Abstract：
:Site-directed mutagenesis has been used to evaluate the roles of the key aspartate and arginine residues in transmembrane domain three of the muscarinic receptors. The results suggest that the formation of an ionic bond between the Asp carboxylate group and the onium headgroup is essential to anchor acetylcholine in its active, bound conformation in both binary agonist-receptor and ternary agonist-receptor-G-protein complexes, but that secondary, non-productive binding modes, promoted by non-polar forces, may contribute to binary complex formation by other ligands. The positive charge of the arginyl side-chain is central to the recognition, and subsequent activation of G-proteins by the agonist-M1 mAChR complex.
journal_name
Life Scijournal_title
Life sciencesauthors
Hulme EC,Curtis CA,Page KM,Jones PGdoi
10.1016/0024-3205(95)00025-2subject
Has Abstractpub_date
1995-01-01 00:00:00pages
891-8issue
11-12eissn
0024-3205issn
1879-0631pii
0024-3205(95)00025-2journal_volume
56pub_type
杂志文章相关文献
LIFE SCIENCES文献大全abstract：:Liver fibrosis is a global health issue that causes morbidity and mortality with no currently available treatment. It has been shown that low dose paclitaxel (PTX) can stabilize microtubules and inhibit the profibrotic transforming growth factor-beta 1 (TGF-β1) signaling pathway. In this study the effect of treatment ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2018.09.037
更新日期：2018-10-15 00:00:00
abstract：:Immunotherapy with immune checkpoint inhibitors, such as anti-PD-1 drugs, is an area in increasing development for its efficacy and advantages in the treatment of advanced metastatic melanoma. In fact, immunotherapy has been the target of several and recent studies in different types of cancer, namely in melanoma, a g...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2019.117093
更新日期：2020-01-01 00:00:00
abstract：:The survival of osteoblast cells is one of the determinants of the development of osteoporosis in patients with inflamed synovium, such as in rheumatoid arthritis (RA). By means of alkaline phosphatase (ALP) activity and osteocalcin ELISA assay, I have shown that myricetin exhibits a significant induction of different...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.05.026
更新日期：2005-10-21 00:00:00
abstract：:According to recent epidemiological reports, almost 40% of American population use complimentary and alternative medicine (CAM) during their lifetime. Patients detected with HIV or cancer often consume herbal products especially St. John's wort (SJW) for antidepressants in combination with prescription medicines. Such...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/j.lfs.2005.12.010
更新日期：2006-03-27 00:00:00
abstract：:During the myocardial protection induced by ischemic preconditioning a reduction in myocardial metabolism occurs due to activation of the A1 adenosine receptors. This study investigates whether preconditioning changes both resting coronary flow and the magnitude of coronary reactive hyperemia and whether A1 adenosine ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00034-x
更新日期：1999-01-01 00:00:00
abstract：AIMS:The study aimed to investigate whether IL-23 is amplified in monocyte subsets of MP pneumonia and to determine its relevant pathway. MATERIALS AND METHODS:We firstly analyze the IL-23p19 expression in monocyte subgroups in MP pneumonia patients and healthy controls subjects by using flow cytometry. Then, we also ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118149
更新日期：2020-10-01 00:00:00
abstract：:The compound (+)-MR200 [(+)-methyl (1R,2S)-2-{[4-(4-chlorophenyl)-4-hydroxypiperidin-1-yl]methyl}-1-phenylcyclopropanecarboxylate] is a sigma ligand with increased affinity and selectivity compared to the structurally related ligand haloperidol. From the results of a previous study on the modulation of a systemically ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2005.10.005
更新日期：2006-04-18 00:00:00
abstract：:Intestinal mucosal capsaicin-sensitive afferent nerves mediate, in part, the protective mesenteric hyperemia after intraduodenal acidification. Mechanisms associated the sensory neuropeptides, e.g. calcitonin gene-related peptide (CGRP), substance P, and ruthenium red-sensitive cation channels contribute to acid-induc...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(02)02106-9
更新日期：2002-10-18 00:00:00
abstract：:In the present study the effects of Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) on fibroblast growth and activity have been studied. In this regard the AA have evaluated in primary cultures of human gengival normal fibroblasts (PG1 cells): a)-the expression of GM-CSF receptor (GM-CSFR) (alfa unit) on the...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(98)00281-1
更新日期：1998-01-01 00:00:00
abstract：:Recent reports indicate that some imidazole-5-acetic acid derivatives are competitive antagonists of angiotensin II receptors. However, to our knowledge, there is no published information regarding: 1) what constant infusion rate of these non-peptide angiotensin receptor blockers is necessary to effectively antagonize...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(90)90096-a
更新日期：1990-01-01 00:00:00
