Abstract：
:A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in the tip prevents bulk mixing of the 2 phases within the pipette. This configuration makes it possible to remove Cl- from the membrane surface but maintain it at the electrode surface. The presence of the Cl(-)-free phase in the bottom of the patch pipette does not reduce the electrical stability of the recording electrode.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Kleene SJdoi
10.1016/0165-0270(93)90136-fsubject
Has Abstractpub_date
1993-01-01 00:00:00pages
11-6issue
1eissn
0165-0270issn
1872-678Xpii
0165-0270(93)90136-Fjournal_volume
46pub_type
杂志文章abstract：:Isolated turtle brain/eye preparation has recently been used as a bloodless animal model for detecting the magnetic resonance imaging (MRI) signal changes produced by visually evoked neuronal currents. The present work aims to determine whether checkerboard-patterned or full field flash (blank) stimulation should be u...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.12.005
更新日期：2010-03-15 00:00:00
abstract：:The amplitude of the maximal direct motor response (Mmax) elicited by supramaximal peripheral nerve stimulation can vary with time and with changes in muscle length. We sought to investigate the variability in the amplitude of Mmax in the human wrist flexors (flexor carpi radialis, FCR) at a constant joint position du...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.03.016
更新日期：2005-12-15 00:00:00
abstract：BACKGROUND:The widespread use of data-driven methods, such as independent component analysis (ICA), for the analysis of functional magnetic resonance imaging data (fMRI) has enabled deeper understanding of neural function. However, most popular ICA algorithms for fMRI analysis make several simplifying assumptions, thus...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.03.012
更新日期：2016-05-01 00:00:00
abstract：BACKGROUND:There is a growing need for analyzing medical data such as brain connectomes. However, the unavailability of large-scale training samples increases risks of model over-fitting. Recently, deep learning (DL) architectures quickly gained momentum in synthesizing medical data. However, such frameworks are primar...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108988
更新日期：2020-11-04 00:00:00
abstract：:A computational model has been developed for the action potential and, more generally, the electrical behaviour of the rat sympathetic neurone. The neurone is simulated as a complex system in which five voltage-dependent conductances (gNa, gCa, gKV, gA, gKCa), one Ca2+-dependent voltage-independent conductance (gAHP) ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00199-4
更新日期：1998-04-30 00:00:00
abstract：:Epoxy-insulated tungsten microelectrodes can be used once or twice in our lab before the impedance becomes too low. Dipping the electrodes in epoxy followed by curing restores their initial high impedance which is associated with good isolation of single neurons. It is a cost effective and simple procedure. ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00365-5
更新日期：2003-03-15 00:00:00
abstract：:Scanning ion conductance microscopy (SICM) takes advantage of the increase in the resistance which occurs if a glass microelectrode is closely approached to a poorly conducting membrane (Science 243 (1989) 641) and has been shown to be a promising technique to study membranes of living cells (Biophys J 73 (1997a) 653;...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00023-7
更新日期：2002-05-15 00:00:00
abstract：:Imaging technologies, such as intrinsic optical imaging (IOI), functional magnetic resonance imaging (fMRI) or multiphoton microscopy provide excellent opportunities to study the relationship between functional signals recorded from a cortical area and the underlying anatomical structure. This, in turn, requires accur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.07.029
更新日期：2011-09-30 00:00:00
abstract：:A technique for the preparation and recording of mammalian supraoptic nucleus neurosecretory neurons maintained in vitro through perfusion of the anterior cerebral artery is described. A slice of basal diencephalon measuring approximately 8 X 8 X 2 mm that includes the pituitary stalk and medial preoptic-anterior peri...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90002-x
更新日期：1983-03-01 00:00:00
abstract：BACKGROUND:Statistical models that predict neuron spike occurrence from the earlier spiking activity of the whole recorded network are promising tools to reconstruct functional connectivity graphs. Some of the previously used methods are in the general statistical framework of the multivariate Hawkes processes. However...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.12.026
更新日期：2018-03-01 00:00:00
abstract：BACKGROUND:Neurotrauma patients face major neurological sequelae. The failure in the preclinical-to-clinical translation of candidate therapies could be due to poor evaluation of rodent behaviours after neurotrauma. NEW METHOD:A home cage automated system was used to study the long term behaviour of individual rats wi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.04.005
更新日期：2019-06-01 00:00:00
abstract：:Biolistics, also known as particle-mediated gene transfer, has been used as an effective, method to transfect primary neurons in cultured slices when all other methods have proven unsuccessful. Most of these uses have provided qualitative or semi-quantitative data based on visual assays such as immunohistochemistry. I...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00117-4
更新日期：1998-10-01 00:00:00
abstract：BACKGROUND:Application of functional Near InfraRed Spectroscopy (fNIRS) in neurology is still limited as a good optical coupling and optimized optode coverage of specific brain regions remains challenging, notably for prolonged monitoring. METHODS:We propose to evaluate a new procedure allowing accurate investigation ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.08.006
更新日期：2018-11-01 00:00:00
abstract：:We present an effective technique for culture and expansion of Schwann cells (SC) from adult peripheral nerves. Cultures from adult mouse sciatic nerves (one to six nerves per culture) in defined medium showed markedly higher purity and density of SC when the nerve was predegenerated in vivo for 7 days than when it wa...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00221-1
更新日期：2000-06-30 00:00:00
