Abstract：
:The initial stage of atherosclerotic plaque formation involves oxidation of the phosphatidyl-choline moiety of low density lipoprotein (LDL) and subsequent uptake by macrophages. Ongoing uptake in developing plaque also may involve oxidized LDL and would require an oxidizing environment in plaque lipids. Atherosclerotic plaque lipids from 12 patients undergoing peripheral vascular procedures were extracted in chloroform: methanol (2:1). This extract was applied to a 25 cm 5 micron silica HPLC column and eluted with a ternary gradient mobile phase utilizing a laser light scattering (ELSD) mass detector. Individual lipid fractions were then analyzed. Cholesterol, both free and esterified, was the most prominent lipid in plaque (104 +/- 74 mg/gm tissue. However, lipid peroxides were present in much higher concentrations (3.52 +/- 2.84 FU X 10(4)/mg phospholipid) and overall level (21.27 +/- 10.10 FU X 10(4)/gm plaque) in the phospholipid component (*p< 0.05). Phosphatidyl-choline (PC) accounted for 63% of the total phospholipid peroxides recovered (6.31 +/- 5.09 mg/gm plaque; *p<0.05). PC and phosphatidylinositol (PI) content were linearly related to lipid peroxide fluorescence (PC; r=0.696; p=0.01) (PI; r=0.809; p=0.001). Lipid peroxides in human atherosclerotic plaque are present primarily in the phospholipid component and phosphatidyl-choline forms the bulk of these peroxides. PC may play an important role in ongoing plaque lipid accumulation.
journal_name
Life Scijournal_title
Life sciencesauthors
Piotrowski JJ,Shah S,Alexander JJdoi
10.1016/0024-3205(95)02351-8subject
Has Abstractpub_date
1996-01-01 00:00:00pages
735-40issue
9eissn
0024-3205issn
1879-0631pii
0024320595023518journal_volume
58pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Pretreatment of pancreatic acini with 5-hydroxytryptamine (5-HT) reduced the binding of the labeled somatostatin (SS) analogue 125I-Tyr3-SMS to pancreatic acinar membranes. This effect was dependent of the dose of 5-HT used and length of pretreatment. This inhibitory effect of 5-HT was abolished when pancreatic acini ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00161-6
更新日期：1997-01-01 00:00:00
abstract：:Using liposomes differing in size and lipid composition, we have studied the uptake characteristics of the liver parenchymal and Kupffer cells. Desferal labeled with iron-59 was chosen as a radiomarker for the liposomal content, because Desferal in its free form does not cross cellular membranes. At various time inter...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90098-4
更新日期：1982-11-08 00:00:00
abstract：:Free radicals induced by cerebral ischemia-reperfusion injury can trigger lipid peroxidation, leading to the production of malondialdehyde (MDA) and 4-hydroxy-2(E)-nonenal (4-HNE). Post-ischemia electroacupuncture (EA) therapy was able to reduce extent of lipid peroxidation. However, the effect of pre-ischemic EA ther...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.02.025
更新日期：2004-07-30 00:00:00
abstract：:Gastrin is mitogenic for several colon cancers and is postulated as an autocrine growth factor for colon cancer cells. In the present study we report the development of a simple competitive polymerase chain reaction (PCR) method for measuring relative abundance of gastrin gene expression in colon cancer cells. Primers...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00550-8
更新日期：1994-01-01 00:00:00
abstract：:The effect of the repeated or continuous administration of an analog of GH releasing factor (GH-RF), D-Tyr-1, D-Ala-2, Nle-27, GH-RF(1-29)-NH2 (DBO-29), on the subsequent response to this peptide was investigated in pentobarbital-anesthetized male rats. A sc administration of this analog induced a greater and more pro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90759-4
更新日期：1987-06-22 00:00:00
abstract：:Positive inotropic and chronotropic responses of guinea-pig isolated left and right atria to sympathomimetic amines were examined at bath temperatures of 38, 30 or 25 degrees C. The concentration-response curves to isoproterenol and orciprenaline were displaced to the left by cooling, indicating hypothermia-induced su...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90074-2
更新日期：1984-09-03 00:00:00
abstract：:Recent evidence suggests that the Type 1 opiate receptor (in rat striatal patches) is a mobile receptor which is able to adopt a mu, delta, or kappa opiate receptor ligand selectivity pattern under appropriate conditions. In this paper, we have investigated such a possibility for Type 2 opiate receptors which are visu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90183-7
更新日期：1982-10-18 00:00:00
