Abstract：
:Stimulation of osteoblast proliferation and differentiation is important for the in vivo bone-bonding ability of biomaterials. Previous in vitro studies have used biochemical assays to analyze osteoblast-specific gene expression in cultured osteoblasts. In this study, we generated transgenic mice harboring a monomeric red fluorescent protein 1 transgene under the control of a 2.3-kb fragment of the Col1a1 promoter, which is active specifically in osteoblasts and osteocytes. We established a fluorescent primary osteoblast culture system to allow noninvasive observation of osteoblast proliferation and differentiation on opaque materials in vitro. We used this system to evaluate alkali- and heat-treated titanium, which has a strong bone-bonding ability in vivo, and we observed a rapid increase in fluorescence intensity and characteristic multifocal nodule formation. A cell proliferation assay and RT-PCR to examine osteoblast-specific gene expression showed increased osteoblast proliferation and differentiation consistent with the fluorescence observations. This mouse model allowed us to use fluorescence intensity to visualize and quantify in vivo newly formed bone around implanted materials in femurs. The use of these fluorescent osteoblasts is a promising method for simple screening of the bone-bonding ability of new materials.
journal_name
J Bone Miner Metabjournal_title
Journal of bone and mineral metabolismauthors
Tsukanaka M,Yamamoto K,Fujibayashi S,Pattanayak DK,Matsushita T,Kokubo T,Matsuda S,Akiyama Hdoi
10.1007/s00774-013-0544-8subject
Has Abstractpub_date
2014-11-01 00:00:00pages
660-70issue
6eissn
0914-8779issn
1435-5604journal_volume
32pub_type
杂志文章abstract：:In 50 premenarcheal girls selected from the lowest and highest end of the calcium-intake distribution of a large population sample, we evaluated bone mineral density (BMD), together with the following hormonal-metabolic parameters: androstenedione (ASD), dehydroepiandrosterone (DHEA), dehydroepiandrosterone sulfate (D...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-003-0451-5
更新日期：2004-01-01 00:00:00
abstract：:Advanced glycation end products (AGE) in collagen have been reported to decrease the mechanical property of bone. However, there are no available data on the relation between fracture risk and levels of glycoxidative (nonenzymatic) cross-links of collagen in clinical samples. A total of 432 Japanese elderly women who ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-007-0784-6
更新日期：2008-01-01 00:00:00
abstract：:Bone development up to early adulthood plays an important role in determining the risk of osteoporosis later in life. However, bone development in children has not been fully documented by longitudinal studies in Japanese children. The purpose of this study is to determine the degree of tracking of areal bone mineral ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-010-0213-0
更新日期：2011-03-01 00:00:00
abstract：:High serum homocysteine (HCY) and indirectly deficiency of folate and/or vitamin B(12) stimulate bone resorption and adversely affect collagen cross-linking. The aim of this study was the evaluation of serum levels of HCY, folate and vitamin B(12) in patients with Paget's disease of bone (PDB) and the effect of zoledr...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-009-0131-1
更新日期：2010-05-01 00:00:00
abstract：:Chronic kidney disease (CKD) mineral and bone disorders (CKD-MBD) may lead to low bone mineral density (BMD) and vascular calcification (VC), but links to the latter are unclear. Here we investigated associations between BMD, coronary artery calcium (CAC) scores, and histological signs of VC in end-stage renal disease...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-016-0788-1
更新日期：2017-09-01 00:00:00
abstract：:Recent studies have suggested that a high level of recent physical activity increases the risk of a wrist fracture in postmenopausal women. The relationship of more distant past physical activity to wrist fracture is less clear, and most studies have relied on recall of physical activity much earlier in life. The aim ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-006-0715-y
更新日期：2006-01-01 00:00:00
abstract：:Osteopathic changes sometimes occur in patients with complex regional pain syndrome (reflex sympathetic dystrophy and causalgia). We aimed to investigate whether such osteopathic changes occurred in rats with chronic constriction injury (CCI) of the sciatic nerve. A CCI of the sciatic nerve was established in a unilat...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s007740200012
更新日期：2002-01-01 00:00:00
