Reverse correlation of rapid calcium signals in the zebrafish optic tectum in vivo.


:Reverse correlation techniques provide a quantitative means of computing neuronal input/output relationships. Until now these methods have been limited to electrically recorded responses since unprocessed optical signals generally lack necessary temporal characteristics. We sought to overcome this barrier since combining reverse correlation with calcium imaging would afford a powerful alternative to current methods of measuring response properties of neurons non-invasively in vivo. We labeled zebrafish optic tecta with a calcium indicator and measured responses to a whole-field random flicker light stimulus. Although calcium signals exhibited slow decay kinetics, we could use computational modeling to show that the positive differential of these traces extracts high frequency information. Experimentally, we found that calcium signals processed in this way were synchronous with simultaneously measured synaptic responses and could be used with reverse correlation to determine temporal filters of neurons in the zebrafish optic tectum. These findings demonstrate that calcium responses to physiological stimulation can be processed to obtain rapid signal information and consequently to determine linear filter properties in vivo.


J Neurosci Methods


Ramdya P,Reiter B,Engert F




Has Abstract


2006-10-30 00:00:00














  • Electrokinetic infusions into hydrogels and brain tissue: Control of direction and magnitude of solute delivery.

    abstract:BACKGROUND:Delivering solutes to a particular region of the brain is currently achieved by iontophoresis for very small volumes and by diffusion from a microdialysis probe for larger volumes. There is a need to deliver solutes to particular areas with more control than is possible with existing methods. NEW METHOD:Ele...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Faraji AH,Jaquins-Gerstl AS,Valenta AC,Weber SG

    更新日期:2019-01-01 00:00:00

  • Quantitative assessment of bradykinesia in patients with Parkinson's disease.

    abstract::In order to optimize a method for quantitative assessment of bradykinesia, we evaluated the three-dimensional sources of a movement signal of the wrist and influence of tremor on the reliability of bradykinesia measurements. A total of 33 patients with Parkinson's disease, three patients with Multiple System Atrophy a...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Dunnewold RJ,Jacobi CE,van Hilten JJ

    更新日期:1997-06-06 00:00:00

  • New illumination technique for IR-video guided patch-clamp recording from neurons in slice cultures on biomembrane.

    abstract::Slice cultures on biomembrane are the method of choice for studying Ca2+-dependent plastic changes occurring over several days to weeks. Using IR-differential interference contrast, good visualization of neurons in biomembrane slice cultures has been achieved despite a negative optical effect of the biomembrane, but e...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Alix P,Winterer J,Müller W

    更新日期:2003-09-30 00:00:00

  • Silver staining method for demonstrating Lewy bodies in Parkinson's disease and argyrophilic oligodendrocytes in multiple system atrophy.

    abstract::A reliable and economical silver staining method is recommended for demonstration of Lewy bodies (LB) and Lewy neurites (LN) in Parkinson's disease (PD) and of argyrophilic oligodendrocytes in multiple system atrophy (MSA). The technique can be applied to routinely formalin-fixed autopsy material and does not require ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Braak E,Braak H

    更新日期:1999-02-01 00:00:00

  • Neurons on Parafilm: versatile elastic substrates for neuronal cell cultures.

    abstract::A variety of materials has been applied to neuronal cell culture substrates to improve the efficiency of the culture and to provide pertinent cell growth environment. Here we report the application of Parafilm(®) M ('Parafilm') as a novel substrate for neuronal culture and patterning. Cell culture results show that el...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Yoo SJ,Nam Y

    更新日期:2012-02-15 00:00:00

  • Response of zebrafish larvae to mild electrical stimuli: A 96-well setup for behavioural screening.

    abstract:BACKGROUND:Zebrafish larvae have a high potential as model system to replace rodents, especially in screening and drug discovery applications. However, an experimental setup to deliver mild electrical stimuli with simultaneous high throughput behavioural tracking has not yet been described. NEW METHOD:A new tool was d...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Steenbergen PJ

    更新日期:2018-05-01 00:00:00

  • Recording electrophysiological data on video tape: a superior and less costly alternative to conventional tape recorders.

