Abstract:
:The lability of brain myosin-V (BM-V) to aldehyde-fixation has hindered immunohistochemical (IH) studies of this actin-based motor. We show here that BM-V immunoreactivity (IR) can be retrieved from formalin-fixed, paraffin-embedded human tissue. BM-V IR was optimally retrieved by boiling 5 microm cerebellar tissue sections in 10 mM sodium citrate buffer, pH 6, for 15 min, using a microwave oven set at 900 W and 2.45 GHz. A polyclonal, affinity purified anti-BM-V antibody, raised in rabbits against the tail domain of chicken BM-V, was shown here to recognize a single band in Western blots of human cortical homogenates. The combined use of this monospecific antibody and of the antigen retrieval (AR) method above allowed us to verify that BM-V IR is strongly expressed in human Purkinje cell bodies and dendrites, and in granule cells. The same pattern of BM-V IR expression was consistently and maximally detected in tissues stored in 10% formalin from 1 week to 2.5 months. The AR protocol for BM-V described here permits its IH study in formaldehyde-fixed tissues. It is a valuable tool to study BM-V in well fixed tissues, as occurs with the large collection of human archival tissue available.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Martins AR,Dias MM,Vasconcelos TM,Caldo H,Costa MC,Chimelli L,Larson REdoi
10.1016/s0165-0270(99)00090-4subject
Has Abstractpub_date
1999-10-15 00:00:00pages
25-9issue
1-2eissn
0165-0270issn
1872-678Xpii
S0165-0270(99)00090-4journal_volume
92pub_type
杂志文章abstract::A simple method is described for achieving a salt bridge within a patch pipette. The tip of the pipette is filled with a Cl(-)-free solution that bathes the membrane patch. Above this is a Cl(-)-containing solution that surrounds the recording Ag/AgCl wire electrode. Addition of 0.07% (w/v) agarose to the solution in ...
journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2013.06.001
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.09.009
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abstract:BACKGROUND:Electroencephalography (EEG) experiments often require several computers to ensure accurate stimulus presentation and data collection. However, this requirement can make it more difficult to perform such experiments in mobile settings within, or outside, the laboratory. NEW METHOD:Computer miniaturisation a...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2018.07.013
更新日期:2018-10-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00013-k
更新日期:1995-08-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.02.004
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.01.020
更新日期:2009-04-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.07.007
更新日期:2009-09-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00059-3
更新日期:1995-11-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.08.032
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
abstract::The glycogen-depletion technique has become a well-established method for determining histologically the cross-sectional distribution of a single muscle unit. A major drawback of this method is its low yield of one depleted unit per experiment. Furthermore, this technique is particularly unsuited for determining the l...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
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更新日期:1987-10-01 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.09.013
更新日期:2012-01-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2004.08.010
更新日期:2005-03-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.08.005
更新日期:2015-11-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.07.018
更新日期:2011-09-30 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.03.024
更新日期:2016-06-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
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更新日期:1992-06-01 00:00:00