abstract：:The present study aimed to evaluate the prevalence and clinical presentation of Paget's disease of bone (PDB) in Japan. As PDB is a very rare disease in Japan, a nationwide mail survey was conducted targeting doctors in the specialty most frequently diagnosing and treating PDB patients in Japan. First, the literature ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-005-0670-z
更新日期：2006-01-01 00:00:00
abstract：:We have previously demonstrated the efficacy of therapeutic exercise for osteoarthritis (OA) of the knee. This study was performed to examine the additive effects of glucosamine or risedronate on the exercise therapy. In this study, 142 female patients with moderate OA of the knee, who had been recommended to undergo ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00774-007-0813-5
更新日期：2008-01-01 00:00:00
abstract：:The aim of the study was to assess the clinical performance of the model combining areal bone mineral density (aBMD) at spine and microarchitecural texture (TBS) for the detection of the osteoporotic fracture. The Eastern European Study is a multicenter study (Serbia, Bulgaria, Romania and Ukraine) evaluating the role...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,多中心研究
doi：10.1007/s00774-013-0529-7
更新日期：2014-09-01 00:00:00
abstract：:Various animal experiments and human studies have shown that intermittent injections of parathyroid hormone (PTH) exert anabolic effects on bone, whereas continuous PTH treatment decreases the bone mass and causes hypercalcemia in animals. However, limited data are available with regard to the effects of a repetitive ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-010-0181-4
更新日期：2010-11-01 00:00:00
abstract：:Osteoporosis is a growing health problem in women and in men. This cross-sectional study examined the association of anthropometric, lifestyle, and hormonal factors with bone mineral density (BMD) in 152 healthy Korean middle-aged men. Smoking habits and alcohol consumption were assessed by interview. Serum testostero...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-003-0500-0
更新日期：2004-01-01 00:00:00
abstract：:We evaluated adherence with raloxifene therapy compared with daily bisphosphonate in Asian postmenopausal women at increased risk of osteoporotic fractures. In this 12-month observational study conducted in Asia (Hong Kong, Malaysia, Pakistan, Philippines, Singapore, Taiwan), 984 postmenopausal women (aged 55 years or...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-006-0735-7
更新日期：2007-01-01 00:00:00
abstract：INTRODUCTION:Etelcalcetide binds to the extracellular domain of the calcium-sensing receptor (CaSR), while cinacalcet binds to the 7-transmembrane domain of the CaSR; however, it is unknown, whether etelcalcetide has similar effects to cinacalcet on parathyroid hormone (PTH) secretion. MATERIALS AND METHODS:The PTH-ca...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-020-01169-z
更新日期：2020-11-16 00:00:00
abstract：:There are safety concerns regarding administration of bisphosphonates to children. Little is known about the effects of bisphosphonates on bone matrix organization during bone modeling. The present study examined the effects of alendronate (ALN) on bone matrices formed by intramembranous ossification in the appendicul...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-007-0782-8
更新日期：2008-01-01 00:00:00
abstract：:We evaluated short-term changes in serum amino-terminal procollagen propeptide (P1NP) and cross-linked C-terminal telopeptide (betaCTX) of type I collagen after parathyroidectomy (PTX) in 41 postmenopausal women with primary hyperparathyroidism (PHPT). Serum levels of 25-hydroxyvitamin D, intact PTH, calcium, phosphat...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-008-0033-7
更新日期：2009-01-01 00:00:00
abstract：:In end-stage osteoarthritis (OA) of the hip, the effect of bone metabolism with and without cartilage is unclear. In this study, we aimed to investigate histomorphology and microdamage in the subchondral bone of the femoral head in areas with and without articular cartilage in patients with end-stage OA. Nineteen femo...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-019-00988-z
更新日期：2019-09-01 00:00:00
abstract：:In Caucasian populations, the polymorphic restriction endonuclease HindIII marker of the osteocalcin (also known as BGP, for bone Gla protein) gene has recently been reported to be associated with bone mass, a major risk determinant of osteoporosis. In this study, we investigated the relationship between the BGP HindI...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-003-0478-7
更新日期：2004-01-01 00:00:00
abstract：:In the course of complex regional pain syndrome (CRPS), local osteopenia in the subchondral/subcortical areas of the affected limb represents a central manifestation. Mechanistic aspects of CRPS-associated pathologies remain unclear, and knowledge about bone morphology in CRPS-affected areas is rare. The aim of this s...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-018-0976-2
