Death of photoreceptors in organotypic retinal explant cultures: implication of rhodopsin accumulation and endoplasmic reticulum stress.


:Here we suggest that endoplasmic reticulum (ER)-stress may be induced following aberrant rhodopsin accumulation in photoreceptors in explanted rat retinas. Rhodopsin accumulation was accompanied by increased phosphorylation of pancreatic ER-kinase and eukaryotic initiator factor 2α as well as increased levels of C/EBP homologous protein, glucose-regulated protein 78 and eventually increased cleaved caspase-12 and cleaved caspase-3. Glucose-regulated protein 78, pancreatic ER-kinase, caspase-12 and cleaved caspase-3 were present in photoreceptors, indicating that ER-stress and apoptosis are induced in this cell population. These results suggest that ER-stress and subsequent apoptosis is induced in healthy photoreceptors, presumably by aberrant accumulation of rhodopsin and the phosphorylation of eukaryotic initiator factor 2α. The explant culture system may allow investigations of neuroprotective strategies.


J Neurosci Methods


Mohlin C,Johansson K




Has Abstract


2011-04-15 00:00:00














  • Setting a gold standard for quantification of leukoaraiosis burden in patients with ischemic stroke: the Atherosclerosis Risk in Communities Study.

    abstract:BACKGROUND:Accurate and reliable measurement of leukoaraiosis, or MR-detected white, matter hyper-intensity (WMH) burden in subjects with acute ischemic stroke (AIS) is important for, ongoing research studies and future models of risk and outcome prediction, but the presence of a, cerebral infarct may complicate measur...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Rost NS,Sadaghiani S,Biffi A,Fitzpatrick KM,Cloonan L,Rosand J,Shibata DK,Mosley TH Jr

    更新日期:2014-01-15 00:00:00

  • A method for the isolation of purified murine neuroepithelial cells from the developing mouse brain.

    abstract::The adult mammalian central nervous system develops from the pseudostratified neuroepithelium of the neural tube. In order to study, in vitro, the differentiation of the neuroepithelial cells in detail and to identify factors that may influence this process, an uncontaminated, viable population of neuroepithelial cell...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Drago J,Murphy M,Bailey KA,Bartlett PF

    更新日期:1991-05-01 00:00:00

  • Selective MCA occlusion: a precise embolic stroke model.

    abstract::The present study describes a method for improving the precision and accuracy of clot placement within the middle cerebral artery (MCA) of rats, utilizing a micro-catheter and laser Doppler flowmetry. This technique reduces the size of clot needed to achieve stable occlusion with no failed embolizations and a low perc...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Dinapoli VA,Rosen CL,Nagamine T,Crocco T

    更新日期:2006-06-30 00:00:00

  • Computed detection and quantitative morphometry of Alzheimer senile plaques.

    abstract::Senile plaques (SP) are the most characteristic neuropathologic lesions of Alzheimer's disease (AD) and studies of plaque cortical distribution, density, and morphology may lead to new information about the origin and pathogenesis of this disease. We have developed an automated computer image analysis program to detec...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hibbard LS,Arnicar-Sulze TL,McKeel DW Jr,Burrell LD

    更新日期:1994-06-01 00:00:00

  • Two variables that can be used as pain indices in experimental animal models of arthritis.

    abstract::Since pain is an important symptom in arthritis, useful behavioral indices for pain in experimental arthritis animal models are important tools for investigative work on arthritis. The purpose of this study was to develop simple and quantifiable behavioral tests, which would represent the level of arthritic pain that ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Yu YC,Koo ST,Kim CH,Lyu Y,Grady JJ,Chung JM

    更新日期:2002-03-30 00:00:00

  • Head movement compensation and multi-modal event detection in eye-tracking data for unconstrained head movements.

    abstract:BACKGROUND:The complexity of analyzing eye-tracking signals increases as eye-trackers become more mobile. The signals from a mobile eye-tracker are recorded in relation to the head coordinate system and when the head and body move, the recorded eye-tracking signal is influenced by these movements, which render the subs...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Larsson L,Schwaller A,Nyström M,Stridh M

