Abstract：
:Mutant protein aggregates are an important biomarker in Huntington's and other neurodegenerative diseases however their quantification has typically relied on manual imaging and counting, or cell-free assays, which do not allow for concurrent analysis of cell viability. Here we describe four automated high throughput image analysis methods, developed using Metamorph software, to quantify mutant huntingtin aggregates in a cellular context. Imaging of aggregate-forming cells was also automated, using a Discovery-1 automated fluorescence microscope. All four analysis methods measured aggregate formation accurately in relation to manual counting, but with differing throughput. Our in-house PolyQ assay gave the highest throughput, processing images at 0.31 s per image. The Cell Scoring assay gave lower throughput, at 19.5s per image, but offered accurate quantification of the proportion of cells which formed aggregates, without bias from cell death. These image analysis tools provide rapid and objective alternatives to manual counting in studies of aggregate formation, to facilitate the discovery of drugs to treat Huntington's and related neurodegenerative diseases.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Scotter EL,Narayan P,Glass M,Dragunow Mdoi
10.1016/j.jneumeth.2008.02.007subject
Has Abstractpub_date
2008-06-15 00:00:00pages
174-9issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(08)00095-2journal_volume
171pub_type
杂志文章abstract：:Reach-to-grasp tasks have been used to study rodent models of motor system damage, stroke, and neurodegenerative disorders such as Parkinson's. These tasks are especially useful as they allow evaluation of bilateral or unilateral damage in different regions of the brain. Performing reach-to-grasp tests in juvenile mic...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.08.016
更新日期：2010-10-30 00:00:00
abstract：:We present a quantified imaging method that describes the cell column in mammalian cortex. The minicolumn is an ideal template with which to examine cortical organization because it is a basic unit of function, complete in itself, which interacts with adjacent and distance columns to form more complex levels of organi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00192-2
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abstract：:In order to improve cell therapy techniques, we have characterized a multipotent neural precursor cell isolation technique from the subventricular zone of adult pig brain. The pig is a non-primate species that is immunologically closest to human. The proliferative zone of this neurogenic structure was first localized ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.06.008
更新日期：2009-09-15 00:00:00
abstract：BACKGROUND:Electrophysiological recordings contain mixtures of signals from distinct neural sources, impeding a straightforward interpretation of the sensor-level data. This mixing is particularly detrimental when distinct sources resonate in overlapping frequencies. Fortunately, the mixing is linear and instantaneous....
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.109063
更新日期：2020-12-25 00:00:00
abstract：:Vibrotactile stimulation has been used successfully to activate the human somatosensory pathway in functional magnetic resonance imaging (fMRI) experiments. The design and characterization of these devices are of particular interest in frequency discrimination tasks and investigations of the somatopic organization of ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.07.018
更新日期：2009-10-30 00:00:00
abstract：:Evaluation of the amount of overall animal movement is important for investigations of motor control mechanisms in the central nervous system. We describe a new method to quantify overall free movements of an animal without any markers using a video camera and a personal computer equipped with a video-capture board. T...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(99)00082-5
更新日期：1999-09-15 00:00:00
abstract：:This review considers the different forms of reversible deactivation and how they differ from each other and from more conventional permanent deactivation methods. The different methods of chemical and cooling reversible inactivation and the limitations of each are discussed. Finally, future directions in the quest to...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/s0165-0270(98)00160-5
更新日期：1999-01-01 00:00:00
abstract：:Experimental evidence points to the importance of mitochondrial transport defects in contributing to major neurodegenerative diseases, such as Parkinson's disease (PD). Studies of mitochondrial transport along single axons are difficult with traditional dissociated culture systems and the fragility of the midbrain dop...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.05.021
更新日期：2012-07-30 00:00:00
abstract：:Non-invasive neuroimaging is increasingly used for investigating the human amygdala. Accurate functional localization in the amygdala region is, however, challenging and quantitative data on the anatomical specificity of functional amygdala imaging is lacking. We have therefore retrospectively investigated 114 recentl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.02.022
更新日期：2009-05-30 00:00:00
abstract：:Single, i.e. unconnected spinal or cerebral neurons from chick embryos rapidly regenerate morphologically identifiable axons and dendrites when cultured in a three-dimensional (3-D) extracellular matrix (ECM) consisting of a hydrated native collagen lattice. We now show that it is possible to study the intrinsic elect...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90052-5
更新日期：1987-07-01 00:00:00
abstract：:Behavioral recovery is the major goal of neuroscience research on the peripheral nervous system. However, behavioral methods of assessing peripheral nerve regeneration are still lacking. In the present work we used the median nerve and finger flexors as the experimental model. After resection or crush injury of the me...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00169-h
更新日期：1995-05-01 00:00:00
abstract：:A novel epineural tube implantation paradigm in the adult rat was designed for the analysis of regulatory cell interactions in peripheral nerves and for the development of therapeutic implants. The aim was to allow the integration of synthetic regenerative structures and cells into the nerve interior while preserving ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.04.007
更新日期：2010-06-15 00:00:00
abstract：:The incidence of leptomeningeal carcinomatosis is on the rise and current treatment modalities have limited efficacy. The development of new treatment strategies has been hampered by the lack of an appropriate animal model that accurately parallels the clinical condition. We have developed a new surgical technique for...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00115-0
