Abstract:
:N- and K-ras mutations at codons 12 and 13 were investigated using oligonucleotide hybridization analysis after PCR amplification and subsequent sequence analysis of the amplified DNA from the region of interest in the following prostatic primary and metastatic (met) carcinoma-derived cell lines: 1013L (primary), PC3 (bone met), DU145 (brain met), and LNCaP (lymph node met). We also examined fresh and archival primary and metastatic prostate tumor tissue and benign prostatic hypertrophy specimens. All prostatic cells and tissues examined contain at least one wild-type N- and K-ras allele with respect to codons 12 and 13. No mutations were found at N-ras codon 13. The only mutation seen in the prostatic cell lines and tissues was a K-ras codon 12 position II G-to-T transversion. Since these are established nonclonal cell lines that have adapted to tissue culture, it is possible that this mutation does not represent the mutational state of prostatic carcinoma in vivo. However, the lack of consistent mutation in the ras genes amplified directly from tumors suggests that when ras mutations occur during the progression of prostatic carcinoma, they are late-stage events not directly involved in the initial development of disease. Immunoprecipitation studies using pan-ras antibodies revealed no evidence of altered expression of Ras proteins.
journal_name
Cancer Investjournal_title
Cancer investigationauthors
Pergolizzi RG,Kreis W,Rottach C,Susin M,Broome JDdoi
10.3109/07357909309020257subject
Has Abstractpub_date
1993-01-01 00:00:00pages
25-32issue
1eissn
0735-7907issn
1532-4192journal_volume
11pub_type
杂志文章abstract::The aim of our study was to investigate the role of nerve growth factor (NGF) on the expression of the p73 protein in human ependymoblastoma (EP) and medulloblastoma (MB) cells. It was found that NGF exposure on MB cells blocks proliferation, as well as on EP cells and induces overexpression of p73. NGF reduces the nu...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1080/07357900701205689
更新日期:2007-03-01 00:00:00
abstract::Most current classifications of lymphoid neoplasms define the tumors based on the cell of origin, phenotype, genetic abnormalities, and clinical features. Here it is proposed that human lymphocytic tumors can be categorized based on the propensity and capacity of the tumor cells to undergo apoptosis. The first categor...
journal_title:Cancer investigation
pub_type: 杂志文章,评审
doi:10.1081/cnv-120002951
更新日期:2002-01-01 00:00:00
abstract::Arsenic trioxide is valuable for treatment of promyelocytic leukemia, but less attention has been paid to its therapeutic potential for other cancers. In this study, the effects of arsenic trioxide were tested in human pancreatic (AsPC-1), colonic (HT-29), and breast (MCF-7) cancer cells. In all three cancer cell line...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1081/cnv-200029068
更新日期:2004-01-01 00:00:00
abstract::Decisive indulgence of angiogenesis requires a more holistic assessment and several in vivo assays have been developed that permit a more realistic appraisal of the angiogenic response. One of the most popular assays to study angiogenic activity is the chick chorioallantoic membrane (CAM). Although CAM assay is a vita...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1080/07357900600894773
更新日期:2006-10-01 00:00:00
abstract::IFN regulatory factor 4 binding protein (IBP) has been shown to play an important role in the progression of malignant tumors such as breast cancer cells, but its function in oral squamous cell carcinoma (OSCC) remains unclear. We found that IBP ectopically expressed in some OSCC specimens but not in normal oral mucos...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357907.2012.734355
更新日期:2012-12-01 00:00:00
abstract:OBJECTIVE:Patients with systemic malignancies have substantial quantities of tumor-specific DNA in their plasma which may serve as a potential biomarker for tumor burden. This approach has not been studied in gliomas. METHODS:Methylation specific polymerase chain reaction (MSP) was used to determine the methylation st...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1080/07357900500449546
更新日期:2006-02-01 00:00:00
abstract::Based on cost-effective ratio, there has not yet been imaging methods suitable for breast cancer screening in young women. The aim of this study was to evaluate the sensitivity and specificity of the combination of electrical impedance scanning (EIS) and ultrasound in identifying breast cancer for young women, to calc...
