Abstract：
:Motor neurons innervating tiny muscles in the antennal system of the cockroach, Periplaneta americana, were stained by two new approaches to backfilling motor axons and somata. Both methods are easier, and produce less leakage of dye, than conventional methods that rely on application of dye to the distal cut end of a motor nerve or immersion of a whole muscle in dye. One approach used here was to pressure-inject nickel (or cobalt) chloride directly into a muscle from a micropipette. The other was to place filter paper soaked in nickel chloride on the muscle surface. The first approach enabled staining of motor neurons most reliably and with preservation of stain to the specific muscle of interest. The second approach had different advantages: it was easier to perform, resulted in more intense staining and frequently more complete staining of dendritic structure than the first approach. Taken together, these two approaches should prove useful for tracing motor circuits in a number of model systems.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Baba Ydoi
10.1016/s0165-0270(00)00203-xsubject
Has Abstractpub_date
2000-06-01 00:00:00pages
165-9issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(00)00203-Xjournal_volume
98pub_type
杂志文章abstract：:One of the core symptoms of anxiety disorders, such as post-traumatic stress disorder, is the failure to overcome feelings of danger despite being in a safe environment. This deficit likely stems from an inability to fully process safety signals, which are cues in the environment that enable healthy individuals to ove...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2012-02-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.10.012
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2015-04-30 00:00:00
