Abstract：
:The aim of the present work was to study whether physiological doses of melatonin (1nM) modified DNA synthesis in MCF-7 human breast cancer cells. Exponentially growing MCF-7 cells were incubated for 24 h with thymidine (2mM) for blocking mitosis and synchronizing the cell division cycle. Synchronization was assessed by a flow cytometry study which showed that after release from excess thymidine, 82.3% of the cells were in phase G1. Lots of these synchronized cells were pulsed for 1h with [3H]deoxythymidine ([3H]dThy) or [3H]dThy + melatonin, at 0,3,6,9,12,15 or 24 h from the release of the mitotic arrest. The exposition of these synchronized MCF-7 cells to melatonin for only 1h, significantly inhibited [3H]dThy incorporation when it was at 6 or 9 h. after release from mitotic block, at a time when DNA precursor incorporation was the highest and the number of cells in S phase was maximum. We conclude that, at least in part, melatonin antiproliferative effects on MCF-7 cells could be mediated by a reduction of DNA synthesis.
journal_name
Life Scijournal_title
Life sciencesauthors
Cos S,Fernández F,Sánchez-Barceló EJdoi
10.1016/0024-3205(96)00249-4subject
Has Abstractpub_date
1996-05-24 00:00:00pages
2447-53issue
26eissn
0024-3205issn
1879-0631pii
0024320596002494journal_volume
58pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：OBJECTIVE:This study investigated the role of long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) in kidney injury induced by urine-derived sepsis (US). MATERIALS AND METHODS:An Escherichia coli suspension was injected into the distal ureter of adult male Sprague Dawley rats to establish a US model. Lipo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.08.041
更新日期：2018-10-01 00:00:00
abstract：:Interleukin-1 (IL-1) is a monokine which has been demonstrated to produce a variety of seemingly diverse metabolic events including fever, neutrophilia, anorexia, altered mineral metabolism, muscle catabolism, and fibroblast proliferation. Because many of the clinical features of alcoholic hepatitis are metabolic abno...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(86)90554-0
更新日期：1986-10-20 00:00:00
abstract：:Liquid chromatography of porcine duodenal extracts, in conjugation with bioassay on guinea-pig ileum myenteric plexus longitudinal muscle preparation, yielded substances which inhibited electrically-evoked twitches of the preparations, but whose actions could not be reversed by naloxone, an opioid antagonist. The subs...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90439-9
更新日期：1984-12-17 00:00:00
abstract：AIMS:Reactive oxygen species (ROS) are generated in the ischaemic myocardium especially during early reperfusion and affect myocardial function and viability. Monoterpenes have been proposed to play beneficial roles in diverse physiological systems; however, the mechanisms of action remain largely unknown. This study a...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2017.01.008
更新日期：2017-03-01 00:00:00
abstract：:In saturation binding experiments, (+)pentazocine, (+)3-(3-hydroxyphenyl)-N-propylpiperidine (3-PPP), haloperidol and rimcazole did not inhibit the binding of [3H]DTG in a purely competitive fashion. Although Scatchard analysis indicated that [3H]DTG bound to a single site, the inhibition curves of some, but not all, ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90255-n
更新日期：1992-01-01 00:00:00
abstract：:Benzamide riboside (BR) is a novel anticancer agent exhibiting pronounced activity against several human tumor cell lines via the inhibition of inosine 5'-monophosphate dehydrogenase (IMPDH), thereby restricting the biosynthesis of guanylates. Although it has been demonstrated that BR inhibits IMPDH and induces apopto...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.11.026
更新日期：2004-05-28 00:00:00
abstract：:In our previous studies, exposure of mice to millimeter waves (MW) increased the duration of anesthesia caused by either ketamine or chloral hydrate, and this effect was blocked by naloxone. To further characterize the biological effects of MW, we have chosen a new animal model of experimental itch. Male Swiss albino ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00436-6
更新日期：1998-01-01 00:00:00
abstract：:Changes in the activities of protein kinase A (cAMP-dependent), protein kinase C (Ca2+/phospholipid-dependent), and protein kinase M (Ca2+/calmodulin-dependent) in dog heart and liver were studied 4 hr following endotoxin injection. Protein kinases A and C were extracted and partially purified by DEAE-cellulose chroma...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(94)00838-8