journal_title:Cancer investigation
pub_type: 杂志文章,多中心研究
doi:10.3109/07357900802203658
更新日期:2010-03-01 00:00:00
abstract::One hundred twenty-two early-stage anal canal cancer patients (median age: 69 years) were treated with curative radiotherapy with (70 patients) or without (52 patients) concomitant chemotherapy. Median follow-up was 65 months (range: 4-238). At multivariate analysis, concomitant chemotherapy significantly improved loc...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357907.2014.1001898
更新日期:2015-04-01 00:00:00
abstract::Human mesenchymal stem cells (hMSCs) play an important role in the development of human cancers. In the present study, we observed that hMSCs promoted human prostate cancer (PCa) cell PC-3 growth in vivo and in vitro. The conditional medium of hMSCs promoted the proliferation, migration, and invasion of PC-3 cells. Th...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357907.2012.692171
更新日期:2012-08-01 00:00:00
abstract::Metronomic chemotherapy refers to the chronic, equally spaced, delivery of low doses of chemotherapeutic drugs, without extended interruptions. Previously, we developed two combined metronomic schemes for the treatment of murine mammary tumors. The aim of this study was to compare their effects on tumor and metastasis...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357907.2013.877480
更新日期:2014-03-01 00:00:00
abstract::Following is a review of altered fractionation in radiation therapy for head and neck cancer. The goals of altered fractionation are to increase local-regional control and/or reduce the risk of late complications. Altered fractionation schedules can be broadly classified as accelerated fractionation and hyperfractiona...
journal_title:Cancer investigation
pub_type: 杂志文章,评审
doi:10.3109/07357909809032890
更新日期:1998-01-01 00:00:00
abstract::Recent clinical trials have demonstrated the significant cancer preventive potential of vitamin E in many different cancer sites, ranging from oral and pharyngeal cancer to prostate cancer. There is an extensive experimental basis for this clinical cancer inhibition. The experimental background includes animal studies...
journal_title:Cancer investigation
pub_type: 杂志文章,评审
doi:10.3109/07357900009031826
更新日期:2000-01-01 00:00:00
abstract::We have provided an overview of recent studies that have greatly expanded our knowledge of the molecular and cellular mechanisms that determine the sensitivity or resistance to ionizing radiation. Much of this knowledge was obtained by studying tumor and nontumor cell types that under- or overexpress proteins involved...
journal_title:Cancer investigation
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract::A natural tension exists as an IRB attempts of fulfill its mission of providing an independent and unbiased evaluation of a study while also insuring appropriate consideration of all relevant risks, benefits, and alternative strategies. IRB members with often critically relevant knowledge of unique issues involved in ...
journal_title:Cancer investigation
pub_type: 杂志文章,评审
doi:10.1080/07357900701579562
更新日期:2008-03-01 00:00:00
abstract::Placement of carmustine-impregnated wafers has become a common practice after surgical resection of malignant gliomas. Bevacizumab is used as a second-line agent for the treatment of malignant gliomas and is sometimes used in patients who have had recent wafer implantation. We describe two cases of fatal cerebrospinal...
journal_title:Cancer investigation
pub_type: 杂志文章,评审
doi:10.3109/07357907.2010.483499
更新日期:2010-12-01 00:00:00
abstract::The efficacy of dose-intense methotrexate, vinblastine, doxorubicin, and cisplatin (M-VAC) chemotherapy relative to conventional-dose M-VAC in patients with advanced transitional cell carcinoma is unknown. The outcomes of 33 patients on two successive protocols using dose-intense M-VAC with granulocyte colony-stimulat...
journal_title:Cancer investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/07357900009032829
更新日期:2000-01-01 00:00:00
abstract::We studied the production of chemiluminescence (CL) by peripheral blood neutrophils from 24 normal subjects, 13 lung cancer patients with clinical stages (CS) III and IV, and 27 breast cancer patients with CS II, III, and IV. Evaluations were made before chemo- and radiotherapy treatments. CL was expressed as: baselin...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357909309011669
更新日期:1993-01-01 00:00:00
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journal_title:Cancer investigation
pub_type: 临床试验,杂志文章,多中心研究
doi:10.3109/07357909409038230
更新日期:1994-01-01 00:00:00
abstract::Statins (3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors), cholesterol-lowering agents widely prescribed for cardiovascular health, have been shown to exert several pleiotropic effects. Although some studies reported that statins have no effects on malignancies of any kind, results of several epidemiologic ...