abstract：:Addition of synthesized atriopeptin II (AP-2), a 23 amino acid peptide of rat atria, to rat thoracic aorta smooth muscle cells results in the stimulation of cyclic GMP production by the cells. The EC50 for the effect is 81 nM and a 7 fold increase occurs at 10 microM AP-2. Cyclic GMP levels increased within 15 seconds...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90425-4
更新日期：1985-08-19 00:00:00
abstract：:Studies were performed in conscious, chronically catheterized male Sprague-Dawley rats to investigate the effect of administered atrial natriuretic peptide (ANP) on blood pressure, renal hemodynamics and urinary electrolyte excretion. Studies were performed on young adult (3-4 month old) rats and on aging rats (18-24 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90066-z
更新日期：1990-01-01 00:00:00
abstract：:The mechanism of fibrate-induced myopathy was investigated in this report. When clofibrate (30 to 300 microM) was applied to L6 rat skeletal myoblasts, dose-dependently apoptosis was observed within 24 h. In the apoptotic myoblasts, a caspase-12 cleavage was observed at 2 h and with following caspases-9 and -3-related...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.08.003
更新日期：2006-03-13 00:00:00
abstract：:The interactions of aminoglycoside, 3',4'-dideoxykanamycin B(DKB) with ATP and its related compounds were investigated. ATP, ADP, cyclic AMP and FAD bound to the DKB-conjugated Sepharose 4B column. The binding of DKB to ATP was also confirmed by equilibrium gel filtration. In the acidic pH region, the fluorescence of ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90332-2
更新日期：1986-10-13 00:00:00
abstract：AIMS:GnRH-DFF40 (gonadotropin releasing hormone-DNA fragmentation factor 40) humanized recombinant immunotoxin serves as a prospective candidate for targeted therapy of malignancies with over-expressed gonadotropin releasing hormone receptor (GnRHR). In this study, we attempted to generate a GnRH-based chimeric protein...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118866
更新日期：2021-01-15 00:00:00
abstract：:The effect of 32 mM ascorbate on the time courses of phosphocreatine (PCr), inorganic phosphate (Pi), adenosine triphosphate (ATP) and intracellular pH in rat skeletal muscle during ischemia and reperfusion was investigated in vivo using 31P nuclear magnetic resonance (NMR) spectroscopy. Ascorbate was administered int...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00904-x
更新日期：1995-01-01 00:00:00
abstract：:Male and female rats with two permanently indwelling intravenous catheters were infused for 2 hours with ovine prolactin. During equilibrium conditions the effects of intravenously injected L-DOPA and benzerazide (a blocker of dopa-decarboxylase) on steady state levels of ovine prolactin were measured. A dose of 4.5 m...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90549-5
更新日期：1982-08-30 00:00:00
abstract：:The effects of GABA antagonists, picrotoxin and bicuculline were studied on hyperphagia caused by insulin in free feeding rats. It was found that peripheral administration of GABA antagonists inhibited this hyperphagia. These agents were administered in the VMH and LH through stereotaxically implanted cannulae. It was...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90220-0
更新日期：1984-06-04 00:00:00
abstract：:Plants are potentially important for novel therapeutic drug leads, but the slowness of conventional methods for investigation of plants limits enthusiasm in the pharmaceutical industry. To overcome some of the drawbacks, we have applied high throughput pharmacological screening (HTPS) to crude plant extracts. Using a ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.09.013
更新日期：2005-12-22 00:00:00
abstract：:The antioxidative and superoxide- and hydroxyl radical-scavenging activities and pro-oxidant effect of twelve selected medicinal herbs were studied. The aqueous extracts of Coptis chinensis, Paeonia suffruticosa, Prunella vulgaris and Senecio scandens exhibited the highest potency in inhibiting rat erythrocyte hemolys...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00643-8
更新日期：2000-01-14 00:00:00
abstract：:The impact of low level lead exposure on human central nervous system function is a major public health concern. This study addresses the inhibition of the cation pump enzyme Na, K-ATPase by low level lead. Human brain tissue was obtained at autopsy and frozen until use. Brain homogenates were preincubated with PbCl2 ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90148-5
更新日期：1991-01-01 00:00:00
abstract：:The purpose of this study was to determine the effects of the 7288ctc Morris hepatoma on heart size and performance. Hearts from tumor bearing and control animals were perfused in the working configuration one to three weeks post implantation. As tumor growth progressed there was an inverse linear relationship between...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00819-1
