Muscarinic receptor family interacting proteins: role in receptor function.


:G protein-coupled receptors constitute one of the most important families of membrane receptors through which cells respond to extracellular stimuli. Receptors of this superfamily likely function as signal transduction complexes. The identification and analysis of their components provide new insights into a better understanding of these receptors' function and regulation. We used tandem-affinity purification and mass spectrometry as a systematic approach to characterize multiprotein complexes in the acetylcholine muscarinic receptor subfamily. To overcome the limitations associated with membrane protein receptor solubilization with detergents, we developed a strategy in which receptors are co-expressed with a cytoplasmic minigene construct, encoding the third intracellular loop and the C-terminal tail tagged to the tandem-affinity-cassette of each receptor subtype. Numerous protein complexes were identified, including many new interactions in various signalling pathways. Systematic identification data set together with protein interactions reported in the literature revealed a high degree of connectivity. These allow the proposal, for the first time, of an outline of the muscarinic interactome as a network of protein complexes and a context for a more reasoned and informed approach to drug discovery and muscarinic receptor subtype specificities.


J Neurosci Methods


Borroto-Escuela DO,Correia PA,Romero-Fernandez W,Narvaez M,Fuxe K,Ciruela F,Garriga P




Has Abstract


2011-02-15 00:00:00














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    abstract::We investigated the properties of biotinylated dextran-amine (BDA) as a neuroanatomical tracer at the electron microscopic level and we compared the results with those obtained previously with another tracer, the lectin Phaseolus vulgaris-leucoagglutinin (PHA-L). BDA was injected into various brain areas of rats. Foll...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Wouterlood FG,Jorritsma-Byham B

    更新日期:1993-06-01 00:00:00

  • A surgical technique of spinal cord cell transplantation in amyotrophic lateral sclerosis.

    abstract::We report an original method for implanting bone marrow stem cells within the spinal cord parenchyma. This method was used for the experimental treatment of patients diagnosed with amyotrophic lateral sclerosis. The methodology is reproducible and devoid of major complications even in patients showing significant spin...

    journal_title:Journal of neuroscience methods

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    authors: Blanquer M,Pérez-Espejo MA,Martínez-Lage JF,Iniesta F,Martinez S,Moraleda JM

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    abstract::Chimera construction between different proteins is a useful method for investigating protein structure and function relationships. However, this technique, by its traditional application, has been daunting because of its complex procedure and low success rate. Here we describe a protocol for constructing chimeras betw...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Shan Q,Lynch JW

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Nam Y,Brown EA,Ross JD,Blum RA,Wheeler BC,DeWeerth SP

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Long JB,Youngblood WW,Kizer JS

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  • A wavelet based algorithm for the identification of oscillatory event-related potential components.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Aniyan AK,Philip NS,Samar VJ,Desjardins JA,Segalowitz SJ

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  • SWDreader: a wavelet-based algorithm using spectral phase to characterize spike-wave morphological variation in genetic models of absence epilepsy.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Richard CD,Tanenbaum A,Audit B,Arneodo A,Khalil A,Frankel WN

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Lennard PR,Boss V,Shirley KA

    更新日期:1989-08-01 00:00:00

  • A flow rate monitor for a variety of physiological preparations, including in vitro brain slices.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Rinaldi PC,Brown AM

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  • Experimental microneurosurgery of the trigeminal nerve: surgical technique for ganglionectomy and rhizotomy in the cat.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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  • Confocal microscopic estimation of GABAergic nerve terminals in the central nervous system.

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Hiscock JJ,Murphy S,Willoughby JO

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Davey CE,Soto-Breceda A,Shafton A,McAllen RM,Furness JB,Grayden DB,Stebbing MJ

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Lee ST,Park JE,Lee K,Kang L,Chu K,Kim SU,Kim M,Roh JK

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Qureshi F,Yan J

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    pub_type: 杂志文章


    authors: Furdea A,Ruf CA,Halder S,De Massari D,Bogdan M,Rosenstiel W,Matuz T,Birbaumer N

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Worley A,Fabrizi L,Boyd S,Slater R

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Olsen OH,Murray-Smith D

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    journal_title:Journal of neuroscience methods

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


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    abstract::The GABA analogue baclofen [3-(p-chlorophenyl)-4-aminobutanoic acid] has stereospecific actions on the peripheral and central nervous systems. This paper describes the resolution of tritium-labelled baclofen by high performance liquid chromatography on a reverse-phase C18 column using a chiral mobile phase. The method...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Weatherby RP,Allan RD,Johnston GA

    更新日期:1984-01-01 00:00:00

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    abstract::This study describes a modified Hargreaves' method for assessing paw withdrawal threshold temperatures for heat (PWT-H) nociception in the hind paws of rats. This method utilises radiant heat to maintain controlled lamp temperatures (CLTs) on a glass floor beneath the rat hind paw. An ascending series of CLTs were app...

    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Banik RK,Kabadi RA

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Lomber SG

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Salvatore C,Cerasa A,Castiglioni I,Gallivanone F,Augimeri A,Lopez M,Arabia G,Morelli M,Gilardi MC,Quattrone A

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章,评审


    authors: Pisano F,Pisanello M,De Vittorio M,Pisanello F

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Çimen Yetiş S,Çapar A,Ekinci DA,Ayten UE,Kerman BE,Töreyin BU

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    journal_title:Journal of neuroscience methods

    pub_type: 杂志文章


    authors: Lisiecki RS,Voigt HF

    更新日期:1995-08-01 00:00:00