Abstract：
:A simple method which allows easy quantification of neuronal and glial sub-populations in primary neuronal tissue culture is described. The method takes advantage of the fact that the staining of neuronal and glia nuclei with Hoechst 33258 is distinctive and characteristic of the cell type. Double-staining experiments show that only cells with nuclear staining characteristic of neurones are immunopositive for neuronal markers. There is no obvious difference between neurones from different regions of the brain nor any apparent differences in nuclear staining of neurones of different sizes. This method is particularly useful for the quantification of neurones in cultures where neurones are growing on glial beds or where the neurones are growing in clusters. Further, it eliminates the need to double-stain cultures with neuronal markers in order to calculate neuronal sub-populations. We have used this technique to examine the effect of time in vitro on the proportion of calbindin D28K-positive neurones in striatal cultures. We show that with increasing time in culture, there is a significant increase in the percentage of neurones which express calbindin D28K. This supports the suggestion that calbindin D28K may be important for promoting the survival of neurones in which it is expressed.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Tucker LM,Morton AJdoi
10.1016/0165-0270(94)00207-wsubject
Has Abstractpub_date
1995-07-01 00:00:00pages
217-23issue
2eissn
0165-0270issn
1872-678Xpii
016502709400207Wjournal_volume
59pub_type
杂志文章abstract：:The autoregressive (AR) model is widely used in electroencephalogram (EEG) analyses such as waveform fitting, spectrum estimation, and system identification. In real applications, EEGs are inevitably contaminated with unexpected outlier artifacts, and this must be overcome. However, most of the current AR models are b...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.11.007
更新日期：2015-01-30 00:00:00
abstract：:A novel version of the 8-arm radial maze task was developed to quantify spatial learning and memory in rats subjected to transient cerebral ischemia (TCI) using the 4-VO model. This maze uses the rat's natural behavior of avoiding open, illuminated areas, and preference for a darkened, enclosed shelter. Ischemic rats ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2003.08.012
更新日期：2004-01-15 00:00:00
abstract：:We present a numerical method which provides the ability to analyze digitized microscope images of retinal explants and quantify neurite outgrowth. Few parameters are required as input and limited user interaction is necessary to process an entire experiment of images. This eliminates fatigue related errors and user-r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00017-7
更新日期：2003-04-15 00:00:00
abstract：:Combining multiple linear univariate features in one feature space and classifying the feature space using machine learning methods could predict epileptic seizures in patients suffering from refractory epilepsy. For each patient, a set of twenty-two linear univariate features were extracted from 6 electroencephalogra...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.03.019
更新日期：2013-07-15 00:00:00
abstract：:Experiments based on neuronal cell-transistor couplings were made from some groups during the last years. Pioneering work in this field was carried out by Fromherz and his group (Fromherz, 2003; Schmidtner and Fromherz, 2006). We were interested of the interaction of nerve cells to serine hydrolase inhibitor diisoprop...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.02.018
更新日期：2012-01-01 00:00:00
abstract：:The 2-deoxyglucose (2DG) histochemical method of Sokoloff et al. ((1977) J. Neurochem., 28: 897-916) has been widely used to map metabolic activity in the brain, but lacks sufficient resolution to view individual neurons. Modifications of the original method have achieved cellular resolution with emulsion autoradiogra...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(96)00063-5
更新日期：1996-09-01 00:00:00
abstract：:Quantifying the connectivity between arbitrary surface patches in the human brain cortex can be used in studies on brain function and to characterize clinical diseases involving abnormal connectivity. Cortical regions of human brain in their natural forms can be represented in surface formats. In this paper, we presen...
journal_title：Journal of neuroscience methods
pub_type： 临床试验,杂志文章
doi：10.1016/j.jneumeth.2011.02.017
更新日期：2011-04-30 00:00:00
abstract：BACKGROUND:The brain generates signals in a wide frequency range (∼2840 Hz). Existing magnetoencephalography (MEG) methods typically detect brain activity in a median-frequency range (1-70 Hz). The objective of the present study was to develop a new method to utilize the frequency signatures for source imaging. NEW ME...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.10.007
更新日期：2015-01-15 00:00:00
abstract：:After injection of 2-deoxyglucose (2-DG) labeled with tritium or carbon-14, autoradiograms were produced by thaw-mounting 4 micron frozen sections of rat brains on nuclear emulsion-coated slides. The results show that the distribution of radioactivity among different brain regions was similar and that the resolution a...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(87)90043-4
更新日期：1987-06-01 00:00:00
abstract：:The adult mammalian central nervous system develops from the pseudostratified neuroepithelium of the neural tube. In order to study, in vitro, the differentiation of the neuroepithelial cells in detail and to identify factors that may influence this process, an uncontaminated, viable population of neuroepithelial cell...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90031-t
更新日期：1991-05-01 00:00:00
abstract：:Intra-cranial electroencephalographic recordings are increasingly employed in mice because of the availability of genetically manipulated mouse models. Currently, dental acrylic and anchoring screws are used to cement implanted electrodes. This technique works well for adult animals but often encounters difficulty whe...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.03.001
更新日期：2008-06-15 00:00:00
abstract：:We present an efficient algorithm for solving the one-dimensional cable equation in the Laplace (frequency) domain for an arbitrary linear membrane. This method, a reformulation and extension of the geometrical calculus developed by Butz and Cowan (1974), solves for the transfer impedance between any two points in a b...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90015-9
更新日期：1985-02-01 00:00:00
abstract：:The reproducibility of motor unit twitches obtained using spike-triggered averaging (STA) was examined in the human first dorsal interosseus. For each motor unit (30 total) a series of STA twitches was derived using a 30 s averaging window. Within each averaging window, eight independent measures characterizing motor ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00077-3
更新日期：2003-06-30 00:00:00
