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Automated and quantitative image analysis of ischemic dendritic blebbing using in vivo 2-photon microscopy data.

Abstract：

:Ischemia induces a 'blebbing' of dendrites, a structural alteration where dendrites take on a 'beads on a string' appearance. We developed a toolkit program, BlebQuant, for quantitative automated bleb analysis to chart the morphology of dendrites labeled with GFP/YFP under normal conditions and after ischemia-induced damage. In vivo 2-photon data from mouse layer 5 neurons with apical dendritic tufts extending to the cortical surface were examined before, during, and after global ischemia. To quantify changes in dendritic structure, we used morphometric tools that exploit characteristic features of blebbing, distinguished as localized regions of spherical or ellipsoid swellings. By comparing acquired images during ischemia and reperfusion to a pre-ischemia reference image, our automated approach detected blebs based on defined eccentricity and area thresholds and quantified the percentage of blebbed dendrites based on a block-selection method. Our results indicate that the automated morphometric indices we employ yield results that correlate with manual assessment. The automated approach permits rapid and effective analysis of dendritic structure and may facilitate the study of ischemic dendritic damage.

journal_name

J Neurosci Methods

journal_title

Journal of neuroscience methods

authors

Chen S,Tran S,Sigler A,Murphy TH

doi

10.1016/j.jneumeth.2010.12.018

subject

Has Abstract

pub_date

2011-02-15 00:00:00

pages

222-31

issue

2

eissn

0165-0270

issn

1872-678X

pii

S0165-0270(10)00706-5

journal_volume

195

pub_type

杂志文章

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