    更新日期:2016-12-01 00:00:00

  • Diffuse sleep spindles show similar frequency in central and frontopolar positions.

    abstract::The objective of the present work was to examine fronto-central spindle frequency. A previously validated spindle detector, providing an electroencephalographic (EEG) amplitude independent spindle detection, was used to detect bilateral sleep spindles from sleep EEG recordings of ten healthy subjects with a time resol...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Huupponen E,Kulkas A,Tenhunen M,Saastamoinen A,Hasan J,Himanen SL

    更新日期:2008-07-15 00:00:00

  • Hydraulically coupled microejection technique for precise local solution delivery in tissues.

    abstract::Hydraulically coupled volume microejection (HCVM) was employed to overcome known drawbacks of conventional pressure ejection of drugs in isolated brain slices and other tissues. For HCVM, a solution-filled glass micropipette is connected to a motor-driven, liquid-filled syringe by low-compliance, liquid-filled tubing,...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Pakhomov AG,Semenov I,Brenner R,Toney GM

    更新日期:2006-09-15 00:00:00

  • An ultra-stable non-coherent light source for optical measurements in neuroscience and cell physiology.

    abstract::We demonstrate that high power light-emitting diodes (LED's) exhibit low-frequency noise characteristics that are clearly superior to those of quartz tungsten halogen lamps, the non-coherent light source most commonly employed when freedom from intensity variation is critical. Their extreme stability over tens of seco...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Salzberg BM,Kosterin PV,Muschol M,Obaid AL,Rumyantsev SL,Bilenko Y,Shur MS

    更新日期:2005-01-30 00:00:00

  • An initial validation of the Virtual Environment Grocery Store.

    abstract:BACKGROUND:Virtual reality-based neuropsychological assessments proffer the potential to address the limited ecological validity of pen-and-paper measures of memory. NEW METHOD:To investigate the construct validity of a newly developed virtual reality-based multiple errands task, the Virtual Environment Grocery Store ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Parsons TD,McMahan T

    更新日期:2017-11-01 00:00:00

  • A comparative assessment of gamma-secretase activity in transgenic and non-transgenic rodent brain.

    abstract::Amyloid-beta (Abeta) deposits are one of the hallmarks of the neuropathological degeneration observed in Alzheimer's disease (AD) and Abeta concentrations have been reported to vary in different brain regions of AD patients. Abeta is produced by the sequential cleavage of amyloid precursor protein (APP) by beta-secret...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Goggi JL,Lewis HD,Mok J,Harrison T,Shearman MS,Atack JR,Best JD

    更新日期:2006-10-30 00:00:00

  • Quantitative analysis of acetylcholine in rat brain microdialysates by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.

    abstract::A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-β-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Carrozzo MM,Cannazza G,Pinetti D,Di Viesti V,Battisti U,Braghiroli D,Parenti C,Baraldi M

    更新日期:2010-12-15 00:00:00

  • Detecting overlapped functional clusters in resting state fMRI with Connected Iterative Scan: a graph theory based clustering algorithm.

    abstract::The brain is a complex neural network with interleaving functional connectivity among anatomical regions. However, current functional parcellation algorithms usually emphasize independence or orthogonality between the spatial components, with the interleaving nature underrepresented. This study investigates a method, ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Yan X,Kelley S,Goldberg M,Biswal BB

    更新日期:2011-07-15 00:00:00

  • COLORcation: A new application to phenotype exploratory behavior models of anxiety in mice.

    abstract:BACKGROUND:Behavioral analyses in rodents have successfully delineated the function of many genes and signaling pathways in the brain. Behavioral testing uses highly defined experimental conditions to identify abnormalities in a given mouse strain or genotype. The open field (OF) is widely used to assess both locomotio...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Dagan SY,Tsoory MM,Fainzilber M,Panayotis N

