Abstract:
:Hemorrhagic enteritis virus (HEV), a type II avian adenovirus, causes intestinal hemorrhages and immunosuppression in turkeys. In this study, we exposed turkeys to virulent HEV and examined fractionated spleen cells for the presence of viral DNA by in situ hybridization and amplification of DNA extracted from virus-infected cells by PCR. HEV replication was detected only in the immunoglobulin M-bearing B lymphocytes and macrophage-like cells but not in the CD4+ or CD8+ T lymphocytes. The inability to infect T cells distinguishes type II avian adenoviruses from lymphotropic mammalian adenoviruses which infect and replicate in T cells. Furthermore, these data suggested that HEV-induced immunosuppression in turkeys may be due to the effect of the virus on B lymphocytes and macrophages. We also examined tissue tropism of HEV by in situ hybridization conducted on sections of lymphoid and nonlymphoid tissues. Large numbers of HEV-positive cells were detected in spleen and cecal tonsils. Diminutive viral activity was present in the intestines, the principal site of HEV-induced pathology. Thus, intestinal pathology was not associated with local cytopathic viral replication. This result and our previous observation that cyclosporin A abrogated intestinal hemorrhaging in HEV-infected turkeys strongly suggested that intestinal lesion induced by this virus may be immune system mediated.
journal_name
J Viroljournal_title
Journal of virologyauthors
Suresh M,Sharma JMdoi
10.1128/JVI.70.1.30-36.1996subject
Has Abstractpub_date
1996-01-01 00:00:00pages
30-6issue
1eissn
0022-538Xissn
1098-5514journal_volume
70pub_type
杂志文章abstract::Analysis of the pronase-derived glycopeptides of isolated mumps virus glycoproteins revealed the presence of both complex and high-mannose-type oligosaccharides on the HN and F1 glycoproteins, whereas only high-mannose-type glycopeptides were detected on F2. Endoglycosidase F, a newly described glycosidase that cleave...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.2.354-362.1983
更新日期:1983-08-01 00:00:00
abstract::The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit of the viral RNA-dependent RNA polymerase complex. The protein is phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within the amino-terminal acidic domain I of P protein ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.73.7.5613-5620.1999
更新日期:1999-07-01 00:00:00
abstract::The ICP18.5 gene (UL28) of herpes simplex virus type 1 is a member of a well-conserved gene family among herpesviruses and is thought to play a role in localization of viral glycoproteins. We have cloned, sequenced, and expressed the entire pseudorabies virus (PRV) ICP18.5 open reading frame in Escherichia coli as a C...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.7.3746-3758.1991
更新日期:1991-07-01 00:00:00
abstract::The paramyxoviral family contains many medically important viruses, including measles virus, mumps virus, parainfluenza viruses, respiratory syncytial virus, human metapneumovirus, and the deadly zoonotic henipaviruses Hendra and Nipah virus (NiV). To both enter host cells and spread from cell to cell within infected ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01469-16
更新日期:2016-11-14 00:00:00
abstract::The human papillomavirus type 18 (HPV-18) upstream regulatory region (URR) controls viral gene transcription in a cell-type-specific manner. The HPV-18 URR is active in HeLa cells but inactive in HepG2 cells. The activating activity of YY1 in HeLa cells is dependent on its functional interactions with the switch regio...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.11.7695-7705.1996
更新日期:1996-11-01 00:00:00
abstract::Poliovirus (PV) modifies membrane-trafficking machinery in host cells for its viral RNA replication. To date, ARF1, ACBD3, BIG1/BIG2, GBF1, RTN3, and PI4KB have been identified as host factors of enterovirus (EV), including PV, involved in membrane traffic. In this study, we performed small interfering RNA (siRNA) scr...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00114-12
更新日期:2012-05-01 00:00:00
abstract::Preclinical studies have shown that gene transfer following readministration of viral vectors is often inefficient due to the presence of neutralizing antibodies. Vectors derived from ubiquitous human adenoviruses may have limited clinical use because preexisting humoral and cellular immunity is found in 90% of the po...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.74.1.505-512.2000
更新日期:2000-01-01 00:00:00
