Abstract：
BACKGROUND:The detection of high-frequency oscillations (HFOs, ripples: 80-200 Hz, fast ripples: 250-500 Hz) is often based on considering ripples or fast ripples in isolation; overlapping ripples and fast ripples are excluded from further analysis. Here, we studied overlapping HFOs during spontaneous seizures in pilocarpine-treated animals. NEW METHOD:Spontaneous seizures (n = 6 animals) presented with either hypersynchronous- (HYP, n = 18) or low-voltage fast-onset (LVF, n = 21) pattern. Ripples and fast ripples overlapping by more than 30% were analysed. RESULTS:Overlapping HFOs could show a unimodal power spectrum between 80-500 Hz (n = 188, 58.9%) or a bimodal power spectrum, with peaks in power between 80 and 200 Hz and between 250 and 500 Hz (n = 131, 41.1%,). Overlapping HFOs occurred at higher rates during HYP seizures compared to the pre-ictal period in seizure onset zones (p < 0.001) and regions of secondary spread (p < 0.001). When comparing HYP and LVF seizures, we found that overlapping HFOs occurred at higher rates before LVF seizures (p < 0.05) compared to HYP seizures but, during the ictal period, HYP seizures showed higher rates of overlapping HFOs than LVF seizures (p < 0.001). COMPARISON WITH EXISTING METHODS:We have analysed overlapping ripples and fast ripples shortly before and during seizures. CONCLUSIONS:Although overlapping ripples and fast ripples represent a minority of HFOs, they may provide additional information on the excitability of neuronal networks that generate seizures in animal models and patients presenting with mesial temporal lobe epilepsy.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Lévesque M,Wang S,Gotman J,Avoli Mdoi
10.1016/j.jneumeth.2018.02.011subject
Has Abstractpub_date
2018-04-01 00:00:00pages
16-21eissn
0165-0270issn
1872-678Xpii
S0165-0270(18)30041-4journal_volume
299pub_type
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