Death of photoreceptors in organotypic retinal explant cultures: implication of rhodopsin accumulation and endoplasmic reticulum stress.


:Here we suggest that endoplasmic reticulum (ER)-stress may be induced following aberrant rhodopsin accumulation in photoreceptors in explanted rat retinas. Rhodopsin accumulation was accompanied by increased phosphorylation of pancreatic ER-kinase and eukaryotic initiator factor 2α as well as increased levels of C/EBP homologous protein, glucose-regulated protein 78 and eventually increased cleaved caspase-12 and cleaved caspase-3. Glucose-regulated protein 78, pancreatic ER-kinase, caspase-12 and cleaved caspase-3 were present in photoreceptors, indicating that ER-stress and apoptosis are induced in this cell population. These results suggest that ER-stress and subsequent apoptosis is induced in healthy photoreceptors, presumably by aberrant accumulation of rhodopsin and the phosphorylation of eukaryotic initiator factor 2α. The explant culture system may allow investigations of neuroprotective strategies.


J Neurosci Methods


Mohlin C,Johansson K




Has Abstract


2011-04-15 00:00:00














  • Extracellular perfusion controller for electrophysiology.

    abstract::This paper describes the design and utility of a perfusion controller to facilitate the handling of multiple extracellular solutions. The controller consists of an interface box with both manual and computer controls and a set of solenoid valves. This provides remote handling of the switching of multiple solutions int...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Stimers JR

    更新日期:1992-07-01 00:00:00

  • Clearing techniques for visualizing the nervous system in development, injury, and disease.

    abstract::Modern clearing techniques enable high resolution visualization and 3D reconstruction of cell populations and their structural details throughout large biological samples, including intact organs and even entire organisms. In the past decade, these methods have become more tractable and are now being utilized to provi...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Porter DDL,Morton PD

    更新日期:2020-01-13 00:00:00

  • Randomising stimulus intensity improves the variability and reliability of the assessment of corticospinal excitability.

    abstract:BACKGROUND:Advances in the control of transcranial magnetic stimulation (TMS) have enabled greater randomisation of stimulus intensity. It is unclear if such randomisation improves assessments of corticospinal excitability. NEW METHOD:We recorded the amplitude of TMS-induced motor evoked potentials (MEPs) from the fir...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Suckley JJ,Waters TJ,Tran M,Stapley PJ,Shemmell J,Walsh JA,McAndrew DJ

    更新日期:2020-08-01 00:00:00

  • Antidromic excitation of motoneurons by intramuscular electrical stimulation.

    abstract::The terminal branches of motoneurons can be excited by electrical current pulses delivered via needles inserted intramuscularly. Observations are reported which indicate that tungsten semi-microelectrodes suitably positioned in a muscle belly can excite antidromically a large proportion of the alpha-motoneurons of tha...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Muir RB,Lemon RN

    更新日期:1983-05-01 00:00:00

  • Physiological monitoring of small animals during magnetic resonance imaging.

    abstract::Maintaining a stable physiologic state is essential when studying animal models of epilepsy with simultaneous electroencephalograph (EEG) and functional magnetic resonance imaging (fMRI) or EEG and magnetic resonance spectroscopy (MRS). To achieve and maintain such stability in rats in the MRI environment, a minimally...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mirsattari SM,Bihari F,Leung LS,Menon RS,Wang Z,Ives JR,Bartha R

    更新日期:2005-06-15 00:00:00

  • Neuroanatomical tracing at high resolution.

    abstract::Most techniques used for the study of the fiber connectivity in the central nervous system produce results which are visualized in the conventional light microscope or fluorescence microscope. Although in some cases this may be sufficient, often proof is necessary that fibers which enter a particular brain area indeed...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Van Haeften T,Wouterlood FG

    更新日期:2000-11-15 00:00:00

  • Silver staining method for demonstrating Lewy bodies in Parkinson's disease and argyrophilic oligodendrocytes in multiple system atrophy.