    abstract::Electrical potentials recorded extracellularly from the sinus gland of the isopod, Oniscus asellus, were stored on video tape with the aid of a digital-audio (DA) processor and a video cassette recorder (VCR). The DA processor transforms the analog signal to digital pulses of equal amplitude and converts these pulses ...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Chiang RG,Knobloch CA,Singleton DM,Steel CG,Davey KG

    更新日期:1985-10-01 00:00:00

  • Flavopiridol reduces the impedance of neural prostheses in vivo without affecting recording quality.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Purcell EK,Thompson DE,Ludwig KA,Kipke DR

    更新日期:2009-10-15 00:00:00

  • Efficient gene transfers into neocortical neurons connected by NMDA NR1-containing synapses.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Nagayach A,Singh A,Geller AI

    更新日期:2019-11-01 00:00:00

  • Inter-rater and intra-rater reliability of cerebral blood flow measures obtained using the Brain Acoustic Monitor.

    abstract:BACKGROUND:Current assessment of sport-related concussion involves a multifaceted approach including neurocognitive testing, symptom report, and balance assessment. Lacking from this paradigm is a truly objective measure of the physiological changes associated with concussion. NEW METHOD:A device called the Brain Acou...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Acocello S,Broshek DK,Saliba S

    更新日期:2014-01-15 00:00:00

  • Overexpression of growth-associated proteins in the neurons of adult transgenic mice.

    abstract::The Thy-1.2 expression cassette described in this report drives strong constitutive expression of transgenes specifically in the neurons of adult transgenic mice. Transgene expression begins at P6-10, at a time when it can affect activity-dependent rearrangements of synaptic connections and neuron-glia interactions du...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Caroni P

    更新日期:1997-01-01 00:00:00

  • A symmetrical Waxholm canonical mouse brain for NeuroMaps.

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    pub_type: 杂志文章


    authors: Bowden DM,Johnson GA,Zaborsky L,Green WD,Moore E,Badea A,Dubach MF,Bookstein FL

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  • A dual RF resonator system for high-field functional magnetic resonance imaging of small animals.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Ludwig R,Bodgdanov G,King J,Allard A,Ferris CF

    更新日期:2004-01-30 00:00:00

  • Human stem cells isolated from adult skeletal muscle differentiate into neural phenotypes.

    abstract::Multipotent neural stem cells have been isolated from the adult [Kirschenbaum B, Nedergaard M, Preuss A, Barami K, Fraser RA, Goldman SA. In vitro neuronal production and differentiation by precursor cells derived from the adult human forebrain. Cereb Cortex 1994;4(6):576-89; Laywell ED, Kukekov VG, Steindler DA. Mult...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Schultz SS,Lucas PA

    更新日期:2006-04-15 00:00:00

  • Spike sorting based on discrete wavelet transform coefficients.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Letelier JC,Weber PP

    更新日期:2000-09-15 00:00:00

  • Characterization of the temporo-spatial effects of chronic bilateral intrahippocampal cannulae on interleukin-1beta.

    abstract::The implantation of a foreign object in the brain produces an acute neuroinflammatory state in which glia (astrocytes and microglia) may remain chronically activated in response to the inert foreign object. Activated glia can exhibit a sensitized pro-inflammatory response to immunogenic stimuli. This may be relevant t...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Holguin A,Frank MG,Biedenkapp JC,Nelson K,Lippert D,Watkins LR,Rudy JW,Maier SF

    更新日期:2007-04-15 00:00:00

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hiscock JJ,Murphy S,Willoughby JO

    更新日期:2000-01-31 00:00:00

  • Stimulation of Ca²+ signals in neurons by electrically coupled electrolyte-oxide-semiconductor capacitors.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Giacomello M,Girardi S,Scorzeto M,Peruffo A,Maschietto M,Cozzi B,Vassanelli S

    更新日期:2011-05-15 00:00:00

  • Cerebellar neurotransmission in attention-deficit/hyperactivity disorder: does dopamine neurotransmission occur in the cerebellar vermis?