journal_title:Cancer investigation
pub_type: 杂志文章,评审
doi:10.1080/07357900802491444
更新日期:2009-05-01 00:00:00
abstract::Because most cancer deaths result from disseminated disease, understanding the regulation of tumor invasion and metastasis is a central theme in tumor cell biology. Interactions between extracellular matrices (ECM) and cellular microenvironment play a crucial role in this process. We have tested selected amino acids a...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357909609018433
更新日期:1996-01-01 00:00:00
abstract::To further enhance anticancer effect of Shepherdin and overcome limitation of peptide therapy, recombinant adeno-associated virus (rAAV) was constructed with following strategies: therapeutic peptide secretory expression and adeno-associated virus gene transfer system. MTT assay and flow cytometric analysis revealed t...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357900903095706
更新日期:2010-06-01 00:00:00
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journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357909409023040
更新日期:1994-01-01 00:00:00
abstract::Objectives: We examined the trends in survival based on treatment modality among non-small cell lung cancer (NSCLC) patients in the province of Ontario, Canada, from 2007 to 2015. Methods: We investigated the trends in survival based on treatment modality. Results: Among 56,417 identified patients, the mean age at dia...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1080/07357907.2019.1653465
更新日期:2019-01-01 00:00:00
abstract::The present study investigates the association between IL12Bpro and +16974A/C in 3' -untranslated region (UTR) polymorphisms of IL12B and IL-12p40 serum level related to development of colorectal cancer (CRC). Our results showed similar distribution of both the investigated polymorphisms among CRC patients and healthy...
journal_title:Cancer investigation
pub_type: 杂志文章,多中心研究
doi:10.3109/07357900902918486
更新日期:2009-11-01 00:00:00
abstract::The expression of the plasma membrane Ca(2+) ATPase (PMCA) was analyzed in a series of 84 formalin-fixed and paraffin embedded colon samples including normal mucosa (n = 32), adenoma (n = 19), adenocarcinoma (n = 27), and lymph node metastasis (n = 6) using (i) immunohistochemistry, (ii) mRNA in situ hybridization, an...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357907.2012.657817
更新日期:2012-05-01 00:00:00
abstract::Despite increasing evidence of benefit from adjuvant chemotherapy, older women with breast cancer are commonly given less aggressive treatment than younger patients. Conflicting prior data regarding age-related toxicity prompted this prospective study. Forty-four women (aged 35-79 years) with early-stage breast cancer...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.3109/07357900009012191
更新日期:2000-01-01 00:00:00
abstract::CXCL12 is an extracellular chemokine binding to cell surface receptor CXCR4. We found that activation of CXCL12/CXCR4 axis stimulated angiogenesis in endothelial cells. Knockdown of CXCR4 in endothelial cells prevented the branch points of angiogenesis. Endothelial cells exposed to CXCL12 presented high level of epide...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1080/07357907.2017.1422512
更新日期:2018-01-02 00:00:00
abstract::A series of 3-aryl/hetarylquinoxaline-2-carbonitrile-1,4-dioxides was synthesized and evaluated against breast cancer cell lines in normoxia and hypoxia. Selected compounds in this series demonstrated better cytotoxicity and comparable hypoxia selectivity than tirapazamine. In contrast to Dox, quinoxaline-1,4-dioxides...
journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1080/07357907.2018.1453072
更新日期:2018-03-16 00:00:00
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journal_title:Cancer investigation
pub_type: 杂志文章
doi:10.1080/07357900600970490
更新日期:2007-02-01 00:00:00
abstract:BACKGROUND:Hand-foot syndrome (HFS) has been previously reported as a side effect in 45-56% of patients treated with capecitabine. However, the natural history of HFS has not been fully characterized. In this study, we investigate the incidence, severity, and time course of HFS. DESIGN:Toxicity data collected for 41 p...
journal_title:Cancer investigation
pub_type: 临床试验,杂志文章
doi:10.1081/cnv-120000360
更新日期:2002-01-01 00:00:00