abstract::The cellular requirements for activation of herpesvirus fusion and entry remain poorly understood. Low pH triggers change in the antigenic reactivity of the prefusion form of the herpes simplex virus (HSV) fusion protein gB in virions, both in vitro and during viral entry via endocytosis (S. Dollery et al., J. Virol. ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05291-11
更新日期:2011-10-01 00:00:00
abstract:UNLABELLED:Classical swine fever virus (CSFV) is the causative agent of classical swine fever (CSF), a highly contagious, economically important viral disease in many countries. The E(rns) and E2 envelope glycoproteins are responsible for the binding to and entry into the host cell by CSFV. To date, only one cellular r...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00019-15
更新日期:2015-05-01 00:00:00
abstract::The EFII cis element is a 38-bp sequence at the 5' end of the Rous sarcoma virus long terminal repeat, extending from nucleotides -229 to -192 (with respect to the viral transcription start site), which is recognized by sequence-specific DNA-binding proteins in avian fibroblast nuclear extracts (L. Sealy and R. Chalkl...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.66.11.6338-6352.1992
更新日期:1992-11-01 00:00:00
abstract::Papillomaviruses (PVs) are a large family of small DNA viruses infecting mammals, reptiles, and birds. PV infection induces cell proliferation that may lead to the formation of orogenital or skin tumors. PV-induced cell proliferation has been related mainly to the expression of two small oncoproteins, E6 and E7. In ma...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01777-08
更新日期:2009-09-01 00:00:00
abstract::To understand the roles of heptad repeat 1(HR1) and HR2 of the spike (S) protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) in virus-cell interactions, the conserved Leu or Ile residues located at positions 913, 927, 941, and 955 in HR1 and 1151, 1165, and 1179 in HR2 were individually replaced wi...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.80.7.3225-3237.2006
更新日期:2006-04-01 00:00:00
abstract::Hepatitis C virus core protein forms the viral nucleocapsid and plays a critical role in the formation of infectious particles. In this study, we demonstrate that the highly conserved residue G33, located within domain 1 of the core protein, is important for the production of cell culture-infectious virus (HCVcc). Ala...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05452-11
更新日期:2012-01-01 00:00:00
abstract::We have detected a mouse mammary tumor virus (MMTV)-specific 1.7-kilobase (kb) polyadenylated RNA in mammary glands of several mouse strains. In BALB/c mice, it is the only MMTV-specific RNA species present. C3H and GR mammary glands and tumors contain, in addition, 3.8- and 7.8-kb MMTV RNAs. Nuclease S1 analysis was ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.46.2.362-370.1983
更新日期:1983-05-01 00:00:00
abstract::The unexpectedly low efficacy of influenza vaccine during school outbreaks of influenza B virus in the spring of 1987 in Japan was probably attributable to a poor antibody response of vaccinees to the epidemic viruses. An antigenic analysis of the causative B viruses isolated in 1987 and 1988 showed much variation in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.64.6.2860-2865.1990
更新日期:1990-06-01 00:00:00
abstract::Both the RNase H domain of Moloney murine leukemia virus (Mo-MLV) reverse transcriptase (RT) and Escherichia coli RNase H possess a positively charged alpha-helix (C helix) and a loop that are not present in the RNase H domains of human immunodeficiency virus (HIV) RT or avian sarcoma virus RT. Although a mutant Mo-ML...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.16.8360-8373.2002
更新日期:2002-08-01 00:00:00
abstract::The 3'-end host-virus junction fragments from two bovine leukemia virus (BLV)-induced lymphoid tumors (tumors 15-4 and 1351), each containing a single provirus, were used as probes to detect large restriction fragments flanking these proviruses. The DNAs from 28 other independent BLV-induced tumors were checked by Sou...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.47.1.146-150.1983
更新日期:1983-07-01 00:00:00
abstract::The efficiency of processing of polyoma viral RNA and of its export from nucleus to cytoplasm was measured in primary mouse kidney cells by comparing the initial rates of incorporation of [3H]uridine into cytoplasmic and nuclear viral RNA. Appropriate methods of cell fractionation were chosen to maximize yields of cyt...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.37.2.628-635.1981
更新日期:1981-02-01 00:00:00
