Abstract：
:A variety of materials has been applied to neuronal cell culture substrates to improve the efficiency of the culture and to provide pertinent cell growth environment. Here we report the application of Parafilm(®) M ('Parafilm') as a novel substrate for neuronal culture and patterning. Cell culture results show that elastic Parafilm had effects on cell viability, length and number of neurites, and soma spreading. Parafilm was also an effective substrate to obtain patterned neuronal cultures using a conventional micro-contract printing (μCP) technique. Polylysine micropatterns in line or grid forms were readily transferred from PDMS stamp to bare Parafilm surfaces and spatially confined neuronal cultures were successfully maintained for over three weeks. We also demonstrate that batch-processing cell culture substrates can be easily fabricated using a piece of Parafilm. The softness, plasticity, and hydrophobicity were main features that made it attractive for Parafilm to be considered as a practical cell culture platform. The results can be extended to develop an inexpensive and practical neuronal culture substrates in tissue engineering and biochip applications.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Yoo SJ,Nam Ydoi
10.1016/j.jneumeth.2011.10.023subject
Has Abstractpub_date
2012-02-15 00:00:00pages
28-34issue
1eissn
0165-0270issn
1872-678Xpii
S0165-0270(11)00623-6journal_volume
204pub_type
杂志文章abstract：:Vertex analysis defines dichotomous networks in terms of the relative frequencies of pendant (Vp) and nodal (Vd) vertices. Va, Vb and Vc comprise the 3 classes of Vd which connect 2, 1 and 0 Vp respectively. The vertex ratio (VR) of Va/Vb may be used to define topology since Va and Vc approach equality in large networ...
journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2006.06.004
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2005.02.009
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journal_title：Journal of neuroscience methods
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.10.020
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2013.11.027
更新日期：2014-02-15 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00142-5
更新日期：2002-08-30 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(91)90031-t
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108988
更新日期：2020-11-04 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(86)90128-7
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.12.004
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doi：10.1016/s0165-0270(00)00255-7
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2009.04.012
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doi：10.1016/s0165-0270(97)00114-3
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2014.08.022
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.10.002
更新日期：2018-01-01 00:00:00
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.06.013
更新日期：2017-08-15 00:00:00
abstract：:The whole cell patch clamp technique allows recording of membrane currents in an entire cell under voltage clamp conditions. However, technical difficulties arise in large cells bearing extensive processes, such as human fetal dorsal root ganglion (DRG) neurons in culture. In order to improve space clamp conditions, h...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90094-v
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journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.08.037
更新日期：2012-01-30 00:00:00
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journal_title：Journal of neuroscience methods
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doi：10.1016/j.jneumeth.2004.02.024
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abstract：:MicroRNAs (miRNAs) finely tune messenger RNA (mRNA) expression. As the brain is a highly heterogeneous tissue, physiologically relevant miRNA expression profiling greatly benefits from sampling brain regions or nuclei. MiRNA expression profiling from individual samples is also important for investigating potential dif...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.05.033
更新日期：2012-07-30 00:00:00
