Abstract：
:Bone marrow stroma cells-derived neural stem cells (BMSCs-D-NSCs) transplantation is a promising strategy for the treatment of nervous system disorders. The development of a non-invasive method to follow the fate of BMSCs-D-NSCs in vivo is very important for the future application of this treatment. In this paper, we show for the first time, that BMSCs-D-NSCs from rhesus monkeys can be labeled in vitro with the superparamagnetic iron oxide (SPIO) contrast agent Feridex and Poly-L-lysine (PLL) without affecting morphology, cell cycle, telomerase activity, proliferation and differentiation ability of the labeled cells. Furthermore, when autografted into the striatum, these cells survived, differentiated and were incorporated into the brain, and could be reliably tracked using MRI, as confirmed by histological examination of the grafting sites with PKH(67) fluorescence. These results suggest that Feridex labeling of BMSCs-D-NSCs is feasible, efficient and safe for MRI tracing following autografting into the rhesus monkey nervous system.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Ke YQ,Hu CC,Jiang XD,Yang ZJ,Zhang HW,Ji HM,Zhou LY,Cai YQ,Qin LS,Xu RXdoi
10.1016/j.jneumeth.2009.01.007subject
Has Abstractpub_date
2009-04-30 00:00:00pages
45-50issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(09)00034-Xjournal_volume
179pub_type
杂志文章abstract：:Unlike certain other brain areas, notably the hippocampal formation, synaptic transmission in the cerebellum, a 3-dimensional anatomical structure, is no ideally studied in a 2-dimensional slice preparation. We describe a method for cutting a cerebellar slice that incorporates two planes of section: one sagittal (in t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00133-6
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abstract：:Mutations in the human prion protein gene (PRNP) are responsible for hereditary diseases called transmissible spongiform encephalopathies (TSE) and a polymorphic site at codon 129 determines sensitivity to infectious forms of these maladies. More recently, codon 129 has been related to cognition performance in the eld...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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abstract：:We describe a simplified HPLC method for the measurement of cocaine and catecholamines in the same brain tissue sample. Using this method to measure cocaine in the brain of rats which received either 10 or 20 mg/kg cocaine injections, we found substantial cocaine concentration differences between medial prefrontal cor...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)00154-9
更新日期：1995-05-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90146-x
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abstract：:An increasing number of neuroscience experiments are using virtual reality to provide a more immersive and less artificial experimental environment. This is particularly useful to navigation and three-dimensional scene perception experiments. Such experiments require accurate real-time tracking of the observer's head ...
journal_title：Journal of neuroscience methods
pub_type： 临床试验,杂志文章
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abstract：BACKGROUND:Whole-cell patch-clamp recording in vivo is the gold-standard method for measuring subthreshold electrophysiology from single cells during behavioural tasks, sensory stimulations, and optogenetic manipulation. However, these recordings require a tight, gigaohm resistance, seal between a glass pipette electro...
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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abstract：:The procedure described for the continuous release of vasopressin (VP) in the rat lateral ventricle via implantation of a VP-loaded Accurel/collodion mini-device has now been further developed, allowing the introduction, removal and exchange of the Accurel device through a polyethylene guide cannula. In this way, mani...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90063-4
更新日期：1986-08-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：2003-03-15 00:00:00
abstract：:The Barnes maze is a visuo-spatial learning and memory test originally designed for use with rats, and later adapted for use with mice. The Barnes maze design and test procedure vary across studies using mice, but the effects of variation in Barnes maze design and test procedure on learning and memory in mice have not...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.09.027
更新日期：2012-01-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/S0165-0270(96)00101-X
更新日期：1996-12-01 00:00:00
abstract：BACKGROUND:Dielectric damage occurring in vivo to neural electrodes, leading to conductive material exposure and impedance reduction over time, limits the functional lifetime and clinical viability of neuroprosthetics. We used silicon micromachined Utah Electrode Arrays (UEAs) with iridium oxide (IrOx) tip metallizatio...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.10.002
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Secondary hyperalgesia is increased sensitivity in normal tissue near an injury, and it is a measure of central sensitization reflecting injury-related effects on the CNS. Secondary hyperalgesia areas (SHAs), usually assessed by polyamide monofilaments, are important outcomes in studies of analgesic drug eff...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,随机对照试验
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.01.009
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.09.013
更新日期：2006-04-15 00:00:00
abstract：:Controlled expression of proteins is a key experimental approach to a deeper understanding of the molecular basis of neuronal function. Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. We demonstrate that HSV-1 amplicon vectors expressing enhanced gree...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00262-5
更新日期：2002-12-15 00:00:00
abstract：:Modern clearing techniques enable high resolution visualization and 3D reconstruction of cell populations and their structural details throughout large biological samples, including intact organs and even entire organisms. In the past decade, these methods have become more tractable and are now being utilized to provi...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)90128-7
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.04.021
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/s0165-0270(98)00062-4
更新日期：1998-08-31 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)90175-9
更新日期：1994-08-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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更新日期：1993-06-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
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abstract：BACKGROUND:While several novel therapeutic approaches for HD are in development, resources to conduct clinical trials are limited. Large animal models have been proposed to improve assessment of safety, tolerability and especially to increase translational reliability of efficacy signals obtained in preclinical studies...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2015.11.013
更新日期：2016-05-30 00:00:00
