当前位置： SCI文献检索 > JOURNAL OF NEUROSCIENCE METHODS期刊下所有文献 > A retrograde double-labeling technique for light microscopy. A combination of axonal transport of cholera toxin B-subunit and a gold-lectin conjugate.

A retrograde double-labeling technique for light microscopy. A combination of axonal transport of cholera toxin B-subunit and a gold-lectin conjugate.

Abstract：

:A light microscopical, non-fluorescent, retrograde double-labeling technique is described. Cholera toxin B-subunit (CTb) and a conjugate of wheatgerm agglutinin and bovine serum albumin coupled to 10 nm gold particles (gold-lectin) are both excellent retrograde tracers and, when visualized by means of immunohistochemistry and silver intensification, respectively, may be readily identified within the same cell. This light microscopical retrograde double-labeling technique is illustrated in rat with experiments designed to investigate the collateralisation (1) of vestibular neurons to the spinal cord and oculomotor complex, (2) of spinal neurons to the left and right lateral reticular nucleus, and (3) of inferior olivary neurons to the uvula of the cerebellum. Advantages over fluorescent double-labeling experiments are found in the fact that the diaminobenzidine reaction product as well as the silver/gold deposits do not fade and can be examined in counterstained sections. Moreover, the injection sites can be kept quite small and may be guided by electrophysiological recording through the injection pipette.

journal_name

J Neurosci Methods

journal_title

Journal of neuroscience methods

authors

Ruigrok TJ,Teune TM,van der Burg J,Sabel-Goedknegt H

doi

10.1016/0165-0270(94)00034-e

subject

Has Abstract

pub_date

1995-09-01 00:00:00

pages

127-38

issue

1-2

eissn

0165-0270

issn

1872-678X

pii

0165-0270(94)00034-E

journal_volume

61

pub_type

杂志文章

相关文献

JOURNAL OF NEUROSCIENCE METHODS文献大全
  • Resolution-based spectral clustering for brain parcellation using functional MRI.

    abstract：BACKGROUND:Brain parcellation is important for exploiting neuroimaging data. Variability in physiology between individuals has led to the need for data-driven approaches to parcellation, with recent research focusing on simultaneously estimating and partitioning the network structure of the brain. NEW METHOD:We view d...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2020.108628

    authors： Dillon K,Wang YP

    更新日期：2020-04-01 00:00:00

  • The effects of physiologically plausible connectivity structure on local and global dynamics in large scale brain models.

    abstract：:Functionally relevant large scale brain dynamics operates within the framework imposed by anatomical connectivity and time delays due to finite transmission speeds. To gain insight on the reliability and comparability of large scale brain network simulations, we investigate the effects of variations in the anatomical ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2009.07.007

    authors： Knock SA,McIntosh AR,Sporns O,Kötter R,Hagmann P,Jirsa VK

    更新日期：2009-09-30 00:00:00

  • Quantification of antioxidant activity in brain tissue homogenates using the 'total equivalent antioxidant capacity'.

    abstract：:We demonstrate herein that the standard 'Trolox equivalent antioxidant capacity' (TEAC) assay, typically utilized to quantify total antioxidant levels within plasma, can also be utilized for tissue homogenates. Normal mice and transgenic mice lacking apolipoprotein E were subjected to a diet including iron as a generi...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(03)00028-1

    authors： Shea TB,Rogers E,Ashline D,Ortiz D,Sheu MS

    更新日期：2003-05-30 00:00:00

  • Abnormal cortical region and subsystem complexity in dynamical functional connectivity of chronic schizophrenia: A new graph index for fMRI analysis.

    abstract：OBJECTIVES:Schizophrenia is a predominant product of pathological alterations distributed throughout interconnected neural systems. Designing new objectively diagnostic methods are burning questions. Dynamical functional connectivity (DFCs) methodology based on fMRI data is an effective lever to investigate changeabili...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2018.10.011

    authors： Chen B

    更新日期：2019-01-01 00:00:00

  • Rapid analysis of GABA and glutamate in microdialysis samples using high performance liquid chromatography and tandem mass spectrometry.

    abstract：:A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method has been established for the rapid and reliable determination of gamma-aminobutyric acid (GABA) and glutamate in brain microdialysates. The microdialysis samples were analysed using a HILIC (hydrophilic interaction liquid chromatography) column, which ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2009.05.018

    authors： Buck K,Voehringer P,Ferger B

    更新日期：2009-08-30 00:00:00

  • Time-frequency component analyser and its application to brain oscillatory activity.

