Abstract：
:The use of fluorescent calcium indicator, Indo-1, was evaluated for measuring changes in intracellular free calcium during electrical stimulation and anoxia in hippocampal slices. Fluorescence was measured from slices illuminated with brief (3 ns) light pulses (337 nm wavelength) from a nitrogen laser. This method of illumination produced more intense fluorescence than illumination with light from filtered xenon or mercury arc lamps, and prevented loss of electrical excitability encountered following continuous UV illumination. Background fluorescence in control slices (without Indo-1) was considerable, often approaching 50% of that obtainable after dye loading. A more serious concern, however, was that a large fraction (approximately 10%) of the background fluorescence was labile to both electrical stimulation and anoxia. This fluorescence results from changes in the reduction/oxidation (redox) state of nicotinamide adenine dinucleotide (NAD), which fluoresces in its reduced (NADH) but not oxidized (NAD+) form. Qualitative changes in free calcium could be measured by first determining the ratio of change in NADH fluorescence at 405 and 485 nm (the wavelengths of light used to measure calcium with Indo-1) prior to dye loading. Any arbitrary baseline could be selected as long as the ratio for the baseline at 405 and 485 nm was identical to that determined for labile NADH. By application of this compensation procedure, it was determined that intracellular calcium rose abruptly during the onset of anoxia and again during the spreading depression-like loss of ion homeostasis which inevitably occurred during anoxia in these slices.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Sick TJ,Rosenthal Mdoi
10.1016/0165-0270(89)90017-4subject
Has Abstractpub_date
1989-05-01 00:00:00pages
125-32issue
1-2eissn
0165-0270issn
1872-678Xpii
0165-0270(89)90017-4journal_volume
28pub_type
杂志文章abstract：:The social interaction test in rats provides a method for detecting anxiolytic activity that does not use food or water deprivation, or electric shock, and therefore obviates difficulties of interpretation that might arise from drug-induced changes in motivation. Since social interaction is measured under more than on...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(80)90012-6
更新日期：1980-06-01 00:00:00
abstract：:A method is described for developing and evaluating models of neurochemical processes. Computer simulation and simplex optimization are used to examine a model of the dopaminergic nerve terminal of the rat striatum. In the model, synthesis, storage, release, uptake, and metabolism are described by a set of non-linear ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90045-3
更新日期：1988-01-01 00:00:00
abstract：:A new non-metallic push-pull cannula device is described for the localized perfusion of brain sites in the rat or other animal. Comprised entirely of glass and plastic materials, the cannula is intended for usage in an experiment in which the presence in tissue of metal tubing for delivery of perfusate is methodologic...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90129-2
更新日期：1983-10-01 00:00:00
abstract：:Neuromuscular electrical stimulation (NMES) can provide functional movements in people after central nervous system injury. The neuroplastic effects of long-term NMES-induced repetitive limb movement are not well understood. A rodent model of neurotrauma in which NMES can be implemented may be effective for such inves...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.09.015
更新日期：2009-01-30 00:00:00
abstract：:Vibrotactile stimulation has been used successfully to activate the human somatosensory pathway in functional magnetic resonance imaging (fMRI) experiments. The design and characterization of these devices are of particular interest in frequency discrimination tasks and investigations of the somatopic organization of ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.07.018
更新日期：2009-10-30 00:00:00
abstract：:Event-related potentials (ERPs) are evoked brain potentials that are averaged across many trial repetitions with individual trials aligned (i.e. time-locked) to a specific behavioral event, typically the onset of the stimulus (s-lock) or the onset of the behavioral response (r-lock). These evoked potential averages ma...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00194-5
更新日期：1998-03-13 00:00:00
abstract：:Changes in expression of phenotypic markers characterizing the cells comprising intact neurospheres are difficult to determine easily and accurately. The problem is compounded by the diversity of cell populations and phenotypes involved, and consequent non-uniform protein expression across the neurosphere wall, or aro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.04.014
更新日期：2004-10-15 00:00:00
abstract：:An interactive microcomputer system has been developed for the quantitative analysis of Golgi-impregnated neuronal morphology (GINM). The system uses commercially available hardware: a 48K RAM microcomputer (Apple II), a TV monitor (NEC), an optical microscope (Reichert Biovar) with a camera lucida and a specially dev...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90043-9
更新日期：1986-04-01 00:00:00
abstract：BACKGROUND:Multiscale entropy (MSE) estimates the predictability of a signal over multiple temporal scales. It has been recently applied to study brain signal variability, notably during aging. The grounds of its application and interpretation remain unclear and subject to debate. METHOD:We used both simulated and exp...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.09.004
更新日期：2016-11-01 00:00:00
abstract：BACKGROUND:Epileptic seizures evolve through several states, and in the process the brain signals may change dramatically. Signals from different states share similar features, making it difficult to distinguish them from a time series; the goal of this work is to build a classifier capable of identifying seizure state...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.12.001
更新日期：2015-02-15 00:00:00
abstract：BACKGROUND:Controlled cortical impact (CCI) has emerged as one of the most flexible and clinically applicable approaches for the induction of traumatic brain injury (TBI) in rodents and other species. Although this approach has been shown to model cognitive and functional outcomes associated with TBI in humans, recent ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.10.027
更新日期：2019-01-15 00:00:00
abstract：:Skeletal muscle is a convenient model system for studying basic questions on the neural regulation of synaptogenesis and of many properties of sarcolemma and contractile apparatus. The study of the neural signals involved in a particular regulation and of the mediating intracellular pathways, requires the chronic appl...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00352-6
更新日期：2001-04-30 00:00:00
abstract：:We describe a novel stimulus delivery system designed to present tactile stimuli to a subject in the tunnel of a magnetic resonance imaging (MRI) system. Using energy from an air-driven piston to turn a wheel, the device advances a conveyor belt with a pre-determined sequence of stimuli that differ in their spatial fe...