更新日期：1998-11-01 00:00:00
abstract：:We developed a high speed voice coil based whisker stimulator that delivers precise deflections of a single whisker or group of whiskers in a repeatable manner. The device is miniature, quiet, and inexpensive to build. Multiple stimulators fit together for independent stimulation of four or more whiskers. The system c...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.12.008
更新日期：2011-03-15 00:00:00
abstract：:Cell transplantation therapy has raised a great interest in the perspective of its employment for nerve tissue repair. Among the various cell populations proposed, olfactory ensheathing glial cells have raised great interest over recent years, especially in the perspective of their employment for neural repair because...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.09.021
更新日期：2009-12-15 00:00:00
abstract：:We have developed a novel double-labeling method to investigate multiple gene expression in single cells. The method relies on the use of a radioactive probe followed by a colorimetric probe. Unique to this method, the radioactive signal is first captured on an emulsion pre-coated slide, which totally separates it fro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00163-8
更新日期：1996-06-01 00:00:00
abstract：:Generalized linear models (GLMs) have been developed for modeling and decoding population neuronal spiking activity in the motor cortex. These models provide reasonable characterizations between neural activity and motor behavior. However, they lack a description of movement-related terms which are not observed direct...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2010.03.024
更新日期：2010-06-15 00:00:00
abstract：BACKGROUND:The accuracy of source reconstruction depends on the spatial configuration of the neural sources underlying encephalographic signals, the temporal distance of the source activity, the level and structure of noise in the recordings, and - of course - on the employed inverse method. This plenitude of factors r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.02.016
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Neuronal cell cultures are widely used in the field of neuroscience. Cell dissociation allows for the isolation of a desired cell type, yet the neuronal complexity that distinguishes the nervous system is often lost as a result. Thus, culturing neural tissues in ex vivo format provides a physiological contex...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108546
更新日期：2020-02-15 00:00:00
abstract：:We examined topographic variations in nonlinear measures based on scalp voltages, which were generated by two simulated current dipoles each placed in a different hemisphere of a spherical volume conductor (three-shell model). Dipole dynamics were that of a three-torus and the x-component of the Lorenz-system and scal...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00188-6
更新日期：2000-05-15 00:00:00
abstract：BACKGROUND:Anatomically and physiologically, there is strong relationship between the brain and body. A new MRI platform covering both the brain and the limb would be beneficial for a more thorough understanding of the brain-body interactions. NEW METHOD:A new arm-over-head (AOH) position was developed to collect MRI ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108829
更新日期：2020-10-01 00:00:00
abstract：:This study aimed to explore the role of surface electromyography (sEMG) on the changes of myoelectric activities of quadriceps femoris and the assessment of its clinical effect before and after selective femoral neurotomy on spastic cerebral palsy with stiff knee. Selective femoral neurotomy was carried out in 15 pati...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.04.031
更新日期：2011-07-15 00:00:00
abstract：:The use of fluorescent calcium indicator, Indo-1, was evaluated for measuring changes in intracellular free calcium during electrical stimulation and anoxia in hippocampal slices. Fluorescence was measured from slices illuminated with brief (3 ns) light pulses (337 nm wavelength) from a nitrogen laser. This method of ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(89)90017-4
更新日期：1989-05-01 00:00:00
abstract：:Since pain is an important symptom in arthritis, useful behavioral indices for pain in experimental arthritis animal models are important tools for investigative work on arthritis. The purpose of this study was to develop simple and quantifiable behavioral tests, which would represent the level of arthritic pain that ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00011-0
更新日期：2002-03-30 00:00:00
abstract：:While it has long been recognized that humans recover function after stroke or head injury, the direct impact of drugs on recovery has only recently received attention. The ability of rats to traverse a narrow, elevated beam has proven to be particularly useful when studying the effects of pharmacologic manipulations ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90154-8
更新日期：1990-02-01 00:00:00
abstract：:Microdialysis coupled to liquid chromatography (LC) has proven to be a valuable in vivo sampling technique for studying neurotransmitter changes in normal and ischaemic brain. However, few analytical methods have described the simultaneous determination of amino acids, relevant in stroke research, together with the ni...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.10.013
更新日期：2005-05-15 00:00:00
abstract：:We used 24 sacral dorsal roots of the rat to analyze amplitude and area changes of biphasic and monophasic compound action potentials (CAPs) at 4 conduction distances. Both the CAPs of the A-fiber and C-fiber were analyzed. The changes were examined with the paired t test and linear regression. All the variables decre...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90064-x
更新日期：1993-03-01 00:00:00
abstract：:Controlled expression of proteins is a key experimental approach to a deeper understanding of the molecular basis of neuronal function. Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. We demonstrate that HSV-1 amplicon vectors expressing enhanced gree...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00262-5
更新日期：2002-12-15 00:00:00
abstract：:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90045-3
更新日期：1988-01-01 00:00:00
abstract：:The goal of the current study is to find a suitable classifier for electroencephalogram (EEG) data derived from a new learning paradigm which aims at communication in paralysis. A reflexive semantic classical (Pavlovian) conditioning paradigm is explored as an alternative to the operant learning paradigms, currently u...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.09.013
更新日期：2012-01-15 00:00:00