    abstract：:Currently, event-related potential (ERP) signals are analysed in the time domain (ERP technique) or in the frequency domain (Fourier analysis and variants). In techniques of time-domain and frequency-domain analysis (short-time Fourier transform, wavelet transform) assumptions concerning linearity, stationarity, and t...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2004.12.003

    authors： Ozdemir AK,Karakaş S,Cakmak ED,Tüfekçi DI,Arikan O

    更新日期：2005-06-30 00:00:00

  • Neurophysiology of neocortical slices resected from children undergoing surgical treatment for epilepsy.

    abstract：:The recent emergence of surgical treatment of childhood epilepsy has led to the accessibility of young human cerebral tissue for electrophysiological studies of the mechanisms involved in epileptogenesis. Intracellular recordings were obtained from neurons in slices prepared from neocortical tissue resected from child...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(94)00193-k

    authors： Dudek FE,Wuarin JP,Tasker JG,Kim YI,Peacock WJ

    更新日期：1995-06-01 00:00:00

  • The extended application of The Rat Brain in Stereotaxic Coordinates in rats of various body weight.

    abstract：BACKGROUND:Stereotaxic procedures in rats are frequently used in neurological research. The commonly used rat brain stereotaxic atlases areThe Rat Brain in Stereotaxic Coordinates, the second most cited book in science, and Stereotaxic Atlas of Rat Brain (in Chinese) which based on Wistar rats of 290 g and SD rats of 2...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2018.06.026

    authors： Yang P,Wang Z,Zhang Z,Liu D,Manolios EN,Chen C,Yan X,Zuo W,Chen N

    更新日期：2018-09-01 00:00:00

  • A rapid cell counting method utilising acridine orange as a novel discriminating marker for both cultured astrocytes and microglia.

    abstract：:Cell culture analyses of growth, morphology and apoptosis commonly require counting of different cell types stained with antibodies to discriminate between them. Previously, we reported the use of l-Leucine methyl ester (l-LME) to prepare purified cultures of type 1 astrocytes with minimal microglia, and staining by G...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2007.06.009

    authors： Lovelace MD,Cahill DM

    更新日期：2007-09-30 00:00:00

  • Use of fNIRS to assess resting state functional connectivity.

    abstract：:Recently, resting state functional connectivity (RSFC) studies based on fMRI and EEG/MEG have provided valuable insight into the intrinsic functional architecture of the human brain. However, whether functional near infrared spectroscopy (fNIRS), a suitable imaging method for infant and patient populations, can be use...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2009.11.010

    authors： Lu CM,Zhang YJ,Biswal BB,Zang YF,Peng DL,Zhu CZ

    更新日期：2010-02-15 00:00:00

  • Synaptic currents recorded from the dendritic field of an insect giant interneurone.

    abstract：:A method is described enabling the recording of synaptic currents from an isolated interganglionic interneurone in the central nervous system of the cockroach, Periplaneta americana, using an oil-gap recording system. The circuitry contains a Wheatstone bridge in which the preparation is the unknown resistance. Using ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(88)90146-x

    authors： Mony L,Hue B,Tessier JC

    更新日期：1988-09-01 00:00:00

  • A simple electronic unit allowing extracellular recording and stimulation through the same wire hook or suction electrode.

    abstract：:A simple and easily constructed unit was designed using a buffer amplifier, a programmable transconductance amplifier and an active filter. The unit allows extracellular recording and stimulation through the same electrode. Thus in experiments where it is required to stimulate a nerve and record the effects of the sti...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(84)90034-7

    authors： Hatzopoulos A,Theophilidis G

    更新日期：1984-08-01 00:00:00

  • Hidden Markov modeling of frequency-following responses to Mandarin lexical tones.

    abstract：BACKGROUND:The frequency-following response (FFR) is a scalp-recorded electrophysiological potential reflecting phase-locked activity from neural ensembles in the auditory system. The FFR is often used to assess the robustness of subcortical pitch processing. Due to low signal-to-noise ratio at the single-trial level, ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2017.08.010

    authors： Llanos F,Xie Z,Chandrasekaran B

    更新日期：2017-11-01 00:00:00

  • In vivo chronoamperometric measurements of the clearance of exogenously applied serotonin in the rat dentate gyrus.