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Glaser PE,Surgener SP,Grondin R,Gash CR,Palmer M,Castellanos FX,Gerhardt GA

    更新日期:2006-02-15 00:00:00

  • Transfection of rat brain cells by electroporation.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Li H,Chan ST,Tang F

    更新日期:1997-07-18 00:00:00

  • Lacking quality in research: Is behavioral neuroscience affected more than other areas of biomedical science?

    abstract::There are many reasons why novel therapeutics fail in clinical trials but these failures are often attributed to lacking quality of preclinical data. These problems are not limited to any specific therapeutic area, academic or industrial research and are due in large part to several generic factors influencing researc...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Bespalov A,Steckler T

    更新日期:2018-04-15 00:00:00

  • Microwave-stimulated recovery of myosin-V immunoreactivity from formalin-fixed, paraffin-embedded human CNS.

    abstract::The lability of brain myosin-V (BM-V) to aldehyde-fixation has hindered immunohistochemical (IH) studies of this actin-based motor. We show here that BM-V immunoreactivity (IR) can be retrieved from formalin-fixed, paraffin-embedded human tissue. BM-V IR was optimally retrieved by boiling 5 microm cerebellar tissue se...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Martins AR,Dias MM,Vasconcelos TM,Caldo H,Costa MC,Chimelli L,Larson RE

    更新日期:1999-10-15 00:00:00

  • Pressure injections of fluid in the nanoliter range via micropipettes.

    abstract::The relationship between pressure, ejection duration and volume ejected was experimentally determined in vitro for micropipettes with different external tip diameters. The relationship between ejection duration and ejected volume is linear in the steady state (i.e. with ejection durations of 1 s or longer) and at suff...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: van Dongen PA

    更新日期:1984-04-01 00:00:00

  • Recovering Wood and McCarthy's ERP-prototypes by means of ERP-specific procrustes-rotation.

    abstract:BACKGROUND:The misallocation of treatment-variance on the wrong component has been discussed in the context of temporal principal component analysis of event-related potentials. There is, until now, no rotation-method that can perfectly recover Wood and McCarthy's prototypes without making use of additional information...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Beauducel A

    更新日期:2018-02-01 00:00:00

  • On the use of ibotenic acid to lesion selectively different components of the hippocampal formation.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Jarrard LE

    更新日期:1989-09-01 00:00:00

  • Synaptic release of zinc from brain slices: factors governing release, imaging, and accurate calculation of concentration.

    abstract::Cerebrocortical neurons that store and release zinc synaptically are widely recognized as critical in maintenance of cortical excitability and in certain forms of brain injury and disease. Through the last 20 years, this synaptic release has been observed directly or indirectly and reported in more than a score of pub...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Frederickson CJ,Giblin LJ 3rd,Balaji RV,Masalha R,Frederickson CJ,Zeng Y,Lopez EV,Koh JY,Chorin U,Besser L,Hershfinkel M,Li Y,Thompson RB,Krezel A

    更新日期:2006-06-30 00:00:00

  • The M-Maze task: An automated method for studying fear memory in rats exposed to protracted aversive conditioning.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Souza RR,Robertson NM,Pruitt DT,Noble L,Meyers EC,Gonzales PA,Bleker NP,Carey HL,Hays SA,Kilgard MP,McIntyre CK,Rennaker RL

    更新日期:2018-03-15 00:00:00

  • A microassay for simultaneous measurement of in vivo rates of tryptophan hydroxylation and levels of serotonin in discrete brain nuclei.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Long JB,Youngblood WW,Kizer JS

    更新日期:1982-07-01 00:00:00

  • Novel model of orthotopic U-87 MG glioblastoma resection in athymic nude mice.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Bianco J,Bastiancich C,Joudiou N,Gallez B,des Rieux A,Danhier F

    更新日期:2017-06-01 00:00:00

  • An improved method for protecting and fixing the lumbar catheters placed in the spinal subarachnoid space of rats.

    abstract::The chronic intrathecal catheterization of the subarachnoid space in the rat is a very useful model in the research of neuroscience, anesthesiology, neurotoxicity, and etc. To avoid high postoperative mortality and neurological impairment, the catheterization from the lumbar interspace has been widely used in recent y...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Xu F,Li T,Zhang B

    更新日期:2009-10-15 00:00:00