    abstract::A reliable and economical silver staining method is recommended for demonstration of Lewy bodies (LB) and Lewy neurites (LN) in Parkinson's disease (PD) and of argyrophilic oligodendrocytes in multiple system atrophy (MSA). The technique can be applied to routinely formalin-fixed autopsy material and does not require ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Braak E,Braak H

    更新日期:1999-02-01 00:00:00

  • Measurements of behavior in the naked mole-rat after intraperitoneal implantation of a radio-telemetry system.

    abstract::The naked mole-rat (Heterocephalus glaber) is a unique fossorial mammal that is eusocial, object blind, and virtually poikilothermic. This animal is an ideal model to examine questions pertaining to the thermoregulatory aspects of sleep/wake cycles and other circadian events. However, the monitoring of mammalian sleep...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Herold N,Spray S,Horn T,Henriksen SJ

    更新日期:1998-06-01 00:00:00

  • PG25, a pineal-specific cDNA, cloned by differential display PCR (DDPCR) and rapid amplification of cDNA ends (RACE).

    abstract::Synthesis of melatonin in the mammalian pineal gland is regulated by the rhythmic expression of acetyl-CoA: serotonin N-acetyltransferase (SNAT) and other unknown factors. To screen for pineal-specific mRNAs potentially involved in melatonin synthesis and/or regulation, differential display PCR (DDPCR) was employed. W...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wang X,Brownstein MJ,Young WS

    更新日期:1997-05-16 00:00:00

  • A multichannel hook electrode for simultaneous recording of up to 12 nerve filaments.

    abstract::This paper describes a simple multichannel hook electrode specially adapted for simultaneous recording from several dorsal root filaments in the cat. The electrode consists of a 3-mm-thick PVC plate and accommodates 12 silver wire hooks placed in notches in the plate. It has a semicircular shape, and the notches are m...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Djupsjöbacka M,Johansson H,Bergenheim M,Sandström U

    更新日期:1994-04-01 00:00:00

  • Automated and quantitative image analysis of ischemic dendritic blebbing using in vivo 2-photon microscopy data.

    abstract::Ischemia induces a 'blebbing' of dendrites, a structural alteration where dendrites take on a 'beads on a string' appearance. We developed a toolkit program, BlebQuant, for quantitative automated bleb analysis to chart the morphology of dendrites labeled with GFP/YFP under normal conditions and after ischemia-induced ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Chen S,Tran S,Sigler A,Murphy TH

    更新日期:2011-02-15 00:00:00

  • A Cartesian coordinate system for human cerebral cortex.

    abstract::The most commonly used method for specifying the locations of functional areas in the human cerebral cortex is the coordinate system of Talairach and Tournoux (Co-planar Stereotaxic Altas of The Human Brain (1988) Georg Thieme Verlag, Stuttgart). It was designed to locate subcortical nuclei by reference to an axis joi...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Li DF,Freeman AW,Tran-Dinh H,Morris JG

    更新日期:2003-05-30 00:00:00

  • Rabies virus-mediated connectivity tracing from single neurons.

    abstract::An understanding of how the brain processes information requires knowledge of its underlying wiring diagrams, as well as insights into the relationship between circuit architecture and physiological function. Notably, rabies virus based single-cell genetic manipulations that can facilitate an experimental link between...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Schwarz MK,Remy S

    更新日期:2019-09-01 00:00:00

  • Volumetric imaging of brain activity with spatial-frequency decoding of neuromagnetic signals.

    abstract:BACKGROUND:The brain generates signals in a wide frequency range (∼2840 Hz). Existing magnetoencephalography (MEG) methods typically detect brain activity in a median-frequency range (1-70 Hz). The objective of the present study was to develop a new method to utilize the frequency signatures for source imaging. NEW ME...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Xiang J,Korman A,Samarasinghe KM,Wang X,Zhang F,Qiao H,Sun B,Wang F,Fan HH,Thompson EA