更新日期：1994-01-01 00:00:00
abstract：:Experiments were done to examine the analgesic effect of thiorphan alone or in combination with stress in mice. Analgesia was assessed by measuring jump latencies from a 55 degrees C hot plate. Thiorphan exhibited weak analgesic properties evidenced by significant increases in jump latencies only after 300 mg/kg i.p. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90338-1
更新日期：1982-09-20 00:00:00
abstract：AIMS:Insulin receptor signaling in osteoblasts has been well established, but the effects of insulin on osteoclast proliferation are poorly explored. The objective of this study was to investigate the roles and the mechanisms of insulin on osteoclast proliferation. MAIN METHODS:After insulin treatment to primary osteo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2014.07.006
更新日期：2014-10-02 00:00:00
abstract：:Aspartate (ASP), glutamate (GLU), noradrenaline (NA), dopamine (DA) and its acidic metabolites DOPAC and HVA, serotonin (5-HT) and its metabolite 5-HIAA were simultaneously investigated in post-mortem tissue samples from right parahippocampal gyrus (temporal cortex) and frontal cortical pole (frontal cortex) of adults...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00067-2
更新日期：1997-01-01 00:00:00
abstract：:Bone matrix turnover is regulated by matrix metalloproteinases (MMPs), tissue inhibitors of matrix metalloproteinases (TIMPs), and the plasminogen activation system, including tissue-type plasminogen activator (tPA), urokinase-type plasminogen activator (uPA), and plasminogen activator inhibitor type-1 (PAI-1). We pre...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2006.01.040
更新日期：2006-07-04 00:00:00
abstract：:The endogenous cannabinoid anandamide (AEA) exerts the majority of its effects at CB1 and CB2 receptors and is degraded by fatty acid amide hydrolase (FAAH). FAAH KO mice and animals treated with FAAH inhibitors are impaired in their ability to hydrolyze AEA and other non-cannabinoid lipid signaling molecules, such as...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2012.05.015
更新日期：2013-03-19 00:00:00
abstract：:Nitric oxide (NO) is generated from L-arginine by different isoforms of the enzyme nitric oxide synthase (NOS) and is known to be involved in mediating several biological functions, some of which are associated with reproduction. In this study, we examined the ability of the prepubertal ovary to express the inducible ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00710-8
更新日期：1997-01-01 00:00:00
abstract：AIMS:Asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase inhibitor, has been reported to be a novel marker for the progression of chronic kidney disease (CKD). We have recently found that accumulation of ADMA could trigger peritubular capillary loss, thus contributing to tubulointerstitial ischemia ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2009.03.018
更新日期：2009-06-05 00:00:00
abstract：:Intravenous injection of prostacyclin (100 micrograms/kg) in rats resulted in a decrease of systolic blood pressure within 2 minutes. Concentrations of cAMP in 15 brain regions and nuclei were determined by radioimmunoassay. In lower brain stem nuclei, such as the nucleus of the solitary tract and the lateral reticula...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90439-5
更新日期：1987-12-07 00:00:00
abstract：AIMS:Hypercholesterolemia is a hazard for increasing susceptibility of the heart to myocardial infarction (MI) by inducing platelet hyperaggregability. Clopidogrel and prasugrel have documented cardioprotective effects in clinical studies. Herein, we investigated whether clopidogrel and prasugrel could protect against ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117429
更新日期：2020-04-15 00:00:00
abstract：:Rats with experimental allergic encephalomyelitis (EAE) induced with myelin or spinal cord show decreases in the content of sulphatides and cerebrosides and increases in the level of esterified cholesterol in the CNS. In this work it is shown that brain sulphatide changes can be obtained by injection of mixtures conta...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90600-2
更新日期：1982-03-08 00:00:00
abstract：AIM:This study investigated the effect of voluntary motor effort during a low-intensity (30% maximal voluntary contraction [MVC]) muscle exercise training program on increasing muscle strength. MATERIALS AND METHODS:Eighteen young and healthy individuals were randomly assigned to one of three groups: high mental effor...
journal_title：Life sciences
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.lfs.2017.04.003
更新日期：2017-06-01 00:00:00