abstract::Lentivectors stimulate potent immune responses to antigen transgenes and are being developed as novel genetic vaccines. To improve safety while retaining efficacy, we constructed a lentivector in which transgene expression was restricted to antigen-presenting cells using the mouse dectin-2 gene promoter. This lentivec...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01289-07
更新日期:2008-01-01 00:00:00
abstract::The matrix (M) protein of vesicular stomatitis virus (VSV) is a potent inhibitor in vivo of transcription by all three host RNA polymerases (RNAP). In the case of host RNA polymerase II (RNAPII), the inhibition is due to lack of activity of the TATA-binding protein (TBP), which is a subunit of the basal transcription ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.9.4453-4458.2001
更新日期:2001-05-01 00:00:00
abstract::Theiler's virus infection of the central nervous system (CNS) induces an immune-mediated demyelinating disease in susceptible mouse strains, such as SJL/J, and serves as a relevant infectious model for human multiple sclerosis. It has been previously suggested that susceptible SJL/J mice do not mount an efficient cyto...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.13.6577-6585.2002
更新日期:2002-07-01 00:00:00
abstract::Many viruses induce type I interferon responses by activating cytoplasmic RNA sensors, including the RIG-I-like receptors (RLRs). Although two members of the RLR family, RIG-I and MDA5, have been implicated in host control of virus infection, the relative role of each RLR in restricting pathogenesis in vivo remains un...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01403-13
更新日期:2013-11-01 00:00:00
abstract::The family Caliciviridae is divided into four genera and consists of single-stranded RNA viruses with hosts ranging from humans to a wide variety of animals. Human caliciviruses are the major cause of outbreaks of acute nonbacterial gastroenteritis, whereas animal caliciviruses cause various host-dependent illnesses w...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.78.12.6469-6479.2004
更新日期:2004-06-01 00:00:00
abstract::Newborn mice suckled on dams immunized either orally or parenterally with primate rotavirus SA-11 were protected against diarrhea induced by SA-11 virus challenge. Experimental oral administration of milk from orally immunized dams protected suckling mice against challenge; protective activity was detected both in the...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.54.1.58-64.1985
更新日期:1985-04-01 00:00:00
abstract::Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00601-08
更新日期:2008-07-01 00:00:00
abstract::The products of the A33R and A36R genes of vaccinia virus are incorporated into the membranes of intracellular enveloped virions (IEV). When extracts of cells that had been infected with vaccinia virus and labeled with H(3)(32)PO(4) were immunoprecipitated with antibodies against the A33R protein, two prominent bands ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.1.303-310.2001
更新日期:2001-01-01 00:00:00
abstract::The TAATGARAT motif in the herpes simplex virus (HSV) immediate-early (IE) gene promoters plays a key role in their activation by the Oct-1-Vmw65 complex, but its role in mediating inhibitory effects of cellular octamer-binding proteins is less clear. We have used indicator viruses containing reporter constructs with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.4.3495-3500.1998
更新日期:1998-04-01 00:00:00
abstract::Murine coronavirus mouse hepatitis virus (MHV) causes persistent infection of the central nervous system (CNS) in rodents, which has been associated with demyelination. However, the precise mechanism of MHV persistence in the CNS remains elusive. Here we show that the progenitor oligodendrocytes (central glial 4 [CG-4...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.1.47-56.2005
更新日期:2005-01-01 00:00:00
abstract::Molluscs, comprising one of the most successful phyla, lack clear evidence of adaptive immunity and yet thrive in the oceans, which are rich in viruses. There are thought to be nearly 120,000 species of Mollusca, most living in marine habitats. Despite the extraordinary abundance of viruses in oceans, molluscs often h...
journal_title:Journal of virology
pub_type: 杂志文章,评审
doi:10.1128/JVI.00287-15
更新日期:2015-08-01 00:00:00
abstract::The HTLV-1 oncoprotein Tax is a potent activator of classical and alternative NF-κB pathways and is thought to promote cell proliferation and transformation via NF-κB activation. We showed recently that hyperactivation of NF-κB by Tax triggers a cellular senescence response (H. Zhi et al., PLoS Pathog. 7:e1002025, 201...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00158-12
更新日期:2012-09-01 00:00:00