更新日期：1994-01-01 00:00:00
abstract：:Antinuclear antibodies (ANA) development was studied in male guinea pigs in response to chronic treatment with procainamide, hydralazine, acetanilide or caffeine. Acetanilide and caffeine have not previously been associated with ANA induction. Fifty-one weanling Hartley guinea pigs were divided into five groups which ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90653-1
更新日期：1982-01-11 00:00:00
abstract：:The discovery and development of new drugs targeting voltage-gated ion channels are important for treating a variety of medical conditions and diseases. Ion channels are molecular nanostructures expressed ubiquitously throughout the whole body, and are involved in many basic physiological processes. Over the years, na...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2011.04.020
更新日期：2011-10-10 00:00:00
abstract：:Thymic hormones have immunomodulatory effects on T cells and hence have been used clinically to restore the immunity of immunodeficient patients as well as to enhance the cellular immunity of cancer patients. Prothymosin alpha, which is a member of the thymic hormone family, has recently been suggested to act as a nuc...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00882-5
更新日期：1997-01-01 00:00:00
abstract：:It has been previously shown in anesthetized pigs that intravenous infusion of 2 microg/h of 17beta-estradiol primarily dilated renal, iliac and coronary circulations, while higher doses of the hormone were required to cause vasodilation also in the mesenteric vascular bed. In the same experimental model, a tonic beta...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00399-9
更新日期：1999-01-01 00:00:00
abstract：:TCDD was assessed as a biological response modifier for increasing MMC cytotoxicity through aryl hydrocarbon receptor (AhR) activation and increasing levels of bioreductive enzymes. Human MCF-7 cells were exposed to TCDD, MMC and combinations thereof under aerobic or hypoxic conditions. Cytotoxicity, enzyme activities...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.07.021
更新日期：2006-02-23 00:00:00
abstract：:Clinical trials with muscarinic agonists or acetylcholine esterase inhibitors for the treatment of Alzheimer's dementia have shown disappointing or equivocal results. An alternative treatment of this disease is the development of drugs which enhance the release of acetylcholine. It is believed, that of the five muscar...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(93)90307-o
更新日期：1993-01-01 00:00:00
abstract：:It was possible to assess that ACE inhibitors bound to two different sites on the enzymes which consist in two homologous domains. Accordingly, it was also shown that testis ACE which consists in a single domain exhibits only one site for inhibitors. The common binding site of both enzymes contains Zn2+, bears the enz...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(92)90489-c
更新日期：1992-01-01 00:00:00
abstract：:We administered a series of alpha-blocking drugs to the nucleus reticularis lateralis (NRL) of the medulla oblongata, the main site for the hypotensive action of clonidine. These experiments were performed on pentobarbital anaesthetized cats. Drugs were injected through a needle which was stereotaxically inserted. Pra...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90566-2
更新日期：1987-03-16 00:00:00
abstract：:Rabbits were made reticulocytotic by repeated bleeding or by injection of phenylhydrazine. Up to reticulocyte levels of 70%, the baseline activity of magnesium-dependent calcium-stimulated adenosine triphosphatase ( ( Ca + Mg)-ATPase, EC 3.6.1.3.) of the red cell plasma membrane was not significantly changed. Maximal ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90622-7
更新日期：1983-08-29 00:00:00
abstract：:Castration increases the central nervous system (CNS) sensitivity to hexobarbital as measured by an EEG-threshold method. Testosterone treatment in doses from 1 to 5 mg/kg/day decreases CNS-sensitivity and restores threshold values in castrated rats to a level found in normal young adult male rats. ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(85)90385-6
更新日期：1985-03-11 00:00:00
abstract：:The flavonoid quercetin exhibited a biphasic effect on calcium and phospholipid-dependent protein kinase (protein kinase C) activity from rat brain and pig thyroid. At a low concentration (10(-7) M) quercetin stimulated the enzyme activity whereas at higher concentrations quercetin was inhibitory. By contrast the synt...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90450-5