abstract：:Momordin II, a ribosome-inactivating protein from Momordica charantia seeds, was purified by a procedure involving a series of chromatographies on S-Sepharose, Sephadex G-50, CM-Sepharose, and Red Sepharose columns. Highly purified momordin II inhibited cell-free protein synthesis, released adenine from rat liver ribo...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(99)00389-6
更新日期：1999-01-01 00:00:00
abstract：:Nitric oxide (NO) is a highly reactive free radical that is involved in a variety of different biological process. In recent reports, the putative role of NO in the neuropathogenesis of brain inflammation has been demonstrated. And then the relation between neuronal NO and convulsive seizures induced by virus has been...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2003.08.014
更新日期：2004-01-30 00:00:00
abstract：:18-Hydroxycorticosterone (18-OHB) is a precursor of aldosterone and is the only corticosteroid, other than aldosterone, that is synthesized predominantly in the zona glomerulosa. Administration of the dopamine antagonist, metoclopramide results in parallel rises in plasma 18-OHB and aldosterone levels without affectin...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(83)90157-1
更新日期：1983-12-19 00:00:00
abstract：AIMS:Lithium is a common mood stabilizer to treat bipolar disorder. It has a narrow window of therapeutic action and its mechanism of action and possible side effects are still not fully understood. Lithium is a potent inhibitor of glycogen synthase kinase 3β (GSK-3β). Previous studies indicated that lithium can induce...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2012.07.027
更新日期：2012-09-24 00:00:00
abstract：AIMS:Short-chain acyl-CoA dehydrogenase (SCAD) is a key enzyme in fatty acid oxidation. In the present study we aim to investigate the changes in SCAD between pathological and physiological cardiomyocyte hypertrophy. We also explore the different signaling pathways of pathological and physiological cardiomyocyte hypert...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2015.01.015
更新日期：2015-03-01 00:00:00
abstract：:This review aims to systematically review and synthesize scientific evidence for the influence of air pollution exposure and outdoor exercise on health. We conducted a literature search in the PubMed, Cochrane, EMBASE, and Web of Science for articles that evaluated the combination effect of air pollution exposure and ...
journal_title：Life sciences
pub_type： 杂志文章,meta分析
doi：10.1016/j.lfs.2018.12.036
更新日期：2019-02-01 00:00:00
abstract：:Two male adult rhesus monkeys were used and caged individually. The room was temperature-controlled having a light-dark period of 12/12 hours. The animals were rapidly immobilized and immediately anesthetized with ketamine i. m. (10 mg/kg of body weight). They were bled four times at 15, 30, 45 and 60 mins after the k...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(91)90247-9
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND:Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Cardiac fibrosis is the scarring process occurs commonly with CVDs impairing the function and structure of heart. Herein, we investigated the role of circPAN3 in the pathogenesis of cardiac fibrosis. METHODS:A rat myocardial infarctio...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.118015
更新日期：2020-09-15 00:00:00
abstract：:TNF production has been studied in peritoneal macrophages and splenic T cells of Arctic Yakutian ground squirrel (Citellus Undulatus Pallas) in hibernating and awake animals in winter and in prehibernating autumn as well as in active euthermic spring-summer animals. A high level of TNF production in macrophages of gro...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(00)00698-6
更新日期：2000-01-01 00:00:00
abstract：AIM:To investigate the cytotoxic effect of Peruvoside and mechanism of action in human cancers. MAIN METHODS:Cell viability was measured by MTT assay and the cell cycle arrest was identified by FACS. Real-time qPCR and western blotting studies were performed to identify important gene and protein expressions in the di...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2019.117147
更新日期：2020-01-15 00:00:00
abstract：:Adrenals of young adult male mice kept on a LD 12:12 lighting regimen for three weeks prior to study and harvested at four different circadian stages were incubated for 2 hours with 0.4 IU synthetic ACTH in 2 ml Krebs-Ringer buffer (KR), or with 50, 150, and 450 microM of melatonin in KR containing 0.4 IU ACTH. The ad...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(96)00079-3
更新日期：1996-01-01 00:00:00
abstract：:Transcription factor Gata3 is implicated in the formation of autosomal dominant hypoparathyroidism, sensorineural deafness, and renal anomaly (HDR) syndrome. We pursued to identify the potential Gata3 target genes by profiling the gene expression pattern in E9.5 Gata3-/- mouse embryos. Altogether four independent micr...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.10.054
更新日期：2005-04-15 00:00:00
abstract：:Inhaled antimuscarinics, often called anticholinergics in clinical medicine, are established as first line bronchodilators in COPD. Tiotropium has been developed as a new generation antimuscarinic following ipratropium. Tiotropium is a specific, highly potent antimuscarinic, demonstrating very slow dissociation from m...
