Abstract:
:Biolistics, also known as particle-mediated gene transfer, has been used as an effective, method to transfect primary neurons in cultured slices when all other methods have proven unsuccessful. Most of these uses have provided qualitative or semi-quantitative data based on visual assays such as immunohistochemistry. In this paper, we describe a quantitative method of biolistics to analyze gene expression in organotypic cultures of hippocampus and hypothalamus. The method involves co-transfection of the experimental promoters and standard (cytomegalovirus or Rous sarcoma virus) promoters coupled to different reporters (luciferase or beta-galactosidase), with the standard promoter-reporter construct used to 'normalize' the experimental data. Examples and validations of this technique with various cell specific promoters are given: for example, astrocyte-specific and neuron-specific (alpha-tubulin and N-type calcium channel alpha-1B gene) promoters and various tissues (Neuro 2A cells and hippocampal and hypothalamic organotypic slice-explants). An analysis of deletion constructs of the alpha 1B calcium channel subunit gene is described. This method should provide a new opportunity for the analysis of gene expression in diverse neuronal phenotypes.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Thomas A,Kim DS,Fields RL,Chin H,Gainer Hdoi
10.1016/s0165-0270(98)00117-4subject
Has Abstractpub_date
1998-10-01 00:00:00pages
181-91issue
1-2eissn
0165-0270issn
1872-678Xpii
S0165-0270(98)00117-4journal_volume
84pub_type
杂志文章abstract:BACKGROUND:The widespread use of data-driven methods, such as independent component analysis (ICA), for the analysis of functional magnetic resonance imaging data (fMRI) has enabled deeper understanding of neural function. However, most popular ICA algorithms for fMRI analysis make several simplifying assumptions, thus...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2016.03.012
更新日期:2016-05-01 00:00:00
abstract::Many behavioural tests in rodents are based on the premise that basal locomotor activity of the animals is similar between the tested groups. The measurement of basal home cage activity is therefore an essential parameter, that should be included in all studies which employ tests of anxiety or cognition. Currently ava...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2007.01.008
更新日期:2007-05-15 00:00:00
abstract::This paper describes a technique that has been developed to assess the in vivo morphology of central nervous system (CNS) tissue by immunofluorescence. This technique permits the study of tissue that is mainly just a monolayer of cells. Unlike routine cryosections that are much thicker (10-15 microm), imprinting does ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(00)00237-5
更新日期:2000-07-31 00:00:00
abstract::The permanence of retrograde neuronal labeling by the fluorescent tracers Fast Blue, Fluoro-Gold, Mini-Ruby, Fluoro-Ruby and Fluoro-Emerald was investigated in adult rat spinal motorneurons at 1, 4, 12 and 24 weeks after tracer application to a transected muscle nerve. After 1 week, the largest number of retrogradely ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)02227-9
更新日期:1997-06-06 00:00:00
abstract::We have designed a device for long-term head fixation for use in behaving nonhuman primates that is robust yet minimally invasive and simple to use. This device is a modified version of the halo system that is used in humans for cervical traction and stabilization after spinal column injuries. This device consists of ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.04.012
更新日期:2009-06-30 00:00:00
abstract::We have developed a quantitative reverse transcription polymerase chain reaction (RT-PCR) method, using both internal and external standardisation, to quantitate fg amounts of pre-pro-thyrotropin releasing hormone (pre-pro-TRH) mRNA in the paraventricular nucleus (PVN) of individual laboratory rats. A constant amount ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(96)00090-8
更新日期:1996-10-01 00:00:00
abstract:BACKGROUND:Flow cytometry is an efficient and powerful technique to characterize and quantify numerous cells. However, the strengths of this technique have not been widely harnessed in neurosciences due to the critical step of CNS tissue preparation into a single cell suspension. Previous reports assessed either neural...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2015.03.021
更新日期:2015-05-30 00:00:00
abstract::A current source for neural stimulation is presented which converts arbitrary voltage signals to current-controlled signals while regulating the offset-voltage across the stimulation electrodes in order to keep the electrodes in an electrochemical state that allows for injecting a maximum charge. The offset-voltage ca...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.03.016
更新日期:2008-06-30 00:00:00
abstract::Intercellular calcium waves in central nervous system astrocyte networks underline the principle mechanism of cell signaling in astrocyte syncsytiums, which putatively contribute to the modulation of neuronal signaling and metabolic regulation. In support of carrying out systems level analyses of astrocyte networks, w...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.01.013
更新日期:2008-05-30 00:00:00
abstract::Transcranial Electrical Stimulation (tES) encompasses all methods of non-invasive current application to the brain used in research and clinical practice. We present the first comprehensive and technical review, explaining the evolution of tES in both terminology and dosage over the past 100 years of research to prese...
