaluated changes in surface electromyographic (SEMG) spike shape across different levels of isometric force. Ninety-six subjects generated three 5-s isometric step contractions of the elbow flexors at 40, 60, 80, and 100% of maximal voluntary contraction (MVC). Force and bipolar SEMG activity were monit...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.07.004
更新日期:2007-01-15 00:00:00
abstract::After a review of the ocular artifact reduction literature, a high-throughput method designed to reduce the ocular artifacts in multichannel continuous EEG recordings acquired at clinical EEG laboratories worldwide is proposed. The proposed method belongs to the category of component-based methods, and does not rely o...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.01.007
更新日期:2011-03-15 00:00:00
abstract:BACKGROUND:To map and quantify the proton exchange rate (kex) of brain tissues using improved omega plots in ischemic stroke patients and to investigate whether kex can serve as a potential endogenous surrogate imaging biomarker for detecting the metabolic state and the pathologic changes due to ischemic stroke. NEW M...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108926
更新日期:2020-12-01 00:00:00
abstract::The human movement analysis panel (HMAP) measures separable components of arm motion and simple and complex finger coordination. HMAP testing takes 30min to administer. In separate experiments we have validated the HMAP against the standard grooved pegboard and measures of gait speed, and demonstrated important learni...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.06.015
更新日期:2007-09-30 00:00:00
abstract:BACKGROUND:There is a growing need for analyzing medical data such as brain connectomes. However, the unavailability of large-scale training samples increases risks of model over-fitting. Recently, deep learning (DL) architectures quickly gained momentum in synthesizing medical data. However, such frameworks are primar...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2020.108988
更新日期:2020-11-04 00:00:00
abstract:BACKGROUND:Magnetoencephalography (MEG) and Electroencephalography (EEG) are noninvasive techniques to study the electrophysiological activity of the human brain. Thus, they are well suited for real-time monitoring and analysis of neuronal activity. Real-time MEG/EEG data processing allows adjustment of the stimuli to ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.03.020
更新日期:2018-06-01 00:00:00
abstract:BACKGROUND:The introduction of viral transneuronal tracers in the toolbox of neural tract-tracing methods has been an important addition in the field of connectomics for deciphering circuit-level architecture of the nervous system. One of the added values of viral compared to conventional retrograde tracers, in particu...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2020.108872
更新日期:2020-10-01 00:00:00
abstract::The analgesic effects of various agents were evaluated in knee joint monoarthritic rats where arthritis was induced by monosodium urate (MSU) crystals injected into the knee joint cavities. The weight supported by each hind limb was measured quantitatively using pressure transducers, which revealed dose-dependent reco...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(90)90026-c
更新日期:1990-08-01 00:00:00
abstract:BACKGROUND:To establish a novel rat model of middle cerebral artery occlusion (MCAO) complicated with prior venous stagnation, and to investigate the role of cerebral venous drainage in neural injury after acute cerebral infarction. NEW METHOD:Eighteen SD rats were randomly divided into two groups: control group and j...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.01.016
更新日期:2019-04-15 00:00:00
abstract:BACKGROUND:Extracellular recordings are performed by inserting electrodes in the brain, relaying the signals to external power-demanding devices, where spikes are detected and sorted in order to identify the firing activity of different putative neurons. A main caveat of these recordings is the necessity of wires passi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.04.018
更新日期:2014-06-15 00:00:00
abstract:UNLABELLED:Lobeline is a potential smoking cessation drug with affinity for the α4β2 nicotinic acetylcholine receptor and may inhibit the blood-brain barrier (BBB) amine transporter. The goal of this work was to use PET imaging to evaluate the effects of lobeline on the kinetic properties of [(18)F]nifene in the rat br...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.01.018
更新日期:2013-04-15 00:00:00
abstract::A protocol has been established for the staining of myelin in frozen sections. While the new method is relatively fast and simple, it eliminates the problems routinely encountered with myelin stains such as blotchiness and uneven staining. Modifications were introduced into the procedures in order to obtain excellent ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(83)90012-2
更新日期:1983-03-01 00:00:00
abstract::The force-plate actometer is a relatively new computer-based instrument with high temporal and spatial resolution that has been used to measure the behavioral effects of genetic restriction (e.g., inbred mice) and drugs (e.g., dopaminergic agonists and antagonists) on a variety of behaviors in rodents, including locom...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.10.002
更新日期:2006-05-15 00:00:00