    abstract：:The present study evaluated high-speed chronoamperometry as a method for measuring the clearance of serotonin (5-HT) from extracellular space in vivo. Male Sprague-Dawley rats were anaesthetized and a Nafion-coated, carbon fiber electrode, attached to a multibarrel pipette, was lowered into the subgranular layer of th...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(97)00144-1

    authors： Daws LC,Toney GM,Davis DJ,Gerhardt GA,Frazer A

    更新日期：1997-12-30 00:00:00

  • A method for better positioning bipolar electrodes for lower limb EMG recordings during dynamic contractions.

    abstract：:To obtain a high quality EMG acquisition, the signal must be recorded as far away as possible from muscle innervations and tendon zones, which are known to shift during dynamic contractions. This study describes a methodology, using commercial bipolar electrodes, to identify better electrode positions for superficial ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2009.02.017

    authors： Sacco IC,Gomes AA,Otuzi ME,Pripas D,Onodera AN

    更新日期：2009-05-30 00:00:00

  • A novel mouse model of contralateral C7 transfer via the pretracheal route: A feasibility study.

    abstract：BACKGROUND:Contralateral seventh cervical nerve transfer (contralateral C7 transfer) is a novel treatment for patients with spastic paralysis, including stroke and traumatic brain injury. However, little is known on changes in plasticity that occur in the intact hemisphere after C7 transfer. An appropriate surgical mod...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2019.108445

    authors： Ye X,Shen YD,Feng JT,Wang F,Gao ZR,Lei GW,Yu AP,Wang CP,Liang CM,Xu WD

    更新日期：2019-12-01 00:00:00

  • Integration of confocal and atomic force microscopy images.

    abstract：:Atomic force microscopy (AFM) provides the possibility to map the 3D structure of viewed objects with a nanometric resolution, which cannot be achieved with other imaging methods such as conventional video imaging and confocal fluorescent microscopy. Video imaging with CCD cameras can provide an analysis of biological...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2008.09.034

    authors： Kondra S,Laishram J,Ban J,Migliorini E,Di Foggia V,Lazzarino M,Torre V,Ruaro ME

    更新日期：2009-02-15 00:00:00

  • Assessment of executive function in adolescence: a comparison of traditional and virtual reality tools.

    abstract：:Paper-pencil type tests are traditionally used in the assessment of executive functions (EF); however, concerns have been raised as to whether these represent actual functioning in everyday life. Virtual reality (VR) environments offer a novel alternative for the assessment of cognitive function and therefore have the...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2013.07.005

    authors： Lalonde G,Henry M,Drouin-Germain A,Nolin P,Beauchamp MH

    更新日期：2013-09-30 00:00:00

  • New methods of dye application for staining motor neurons in an insect.

    abstract：:Motor neurons innervating tiny muscles in the antennal system of the cockroach, Periplaneta americana, were stained by two new approaches to backfilling motor axons and somata. Both methods are easier, and produce less leakage of dye, than conventional methods that rely on application of dye to the distal cut end of a...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(00)00203-x

    authors： Baba Y

    更新日期：2000-06-01 00:00:00

  • A sequential protocol combining dual neuroanatomical tract-tracing with the visualization of local circuit neurons within the striatum.

    abstract：:We describe here an experimental approach designed to aid in the identification of complex brain circuits within the rat corpus striatum. Our aim was to characterize in a single section (i) striatal thalamic afferents, (ii) striatopallidal projection neurons and (iii) striatal local circuit interneurons. To this end, ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(01)00440-x

    authors： Gonzalo N,Moreno A,Erdozain MA,García P,Vázquez A,Castle M,Lanciego JL

    更新日期：2001-10-15 00:00:00

  • On the use of ibotenic acid to lesion selectively different components of the hippocampal formation.

    abstract：:Procedures are described for lesioning two components of the hippocampal formation (hippocampus, subiculum) using multiple injections of small amounts of ibotenic acid (IBO). The resulting loss of cells is more selective and limited than can be obtained with conventional techniques. Thus, problems associated with dama...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(89)90149-0

    authors： Jarrard LE

    更新日期：1989-09-01 00:00:00

  • Removing non-random artifacts from patch clamp traces.