journal_title：Life sciences
pub_type： 杂志文章,评审
doi：10.1016/s0024-3205(98)00588-8
更新日期：1999-01-01 00:00:00
abstract：:The effects of melatonin treatment and pituitary transplants on serum total and free cholesterol levels, cholesterol esterification index, phospholipid levels and prolactin, thyroid-stimulating hormone (TSH), thyroxine (T4) and triiodothyronine (T3) levels were examined in rats. Male rats were grafted an anterior pitu...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00613-9
更新日期：1997-01-01 00:00:00
abstract：:Pretreatment of pancreatic acini with 5-hydroxytryptamine (5-HT) reduced the binding of the labeled somatostatin (SS) analogue 125I-Tyr3-SMS to pancreatic acinar membranes. This effect was dependent of the dose of 5-HT used and length of pretreatment. This inhibitory effect of 5-HT was abolished when pancreatic acini ...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/s0024-3205(97)00161-6
更新日期：1997-01-01 00:00:00
abstract：:Growth of P. aeruginosa, slowed by the addition of monofluoromethylornithine, difluoromethylarginine and dicyclohexylammonium sulfate, could be restored by addition of 0.1 mM putrescine plus 0.1 muM spermidine, or 0.1 mM spermidine or 5 mM putrescine by themselves. Lower concentrations of putrescine (0.1 mM - 1 mM) al...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(84)90605-2
更新日期：1984-04-16 00:00:00
abstract：HEADING AIMS:Abdominal aortic aneurysm (AAA) is featured by the growth impediment and apoptosis surge of VSMCs (vascular smooth muscle cells). MicroRNAs (miRNAs) are suggested to affect cellular behaviors including cell growth and apoptosis. This study concentrated on unraveling the emerging role of miR-28-5p in abdomi...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2020.117533
更新日期：2020-07-15 00:00:00
abstract：:Pitavastatin is a 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor that is used for suppressing cholesterol biosynthesis. Previously, we have reported that pitavastatin induces the activation of endothelial nitric oxide synthase (eNOS) and increases nitric oxide (NO) production in vascular endothelial cells (...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/j.lfs.2004.12.003
更新日期：2005-03-25 00:00:00
abstract：:Rats, trained to discriminate 1.5 mg/kg of the hallucinogenic agent 5-methoxy-N,N-dimethyltryptamine (5-OMe DMT) from saline in a two-lever drug discrimination task, were challenged with various doses of the 4-methoxy, 4-methylthio and 5-methylthio derivatives of DMT. The 5-OMe DMT cue was found to generalize to all t...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(82)90463-5
更新日期：1982-02-01 00:00:00
abstract：:Endothelin-1 (ET-1) has been proposed as one of the possible mediators of the vasoconstriction seen following ischemia and reperfusion. We investigated the effect of ischemia and reperfusion on the contractile response of canine renal and iliac arteries to the dihydropyridine-type calcium channel agonist (+/-)Bay K 86...
journal_title：Life sciences
pub_type： 杂志文章
doi：10.1016/0024-3205(95)00267-a
更新日期：1995-01-01 00:00:00