abstract：:We have built a high speed, sensitive camera system capable of capturing sequences of low-light level images synchronized with recordings of membrane potential. The camera system is based on a cooled, scientific grade CCD camera controlled by a PC/AT computer. It can take 100 frames/sec of 18 X 18 element images and 4...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90051-z
更新日期：1991-02-01 00:00:00
abstract：:We have attempted to demonstrate the technical requirements and performance of a microscopical assay using a densitometric application of image analysis to measure immunohistochemical stain intensity. Not surprisingly, the techniques required were more demanding than those used for the quantification of field and obje...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90048-i
更新日期：1992-10-01 00:00:00
abstract：:Functionally relevant large scale brain dynamics operates within the framework imposed by anatomical connectivity and time delays due to finite transmission speeds. To gain insight on the reliability and comparability of large scale brain network simulations, we investigate the effects of variations in the anatomical ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2009.07.007
更新日期：2009-09-30 00:00:00
abstract：BACKGROUND:Secondary hyperalgesia is increased sensitivity in normal tissue near an injury, and it is a measure of central sensitization reflecting injury-related effects on the CNS. Secondary hyperalgesia areas (SHAs), usually assessed by polyamide monofilaments, are important outcomes in studies of analgesic drug eff...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jneumeth.2015.08.018
更新日期：2015-12-30 00:00:00
abstract：BACKGROUND:Intracerebral hemorrhage (ICH) is a stroke subtype with the highest mortality rate. Hematoma expansion and re-bleeding post-ICH are common and exacerbate the initial cerebral insult. There is a need for continuous monitoring of the neurologic status of patients with an ICH injury. NEW METHOD:A prototype dev...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.05.025
更新日期：2014-09-30 00:00:00
abstract：:Optical imaging over extended periods of time in non-human primates presents serious challenges because the dura mater must be removed to expose the cortical surface. We present a novel nylon imaging chamber with a transparent artificial dura implant, which allows repeated, long-term optical recordings from the cortex...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00475-7
更新日期：2002-01-15 00:00:00
abstract：:A reliable and economical silver staining method is recommended for demonstration of Lewy bodies (LB) and Lewy neurites (LN) in Parkinson's disease (PD) and of argyrophilic oligodendrocytes in multiple system atrophy (MSA). The technique can be applied to routinely formalin-fixed autopsy material and does not require ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(98)00173-3
更新日期：1999-02-01 00:00:00
abstract：:A method for the reconstruction of the individual spike trains from extracellular multineuron recordings is described. A neural network emulation program is trained to recognize a sample set of digitized spikes. The digitized spikes are fed into the neural network, and the network output is used to classify spikes in ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90125-y
更新日期：1990-12-01 00:00:00
abstract：BACKGROUND:Artificial neural networks (ANNs) are one of the widely used classifiers in the brain-computer interface (BCI) systems-based on noninvasive electroencephalography (EEG) signals. Among the different ANN architectures, the most commonly applied for BCI classifiers is the multilayer perceptron (MLP). When appro...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.05.001
更新日期：2018-07-15 00:00:00
abstract：:Synthesis of melatonin in the mammalian pineal gland is regulated by the rhythmic expression of acetyl-CoA: serotonin N-acetyltransferase (SNAT) and other unknown factors. To screen for pineal-specific mRNAs potentially involved in melatonin synthesis and/or regulation, differential display PCR (DDPCR) was employed. W...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)02237-1
更新日期：1997-05-16 00:00:00
abstract：BACKGROUND:Although they form a unitary phenomenon, the relationship between extracranial M/EEG and transmembrane ion flows is understood only as a general principle rather than as a well-articulated and quantified causal chain. METHOD:We present an integrated multiscale model, consisting of a neural simulation of tha...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.10.002
更新日期：2019-03-15 00:00:00
abstract：BACKGROUND:The myelin sheath produced by glial cells insulates the axons, and supports the function of the nervous system. Myelin sheath degeneration causes neurodegenerative disorders, such as multiple sclerosis (MS). There are no therapies for MS that promote remyelination. Drug discovery frequently involves screenin...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108946
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Sensory suppression is an important brain function for appropriate processing of information and is known to be impaired in patients with various types of mental illness. Long latency suppression which is a paradigm using change-related cortical response with repeated paired pulses embedded in a train of con...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2021.109087
更新日期：2021-01-25 00:00:00
abstract：:Immunohistochemistry using antiserum against the basement membrane glycoprotein laminin, was shown to be an excellent marker for brain blood vessels. Throughout the brain of mice, rats, guinea pigs, monkeys and humans, the basement membrane of the vascular structures were strongly laminin-positive. The neuropil itself...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90128-7
更新日期：1986-09-01 00:00:00
abstract：:The ability to detect and sort overlapping spike waveforms in extracellular recordings is key to studies of neural coding at high spatial and temporal resolution. Most spike-sorting algorithms are based on initial spike detection (e.g. by a voltage threshold) and subsequent waveform classification. Much effort has bee...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2008.06.011
更新日期：2008-09-15 00:00:00
abstract：:Neural systems continuously optimize how organisms process their environment and are highly dynamic. Building predictive models of these systems is challenging due to the large number of their elements. Therefore, in experimental and descriptive neurobiology the researcher typically does not seek to catalogue all vari...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.02.012
更新日期：2012-01-01 00:00:00
abstract：:Threshold depolarization is defined as the amplitude of the depolarization that will just bring a neurone to its firing threshold. Here, a novel technique is described for the measurement of threshold depolarization in resting human motoneurones. The tibial nerve was stimulated at an intensity that was just sufficient...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90097-j
更新日期：1991-08-01 00:00:00