更新日期：2019-07-01 00:00:00
abstract：:Simvastatin has been shown to stimulate osteogenesis both in vitro and in vivo. However, the mechanism by which simvastatin exerts its effects is still unclear. We previously reported that simvastatin promotes bone morphogenetic protein 2 (BMP-2) expression, induces osteoblastic differentiation, and inhibits adipocyti...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-007-0820-6
更新日期：2008-01-01 00:00:00
abstract：INTRODUCTION:Aromatase inhibitors are known to accelerate bone loss in patients with breast cancer. However, how much AIs affect the efficacy of antiresorptive agents has not been studied. The study aimed to compare the effect of alendronate on bone mineral density (BMD) between patients with and without AI treatment. ...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-020-01111-3
更新日期：2020-09-01 00:00:00
abstract：:Osteonecrosis of the jaw (ONJ) associated with bisphosphonate therapy is a rare but severe side effect in osteoporosis patients. Recently, the number of osteoporosis patients with ONJ has dramatically increased in Japan. This has contributed to an increase in the number of patients avoiding extractions. However, there...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00774-019-00990-5
更新日期：2019-09-01 00:00:00
abstract：:Endoplasmic reticulum (ER) stress has been reported to be linked to various diseases such as diabetes, neurodegenerative diseases, and osteogenesis imperfecta (OI). Old astrocyte specifically induced substance (OASIS), a novel type of ER stress transducer, is a basic leucine zipper transcription factor belonging to th...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-009-0142-y
更新日期：2010-07-01 00:00:00
abstract：:Lactoferrin (Lf) expression was investigated by using a Lf monoclonal antibody in 50 formalin-fixed and paraffin-embedded human bone tumours [10 giant cell tumours (GCTs), 7 osteoid osteomas, 6 ossifying fibromas, 19 enchondromas, 2 chondroblastomas, 2 chondrosarcomas, 2 chondroblastic osteosarcomas, 1 myeloma and 1 a...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-009-0044-z
更新日期：2009-01-01 00:00:00
abstract：INTRODUCTION:Physical activity (PA) is a key factor of bone mass acquisition in peripubertal children. Sedentary behavior (SB) has been shown to influence bone outcomes. This study aimed to examine the association between objectively measured PA and SB and bone stiffness in Japanese children. MATERIALS AND METHODS:Par...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-019-01021-z
更新日期：2019-11-01 00:00:00
abstract：:The effects of catecholamine excess due to pheochromocytoma on body composition, including skeletal muscle mass, are unknown. Here, we investigated the effects of catecholamine metabolites on body composition in subjects with pheochromocytoma. After body compositions using bioelectrical impedance analysis, urinary met...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00774-018-0959-3
更新日期：2019-07-01 00:00:00
abstract：:Sarcopenia is an age-related loss of skeletal muscle mass and strength. It has been widely recognized that low muscle mass was essential in the diagnosis of sarcopenia, whereas recent studies have emphasized the importance of muscle strength. In practice, muscle quality as well as muscle mass might determine the stren...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章,评审
doi：10.1007/s00774-019-01048-2
更新日期：2020-01-01 00:00:00
abstract：:In this study, we tested the interleukin-6 (IL-6) gene as an important candidate gene for its linkage and association with the variation of bone phenotypes (bone mineral density [BMD] and bone size) in young Chinese female subjects. We genotyped the IL-6 gene at the -634C/G restriction fragment length polymorphism (RF...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-004-0607-y
更新日期：2005-01-01 00:00:00
abstract：:The prevalence and pattern of joint involvement in radiographic hand osteoarthritis (OA) have been reported in Western populations, but similar data are lacking for Japanese. We examined this issue in 551 Japanese women aged > or = 40 years. Radiographs were obtained of both hands and graded according to the Kellgren-...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-006-0693-0
更新日期：2006-01-01 00:00:00
abstract：:Chronic liver disease in adults is a risk factor of osteoporosis, but little is known about risk of fractures in children with non-cholestatic liver disease. The aim of this study was to investigate associations among the severity of liver fibrosis, bone mass and low-energy fractures in children. History of fractures,...
journal_title：Journal of bone and mineral metabolism
pub_type： 杂志文章
doi：10.1007/s00774-010-0219-7
更新日期：2011-05-01 00:00:00