journal_title:Journal of neuroscience methods
pub_type: 历史文章,杂志文章,评审
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更新日期:2013-10-15 00:00:00
abstract::We describe a novel procedure for combining immunocytochemistry with in situ hybridisation. In contrast to previously published procedures, the technique involves immunofluorescence followed by in situ hybridization and is particularly suitable for antigens which are labile or sensitive to in situ hybridization proces...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(05)80011-1
更新日期:1993-06-01 00:00:00
abstract:BACKGROUND:Neurotrauma patients face major neurological sequelae. The failure in the preclinical-to-clinical translation of candidate therapies could be due to poor evaluation of rodent behaviours after neurotrauma. NEW METHOD:A home cage automated system was used to study the long term behaviour of individual rats wi...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.04.005
更新日期:2019-06-01 00:00:00
abstract:BACKGROUND:Single unit recording in behaving nonhuman primates is widely used to study the primate central nervous system. However, certain questions cannot be addressed without recording large numbers of neurons simultaneously. Multiple 96-electrode probes can be implanted at one time, but certain problems must be ove...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2017.07.001
更新日期:2017-09-01 00:00:00
abstract:INTRODUCTION:Transcranial magnetic stimulation (TMS) is used for assessing the excitability of cortical neurons and corticospinal pathways by determining the subject-specific motor threshold (MT). However, the MT is dependent on the TMS instrumentation and exhibits large variation. We hypothesized that between-subject ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.10.004
更新日期:2012-01-30 00:00:00
abstract::Benign schwannomas are common tumors of the cranial and peripheral nerves, causing pain and loss of function. The development of effective therapy for these tumors has been hampered by the lack of relevant experimental in vivo models for convenient testing. Here, we describe a novel schwannoma model in which an immort...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2010.10.021
更新日期:2011-01-30 00:00:00
abstract::We present an efficient algorithm for solving the one-dimensional cable equation in the Laplace (frequency) domain for an arbitrary linear membrane. This method, a reformulation and extension of the geometrical calculus developed by Butz and Cowan (1974), solves for the transfer impedance between any two points in a b...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(85)90015-9
更新日期:1985-02-01 00:00:00
abstract::We present a simple and efficient method for expressing cDNAs in Purkinje neurons (PNs) present in heterogeneous mouse cerebellar cultures. The method combines the transfection of freshly dissociated cerebellar cells via nucleofection with the use of novel expression plasmids containing a fragment of the L7 (Pcp2) gen...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.06.006
更新日期:2011-09-15 00:00:00
abstract::A sensitive, enzymatic glucose electrode was coupled with the microdialysis sampling technique to enable the continuous, on-line measurement of dialysate glucose. The glucose sensitive electrode was fabricated by immobilizing glucose oxidase onto the surface of an osmium-polyvinylpyrridine horse radish peroxidase gel ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/s0165-0270(97)00119-2
更新日期:1997-12-01 00:00:00
abstract::Brain surgery, and in particular epilepsy surgery, offers the unique opportunity to study viable human central nervous tissue in vitro. This does not only open a window to address the basic mechanisms underlying human disease, such as epilepsy, but it allows to venture into investigating neurophysiological functions p...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章,评审
doi:10.1016/j.jneumeth.2006.04.009
更新日期:2006-07-15 00:00:00
abstract::Rapid prototyping (RP) is a useful method for designing and fabricating a wide variety of devices used for neuroscience research. The present study confirms the utility of using fused deposition modeling, a specific form of RP, to produce three devices commonly used for basic science experimentation. The accuracy and ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.03.011
更新日期:2008-07-30 00:00:00
abstract::This study aimed to explore the role of surface electromyography (sEMG) on the changes of myoelectric activities of quadriceps femoris and the assessment of its clinical effect before and after selective femoral neurotomy on spastic cerebral palsy with stiff knee. Selective femoral neurotomy was carried out in 15 pati...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2011.04.031
更新日期:2011-07-15 00:00:00
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journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2009.09.016
更新日期:2009-12-15 00:00:00
abstract::A solution is described for the acquisition on a personal computer of standard pulses derived from neuronal discharge, measurement of neuronal discharge times, real-time control of stimulus delivery based on specified inter-pulse interval conditions in the neuronal spike train, and on-line display and analysis of the ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(95)00061-5
更新日期:1995-11-01 00:00:00
abstract::The tail-skin temperature is an important factor in determining tail-flick latency to noxious radiant heat in rats and mice. A simple, non-invasive method for recording the tail-skin temperature during conventional tail-flick testing is described. The method is conveniently performed during conventional tail-flick tes...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(89)90124-6
更新日期:1989-01-01 00:00:00
abstract::A loss of neostriatal neurons is a characteristic of Huntington's disease (HD), and neural tissue transplantation has been performed directly into the striatum. Since the neural stem cells have ability to migrate into the lesion site, we administered immortalized neural stem-like cells (NSC) into the ventricle or via ...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2005.09.013
更新日期:2006-04-15 00:00:00
abstract::Intra-cranial electroencephalographic recordings are increasingly employed in mice because of the availability of genetically manipulated mouse models. Currently, dental acrylic and anchoring screws are used to cement implanted electrodes. This technique works well for adult animals but often encounters difficulty whe...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2008.03.001
更新日期:2008-06-15 00:00:00
abstract:BACKGROUND:A breakthrough in the microglia and macrophages field was the identification of the macrophage colony stimulating factor-1 (CSF-1) as a pro-survival factor. Its pharmacological inhibition in animals depletes rapidly all microglia and macrophages. Microglial depletion in mixed glial cultures has always repres...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2019.108537
更新日期:2020-02-15 00:00:00
abstract::A program, written in the language Pascal, is described for signal averaging with a microcomputer. The program instructs the computer to acquire data from two channels on-line. For up to 3 channels of extracellular or intracellular recording analysed simultaneously, it was found not to be necessary to employ machine c...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/0165-0270(85)90006-8
更新日期:1985-01-01 00:00:00
abstract::Fatty acid amide hydrolase (FAAH) is the enzyme responsible for the rapid degradation of fatty acid amides such as the endocannabinoid anandamide. Inhibition of FAAH activity has been suggested as a therapeutic approach for the treatment of chronic pain, depression and anxiety, through local activation of the cannabin...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/j.jneumeth.2006.10.006
更新日期:2007-03-30 00:00:00
abstract::Interactions among receptor neurons, glial cells and neurons intrinsic to the antennal lobe of the moth underlie the formation of olfactory glomeruli. To isolate these interactions, as well as to understand the effect of a variety of humoral agents on differentiation of the neurons and glia, we generate primary cultur...
journal_title:Journal of neuroscience methods
pub_type: 杂志文章
doi:10.1016/S0165-0270(96)00025-8
更新日期:1996-10-21 00:00:00