abstract：:Single, i.e. unconnected spinal or cerebral neurons from chick embryos rapidly regenerate morphologically identifiable axons and dendrites when cultured in a three-dimensional (3-D) extracellular matrix (ECM) consisting of a hydrated native collagen lattice. We now show that it is possible to study the intrinsic elect...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90052-5
更新日期：1987-07-01 00:00:00
abstract：:Genetic intervention is a powerful means for answering basic biological questions about molecular events taking place in brain cells underlying complex behaviors. Traditional transfection method has proved difficult to apply to neuronal cells. We therefore described an alternative procedure for introducing DNA into cu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)02259-0
更新日期：1997-07-18 00:00:00
abstract：:An isoelectric focusing (IEF) technique using direct peroxidase or peroxidase-antiperoxidase (PAP) overlay staining for detection of cerebrospinal fluid (CSF) oligoclonal IgG is described. The method involves chemical fixation of all proteins in the gel followed by application of peroxidase coupled antiserum as a spec...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90035-7
更新日期：1980-10-01 00:00:00
abstract：:Alzheimer's disease (AD) is a progressive degenerative disorder of the brain characterized by a slow, progressive decline in cognitive function and behavior. As the disease advances, persons have a tough time with daily tasks like using the phone, cooking, handling money or driving the car. AD affects 15 million peopl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.10.036
更新日期：2009-03-15 00:00:00
abstract：BACKGROUND:Magnetoencephalography (MEG) and Electroencephalography (EEG) are noninvasive techniques to study the electrophysiological activity of the human brain. Thus, they are well suited for real-time monitoring and analysis of neuronal activity. Real-time MEG/EEG data processing allows adjustment of the stimuli to ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.03.020
更新日期：2018-06-01 00:00:00
abstract：:The technique named detrended fluctuation analysis (DFA) has been used to reveal the presence of long-range temporal correlations (LRTC) and scaling behavior (SB) in electroencephalographic (EEG) recordings. The occurrence of these phenomena seems to be a salient characteristic of the healthy human brain and alteratio...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.01.010
更新日期：2008-05-30 00:00:00
abstract：:Electrical stimulation of the rat incisor pulp has been frequently used as a method to study the mechanisms of pain. However, several of the techniques used to stimulate nociceptive nerves in these teeth also tend to excite non-nociceptive nerves in adjacent tissues. This report deals with the development and verifica...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90092-6
更新日期：1987-11-01 00:00:00
abstract：:Since pain is an important symptom in arthritis, useful behavioral indices for pain in experimental arthritis animal models are important tools for investigative work on arthritis. The purpose of this study was to develop simple and quantifiable behavioral tests, which would represent the level of arthritic pain that ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00011-0
更新日期：2002-03-30 00:00:00
abstract：:Testing electrophysiological recording equipments is an important task in multi-channel extracellular in vivo electrophysiology. In this paper, a miniature, battery powered multi-channel electrophysiological signal-generator (ESG) is described that was designed for this purpose. The device is based on a Xilinx CPLD (C...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.05.017
更新日期：2007-09-15 00:00:00
abstract：:Cerebrocortical neurons that store and release zinc synaptically are widely recognized as critical in maintenance of cortical excitability and in certain forms of brain injury and disease. Through the last 20 years, this synaptic release has been observed directly or indirectly and reported in more than a score of pub...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.11.014
更新日期：2006-06-30 00:00:00
abstract：BACKGROUND:Peripheral autonomic nerves control visceral organs and convey information regarding their functional states and are, therefore, potential targets for new therapeutic and diagnostic approaches. Conventionally recorded multi-unit nerve activity in vivo undergoes slow differential drift of signal and noise amp...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2020.108683
更新日期：2020-05-15 00:00:00
abstract：BACKGROUND:The local field potential (LFP) is usually calculated from current sources arising from transmembrane currents, in particular in asymmetric cellular morphologies such as pyramidal neurons. NEW METHOD:Here, we adopt a different point of view and relate the spiking of neurons to the LFP through efferent synap...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108871
更新日期：2020-10-01 00:00:00
abstract：OBJECTIVES:Schizophrenia is a predominant product of pathological alterations distributed throughout interconnected neural systems. Designing new objectively diagnostic methods are burning questions. Dynamical functional connectivity (DFCs) methodology based on fMRI data is an effective lever to investigate changeabili...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.10.011
更新日期：2019-01-01 00:00:00
abstract：BACKGROUND:Deep brain stimulation (DBS) has treatment efficacy in neurological and psychiatric disorders such as Parkinson's disease and major depression. Electroencephalography (EEG) is a versatile neurophysiological tool that can be used to better understand DBS treatment mechanisms. DBS causes artifacts in EEG recor...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.09.002
更新日期：2014-11-30 00:00:00
abstract：:Preferential exploration of novel locations and objects by rodents has been used to test the effects of various manipulations on object recognition memory. However, manual scoring is time-consuming, requires extensive training, and is subject to inter-observer variability. Since rodents explore primarily by sniffing, ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.11.002
更新日期：2008-03-15 00:00:00
abstract：:Some basic methodological issues concerning the use of the fluorescent tracers Fast blue (FB) and Diamidino yellow (DY) were studied using the projections of the red nucleus to the nucleus interpositus anterior (NIA) of the cerebellum of the cat. On standard Nissl-stained sections, it was possible to delineate 4 disti...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90100-2
更新日期：1987-09-01 00:00:00