    更新日期:2015-01-15 00:00:00

  • An integrated system for synchronous detection of neuron spikes and dopamine activities in the striatum of Parkinson monkey brain.

    abstract:BACKGROUND:Synchronous detecting neuron spikes and dopamine (DA) activities in the non-human primate brain play an important role in understanding of Parkinson's disease (PD). At present, most experiments are carried out by combing of electrodes and commercial instruments, which are inconvenient, time-consuming and ine...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Xu S,Zhang Y,Zhang S,Xiao G,Wang M,Song Y,Gao F,Li Z,Zhuang P,Chan P,Tao G,Yue F,Cai X

    更新日期:2018-07-01 00:00:00

  • Preprocessing effects of 22 linear univariate features on the performance of seizure prediction methods.

    abstract::Combining multiple linear univariate features in one feature space and classifying the feature space using machine learning methods could predict epileptic seizures in patients suffering from refractory epilepsy. For each patient, a set of twenty-two linear univariate features were extracted from 6 electroencephalogra...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Rasekhi J,Mollaei MR,Bandarabadi M,Teixeira CA,Dourado A

    更新日期:2013-07-15 00:00:00

  • Estimation of volume fractions in nervous tissue with an image analyzer.

    abstract::The calculation of volume fractions in nervous tissue is a method often used in neuroanatomy. In ontogenetic studies and problems concerning aging, as well as after experimental procedures, volume fractions are calculated in order to quantify the results. Grey level index (GLI) and grey cell coefficient (GCC) are diff...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wree A,Schleicher A,Zilles K

    更新日期:1982-07-01 00:00:00

  • Turbulence in globus pallidum neurons in patients with Parkinson's disease: exponential decay of the power spectrum.

    abstract::Exponential decay of the power spectrum is typically observed in turbulent flow patterns, as evidenced both in experiments and in the Navier-Stokes equations describing fluid dynamics. In this study, we present evidence on the exponential decay of power spectra belonging to interspike interval time series, registered ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Andres DS,Cerquetti DF,Merello M

    更新日期:2011-04-15 00:00:00

  • A weighted index of bilateral brain lesions.

    abstract::Attempts to correlate the amount of bilateral brain injury with behavioral changes can be complicated if the lesions are bilaterally unsymmetrical in volume. Further complications are introduced if unilateral lesions are ineffective in producing behavioral changes. An index, W%, is proposed which is based on the produ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hodos W,Bobko P

    更新日期:1984-11-01 00:00:00

  • Classification of methods in transcranial electrical stimulation (tES) and evolving strategy from historical approaches to contemporary innovations.

    abstract::Transcranial Electrical Stimulation (tES) encompasses all methods of non-invasive current application to the brain used in research and clinical practice. We present the first comprehensive and technical review, explaining the evolution of tES in both terminology and dosage over the past 100 years of research to prese...

    journal_title:Journal of neuroscience methods

    pub_type: 历史文章,杂志文章,评审


    authors: Guleyupoglu B,Schestatsky P,Edwards D,Fregni F,Bikson M

    更新日期:2013-10-15 00:00:00

  • Direct transdifferentiation of human Wharton's jelly mesenchymal stromal cells into cholinergic-like neurons.

    abstract::Barckground Alzheimer's disease (AD) is mainly caused by cellular loss and dysfunction of the basal forebrain cholinergic neurons and cholinergic axons in the cortex leading to slowly progressive decline in learning and memory performance. Unfortunately, no definitive treatment to halt neural cell loss exists to date....

