Abstract:
:The biotin derivative, N-(2-aminoethyl) biotinamide hydrochloride, or Neurobiotin, has been shown recently to be a useful marker for intracellular and anterograde tracing. The properties of Neurobiotin as a tracer were further examined in this study by making pressure injections into different regions of the cerebral cortex or the rostral neostriatum of rats or by incubating striatal cells in culture with the tracer. Results showed extensive anterograde transport of Neurobiotin in cortical axons and terminals within the neostriatum 2-70 h after single or multiple cortical injections of the tracer. Similarly, profuse axonal projections to the medial portion of the globus pallidus were seen after an injection of Neurobiotin into the rostral neostriatum. Transneuronal labeling of medium-size neostriatal neurons was observed following injections of Neurobiotin into the prefrontal cortex. At the ultrastructural level, anterogradely labeled cortical axon terminals and transneuronally labeled neurons were readily identified in the caudate-putamen by the presence of both fine particulate and large punctate reaction products. Retrograde fillings of neurons resembling a Golgi-impregnation were seen in the ventral posterior complex of the thalamus after injections in the sensorimotor cortex. Neurons in the medial globus pallidus were also retrogradely labeled following tracer injections in the rostral caudate-putamen. Finally, Neurobiotin was readily and selectively taken up by striatal neurons in culture, where it extensively labeled somata and neurites. These results show that Neurobiotin is a versatile new tracer, which can be potentially useful for the study of neuronal organization in vivo and in vitro.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Huang Q,Zhou D,DiFiglia Mdoi
10.1016/0165-0270(92)90121-ssubject
Has Abstractpub_date
1992-01-01 00:00:00pages
31-43issue
1eissn
0165-0270issn
1872-678Xpii
0165-0270(92)90121-Sjournal_volume
41pub_type
杂志文章abstract::Neuromuscular electrical stimulation (NMES) can provide functional movements in people after central nervous system injury. The neuroplastic effects of long-term NMES-induced repetitive limb movement are not well understood. A rodent model of neurotrauma in which NMES can be implemented may be effective for such inves...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.09.015
更新日期:2009-01-30 00:00:00
abstract:BACKGROUND:Mixed frequency and phase coding (FPC) can achieve the significant increase of the number of commands in steady-state visual evoked potential-based brain-computer interface (SSVEP-BCI). However, the inconsistent phases of the SSVEP over channels in a trial and the existence of non-contributing channels due t...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.04.001
更新日期:2018-07-01 00:00:00
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.07.007
更新日期:2017-10-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.12.020
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.02.004
更新日期:2014-04-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2012.01.019
更新日期:2012-09-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(85)90122-0
更新日期:1985-07-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.01.014
更新日期:2008-05-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.07.022
更新日期:2010-09-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2015.11.008
更新日期:2016-02-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2015.12.007
更新日期:2016-05-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.09.005
更新日期:2011-01-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(83)90106-1
更新日期:1983-09-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.09.012
更新日期:2016-01-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.11.027
更新日期:2014-02-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.02.004
更新日期:2011-04-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.01.007
更新日期:2011-03-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(96)00158-6
更新日期:1997-03-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.10.006
更新日期:2016-12-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2019.108532
更新日期:2020-02-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(91)90178-3
更新日期:1991-07-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(87)90070-7
更新日期:1987-04-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.108419
更新日期:2019-12-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.04.002
更新日期:2015-07-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2014.10.007
更新日期:2015-01-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(01)00423-x
更新日期:2001-09-30 00:00:00
abstract::I describe here the successful visualization of interhemispheric callosal connections in the live mammalian cortex. The development of this method was prompted by the finding that fluorescent tracer labeling of groups of cortical neurons, when done under optimal conditions, is sufficiently intense to be visible even i...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(88)90137-9
更新日期:1988-10-01 00:00:00