    更新日期:2016-09-01 00:00:00

  • Responses of neurons in the inferior colliculus to binaural disparities: insights from the use of Fisher information and mutual information.

    abstract::The minimal change in a stimulus property that is detectable by neurons has been often quantified using the receiver operating characteristic (ROC) curve, but recent studies introduced the use of the related Fisher information (FI). Whereas ROC analysis and FI quantify the information available for discriminating betw...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Gordon N,Shackleton TM,Palmer AR,Nelken I

    更新日期:2008-04-30 00:00:00

  • A method for calculating current source density (CSD) analysis without resorting to recording sites outside the sampling volume.

    abstract::This article is part of a study on cortical microcircuitry in which flash evoked potentials (FEPs) were recorded from the anesthetized rat visual cortex. The FEPs were subjected to current source density analysis (CSD). One of the limitations of the CSD method is the need for recording sites outside the sampling volum...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Vaknin G,DiScenna PG,Teyler TJ

    更新日期:1988-06-01 00:00:00

  • The utility of functional interaction and cluster analysis in CNS proteomics.

    abstract::Proteomic studies offer enormous potential for gaining insight into cellular dynamics and disease processes. An immediate challenge for enhancing the utility of proteomics in translational research lies in methods of handling and interpreting the large datasets generated. Publications rarely extend beyond lists of pro...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Deighton RF,Short DM,McGregor RJ,Gow AJ,Whittle IR,McCulloch J

    更新日期:2009-06-15 00:00:00

  • Double diffusion encoding and applications for biomedical imaging.

    abstract::Diffusion Magnetic Resonance Imaging (dMRI) is one of the most important contemporary non-invasive modalities for probing tissue structure at the microscopic scale. The majority of dMRI techniques employ standard single diffusion encoding (SDE) measurements, covering different sequence parameter ranges depending on th...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Henriques RN,Palombo M,Jespersen SN,Shemesh N,Lundell H,Ianuş A

    更新日期:2021-01-15 00:00:00

  • A simple, rapid HPLC method for the concurrent measurement of cocaine and catecholamines in brain tissue samples.

    abstract::We describe a simplified HPLC method for the measurement of cocaine and catecholamines in the same brain tissue sample. Using this method to measure cocaine in the brain of rats which received either 10 or 20 mg/kg cocaine injections, we found substantial cocaine concentration differences between medial prefrontal cor...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Carey RJ,DePalma G

    更新日期:1995-05-01 00:00:00

  • The separation and quantification of aminochromes using high-pressure liquid chromatography with electrochemical detection.

    abstract::There is increasing evidence that oxidative stress plays an important role in the pathogenesis of many neurodegenerative diseases including Parkinson's disease (PD). In particular there is support for the participation of oxidized catecholamines in PD. Catecholamines are highly reactive and are readily oxidized to ami...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Ochs SD,Westfall TC,Macarthur H

    更新日期:2005-03-30 00:00:00

  • Realistic simulation of extracellular recordings.

    abstract::In this paper we present an efficient method to generate realistic simulations of extracellular recordings. The method uses a hybrid and computationally simple approach, where the features of the background noise arise naturally from its biophysical process of generation. The generated data resemble the characteristic...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Martinez J,Pedreira C,Ison MJ,Quian Quiroga R

    更新日期:2009-11-15 00:00:00

  • Rectification of the EMG is an unnecessary and inappropriate step in the calculation of Corticomuscular coherence.

    abstract::Corticomuscular coherence (CMC) estimation is a frequency domain method used to detect a linear coupling between rhythmic activity recorded from sensorimotor cortex (EEG or MEG) and the electromyogram (EMG) of active muscles. In motor neuroscience, rectification of the surface EMG is a common pre-processing step prior...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: McClelland VM,Cvetkovic Z,Mills KR

    更新日期:2012-03-30 00:00:00

  • The anterograde neuroanatomical tracer biotinylated dextran-amine: comparison with the tracer Phaseolus vulgaris-leucoagglutinin in preparations for electron microscopy.