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mendivil-Perez M,Velez-Pardo C,Jimenez-Del-Rio M

    更新日期:2019-01-15 00:00:00

  • Responses of rats to noxious mechanical stimulation of their tails during tail reperfusion following transient ischaemia.

    abstract::We investigated whether conscious rats exhibit hyperalgesia to noxious mechanical stimuli during tail reperfusion following transient tail ischaemia. Mechanical stimuli were delivered by three mechanical algometers, differing in contact area, geometry and rate of application. Tail ischaemia was induced by applying a p...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Vidulich L,Mitchell D

    更新日期:2000-11-30 00:00:00

  • Laminin immunohistochemistry: a simple method to visualize and quantitate vascular structures in the mammalian brain.

    abstract::Immunohistochemistry using antiserum against the basement membrane glycoprotein laminin, was shown to be an excellent marker for brain blood vessels. Throughout the brain of mice, rats, guinea pigs, monkeys and humans, the basement membrane of the vascular structures were strongly laminin-positive. The neuropil itself...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Eriksdotter-Nilsson M,Björklund H,Olson L

    更新日期:1986-09-01 00:00:00

  • Replating improves whole cell voltage clamp recording of human fetal dorsal root ganglion neurons.

    abstract::The whole cell patch clamp technique allows recording of membrane currents in an entire cell under voltage clamp conditions. However, technical difficulties arise in large cells bearing extensive processes, such as human fetal dorsal root ganglion (DRG) neurons in culture. In order to improve space clamp conditions, h...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Caviedes P,Ault B,Rapoport SI

    更新日期:1990-10-01 00:00:00

  • Social behavior of zebrafish: from synthetic images to biological mechanisms of shoaling.

    abstract::The zebrafish strikes a good balance between system complexity and practical simplicity and as a result it is becoming increasingly frequently utilized in biomedical research as a translational tool. Numerous human brain disorders are associated with abnormal social behavior and the zebrafish has been suggested for mo...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Gerlai R

    更新日期:2014-08-30 00:00:00

  • Stimulation of Ca²+ signals in neurons by electrically coupled electrolyte-oxide-semiconductor capacitors.

    abstract::Electrolyte-oxide-semiconductor capacitors (EOSCs) are a class of microtransducers for extracellular electrical stimulation that have been successfully employed to activate voltage-dependent sodium channels at the neuronal soma to generate action potentials in vitro. In the present work, we report on their use to cont...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Giacomello M,Girardi S,Scorzeto M,Peruffo A,Maschietto M,Cozzi B,Vassanelli S

    更新日期:2011-05-15 00:00:00

  • Long-term recordings and receptive field measurements from single units of the visual cortex of awake unrestrained kittens.

    abstract::A method is described which allows simultaneous recording of the activity of several neurons in the visual cortex of awake unrestrained kittens for a period sufficiently long to follow experience-dependent changes in receptive field properties. The electrodes consisted of 25 microns thick Teflon-coated platinum-iridiu...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mioche L,Singer W

    更新日期:1988-11-01 00:00:00

  • Mitochondrial DNA depletion analysis by pseudogene ratioing.

    abstract::The mitochondrial DNA (mtDNA) depletion status of rho(0) cell lines is typically assessed by hybridization or polymerase chain reaction (PCR) experiments, in which the failure to hybridize mtDNA or amplify mtDNA using mtDNA-directed primers suggests thorough mitochondrial genome removal. Here, we report the use of an ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Swerdlow RH,Redpath GT,Binder DR,Davis JN 2nd,VandenBerg SR

    更新日期:2006-01-30 00:00:00

  • Quantitative analysis of acetylcholine in rat brain microdialysates by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.

    abstract::A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-β-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Carrozzo MM,Cannazza G,Pinetti D,Di Viesti V,Battisti U,Braghiroli D,Parenti C,Baraldi M

    更新日期:2010-12-15 00:00:00

  • Directly applied low intensity direct electric current enhances peripheral nerve regeneration in rats.

    abstract::The influence of direct electric stimulation on nerve regeneration was studied in a model of crush injury of the sciatic nerve of rats. Forty-three rats were used and distributed in four groups according to the procedure: (1) intact nerve, inactive circuit; (2) crush injury, inactive circuit; (3) intact nerve, active ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mendonça AC,Barbieri CH,Mazzer N

    更新日期:2003-10-30 00:00:00