更新日期：1989-01-01 00:00:00
abstract：:Previous studies showed that acute treatment with aminophylline (AMPY) significantly elevated maximum thermogenesis and improved cold tolerance in rats and man in severe cold. However, the exact mechanism by which AMPY enhances thermogenesis was unknown. Rats receiving enprofylline (ENPRO) (1.5 and 15 mg/kg, i.p.), a ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(89)90491-8
更新日期：1989-01-01 00:00:00
abstract：:Methodological shortcomings present in elicitation of male sexual reflexes in anesthetized animals. The present study has demonstrated, however, that intraperitoneal (i.p.) injection of p-chloroamphetamine (PCA), an indirect serotonin (5-HT) agonist, elicited simultaneously both penile erection and ejaculation in anes...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00895-x
更新日期：2000-11-10 00:00:00
abstract：AIMS:Our overall objective was to investigate the effect of the adenosine derivative 2',3',5'-tri-O-acetyl-N6-(3-hydroxylaniline) adenosine (WS010117) on AMP-activated protein kinase (AMPK) activation and lipid metabolism and to also assess the underlying mechanisms involved in these processes. MAIN METHODS:HepG2 cell...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2011.09.001
更新日期：2012-01-02 00:00:00
abstract：AIMS:The prognoses of patients with gastric cancer(GC) vary in different stages, which is mainly due to the great differences in tumor and tumor microenvironment. This study is aimed to explore the specific differences. MAIN METHODS:Based on RNAseq-based expression data from The Cancer Genome Atlas database and GSE154...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118402
更新日期：2020-11-15 00:00:00
abstract：:Animal studies suggest nicotine and cannabinoids may significantly enhance the therapeutic value of neuroleptics in motor disorders. This was recently demonstrated in humans by the finding that chewing nicotine gum produced striking relief from tics and other symptoms of Tourette syndrome not controlled by neuroleptic...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/0024-3205(89)90444-x
更新日期：1989-01-01 00:00:00
abstract：:Prenatal exposure to low concentrations of carbon monoxide (CO, 75 and 150 ppm from day 0 to day 20 of gestation), resulting in maternal blood HbCO concentrations equivalent to those maintained by human cigarette smokers, leads to subtle myelin alterations in the sciatic nerve of male rat offspring. The rapid growth s...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00761-x
更新日期：2000-08-25 00:00:00
abstract：:A variety of chromatographic approaches are detailed for enhancement of signal and selectivity for determination of biogenic amines by HPLC with amperometric detection. Microbore HPLC columns offer significant signal enhancement due to the reduced elution volume. Selection of novel surfactants such as N-methyl oleoyl ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(87)90189-5
更新日期：1987-08-17 00:00:00
abstract：:Mycobacterium tuberculosis is the most successful pathogen with multiple mechanisms to subvert host immune response, resulting in insidious disease. There are few studies on whether the bacteria undergo antigenic variation in response to host immune pressure. Studies on T cell epitopes of M. tuberculosis can help us f...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.08.040
更新日期：2018-09-15 00:00:00
abstract：:This study was designed to investigate the effect of diabetic duration on serum concentrations of endogenous inhibitor of nitric oxide synthase N(G), N(G)-asymmetric dimethylarginine (ADMA) in patients and rats with diabetes, and to determine whether elevated endogenous ADMA is implicated in endothelial dysfunction or...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.10.062
更新日期：2005-05-27 00:00:00
abstract：:Site-directed mutagenesis has been used to evaluate the roles of the key aspartate and arginine residues in transmembrane domain three of the muscarinic receptors. The results suggest that the formation of an ionic bond between the Asp carboxylate group and the onium headgroup is essential to anchor acetylcholine in i...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00025-2
更新日期：1995-01-01 00:00:00
abstract：AIMS:We investigated the effects of chemical stimulation of cerebellum fastigial nucleus (FN) on the chronic visceral hypersensitivity (CVH) and its possible mechanism in rats. MAIN METHODS:We stimulated the FN by microinjecting glutamate into the FN, in order to explore whether the cerebellum fastigial nucleus played...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.04.043
更新日期：2018-06-15 00:00:00