    abstract::We investigated the properties of biotinylated dextran-amine (BDA) as a neuroanatomical tracer at the electron microscopic level and we compared the results with those obtained previously with another tracer, the lectin Phaseolus vulgaris-leucoagglutinin (PHA-L). BDA was injected into various brain areas of rats. Foll...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wouterlood FG,Jorritsma-Byham B

    更新日期:1993-06-01 00:00:00

  • Manipulation of vasopressin level in the cerebrospinal fluid of the rat by means of an easily interchangeable controlled-delivery Accurel mini-device.

    abstract::The procedure described for the continuous release of vasopressin (VP) in the rat lateral ventricle via implantation of a VP-loaded Accurel/collodion mini-device has now been further developed, allowing the introduction, removal and exchange of the Accurel device through a polyethylene guide cannula. In this way, mani...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kruisbrink J,Van der Woude TP,Boer GJ,Mirmiran M

    更新日期:1986-08-01 00:00:00

  • A computer program for sequencing and presenting complex sounds for auditory neuroimaging studies.

    abstract::This paper describes a computer program, named ASAP (audio sequencing and presentation), that runs on IBM PC compatible hardware with a Microsoft Windows operating system and has been specially designed for facilitating auditory neuroimaging studies. The program's main characteristics are: (1) the ability to generate ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Kieckhefer E,Kanwal JS

    更新日期:2000-08-15 00:00:00

  • Focused microwave irradiation of the brain preserves in vivo protein phosphorylation: comparison with other methods of sacrifice and analysis of multiple phosphoproteins.

    abstract::At any point in time, net protein phosphorylation represents the contribution of protein kinase and protein phosphatase activities affecting a specific site on a given substrate. Preservation of phosphorylated proteins in neural tissues has traditionally included flash-freezing or fresh tissue processing following tis...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: O'Callaghan JP,Sriram K

    更新日期:2004-05-30 00:00:00

  • An optimized method for enumerating CNS derived memory B cells during viral-induced inflammation.

    abstract:BACKGROUND:CNS inflammation resulting from infection, injury, or neurodegeneration leads to accumulation of diverse B cell subsets. Although antibody secreting cells (ASC) within the inflamed CNS have been extensively examined, memory B cell (Bmem) characterization has been limited as they do not secrete antibody witho...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: DiSano KD,Stohlman SA,Bergmann CC

    更新日期:2017-06-15 00:00:00

  • Directly applied low intensity direct electric current enhances peripheral nerve regeneration in rats.

    abstract::The influence of direct electric stimulation on nerve regeneration was studied in a model of crush injury of the sciatic nerve of rats. Forty-three rats were used and distributed in four groups according to the procedure: (1) intact nerve, inactive circuit; (2) crush injury, inactive circuit; (3) intact nerve, active ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Mendonça AC,Barbieri CH,Mazzer N

    更新日期:2003-10-30 00:00:00

  • A simple and inexpensive device for applying a calibration pulse when performing intracellular recording.

    abstract::A simple and low-cost device is described which facilitates the calibration of intracellular DC recordings in amplitude and time. A current source, optically coupled to a conventional stimulator, injects a pulse of current, across a 1-omega resistor connecting the preparation to ground. Amplitude and duration of the c...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Godfraind JM,Veraart C,Nicaise M

    更新日期:1984-05-01 00:00:00

  • Electrokinetic infusions into hydrogels and brain tissue: Control of direction and magnitude of solute delivery.

    abstract:BACKGROUND:Delivering solutes to a particular region of the brain is currently achieved by iontophoresis for very small volumes and by diffusion from a microdialysis probe for larger volumes. There is a need to deliver solutes to particular areas with more control than is possible with existing methods. NEW METHOD:Ele...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Faraji AH,Jaquins-Gerstl AS,Valenta AC,Weber SG

    更新日期:2019-01-01 00:00:00