    abstract：:Analysis and interpretation of current records from cellular membranes or lipid bilayers is complicated by perturbations. These perturbations can originate from random noise or deterministic artifacts as capacitive currents caused by step depolarization's, drift, 50 Hz pick up or microphonics. Accurate removal of dete...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(98)00057-0

    authors： Baumgartner W,Schindler H,Romanin C

    更新日期：1998-08-01 00:00:00

  • Contour-propagation algorithms for semi-automated reconstruction of neural processes.

    abstract：:A new technique, "serial block face scanning electron microscopy" (SBFSEM), allows for automatic sectioning and imaging of biological tissue with a scanning electron microscope. Image stacks generated with this technology have a resolution sufficient to distinguish different cellular compartments, including synaptic s...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2007.07.021

    authors： Macke JH,Maack N,Gupta R,Denk W,Schölkopf B,Borst A

    更新日期：2008-01-30 00:00:00

  • A biplanar slice preparation for studying cerebellar synaptic transmission.

    abstract：:Unlike certain other brain areas, notably the hippocampal formation, synaptic transmission in the cerebellum, a 3-dimensional anatomical structure, is no ideally studied in a 2-dimensional slice preparation. We describe a method for cutting a cerebellar slice that incorporates two planes of section: one sagittal (in t...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(95)00133-6

    authors： Garthwaite J,Batchelor AM

    更新日期：1996-02-01 00:00:00

  • Assessing mouse behaviour throughout the light/dark cycle using automated in-cage analysis tools.

    abstract：:An important factor in reducing variability in mouse test outcomes has been to develop assays that can be used for continuous automated home cage assessment. Our experience has shown that this has been most evidenced in long-term assessment of wheel-running activity in mice. Historically, wheel-running in mice and oth...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2017.04.014

    authors： Bains RS,Wells S,Sillito RR,Armstrong JD,Cater HL,Banks G,Nolan PM

    更新日期：2018-04-15 00:00:00

  • Long-term recordings and receptive field measurements from single units of the visual cortex of awake unrestrained kittens.

    abstract：:A method is described which allows simultaneous recording of the activity of several neurons in the visual cortex of awake unrestrained kittens for a period sufficiently long to follow experience-dependent changes in receptive field properties. The electrodes consisted of 25 microns thick Teflon-coated platinum-iridiu...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/0165-0270(88)90131-8

    authors： Mioche L,Singer W

    更新日期：1988-11-01 00:00:00

  • A microdevice platform for visualizing mitochondrial transport in aligned dopaminergic axons.

    abstract：:Experimental evidence points to the importance of mitochondrial transport defects in contributing to major neurodegenerative diseases, such as Parkinson's disease (PD). Studies of mitochondrial transport along single axons are difficult with traditional dissociated culture systems and the fragility of the midbrain dop...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2012.05.021

    authors： Lu X,Kim-Han JS,O'Malley KL,Sakiyama-Elbert SE

    更新日期：2012-07-30 00:00:00

  • Monitoring deep brain stimulation by measuring regional brain oxygen responses in freely moving mice.

    abstract：BACKGROUND:Translational studies investigating the effects of deep brain stimulation (DBS) on brain function up to now mainly relied on BOLD responses measured with fMRI. However, fMRI studies in rodents face technical and practical limitations (e.g., immobilization, sedation or anesthesia, spatial and temporal resolut...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2019.01.012

    authors： Bazzu G,Serra PA,Hamelink R,Feenstra MGP,Willuhn I,Denys D

    更新日期：2019-04-01 00:00:00

  • Spike sorting based on discrete wavelet transform coefficients.

    abstract：:Using the novel mathematical technique known as wavelet analysis, a new method (WSC) is presented to sort spikes according to a decomposition of neural signals in the time-frequency space. The WSC method is implemented by a pyramidal algorithm that acts upon neural signals as a bank of quadrature mirror filters. This ...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/s0165-0270(00)00250-8

    authors： Letelier JC,Weber PP

    更新日期：2000-09-15 00:00:00

  • Performance of juvenile mice in a reach-to-grasp task.

    abstract：:Reach-to-grasp tasks have been used to study rodent models of motor system damage, stroke, and neurodegenerative disorders such as Parkinson's. These tasks are especially useful as they allow evaluation of bilateral or unilateral damage in different regions of the brain. Performing reach-to-grasp tests in juvenile mic...

    journal_title：Journal of neuroscience methods

    pub_type： 杂志文章

    doi：10.1016/j.jneumeth.2010.08.016

    authors： Marques JM,Olsson IA

    更新日期：2010-10-30 00:00:00