journal_title：Journal of neuroscience methods
pub_type： 临床试验,杂志文章
doi：10.1016/j.jneumeth.2006.07.020
更新日期：2007-01-30 00:00:00
abstract：BACKGROUND:Processing and quantitative analysis of magnetic resonance spectroscopy (MRS) data are far from standardized and require interfacing with third-party software. Here, we present Osprey, a fully integrated open-source data analysis pipeline for MRS data, with seamless integration of pre-processing, linear-comb...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108827
更新日期：2020-09-01 00:00:00
abstract：:The cost of electronic equipment can be a critical barrier to including neurophysiology exercises in biology teaching programs. We describe the construction of a simple and inexpensive AC preamplifier with performance comparable to that of commercial products. The amplifier consists of two integrated circuits in five ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00328-4
更新日期：2001-03-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.12.005
更新日期：2014-02-15 00:00:00
abstract：:We have developed a new method for culturing cells that maintains their health and sterility for many months. Using conventional techniques, primary neuron cultures seldom survive more than 2 months. Increases in the osmotic strength of media due to evaporation are a large and underappreciated contributor to the gradu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00412-5
更新日期：2001-09-30 00:00:00
abstract：:Analysis and interpretation of current records from cellular membranes or lipid bilayers is complicated by perturbations. These perturbations can originate from random noise or deterministic artifacts as capacitive currents caused by step depolarization's, drift, 50 Hz pick up or microphonics. Accurate removal of dete...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00057-0
更新日期：1998-08-01 00:00:00
abstract：BACKGROUND:Neurotrauma patients face major neurological sequelae. The failure in the preclinical-to-clinical translation of candidate therapies could be due to poor evaluation of rodent behaviours after neurotrauma. NEW METHOD:A home cage automated system was used to study the long term behaviour of individual rats wi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.04.005
更新日期：2019-06-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/S0165-0270(96)00101-X
更新日期：1996-12-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.09.012
更新日期：2016-01-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.109045
更新日期：2020-12-24 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.05.001
更新日期：2007-08-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90131-8
更新日期：1988-11-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.04.012
更新日期：2009-06-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.01.022
更新日期：2013-04-15 00:00:00
abstract：:Most techniques used for the study of the fiber connectivity in the central nervous system produce results which are visualized in the conventional light microscope or fluorescence microscope. Although in some cases this may be sufficient, often proof is necessary that fibers which enter a particular brain area indeed...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00300-9
更新日期：2000-11-15 00:00:00
abstract：:A reliable and economical silver staining method is recommended for demonstration of Lewy bodies (LB) and Lewy neurites (LN) in Parkinson's disease (PD) and of argyrophilic oligodendrocytes in multiple system atrophy (MSA). The technique can be applied to routinely formalin-fixed autopsy material and does not require ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00173-3
更新日期：1999-02-01 00:00:00
abstract：BACKGROUND:Currently the problem of incorporating priori information into an independent component analysis (ICA) model is often solved under the framework of constrained ICA, which utilizes the priori information as a reference signal to form a constraint condition and then introduce it into classical ICA. However, it...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.03.018
更新日期：2017-05-01 00:00:00
abstract：:Detecting brain structural changes from magnetic resonance (MR) images can facilitate early diagnosis and treatment of neurological and psychiatric diseases. Many existing methods require an accurate deformation registration, which is difficult to achieve and therefore prevents them from obtaining high accuracy. We de...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.09.001
更新日期：2014-01-15 00:00:00
